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pK224.CAG-Rox-STOP-Rox-GFP-ires-tTA-WPRE (Supernova) (#RDB15055)

Dre-based Supernova series. Expression vector of Rox-stop-Rox-GFP under the CAG promoter.

Alternative name pK224
Clone info. Dre-based Supernova series. Expression vector of Rox-stop-Rox-GFP under the CAG promoter. Dre-mediated recombination, a Rox-stop-Rox is excised and the downstream GFP is expressed. The structure of the construct, see Supplemetary Fig.8 of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Comment Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pCAGplay (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Depositor|Developer Iwasato, Takuji |
 

Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Luo, W. et al. Sci. Rep., 6:35747, 2016)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15055 pK224.CAG-Rox-STOP-Rox-GFP-ires-tTA-WPRE (Supernova) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pK224.CAG-Rox-STOP-Rox-GFP-ires-tTA-WPRE (Supernova) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15055).

Reference section:

Luo, W., Mizuno, H., Iwata, R., Nakazawa, S., Yasuda, K., Itohara, S., Iwasato, T., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15055_A7Enp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB15055_A7Ena.seq check
>D04276B4_A7En_2_pAxCA_F1_A09_03_ABI24.ab1
    1 CAAAACTTTC TACCATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG GCAACGTGCT
   61 GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCTGC AGTCGACGGT ACCGCGGGCC
  121 CGGGATCTTA ACTTTAAATA ATGCCAATTA TTTAAAGTTA TAACGTAAGC TAGCTAGACC
  181 GGTGTCGACT AAAGCCAAAT AGAAAATTAT TCAGTTCCTG GCTTAAGTTT TTAAAAGTGA
  241 TATTATTTAT TTGGTTGTAA CCAACCAAAA GAATGTAAAT AACTAATACA TAATTATGTT
  301 AGTTTTAAGT TAGCAACAAA TTGATTTTAG CTATATTAGC TACTTGGTTA ATAAATAGAA
  361 TATATTTATT TAAAGATAAT TGCGTTTTTA TTGTCAGGGA GTGAGTTTGC TTAAAAACTC
  421 GTTTAGGTTT GTCCTCCCGA AATTATTTAT TTAAATGCGA TGGAGAGTTG GCGCCGAATC
  481 GAAAACTTTA CGCGCTTAAA AGCACGAGTT GGCATCCCTA ACGCGTAGAT CTTTGTGAAG
  541 GAACCTTACT TCTGTGGTGT GACATAATTG GACAAACTAC CTACAGAGAT TTAAAGCTCT
  601 AAGTAAATAT AAAATTTTTA AGTGTATAAT GTGTTAAACT ACTGATTCTA ATTGTTTGTG
  661 TATTTTAGAT TCCAACCTAT GGAACTGATG AATGGGGAGC AGTGTGGATG CCTTTAATGA
  721 GAAAACCTTG TTTTGCTCAG AAGAAATGCA TCTAGTGATG ATGAGGCTAC TGCTGACTTC
  781 TCAACATTCT ACTCCCTCCA AAAAGAAGAG AAGTAAGAAG AACCCAGACT TCTTCGAATG
  841 CTAAGTTTTT GGAGTCATGG CTGTGGTTTA GTATAGACTC CTTGCTGCTT GGCTATTTAA
  901 CCACAAGAAA AGCCTGCACT GTCTATACAA GAAATTAGAA AATTGGATGT TATGGCTGGC
961 ATAAGAGATC TAATACGCCA TACACATTGA AGTTACTGGC TTAACCTCCA ACTCCTGT
//
Primer: pAxCALNLF1
Sequence file: RDB15055_A7Enb.seq check
>D04276B4_A7En_2_pAxCALNLF1_C09_09_ABI24.ab1
    1 CATCAATTAT ATCTTATCAT GTCTGGATCA CTAGTGATCT GGCCGGTAAC TTTAAATAAT
   61 GCCAATTATT TAAAGTTACT CGACGCCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC
  121 GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG
  181 TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC
  241 ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG
  301 TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC
  361 GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC
  421 GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC
  481 TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC
  541 GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC
  601 AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC
  661 GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA
  721 GACCCCAACG AGAAGCGCGA TCACATGGGT CCTGCTGGAG TTCGTGACCG CCGCCCGGGA
  781 TCACTCTCGG CATGGACGAG CTGTACAAGT AAGATATCCT AGATCGAGTT AATTTAAGGA
841 TTCCGCCCCC CCCCCCCTCT TCCCTTC
//
Primer: PRE_rev
Sequence file: RDB15055_A7Enc.seq check
>D04276B4_A7En_2_PRE_rev_E09_15_ABI24.ab1
    1 ACTTTCCAAT AGCGTAAAGG AGCAACATAG TTAAGAATAC CAGTCAATCT TTCACAAATT
   61 TTGTAATCCA GAGGTTGAGC GGCCGAAGGG CGAATTCGCG GCCGCTTACT TAGTTACCCG
  121 GGGAGCATGT CAAGGTCAAA ATCGTCAAGA GCGTCAGCAG GCAGCATATC AAGGTCAAAG
  181 TCGTCAAGGG CATCGGCTGG GAGCATGTCT AAGTCAAAAT CGTCAAGGGC GTCGGCCGGC
  241 CCGCCGCTTT CGCACTTTAG CTGTTTCTCC AGGCCACATA TGATTAGTTC CAGGCCGAAA
  301 AGGAAGGCAG GTTCGGCTCC CTGATGGTCG AACAGCTCAA TTGCTTGTCT CAGAAGTGGG
  361 GGCATAGAAT CGGTGGTAGG TGTCTCTCTT TCCTCTTTTG CTACTTGATG CTCCTGATCC
  421 TCCAATACGC AGCCCAGTGT AAAGTGGCCC ACGGCGGACA GAGCGTACAG TGCGTTCTCC
  481 AGGGAGAAGC CTTGCTGACA CAGGAACGCG AGCTGATTTT CCAGGGTTTC GTACTGTTTC
  541 TCTGTTGGGC GGGTGCCGAG ATGCACTTTA GCCCCGTCGC GATGTGAGAG GAGAGCACAG
  601 CGGAATGACT TGGCGTTGTT CCGCAGAAAG TCTTGCCATG ACTCGCCTTC CAGGGGGCAG
  661 AAGTGGGTAT GATGCCTGTC CAGCATCTCG ATTGCCAGGG CATCGAGCAG GGCCCGCTTG
  721 TTCTTCACGT GCCAGTACAG GGTAGGCTGC TCAACTCCCA GCTTTTGAGC GAGTTTCCTT
  781 GTCGTCAGGC CTTCGATACC GACTTCATTG AGTAATTCCA GAGCAGAGTT TATGACTTTG
  841 CTCTTGTCCA GTCTAGACAT CGTGTTTTTC AAAGGAAACC ACGTCCCCGT GGTTCGGGGG
  901 GGCCTAGACG TTTTTTTAAC CCTCGACTAA ACACATGTAA AGCATGTGCA CCGAGCCCCA
  961 GATCAGATCC CATACAATGG GGTACCTTCT GGGCATCCTT TCAGCCCTTG TGATTACGCT
 1021 TGAGAGAGCA TTTTACTCTT TCACACTATC CAACTCACAC GTTGCACTGG GGGATGTGCG
1081 CTTGCAAGGG TATCCTATCA CGTGCTTGAC GAGCACTGAT CGGCTCTAAG T
//
Primer: SV40pro_F_V2
Sequence file: RDB15055_A7End.seq check
>D04276B4_A7En_2_SV40pro_F_V2_F09_18_ABI24.ab1
    1 GGAACGCATT TTGGAGGGCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG
   61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
  601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC
  901 CCAACCCTGC CATCACGAGA TTTCGATTCC ACCGCCCGCC CTTCTATGAA AGGTTGGGCT
  961 TTCGGAATCG TTTCCGGACG CCCGGCTGGA TGATCCTCCA GCGCGGATCT CATGCTGGAG
 1021 GTTCCTCGGC CAACCCTAAG GGGGAGGCTA ACTGGAATCA CGATGAGACC ATACCGAGAT
1081 CCGCGCTATG ACGCCATATA GACGAAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045. [link to RRC of NBRP]