Please review the QC test results indicated by icon below as well as clone information before placing your order.

pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

Flippase (Flpe)-based Supernova series. Expression vector of FRT-stop-FRT-EGFP under the CAG promoter.

Sequence (full)	RDB15047zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016). Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

Ordering

Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Information of Request for Distribution.[link]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Luo, W. et al. Sci. Rep., 6:35747, 2016)。 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

Tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15047_A7Enp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCALNLF1 (Pr0166) Region: insert 5'(FRT,EGFP 5') Sequence file: RDB15047_A7Ene.seq
>D04276A5_A7En_1_pAxCALNLF1_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGT CTGGNNCTGN NNTGGTAAGT AAGCTTGGGC TGCAGGTCGA    61 GGGACCTAGA AGTTCCTATA CTTTCTAGAG AATAGGAACT TCATGTCGAC GCCACCATGG   121 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG   181 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA   241 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG   301 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC   361 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA   421 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA   481 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC   541 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA   601 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC   661 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC   721 TGANCACCCA GTCCGCCCTG ANCAAAGACC CCAACGAGAA GCGCGATCAC ATGNNCCTGC   781 TGGAGTTCGT GACCGCCGCC GGGNTCACTC TCGGCATGGA CGANCTGTAC AAGTAANANA   841 TCCTANATCG ANTTNATTNA NNNTTCCGCC CCCCCCCCCN CTCCCTCCCC CCCCCCCTNN   901 NGTTACNGGN CNAANCCGCT NNNNNTANNN NCNNGNGTGC GTTNGNCTAT ATGNTATTTT 961 NCNCCATNNT NNNNNNNTTT NNNNNNNNNN //
Primer: mVenus-M_F (Pr0574) Region: insert 3'(EGFP 3'),IRES Sequence file: RDB15047_A7Enf.seq
>15047_15047_B2CgF_1_mVenus-M_F_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN NNNNTTCNAA GATCCGCCAC AACATCGAGG    61 ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG   121 TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG   181 AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA   241 TGGACGAGCT GTACAAGTAA GATATCCTAG ATCGAGTTAA TTAAGGATTC CGCCCCCCCC   301 CCTCTCCCTC CCCCCCCCCT AACGTTACTG GNNGAAGCCG CTTNNNNNNN GGNCGGTGTG   361 CGNTTGNCNA NNNNNNNNNT TNNNNCATAT TGNCNTCNTT TGNNNATGNG AGGGCNNNNN 421 NNNNN //
Primer: pAxCA_F1 (Pr0164) Region: FRT,small t intron Sequence file: RDB15047_A7Eng.seq
>D04276A5_A7En_1_pAxCA_F1_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNTGCCTT CTTCTTTTTC CNACNGCTCC TGGGCAACGT    61 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC TAGAAGTTCC TATACTTTCT   121 AGAGAATAGG AACTTCATTA AGGGTTCCGG ATCCTCGGGG ACACCAAATA TGGCGATCTC   181 GGCCTTTTCG TTTCTTGGAG CTGGGACATG TTTGCCATCG ATCCATCTAC CACCAGAACG   241 GCCGTTAGAT CTGCTGCCAC CGTTGTTTCC ACCGAAGAAA CCACCGTTGC CGTAACCACC   301 ACGACGGTTG TTGCTAAAGA AGCTGCCACC GCCACGGCCA CCGTTGTAGC CGCCGTTGTT   361 GTTATTGTAG TTGCTACTGT TATTTCTGGC ACTTCTTGGT TTTCCTCTTA AGTGAGGAGG   421 AACATAACCA TTCTCGTTGT TGTCGTTGAT GCTTAAATTT TGCACTTGTT CGCTCAGTTC   481 AGCCATAATA TGAAATGCTT TTCTTGTTGT TCTTACGGAA TACCACTTGC CACCTATCAC   541 CACAACTAAC TTTTTCCCGT TCCTCCATCT CTTTTATATT TTTTTTCTCG AGGGATCTTT   601 GTGAAGGAAC CTTACTTCTG TGGTGTGACA TAATTGGACA AACTACCTAC AGAGATTTAA   661 AGCTCTAAGG TAAATATAAA ATTTTTAAGT GTATAATGTG TTAAACTACT GATTCTAATT   721 GTTTGTGTAT TTTAGATTCC AACCTATGGA ACTGATGAAT GGGAGCAGTG GTGGAATGCC   781 TTTAATGANG AAAANCTGTT TTGCTCANAA GAAATGCCNT CTAGTGATGA NGANGCTACT   841 GCTGANTCTC AACATTCTAC TCCTCCNAAA AAGAAGANAA AGGTANAANA NCCNNANNNA   901 CTTTCCNNCN NAANTNGCTN ANTTTTTTNN ANNCNTGCTN NNNTTANNNN NANNNNNNNN 961 GNTNGNTTNG CNNNTTNCNN NNNNNNNN //
Primer: pAxEF R2 (Pr0059) Region: SV40 pA,SV40 NLS,small t intron Sequence file: RDB15047_A7Enh.seq
>D04276A5_A7En_1_pAxEF R2_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNGNTTNAN TTGTGANNTT GTGNNGCTAT TGCTTTATTT GTAACCATTA    61 TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG   121 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT   181 ATGATCTCTA GTCAAGGCAC TATACATCAA ATATTCCTTA TTAACCCCTT TACAAATTAA   241 AAAGCTAAAG GTACACAATT TTTGAGCATA GTTATTAATA GCAGACACTC TATGCCTGTG   301 TGGAGTAAGA AAAAACAGTA TGTTATGATT ATAACTGTTA TGCCTACTTA TAAAGGTTAC   361 AGAATATTTT TCCATAATTT TCTTGTATAG CAGTGCAGCT TTTTCCTTTG TGGTGTAAAT   421 AGCAAAGCAA GCAAGAGTTC TATTACTAAA CACAGCATGA CTCAAAAAAC TTAGCAATTC   481 TGAAGGAAAG TCCTTGGGGT CTTCTACCTT TCTCTTCTTT TTTGGAGGAG TAGAATGTTG   541 AGAGTCAGCA GTAGCCTCAT CATCACTAGA TGGCATTTCT TCTGAGCAAA ACAGGTTTTC   601 CTCATTAAAG GCATTCCACC ACTGCTCCCA TTCATCAGTT CCATAGGTTG GAATCTAAAA   661 TACACAAACA ATTAGAATCA GTAGTTTAAC ACATTATACA CTTAAAAATT TTATATTTAC   721 CTTAGAGCTT TAAATCTCTG TAGGTAGTTT GTCCAATTAT GTCACACCAC AGAAGTAAGG   781 TTCCTTCACA AAGATCCCTC GAGAAAAAAA ATATAAAAGA GATGGAGGAN CNGGGAAAAA   841 NNTAGTTGNG GNGATAGGTG GNANGTGGTA TTCCGNANNA ANANCNAGAA AAGCNTTTCA   901 TATTNNNGCT GNNNGANCGN NNNNGTGCAA AATTTNNNCA TCNNCGANNN CNNCNAGNNT 961 NNNNANGTNN NNNNNNNN //
Primer: IRES-F (Pr0036) Region: tTA Advanced Sequence file: RDB15047_A7Eni.seq
>15047_15047_B2CgF_1_IRES-F_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNGTCGAG GTNNNNNNAN NNCTAGGCCC CCCGAACCAC GGGGNCGTGG    61 TTTCCTTTGA AAAACACGAT GTCTAGACTG GACAAGAGCA AAGTCATAAA CTCTGCTCTG   121 GAATTACTCA ATGAAGTCGG TATCGAAGGC CTGACGACAA GGAAACTCGC TCAAAAGCTG   181 GGAGTTGAGC AGCCTACCCT GTACTGGCAC GTGAAGAACA AGCGGGCCCT GCTCGATGCC   241 CTGGCAATCG AGATGCTGGA CAGGCATCAT ACCCACTTCT GCCCCCTGGA AGGCGAGTCA   301 TGGCAAGACT TTCTGCGGAA CAACGCCAAG TCATTCCGCT GTGCTCTCCT CTCACATCGC   361 GACGGGGCTA AAGTGCATCT CGGCACCCGC CCAACAGAGA AACAGTACGA AACCCTGGAA   421 AATCAGCTCG CGTTCCTGTG TCAGCAAGGC TTCTCCCTGG AGAACGCACT GTACGCTCTG   481 TCCGCCGTGG GCCACTTTAC ACTGGGCTGC GTATTGGAGG ATCAGGAGCA TCAAGTAGCA   541 AAAGAGGAAA GAGAGACACC TACCACCGAT TCTATGCCCC CACTTCTGAG ACAAGCAATT   601 GAGCTGTTCG ACCATCAGGG AGCCGAACCT GCCTTCCTTT TCGGCCTGGA ACTAATCATA   661 TGTGGCCTGG AGAAACAGCT AAAGTGCGAA AGCGGCGGGC CGGCCGACGC CCTTGACGAT   721 TTTGACTTAN ACATGCTCCC AGCCGATGCC CTTGACGACT TTGACCTTGA TATGCTGCCT   781 GCTGACGCTC TTGACGATTT TGANCTTGAC ATGCTCCCCG GGTAACTAAG TAAGCGGCCG   841 CNAATTCGCC CTTCGGCCGC TCAACCTCTG GATTANAAAA NTTGTGAAAG ANTGACTGGN   901 ATTCNTNACT ATGTTGCTCC TTTTACGCTA TGNNNATACG CTGCTTTAAT GNCNTTTGNA 961 NCATGCNNTN GCTNNCNNTA TGGNTTTCNT TTTNNNNCNN NNNNNNNN //
Primer: PRE_rev (Pr0250) Region: WPRE,tTA Advanced Sequence file: RDB15047_A7Enj.seq
>D04276A5_A7En_1_PRE_rev_E03_15_ABI24.ab1     1 NNNNNNNNNN NNNNGNNNNN GAGCNNCATA GTTAAGAATA CCAGTCAATC TTTCACAAAT    61 TTTGTAATCC AGAGGTTGAG CGGCCGAAGG GCGAATTCGC GGCCGCTTAC TTAGTTACCC   121 GGGGAGCATG TCAAGGTCAA AATCGTCAAG AGCGTCAGCA GGCAGCATAT CAAGGTCAAA   181 GTCGTCAAGG GCATCGGCTG GGAGCATGTC TAAGTCAAAA TCGTCAAGGG CGTCGGCCGG   241 CCCGCCGCTT TCGCACTTTA GCTGTTTCTC CAGGCCACAT ATGATTAGTT CCAGGCCGAA   301 AAGGAAGGCA GGTTCGGCTC CCTGATGGTC GAACAGCTCA ATTGCTTGTC TCAGAAGTGG   361 GGGCATAGAA TCGGTGGTAG GTGTCTCTCT TTCCTCTTTT GCTACTTGAT GCTCCTGATC   421 CTCCAATACG CAGCCCAGTG TAAAGTGGCC CACGGCGGAC AGAGCGTACA GTGCGTTCTC   481 CAGGGAGAAG CCTTGCTGAC ACAGGAACGC GAGCTGATTT TCCAGGGTTT CGTACTGTTT   541 CTCTGTTGGG CGGGTGCCGA GATGCACTTT AGCCCCGTCG CGATGTGAGA GGAGAGCACA   601 GCGGAATGAC TTGGCGTTGT TCCGCAGAAA GTCTTGCCAT GACTCGCCTT CCAGGGGGCA   661 GAAGTGGGTA TGATGCCTGT CCAGCATCTC GATTGCCAGG GCATCGAGCA NGGCCCGCTT   721 GTTCTTCACG TGCCAGTACA GGGTANGCTG CTCAACTCCC AGCTTTTGAG CGAGTTTCCT   781 TGTCGTCAGG NCTTCNATAN CGACTTCNTT GAGTANTTCC NGANCANANT TTATGANTTT   841 GCTCTTGNCC AGTCTAGANA TCGNGTTTTT CAAANGAAAN NACGNNCCNN NGNNTNNNGG   901 GGGNCNANAN GNTTTTTTTN NNNNCNNNTA AANNCNTGTA AANNNNGNNN NNNNANNNCC 961 NNNNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR_KanR Sequence file: RDB15047_A7Enk.seq
>15047_15047_B2CgF_1_SV40pro_ori_F_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN CTNNNNNTAN TCNNGAAGTA GTGAGGAGGC TTTTTTGGAG    61 GCCTAGGCTT TTGCAAAGAT CGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA   121 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC   181 TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG   241 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAAGACGAG   301 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT   361 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG   421 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG   481 CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA   541 GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG   601 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA CGGCGAGGAT   661 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT   721 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGANC GCTATCAGGA CNTANCGTTG   781 GCTACCCGTG ATATTGCTGA AGANCTTGNC GGCGAATGGG CTGNNCGCTT CCTCNTGCTT   841 TACNGTATCG CCGCTCCNGA TTCNCANCGC ATCGCCTTCT ATCNNCTTCT TGACNANTTC   901 TTCTGANCGG GANTCNGGGG TNNNAANNGA CCGNNCNAGC GACGCCNNNN NNNNCCNTNN 961 NGANNNTNCN NNTNNNNNNN NCNNNCNTCN NNNANNNNNN GNNNTNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

HTML_filter_RDB_240326.pl