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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pK224.CAG-Rox-STOP-Rox-GFP-ires-tTA-WPRE (Supernova)

Dre-based Supernova series. Expression vector of Rox-stop-Rox-GFP under the CAG promoter.

Catalog number RDB15055
Resource name pK224.CAG-Rox-STOP-Rox-GFP-ires-tTA-WPRE (Supernova)
Alternative name pK224
Clone info. Dre-based Supernova series. Expression vector of Rox-stop-Rox-GFP under the CAG promoter. Dre-mediated recombination, a Rox-stop-Rox is excised and the downstream GFP is expressed. The structure of the construct, see Supplemetary Fig.8 of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Comment Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pCAGplay (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Depositor|Developer Iwasato, Takuji |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).
            Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Luo, W. et al. Sci. Rep., 6:35747, 2016)。
            付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15055 pK224.CAG-Rox-STOP-Rox-GFP-ires-tTA-WPRE (Supernova) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15055_A7Enp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxCA_F1
            Sequence file: RDB15055_A7Ena.seq check
            >D04276B4_A7En_2_pAxCA_F1_A09_03_ABI24.ab1
                1 CAAAACTTTC TACCATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG GCAACGTGCT
               61 GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCTGC AGTCGACGGT ACCGCGGGCC
              121 CGGGATCTTA ACTTTAAATA ATGCCAATTA TTTAAAGTTA TAACGTAAGC TAGCTAGACC
              181 GGTGTCGACT AAAGCCAAAT AGAAAATTAT TCAGTTCCTG GCTTAAGTTT TTAAAAGTGA
              241 TATTATTTAT TTGGTTGTAA CCAACCAAAA GAATGTAAAT AACTAATACA TAATTATGTT
              301 AGTTTTAAGT TAGCAACAAA TTGATTTTAG CTATATTAGC TACTTGGTTA ATAAATAGAA
              361 TATATTTATT TAAAGATAAT TGCGTTTTTA TTGTCAGGGA GTGAGTTTGC TTAAAAACTC
              421 GTTTAGGTTT GTCCTCCCGA AATTATTTAT TTAAATGCGA TGGAGAGTTG GCGCCGAATC
              481 GAAAACTTTA CGCGCTTAAA AGCACGAGTT GGCATCCCTA ACGCGTAGAT CTTTGTGAAG
              541 GAACCTTACT TCTGTGGTGT GACATAATTG GACAAACTAC CTACAGAGAT TTAAAGCTCT
              601 AAGTAAATAT AAAATTTTTA AGTGTATAAT GTGTTAAACT ACTGATTCTA ATTGTTTGTG
              661 TATTTTAGAT TCCAACCTAT GGAACTGATG AATGGGGAGC AGTGTGGATG CCTTTAATGA
              721 GAAAACCTTG TTTTGCTCAG AAGAAATGCA TCTAGTGATG ATGAGGCTAC TGCTGACTTC
              781 TCAACATTCT ACTCCCTCCA AAAAGAAGAG AAGTAAGAAG AACCCAGACT TCTTCGAATG
              841 CTAAGTTTTT GGAGTCATGG CTGTGGTTTA GTATAGACTC CTTGCTGCTT GGCTATTTAA
              901 CCACAAGAAA AGCCTGCACT GTCTATACAA GAAATTAGAA AATTGGATGT TATGGCTGGC
            961 ATAAGAGATC TAATACGCCA TACACATTGA AGTTACTGGC TTAACCTCCA ACTCCTGT
            //
            Primer: pAxCALNLF1
            Sequence file: RDB15055_A7Enb.seq check
            >D04276B4_A7En_2_pAxCALNLF1_C09_09_ABI24.ab1
                1 CATCAATTAT ATCTTATCAT GTCTGGATCA CTAGTGATCT GGCCGGTAAC TTTAAATAAT
               61 GCCAATTATT TAAAGTTACT CGACGCCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC
              121 GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG
              181 TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC
              241 ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG
              301 TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC
              361 GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC
              421 GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC
              481 TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC
              541 GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC
              601 AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC
              661 GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA
              721 GACCCCAACG AGAAGCGCGA TCACATGGGT CCTGCTGGAG TTCGTGACCG CCGCCCGGGA
              781 TCACTCTCGG CATGGACGAG CTGTACAAGT AAGATATCCT AGATCGAGTT AATTTAAGGA
            841 TTCCGCCCCC CCCCCCCTCT TCCCTTC
            //
            Primer: PRE_rev
            Sequence file: RDB15055_A7Enc.seq check
            >D04276B4_A7En_2_PRE_rev_E09_15_ABI24.ab1
                1 ACTTTCCAAT AGCGTAAAGG AGCAACATAG TTAAGAATAC CAGTCAATCT TTCACAAATT
               61 TTGTAATCCA GAGGTTGAGC GGCCGAAGGG CGAATTCGCG GCCGCTTACT TAGTTACCCG
              121 GGGAGCATGT CAAGGTCAAA ATCGTCAAGA GCGTCAGCAG GCAGCATATC AAGGTCAAAG
              181 TCGTCAAGGG CATCGGCTGG GAGCATGTCT AAGTCAAAAT CGTCAAGGGC GTCGGCCGGC
              241 CCGCCGCTTT CGCACTTTAG CTGTTTCTCC AGGCCACATA TGATTAGTTC CAGGCCGAAA
              301 AGGAAGGCAG GTTCGGCTCC CTGATGGTCG AACAGCTCAA TTGCTTGTCT CAGAAGTGGG
              361 GGCATAGAAT CGGTGGTAGG TGTCTCTCTT TCCTCTTTTG CTACTTGATG CTCCTGATCC
              421 TCCAATACGC AGCCCAGTGT AAAGTGGCCC ACGGCGGACA GAGCGTACAG TGCGTTCTCC
              481 AGGGAGAAGC CTTGCTGACA CAGGAACGCG AGCTGATTTT CCAGGGTTTC GTACTGTTTC
              541 TCTGTTGGGC GGGTGCCGAG ATGCACTTTA GCCCCGTCGC GATGTGAGAG GAGAGCACAG
              601 CGGAATGACT TGGCGTTGTT CCGCAGAAAG TCTTGCCATG ACTCGCCTTC CAGGGGGCAG
              661 AAGTGGGTAT GATGCCTGTC CAGCATCTCG ATTGCCAGGG CATCGAGCAG GGCCCGCTTG
              721 TTCTTCACGT GCCAGTACAG GGTAGGCTGC TCAACTCCCA GCTTTTGAGC GAGTTTCCTT
              781 GTCGTCAGGC CTTCGATACC GACTTCATTG AGTAATTCCA GAGCAGAGTT TATGACTTTG
              841 CTCTTGTCCA GTCTAGACAT CGTGTTTTTC AAAGGAAACC ACGTCCCCGT GGTTCGGGGG
              901 GGCCTAGACG TTTTTTTAAC CCTCGACTAA ACACATGTAA AGCATGTGCA CCGAGCCCCA
              961 GATCAGATCC CATACAATGG GGTACCTTCT GGGCATCCTT TCAGCCCTTG TGATTACGCT
             1021 TGAGAGAGCA TTTTACTCTT TCACACTATC CAACTCACAC GTTGCACTGG GGGATGTGCG
            1081 CTTGCAAGGG TATCCTATCA CGTGCTTGAC GAGCACTGAT CGGCTCTAAG T
            //
            Primer: SV40pro_F_V2
            Sequence file: RDB15055_A7End.seq check
            >D04276B4_A7En_2_SV40pro_F_V2_F09_18_ABI24.ab1
                1 GGAACGCATT TTGGAGGGCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG
               61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
              121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
              181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
              241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
              301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
              361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
              421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
              481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
              541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
              601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
              661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
              721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
              781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
              841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC
              901 CCAACCCTGC CATCACGAGA TTTCGATTCC ACCGCCCGCC CTTCTATGAA AGGTTGGGCT
              961 TTCGGAATCG TTTCCGGACG CCCGGCTGGA TGATCCTCCA GCGCGGATCT CATGCTGGAG
             1021 GTTCCTCGGC CAACCCTAAG GGGGAGGCTA ACTGGAATCA CGATGAGACC ATACCGAGAT
            1081 CCGCGCTATG ACGCCATATA GACGAAT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl