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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

Flippase (Flpe)-based Supernova series. Expression vector of FRT-stop-FRT-EGFP under the CAG promoter.

Catalog number RDB15047
Resource name pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)
Alternative name pK068
Clone info. Flippase (Flpe)-based Supernova series. Expression vector of FRT-stop-FRT-EGFP under the CAG promoter. Flpe-mediated recombination, a FRT-stop-FRT is excised and the downstream EGFP is expressed. The structure of the construct, see Supplemetary Fig.7 of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Comment Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pK029 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Depositor|Developer Iwasato, Takuji |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).
            Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Luo, W. et al. Sci. Rep., 6:35747, 2016)。
            付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15047 pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB15047zzt01.seq compiled by us

            Original reference

            original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045.

            Further references such as user reports and related articles (go to bottom)

            Featured content


            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15047_A7Enp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxCALNLF1
            Region: insert 5'(FRT,EGFP 5')
            Sequence file: RDB15047_A7Ene.seq check
            >D04276A5_A7En_1_pAxCALNLF1_C03_09_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNGT CTGGNNCTGN NNTGGTAAGT AAGCTTGGGC TGCAGGTCGA
               61 GGGACCTAGA AGTTCCTATA CTTTCTAGAG AATAGGAACT TCATGTCGAC GCCACCATGG
              121 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG
              181 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA
              241 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG
              301 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC
              361 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA
              421 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA
              481 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC
              541 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA
              601 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC
              661 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
              721 TGANCACCCA GTCCGCCCTG ANCAAAGACC CCAACGAGAA GCGCGATCAC ATGNNCCTGC
              781 TGGAGTTCGT GACCGCCGCC GGGNTCACTC TCGGCATGGA CGANCTGTAC AAGTAANANA
              841 TCCTANATCG ANTTNATTNA NNNTTCCGCC CCCCCCCCCN CTCCCTCCCC CCCCCCCTNN
              901 NGTTACNGGN CNAANCCGCT NNNNNTANNN NCNNGNGTGC GTTNGNCTAT ATGNTATTTT
            961 NCNCCATNNT NNNNNNNTTT NNNNNNNNNN
            //
            Primer: mVenus-M_F
            Region: insert 3'(EGFP 3'),IRES
            Sequence file: RDB15047_A7Enf.seq check
            >15047_15047_B2CgF_1_mVenus-M_F_E01_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN NNNNTTCNAA GATCCGCCAC AACATCGAGG
               61 ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG
              121 TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG
              181 AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA
              241 TGGACGAGCT GTACAAGTAA GATATCCTAG ATCGAGTTAA TTAAGGATTC CGCCCCCCCC
              301 CCTCTCCCTC CCCCCCCCCT AACGTTACTG GNNGAAGCCG CTTNNNNNNN GGNCGGTGTG
              361 CGNTTGNCNA NNNNNNNNNT TNNNNCATAT TGNCNTCNTT TGNNNATGNG AGGGCNNNNN
            421 NNNNN
            //
            Primer: pAxCA_F1
            Region: FRT,small t intron
            Sequence file: RDB15047_A7Eng.seq check
            >D04276A5_A7En_1_pAxCA_F1_A03_03_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNTGCCTT CTTCTTTTTC CNACNGCTCC TGGGCAACGT
               61 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC TAGAAGTTCC TATACTTTCT
              121 AGAGAATAGG AACTTCATTA AGGGTTCCGG ATCCTCGGGG ACACCAAATA TGGCGATCTC
              181 GGCCTTTTCG TTTCTTGGAG CTGGGACATG TTTGCCATCG ATCCATCTAC CACCAGAACG
              241 GCCGTTAGAT CTGCTGCCAC CGTTGTTTCC ACCGAAGAAA CCACCGTTGC CGTAACCACC
              301 ACGACGGTTG TTGCTAAAGA AGCTGCCACC GCCACGGCCA CCGTTGTAGC CGCCGTTGTT
              361 GTTATTGTAG TTGCTACTGT TATTTCTGGC ACTTCTTGGT TTTCCTCTTA AGTGAGGAGG
              421 AACATAACCA TTCTCGTTGT TGTCGTTGAT GCTTAAATTT TGCACTTGTT CGCTCAGTTC
              481 AGCCATAATA TGAAATGCTT TTCTTGTTGT TCTTACGGAA TACCACTTGC CACCTATCAC
              541 CACAACTAAC TTTTTCCCGT TCCTCCATCT CTTTTATATT TTTTTTCTCG AGGGATCTTT
              601 GTGAAGGAAC CTTACTTCTG TGGTGTGACA TAATTGGACA AACTACCTAC AGAGATTTAA
              661 AGCTCTAAGG TAAATATAAA ATTTTTAAGT GTATAATGTG TTAAACTACT GATTCTAATT
              721 GTTTGTGTAT TTTAGATTCC AACCTATGGA ACTGATGAAT GGGAGCAGTG GTGGAATGCC
              781 TTTAATGANG AAAANCTGTT TTGCTCANAA GAAATGCCNT CTAGTGATGA NGANGCTACT
              841 GCTGANTCTC AACATTCTAC TCCTCCNAAA AAGAAGANAA AGGTANAANA NCCNNANNNA
              901 CTTTCCNNCN NAANTNGCTN ANTTTTTTNN ANNCNTGCTN NNNTTANNNN NANNNNNNNN
            961 GNTNGNTTNG CNNNTTNCNN NNNNNNNN
            //
            Primer: pAxEF R2
            Region: SV40 pA,SV40 NLS,small t intron
            Sequence file: RDB15047_A7Enh.seq check
            >D04276A5_A7En_1_pAxEF R2_D03_12_ABI24.ab1
                1 NNNNNNNNNN NNNGNTTNAN TTGTGANNTT GTGNNGCTAT TGCTTTATTT GTAACCATTA
               61 TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG
              121 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT
              181 ATGATCTCTA GTCAAGGCAC TATACATCAA ATATTCCTTA TTAACCCCTT TACAAATTAA
              241 AAAGCTAAAG GTACACAATT TTTGAGCATA GTTATTAATA GCAGACACTC TATGCCTGTG
              301 TGGAGTAAGA AAAAACAGTA TGTTATGATT ATAACTGTTA TGCCTACTTA TAAAGGTTAC
              361 AGAATATTTT TCCATAATTT TCTTGTATAG CAGTGCAGCT TTTTCCTTTG TGGTGTAAAT
              421 AGCAAAGCAA GCAAGAGTTC TATTACTAAA CACAGCATGA CTCAAAAAAC TTAGCAATTC
              481 TGAAGGAAAG TCCTTGGGGT CTTCTACCTT TCTCTTCTTT TTTGGAGGAG TAGAATGTTG
              541 AGAGTCAGCA GTAGCCTCAT CATCACTAGA TGGCATTTCT TCTGAGCAAA ACAGGTTTTC
              601 CTCATTAAAG GCATTCCACC ACTGCTCCCA TTCATCAGTT CCATAGGTTG GAATCTAAAA
              661 TACACAAACA ATTAGAATCA GTAGTTTAAC ACATTATACA CTTAAAAATT TTATATTTAC
              721 CTTAGAGCTT TAAATCTCTG TAGGTAGTTT GTCCAATTAT GTCACACCAC AGAAGTAAGG
              781 TTCCTTCACA AAGATCCCTC GAGAAAAAAA ATATAAAAGA GATGGAGGAN CNGGGAAAAA
              841 NNTAGTTGNG GNGATAGGTG GNANGTGGTA TTCCGNANNA ANANCNAGAA AAGCNTTTCA
              901 TATTNNNGCT GNNNGANCGN NNNNGTGCAA AATTTNNNCA TCNNCGANNN CNNCNAGNNT
            961 NNNNANGTNN NNNNNNNN
            //
            Primer: IRES-F
            Region: tTA Advanced
            Sequence file: RDB15047_A7Eni.seq check
            >15047_15047_B2CgF_1_IRES-F_D01_04_ABI08.ab1
                1 NNNNNNNNNN NNNNGTCGAG GTNNNNNNAN NNCTAGGCCC CCCGAACCAC GGGGNCGTGG
               61 TTTCCTTTGA AAAACACGAT GTCTAGACTG GACAAGAGCA AAGTCATAAA CTCTGCTCTG
              121 GAATTACTCA ATGAAGTCGG TATCGAAGGC CTGACGACAA GGAAACTCGC TCAAAAGCTG
              181 GGAGTTGAGC AGCCTACCCT GTACTGGCAC GTGAAGAACA AGCGGGCCCT GCTCGATGCC
              241 CTGGCAATCG AGATGCTGGA CAGGCATCAT ACCCACTTCT GCCCCCTGGA AGGCGAGTCA
              301 TGGCAAGACT TTCTGCGGAA CAACGCCAAG TCATTCCGCT GTGCTCTCCT CTCACATCGC
              361 GACGGGGCTA AAGTGCATCT CGGCACCCGC CCAACAGAGA AACAGTACGA AACCCTGGAA
              421 AATCAGCTCG CGTTCCTGTG TCAGCAAGGC TTCTCCCTGG AGAACGCACT GTACGCTCTG
              481 TCCGCCGTGG GCCACTTTAC ACTGGGCTGC GTATTGGAGG ATCAGGAGCA TCAAGTAGCA
              541 AAAGAGGAAA GAGAGACACC TACCACCGAT TCTATGCCCC CACTTCTGAG ACAAGCAATT
              601 GAGCTGTTCG ACCATCAGGG AGCCGAACCT GCCTTCCTTT TCGGCCTGGA ACTAATCATA
              661 TGTGGCCTGG AGAAACAGCT AAAGTGCGAA AGCGGCGGGC CGGCCGACGC CCTTGACGAT
              721 TTTGACTTAN ACATGCTCCC AGCCGATGCC CTTGACGACT TTGACCTTGA TATGCTGCCT
              781 GCTGACGCTC TTGACGATTT TGANCTTGAC ATGCTCCCCG GGTAACTAAG TAAGCGGCCG
              841 CNAATTCGCC CTTCGGCCGC TCAACCTCTG GATTANAAAA NTTGTGAAAG ANTGACTGGN
              901 ATTCNTNACT ATGTTGCTCC TTTTACGCTA TGNNNATACG CTGCTTTAAT GNCNTTTGNA
            961 NCATGCNNTN GCTNNCNNTA TGGNTTTCNT TTTNNNNCNN NNNNNNNN
            //
            Primer: PRE_rev
            Region: WPRE,tTA Advanced
            Sequence file: RDB15047_A7Enj.seq check
            >D04276A5_A7En_1_PRE_rev_E03_15_ABI24.ab1
                1 NNNNNNNNNN NNNNGNNNNN GAGCNNCATA GTTAAGAATA CCAGTCAATC TTTCACAAAT
               61 TTTGTAATCC AGAGGTTGAG CGGCCGAAGG GCGAATTCGC GGCCGCTTAC TTAGTTACCC
              121 GGGGAGCATG TCAAGGTCAA AATCGTCAAG AGCGTCAGCA GGCAGCATAT CAAGGTCAAA
              181 GTCGTCAAGG GCATCGGCTG GGAGCATGTC TAAGTCAAAA TCGTCAAGGG CGTCGGCCGG
              241 CCCGCCGCTT TCGCACTTTA GCTGTTTCTC CAGGCCACAT ATGATTAGTT CCAGGCCGAA
              301 AAGGAAGGCA GGTTCGGCTC CCTGATGGTC GAACAGCTCA ATTGCTTGTC TCAGAAGTGG
              361 GGGCATAGAA TCGGTGGTAG GTGTCTCTCT TTCCTCTTTT GCTACTTGAT GCTCCTGATC
              421 CTCCAATACG CAGCCCAGTG TAAAGTGGCC CACGGCGGAC AGAGCGTACA GTGCGTTCTC
              481 CAGGGAGAAG CCTTGCTGAC ACAGGAACGC GAGCTGATTT TCCAGGGTTT CGTACTGTTT
              541 CTCTGTTGGG CGGGTGCCGA GATGCACTTT AGCCCCGTCG CGATGTGAGA GGAGAGCACA
              601 GCGGAATGAC TTGGCGTTGT TCCGCAGAAA GTCTTGCCAT GACTCGCCTT CCAGGGGGCA
              661 GAAGTGGGTA TGATGCCTGT CCAGCATCTC GATTGCCAGG GCATCGAGCA NGGCCCGCTT
              721 GTTCTTCACG TGCCAGTACA GGGTANGCTG CTCAACTCCC AGCTTTTGAG CGAGTTTCCT
              781 TGTCGTCAGG NCTTCNATAN CGACTTCNTT GAGTANTTCC NGANCANANT TTATGANTTT
              841 GCTCTTGNCC AGTCTAGANA TCGNGTTTTT CAAANGAAAN NACGNNCCNN NGNNTNNNGG
              901 GGGNCNANAN GNTTTTTTTN NNNNCNNNTA AANNCNTGTA AANNNNGNNN NNNNANNNCC
            961 NNNNNNNNNN
            //
            Primer: SV40pro_ori_F
            Region: SV40 pro_ori,NeoR_KanR
            Sequence file: RDB15047_A7Enk.seq check
            >15047_15047_B2CgF_1_SV40pro_ori_F_F01_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN CTNNNNNTAN TCNNGAAGTA GTGAGGAGGC TTTTTTGGAG
               61 GCCTAGGCTT TTGCAAAGAT CGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA
              121 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC
              181 TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG
              241 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAAGACGAG
              301 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT
              361 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG
              421 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG
              481 CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA
              541 GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG
              601 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA CGGCGAGGAT
              661 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT
              721 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGANC GCTATCAGGA CNTANCGTTG
              781 GCTACCCGTG ATATTGCTGA AGANCTTGNC GGCGAATGGG CTGNNCGCTT CCTCNTGCTT
              841 TACNGTATCG CCGCTCCNGA TTCNCANCGC ATCGCCTTCT ATCNNCTTCT TGACNANTTC
              901 TTCTGANCGG GANTCNGGGG TNNNAANNGA CCGNNCNAGC GACGCCNNNN NNNNCCNTNN
            961 NGANNNTNCN NNTNNNNNNN NCNNNCNTCN NNNANNNNNN GNNNTNNNNN NNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl