Please review the QC test results indicated by icon below as well as clone information before placing your order.

pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)

Supernova series, a versatile vector system for single-cell labeling in vivo. Expression vector of loxP-stop-loxP-Cas9 under the CAG promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Remarks	The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Luo, W. et al. Sci. Rep., 6:35747, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
備考	本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15051_A7Enp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'Element Reverse Sequence file: RDB15051_A7Ena.seq
>D04276A9_A7En_1_SV40pA3'element Reverse_A06_03_ABI24.ab1     1 GGCTTTAGAG GGCGGAGCTA TGGAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC    61 CTTTTGCTGG CCTTTTGCTC ACATGTGAGG GCCTATTTCC CATGATTCCT TCATATTTGC   121 ATATACGATA CAAGGCTGTT AGAGAGATAA TTGGAATTAA TTTGACTGTA AACACAAAGA   181 TATTAGTACA AAATACGTGA CGTAGAAAGT AATAATTTCT TGGGTAGTTT GCAGTTTTAA   241 AATTATGTTT TAAAATGGAC TATCATATGC TTACCGTAAC TTGAAAGTAT TTCGATTTCT   301 TGGCTTTATA TATCTTGTGG AAAGGACGAA ACACCGGGTC TTCGAGAAGA CCTGTTTTAG   361 AGCTAGAAAT AGCAAGTTAA AATAAGGCTA GTCCGTTATC AACTTGAAAA AGTGGCACCG   421 AGTCGGTGCT TTTTTGTTTT AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTT   481 TTAGCGCGTG CGCCAATTCT GCAGACAAAT GGCTCTAGAG GTACCCGTTA CATAACTTAC   541 GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAGTAAC   601 GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT   661 GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA   721 ATGGCCCGCC TGGCATTGTG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGGCAGT   781 ACATCTACGT ATTAGTCATC GCTATTACCA TGGTCGAGGT GAGCCCCACG TTCTGCTTCA   841 CTCTCCCCAT CTCGCCCTCC ACCCCAATTT TGTATTAATT TATTTTTTTA ATTATTTTTG 901 TGCACGATGG GGGCGGGGGG GGGGGGGGGG GCTCC //
Primer: pAxCA_F1 Sequence file: RDB15051_A7Enb.seq
>D04276A9_A7En_1_pAxCA_F1_D03_12_ABI24.ab1     1 CGCGGGGTGC TGCTACATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG    61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCT AATAACTTCG TATAGCATAC   121 ATTATACGAA GTTATATTAA GGGTTCCGGA TCCTCGGGGA CACCAAATAT GGCGATCTCG   181 GCCTTTTCGT TTCTTGGAGC TGGGACATGT TTGCCATCGA TCCATCTACC ACCAGAACGG   241 CCGTTAGATC TGCTGCCACC GTTGTTTCCA CCGAAGAAAC CACCGTTGCC GTAACCACCA   301 CGACGGTTGT TGCTAAAGAA GCTGCCACCG CCACGGCCAC CGTTGTAGCC GCCGTTGTTG   361 TTATTGTAGT TGCTACTGTT ATTTCTGGCA CTTCTTGGTT TTCCTCTTAA GTGAGGAGGA   421 ACATAACCAT TCTCGTTGTT GTCGTTGATG CTTAAATTTT GCACTTGTTC GCTCAGTTCA   481 GCCATAATAT GAAATGCTTT TCTTGTTGTT CTTACGGAAT ACCACTTGCC ACCTATCACC   541 ACAACTAACT TTTTCCCGTT CCTCCATCTC TTTTATATTT TTTTTCTCGA GGGATCTTTG   601 TGAAGGAACC TTACTTCTGT GGTGTGACAT AATTGGACAA ACTACCTACA GAGATTTAAA   661 GCTCTAAGGT AAATATAAAA TTTTTAAGTG TATAATGTGT TAAACTACTG ATTCTAATTG   721 TTTGTGTATT TTAGATTCCA ACCTATGGAA CTGATGAATG GGAGCAGTGG TGGAATGCCT   781 TTAATGAGGA AAACCTGTTT TGCTCAGAAG AAATGCCATC TAGTGATGAT GAGGCTACTG   841 CTGACTCTCA ACATTCTACT CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC CCCAAGGACT   901 TTCCTTCAGA ATTGCTAAGT TTTTTGAGTC ATGCTGTGTT TAGTAATAGA ACTCTTGCTT   961 GCTTTGCTAT TTACACCACA AAGGAAAAAG CTGCACTGCT ATACAAGAAA ATTATGGAAA  1021 AATATTCTGT AACCCTTTAT AAGTAGGCAT AACAGTTATA ATCATAACAT ACTGTTTTTC  1081 TACTCCACCA CAGGCATAGA GTGTCTGCTA TATACTATGC TCAAAAATGT GTAACGTTAG 1141 CTTTTTAGTT TGGTAAGGAT AATAACGCTA ATTATTTTTG AATGGTTTAT ATAGTGTGC //
Primer: pAxCALNLF1 Sequence file: RDB15051_A7Enc.seq
>D04276A9_A7En_1_pAxCALNLF1_B03_06_ABI24.ab1     1 TTCAAATCTA TCTTATCATG TCTGGATCTG ACATGGTAAG TAAGCTTGGG CTGCAGGTCG    61 AGGGACCTAA TAACTTCGTA TAGCATACAT TATACGAAGT TATATGTCGA CGGTACCGCG   121 GGCCCGGGAT CCACCGGTGC CACCATGGAC TATAAGGACC ACGACGGAGA CTACAAGGAT   181 CATGATATTG ATTACAAAGA CGATGACGAT AAGATGGCCC CAAAGAAGAA GCGGAAGGTC   241 GGTATCCACG GAGTCCCAGC AGCCGACAAG AAGTACAGCA TCGGCCTGGA CATCGGCACC   301 AACTCTGTGG GCTGGGCCGT GATCACCGAC GAGTACAAGG TGCCCAGCAA GAAATTCAAG   361 GTGCTGGGCA ACACCGACCG GCACAGCATC AAGAAGAACC TGATCGGAGC CCTGCTGTTC   421 GACAGCGGCG AAACAGCCGA GGCCACCCGG CTGAAGAGAA CCGCCAGAAG AAGATACACC   481 AGACGGAAGA ACCGGATCTG CTATCTGCAA GAGATCTTCA GCAACGAGAT GGCCAAGGTG   541 GACGACAGCT TCTTCCACAG ACTGGAAGAG TCCTTCCTGG TGGAAGAGGA TAAGAAGCAC   601 GAGCGGCACC CCATCTTCGG CAACATCGTG GACGAGGTGG CCTACCACGA GAAGTACCCC   661 ACCATCTACC ACCTGAGAAA GAAACTGGTG GACAGCACCG ACAAGGCCGA CCTGCGGCTG   721 ATCTATCTGG CCCTGGCCCA CATGATCAAG TTCCGGGGCC ACTTCCTGAT CGAGGGCGAC   781 CTGAACCCCG ACAACAGCGA CGTGGACAAG CTGTTCATCC AGCTGGTGCA GACCTACAAC   841 CAGCTGTTCG AGGAAAACCC CATCAACGCC AGCGGCGTGG ACGCCAAGGC CATCCTGTCT   901 GCCAGACTGA GCAAGAGCAG ACGGCTGGAA AATCTGATCG CCCAGCTGCC CGGCGAGAAG   961 AAGAATGGCC TGTTCGGAAA CCTGATTGCC CTGAGCCTGG GCCCTGACCC CCAACTTCAA  1021 GAGCAACTTC GACCTGACGA GATGCCAAAC TGCAGCTGAG CAGACACCTA CGACGACGAC  1081 TGACCACTGC TACCAGATCG GCGACCAGGT TACGCCGAAC CTGGTTTCTG CCGGCCAGAT 1141 CTGATCGGAC GTCATTCCTT GCCTGAGAGC GTACAC //
Primer: BGH_rev2 Sequence file: RDB15051_A7End.seq
>D04276A9_A7En_1_BGH_rev2_F10_16_ABI24.ab1     1 CAATAGCGGT TAGGAAGCAC GGGGGAGGGG CAAACAACAG ATGGCTGGCA ACTAGAAGGC    61 ACAGTCGAGG CTGATCAGCG AGCTCTAGGA ATTCTTACTT TTTCTTTTTT GCCTGGCCGG   121 CCTTTTTCGT GGCCGCCGGC CTTTTGTCGC CTCCCAGCTG AGACAGGTCG ATCCGTGTCT   181 CGTACAGGCC GGTGATGCTC TGGTGGATCA GGGTGGCGTC CAGCACCTCT TTGGTGCTGG   241 TGTACCTCTT CCGGTCGATG GTGGTGTCAA AGTACTTGAA GGCGGCAGGG GCTCCCAGAT   301 TGGTCAGGGT AAACAGGTGG ATGATATTCT CGGCCTGCTC TCTGATGGGC TTATCCCGGT   361 GCTTGTTGTA GGCGGACAGC ACTTTGTCCA GATTAGCGTC GGCCAGGATC ACTCTCTTGG   421 AGAACTCGCT GATCTGCTCG ATGATCTCGT CCAGGTAGTG CTTGTGCTGT TCCACAAACA   481 GCTGTTTCTG CTCATTATCC TCGGGGGAGC CCTTCAGCTT CTCATAGTGG CTGGCCAGGT   541 ACAGGAAGTT CACATATTTG GAGGGCAGGG CCAGTTCGTT TCCCTTCTGC AGTTCGCCGG   601 CAGAGGCCAG CATTCTCTTC CGGCCGTTTT CCAGCTCGAA CAGGGAGTAC TTAGGCAGCT   661 TGATGATCAG GTCCTTTTTC ACTTCTTTGT AGCCCTTGGC TTCCAGAAAG TCGATGGGAT   721 TCTTCTCGAA GCTGCTTCTT TCCATGATGG TGATCCCCAG CAGCTCTTTC ACACTCTTCA   781 GTTTCTTGGA CTTGCCCTTT TCCACTTTGG CCACCACCAG CACAGAATAG GCCACGGTGG   841 GGCTGTCGAA GCCGCCGTAC TTCTTAGGGT CCCAGTCCTT CTTTCTGGCG ATCAGCTTAT   901 CGCTGTTCCT CTTGGGCAGG ATAGACTCTT TGCTGAAGCC GCCTGTCTGC ACCTCGGTCT   961 TTTTCACGAT ATTCACTTGG GGGCATGCTC AGCACTTTCC GGCACGGTGG CAAAATCCCG  1021 GGCCCTTATC CCCACACGAT CTCCCCGGTT TCGCCGTTTT GTCTCGATCA GAGTGGCCTT 1081 TCTGAATCTC GTCGATTGGT CAGTAAATCT CGTCTGGAAA AAGTTCATGT GATGGA //
Primer: f1ori_R3 Sequence file: RDB15051e_A7En.seq
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl