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pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)

Supernova series, a versatile vector system for single-cell labeling in vivo. Expression vector of loxP-stop-loxP-Cas9 under the CAG promoter.

Catalog number RDB15051
Resource name pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)
Alternative name pK237
Clone info. Supernova series, a versatile vector system for single-cell labeling in vivo. Expression vector of loxP-stop-loxP-Cas9 under the CAG promoter. This clone is used as Supernova-mediated CRISPR/Cas9 vectors. pK237 (RDB15051) do not carry guide oligo, thus used as a control in Supernova-CRISPR/Cas9 experiment. The structure of the construct, see Fig.7 of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pX330 (plasmid)
Selectable markers Amp^r (E. coli).
Depositor|Developer Iwasato, Takuji |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).
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Remarks
The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please refer the Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose).
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RDB15051 pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 20775045.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15051_A7Enp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'Element Reverse
Sequence file: RDB15051_A7Ena.seq check
>D04276A9_A7En_1_SV40pA3'element Reverse_A06_03_ABI24.ab1
    1 GGCTTTAGAG GGCGGAGCTA TGGAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
   61 CTTTTGCTGG CCTTTTGCTC ACATGTGAGG GCCTATTTCC CATGATTCCT TCATATTTGC
  121 ATATACGATA CAAGGCTGTT AGAGAGATAA TTGGAATTAA TTTGACTGTA AACACAAAGA
  181 TATTAGTACA AAATACGTGA CGTAGAAAGT AATAATTTCT TGGGTAGTTT GCAGTTTTAA
  241 AATTATGTTT TAAAATGGAC TATCATATGC TTACCGTAAC TTGAAAGTAT TTCGATTTCT
  301 TGGCTTTATA TATCTTGTGG AAAGGACGAA ACACCGGGTC TTCGAGAAGA CCTGTTTTAG
  361 AGCTAGAAAT AGCAAGTTAA AATAAGGCTA GTCCGTTATC AACTTGAAAA AGTGGCACCG
  421 AGTCGGTGCT TTTTTGTTTT AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTT
  481 TTAGCGCGTG CGCCAATTCT GCAGACAAAT GGCTCTAGAG GTACCCGTTA CATAACTTAC
  541 GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAGTAAC
  601 GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT
  661 GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA
  721 ATGGCCCGCC TGGCATTGTG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGGCAGT
  781 ACATCTACGT ATTAGTCATC GCTATTACCA TGGTCGAGGT GAGCCCCACG TTCTGCTTCA
  841 CTCTCCCCAT CTCGCCCTCC ACCCCAATTT TGTATTAATT TATTTTTTTA ATTATTTTTG
901 TGCACGATGG GGGCGGGGGG GGGGGGGGGG GCTCC
//
Primer: pAxCA_F1
Sequence file: RDB15051_A7Enb.seq check
>D04276A9_A7En_1_pAxCA_F1_D03_12_ABI24.ab1
    1 CGCGGGGTGC TGCTACATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG
   61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCT AATAACTTCG TATAGCATAC
  121 ATTATACGAA GTTATATTAA GGGTTCCGGA TCCTCGGGGA CACCAAATAT GGCGATCTCG
  181 GCCTTTTCGT TTCTTGGAGC TGGGACATGT TTGCCATCGA TCCATCTACC ACCAGAACGG
  241 CCGTTAGATC TGCTGCCACC GTTGTTTCCA CCGAAGAAAC CACCGTTGCC GTAACCACCA
  301 CGACGGTTGT TGCTAAAGAA GCTGCCACCG CCACGGCCAC CGTTGTAGCC GCCGTTGTTG
  361 TTATTGTAGT TGCTACTGTT ATTTCTGGCA CTTCTTGGTT TTCCTCTTAA GTGAGGAGGA
  421 ACATAACCAT TCTCGTTGTT GTCGTTGATG CTTAAATTTT GCACTTGTTC GCTCAGTTCA
  481 GCCATAATAT GAAATGCTTT TCTTGTTGTT CTTACGGAAT ACCACTTGCC ACCTATCACC
  541 ACAACTAACT TTTTCCCGTT CCTCCATCTC TTTTATATTT TTTTTCTCGA GGGATCTTTG
  601 TGAAGGAACC TTACTTCTGT GGTGTGACAT AATTGGACAA ACTACCTACA GAGATTTAAA
  661 GCTCTAAGGT AAATATAAAA TTTTTAAGTG TATAATGTGT TAAACTACTG ATTCTAATTG
  721 TTTGTGTATT TTAGATTCCA ACCTATGGAA CTGATGAATG GGAGCAGTGG TGGAATGCCT
  781 TTAATGAGGA AAACCTGTTT TGCTCAGAAG AAATGCCATC TAGTGATGAT GAGGCTACTG
  841 CTGACTCTCA ACATTCTACT CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC CCCAAGGACT
  901 TTCCTTCAGA ATTGCTAAGT TTTTTGAGTC ATGCTGTGTT TAGTAATAGA ACTCTTGCTT
  961 GCTTTGCTAT TTACACCACA AAGGAAAAAG CTGCACTGCT ATACAAGAAA ATTATGGAAA
 1021 AATATTCTGT AACCCTTTAT AAGTAGGCAT AACAGTTATA ATCATAACAT ACTGTTTTTC
 1081 TACTCCACCA CAGGCATAGA GTGTCTGCTA TATACTATGC TCAAAAATGT GTAACGTTAG
1141 CTTTTTAGTT TGGTAAGGAT AATAACGCTA ATTATTTTTG AATGGTTTAT ATAGTGTGC
//
Primer: pAxCALNLF1
Sequence file: RDB15051_A7Enc.seq check
>D04276A9_A7En_1_pAxCALNLF1_B03_06_ABI24.ab1
    1 TTCAAATCTA TCTTATCATG TCTGGATCTG ACATGGTAAG TAAGCTTGGG CTGCAGGTCG
   61 AGGGACCTAA TAACTTCGTA TAGCATACAT TATACGAAGT TATATGTCGA CGGTACCGCG
  121 GGCCCGGGAT CCACCGGTGC CACCATGGAC TATAAGGACC ACGACGGAGA CTACAAGGAT
  181 CATGATATTG ATTACAAAGA CGATGACGAT AAGATGGCCC CAAAGAAGAA GCGGAAGGTC
  241 GGTATCCACG GAGTCCCAGC AGCCGACAAG AAGTACAGCA TCGGCCTGGA CATCGGCACC
  301 AACTCTGTGG GCTGGGCCGT GATCACCGAC GAGTACAAGG TGCCCAGCAA GAAATTCAAG
  361 GTGCTGGGCA ACACCGACCG GCACAGCATC AAGAAGAACC TGATCGGAGC CCTGCTGTTC
  421 GACAGCGGCG AAACAGCCGA GGCCACCCGG CTGAAGAGAA CCGCCAGAAG AAGATACACC
  481 AGACGGAAGA ACCGGATCTG CTATCTGCAA GAGATCTTCA GCAACGAGAT GGCCAAGGTG
  541 GACGACAGCT TCTTCCACAG ACTGGAAGAG TCCTTCCTGG TGGAAGAGGA TAAGAAGCAC
  601 GAGCGGCACC CCATCTTCGG CAACATCGTG GACGAGGTGG CCTACCACGA GAAGTACCCC
  661 ACCATCTACC ACCTGAGAAA GAAACTGGTG GACAGCACCG ACAAGGCCGA CCTGCGGCTG
  721 ATCTATCTGG CCCTGGCCCA CATGATCAAG TTCCGGGGCC ACTTCCTGAT CGAGGGCGAC
  781 CTGAACCCCG ACAACAGCGA CGTGGACAAG CTGTTCATCC AGCTGGTGCA GACCTACAAC
  841 CAGCTGTTCG AGGAAAACCC CATCAACGCC AGCGGCGTGG ACGCCAAGGC CATCCTGTCT
  901 GCCAGACTGA GCAAGAGCAG ACGGCTGGAA AATCTGATCG CCCAGCTGCC CGGCGAGAAG
  961 AAGAATGGCC TGTTCGGAAA CCTGATTGCC CTGAGCCTGG GCCCTGACCC CCAACTTCAA
 1021 GAGCAACTTC GACCTGACGA GATGCCAAAC TGCAGCTGAG CAGACACCTA CGACGACGAC
 1081 TGACCACTGC TACCAGATCG GCGACCAGGT TACGCCGAAC CTGGTTTCTG CCGGCCAGAT
1141 CTGATCGGAC GTCATTCCTT GCCTGAGAGC GTACAC
//
Primer: BGH_rev2
Sequence file: RDB15051_A7End.seq check
>D04276A9_A7En_1_BGH_rev2_F10_16_ABI24.ab1
    1 CAATAGCGGT TAGGAAGCAC GGGGGAGGGG CAAACAACAG ATGGCTGGCA ACTAGAAGGC
   61 ACAGTCGAGG CTGATCAGCG AGCTCTAGGA ATTCTTACTT TTTCTTTTTT GCCTGGCCGG
  121 CCTTTTTCGT GGCCGCCGGC CTTTTGTCGC CTCCCAGCTG AGACAGGTCG ATCCGTGTCT
  181 CGTACAGGCC GGTGATGCTC TGGTGGATCA GGGTGGCGTC CAGCACCTCT TTGGTGCTGG
  241 TGTACCTCTT CCGGTCGATG GTGGTGTCAA AGTACTTGAA GGCGGCAGGG GCTCCCAGAT
  301 TGGTCAGGGT AAACAGGTGG ATGATATTCT CGGCCTGCTC TCTGATGGGC TTATCCCGGT
  361 GCTTGTTGTA GGCGGACAGC ACTTTGTCCA GATTAGCGTC GGCCAGGATC ACTCTCTTGG
  421 AGAACTCGCT GATCTGCTCG ATGATCTCGT CCAGGTAGTG CTTGTGCTGT TCCACAAACA
  481 GCTGTTTCTG CTCATTATCC TCGGGGGAGC CCTTCAGCTT CTCATAGTGG CTGGCCAGGT
  541 ACAGGAAGTT CACATATTTG GAGGGCAGGG CCAGTTCGTT TCCCTTCTGC AGTTCGCCGG
  601 CAGAGGCCAG CATTCTCTTC CGGCCGTTTT CCAGCTCGAA CAGGGAGTAC TTAGGCAGCT
  661 TGATGATCAG GTCCTTTTTC ACTTCTTTGT AGCCCTTGGC TTCCAGAAAG TCGATGGGAT
  721 TCTTCTCGAA GCTGCTTCTT TCCATGATGG TGATCCCCAG CAGCTCTTTC ACACTCTTCA
  781 GTTTCTTGGA CTTGCCCTTT TCCACTTTGG CCACCACCAG CACAGAATAG GCCACGGTGG
  841 GGCTGTCGAA GCCGCCGTAC TTCTTAGGGT CCCAGTCCTT CTTTCTGGCG ATCAGCTTAT
  901 CGCTGTTCCT CTTGGGCAGG ATAGACTCTT TGCTGAAGCC GCCTGTCTGC ACCTCGGTCT
  961 TTTTCACGAT ATTCACTTGG GGGCATGCTC AGCACTTTCC GGCACGGTGG CAAAATCCCG
 1021 GGCCCTTATC CCCACACGAT CTCCCCGGTT TCGCCGTTTT GTCTCGATCA GAGTGGCCTT
1081 TCTGAATCTC GTCGATTGGT CAGTAAATCT CGTCTGGAAA AAGTTCATGT GATGGA
//
Primer: f1ori_R3
Sequence file: RDB15051e_A7En.seq check
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 20775045.

2021.03.11

GNP_filter3_RDBDEP_html_210308.pl