Please review the QC test results indicated by icon below as well as clone information before placing your order.

pK224.CAG-Rox-STOP-Rox-GFP-ires-tTA-WPRE (Supernova)

Dre-based Supernova series. Expression vector of Rox-stop-Rox-GFP under the CAG promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016). Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Luo, W. et al. Sci. Rep., 6:35747, 2016)。 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15055_A7Enp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB15055_A7Ena.seq
>D04276B4_A7En_2_pAxCA_F1_A09_03_ABI24.ab1     1 CAAAACTTTC TACCATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG GCAACGTGCT    61 GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCTGC AGTCGACGGT ACCGCGGGCC   121 CGGGATCTTA ACTTTAAATA ATGCCAATTA TTTAAAGTTA TAACGTAAGC TAGCTAGACC   181 GGTGTCGACT AAAGCCAAAT AGAAAATTAT TCAGTTCCTG GCTTAAGTTT TTAAAAGTGA   241 TATTATTTAT TTGGTTGTAA CCAACCAAAA GAATGTAAAT AACTAATACA TAATTATGTT   301 AGTTTTAAGT TAGCAACAAA TTGATTTTAG CTATATTAGC TACTTGGTTA ATAAATAGAA   361 TATATTTATT TAAAGATAAT TGCGTTTTTA TTGTCAGGGA GTGAGTTTGC TTAAAAACTC   421 GTTTAGGTTT GTCCTCCCGA AATTATTTAT TTAAATGCGA TGGAGAGTTG GCGCCGAATC   481 GAAAACTTTA CGCGCTTAAA AGCACGAGTT GGCATCCCTA ACGCGTAGAT CTTTGTGAAG   541 GAACCTTACT TCTGTGGTGT GACATAATTG GACAAACTAC CTACAGAGAT TTAAAGCTCT   601 AAGTAAATAT AAAATTTTTA AGTGTATAAT GTGTTAAACT ACTGATTCTA ATTGTTTGTG   661 TATTTTAGAT TCCAACCTAT GGAACTGATG AATGGGGAGC AGTGTGGATG CCTTTAATGA   721 GAAAACCTTG TTTTGCTCAG AAGAAATGCA TCTAGTGATG ATGAGGCTAC TGCTGACTTC   781 TCAACATTCT ACTCCCTCCA AAAAGAAGAG AAGTAAGAAG AACCCAGACT TCTTCGAATG   841 CTAAGTTTTT GGAGTCATGG CTGTGGTTTA GTATAGACTC CTTGCTGCTT GGCTATTTAA   901 CCACAAGAAA AGCCTGCACT GTCTATACAA GAAATTAGAA AATTGGATGT TATGGCTGGC 961 ATAAGAGATC TAATACGCCA TACACATTGA AGTTACTGGC TTAACCTCCA ACTCCTGT //
Primer: pAxCALNLF1 Sequence file: RDB15055_A7Enb.seq
>D04276B4_A7En_2_pAxCALNLF1_C09_09_ABI24.ab1     1 CATCAATTAT ATCTTATCAT GTCTGGATCA CTAGTGATCT GGCCGGTAAC TTTAAATAAT    61 GCCAATTATT TAAAGTTACT CGACGCCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC   121 GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG   181 TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC   241 ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG   301 TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC   361 GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC   421 GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC   481 TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC   541 GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC   601 AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC   661 GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA   721 GACCCCAACG AGAAGCGCGA TCACATGGGT CCTGCTGGAG TTCGTGACCG CCGCCCGGGA   781 TCACTCTCGG CATGGACGAG CTGTACAAGT AAGATATCCT AGATCGAGTT AATTTAAGGA 841 TTCCGCCCCC CCCCCCCTCT TCCCTTC //
Primer: PRE_rev Sequence file: RDB15055_A7Enc.seq
>D04276B4_A7En_2_PRE_rev_E09_15_ABI24.ab1     1 ACTTTCCAAT AGCGTAAAGG AGCAACATAG TTAAGAATAC CAGTCAATCT TTCACAAATT    61 TTGTAATCCA GAGGTTGAGC GGCCGAAGGG CGAATTCGCG GCCGCTTACT TAGTTACCCG   121 GGGAGCATGT CAAGGTCAAA ATCGTCAAGA GCGTCAGCAG GCAGCATATC AAGGTCAAAG   181 TCGTCAAGGG CATCGGCTGG GAGCATGTCT AAGTCAAAAT CGTCAAGGGC GTCGGCCGGC   241 CCGCCGCTTT CGCACTTTAG CTGTTTCTCC AGGCCACATA TGATTAGTTC CAGGCCGAAA   301 AGGAAGGCAG GTTCGGCTCC CTGATGGTCG AACAGCTCAA TTGCTTGTCT CAGAAGTGGG   361 GGCATAGAAT CGGTGGTAGG TGTCTCTCTT TCCTCTTTTG CTACTTGATG CTCCTGATCC   421 TCCAATACGC AGCCCAGTGT AAAGTGGCCC ACGGCGGACA GAGCGTACAG TGCGTTCTCC   481 AGGGAGAAGC CTTGCTGACA CAGGAACGCG AGCTGATTTT CCAGGGTTTC GTACTGTTTC   541 TCTGTTGGGC GGGTGCCGAG ATGCACTTTA GCCCCGTCGC GATGTGAGAG GAGAGCACAG   601 CGGAATGACT TGGCGTTGTT CCGCAGAAAG TCTTGCCATG ACTCGCCTTC CAGGGGGCAG   661 AAGTGGGTAT GATGCCTGTC CAGCATCTCG ATTGCCAGGG CATCGAGCAG GGCCCGCTTG   721 TTCTTCACGT GCCAGTACAG GGTAGGCTGC TCAACTCCCA GCTTTTGAGC GAGTTTCCTT   781 GTCGTCAGGC CTTCGATACC GACTTCATTG AGTAATTCCA GAGCAGAGTT TATGACTTTG   841 CTCTTGTCCA GTCTAGACAT CGTGTTTTTC AAAGGAAACC ACGTCCCCGT GGTTCGGGGG   901 GGCCTAGACG TTTTTTTAAC CCTCGACTAA ACACATGTAA AGCATGTGCA CCGAGCCCCA   961 GATCAGATCC CATACAATGG GGTACCTTCT GGGCATCCTT TCAGCCCTTG TGATTACGCT  1021 TGAGAGAGCA TTTTACTCTT TCACACTATC CAACTCACAC GTTGCACTGG GGGATGTGCG 1081 CTTGCAAGGG TATCCTATCA CGTGCTTGAC GAGCACTGAT CGGCTCTAAG T //
Primer: SV40pro_F_V2 Sequence file: RDB15055_A7End.seq
>D04276B4_A7En_2_SV40pro_F_V2_F09_18_ABI24.ab1     1 GGAACGCATT TTGGAGGGCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG    61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG   121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG   181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT   241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA   301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG   361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT   421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA   481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG   541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG   601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG   661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT   721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC   781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC   841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC   901 CCAACCCTGC CATCACGAGA TTTCGATTCC ACCGCCCGCC CTTCTATGAA AGGTTGGGCT   961 TTCGGAATCG TTTCCGGACG CCCGGCTGGA TGATCCTCCA GCGCGGATCT CATGCTGGAG  1021 GTTCCTCGGC CAACCCTAAG GGGGAGGCTA ACTGGAATCA CGATGAGACC ATACCGAGAT 1081 CCGCGCTATG ACGCCATATA GACGAAT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl