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pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

Flippase (Flpe)-based Supernova series. Expression vector of FRT-stop-FRT-EGFP under the CAG promoter.

Catalog number RDB15047
Resource name pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)
Alternative name pK068
Clone info. Flippase (Flpe)-based Supernova series. Expression vector of FRT-stop-FRT-EGFP under the CAG promoter. Flpe-mediated recombination, a FRT-stop-FRT is excised and the downstream EGFP is expressed. The structure of the construct, see Supplemetary Fig.7 of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pK029 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Depositor|Developer Iwasato, Takuji |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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RDB15047 pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 20775045.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15047_A7Enp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB15047_A7Ena.seq check
>D04276A5_A7En_1_pAxCA_F1_A03_03_ABI24.ab1
    1 TGAACGAATC TTGCTACATG TTCATGCCTT CTTCTTTTTC CTACAGCTCC TGGGCAACGT
   61 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC TAGAAGTTCC TATACTTTCT
  121 AGAGAATAGG AACTTCATTA AGGGTTCCGG ATCCTCGGGG ACACCAAATA TGGCGATCTC
  181 GGCCTTTTCG TTTCTTGGAG CTGGGACATG TTTGCCATCG ATCCATCTAC CACCAGAACG
  241 GCCGTTAGAT CTGCTGCCAC CGTTGTTTCC ACCGAAGAAA CCACCGTTGC CGTAACCACC
  301 ACGACGGTTG TTGCTAAAGA AGCTGCCACC GCCACGGCCA CCGTTGTAGC CGCCGTTGTT
  361 GTTATTGTAG TTGCTACTGT TATTTCTGGC ACTTCTTGGT TTTCCTCTTA AGTGAGGAGG
  421 AACATAACCA TTCTCGTTGT TGTCGTTGAT GCTTAAATTT TGCACTTGTT CGCTCAGTTC
  481 AGCCATAATA TGAAATGCTT TTCTTGTTGT TCTTACGGAA TACCACTTGC CACCTATCAC
  541 CACAACTAAC TTTTTCCCGT TCCTCCATCT CTTTTATATT TTTTTTCTCG AGGGATCTTT
  601 GTGAAGGAAC CTTACTTCTG TGGTGTGACA TAATTGGACA AACTACCTAC AGAGATTTAA
  661 AGCTCTAAGG TAAATATAAA ATTTTTAAGT GTATAATGTG TTAAACTACT GATTCTAATT
  721 GTTTGTGTAT TTTAGATTCC AACCTATGGA ACTGATGAAT GGGAGCAGTG GTGGAATGCC
  781 TTTAATGAGG AAAACCTGTT TTGCTCAGAA GAAATGCCAT CTAGTGATGA TGAGGCTACT
  841 GCTGACTCTC AACATTCTAC TCCTCCAAAA AAGAAGAGAA AGGTAGAAGA CCCCAAAGGA
  901 CTTTCCTTCA GAATTTGCTA AGTTTTTTTG AGTCATGCTG TGTTTAGTAA TAGAACTCTT
  961 GCTTGCTTTG CTATTTACAC CACAAGGAAA AAGCTGCACT GCTATACAAG AAAATTATGG
 1021 GAAAAAATAT TCTGTAACCC TTTTATAAGT AGGCATACAG TTATATCATA CATACCTGTT
 1081 TTTTTCTACT CACACAGGCA TAGACTGTCT GCCTATATAC CTATGGCTCA AAAATTGTGT
1141 GGTATAACGT TTAAGGCCTT A
//
Primer: pAxCALNLF1
Sequence file: RDB15047_A7Enb.seq check
>D04276A5_A7En_1_pAxCALNLF1_C03_09_ABI24.ab1
    1 ACAAATTTAT CTTATCATGT CTGGATCTGA CATGGTAAGT AAGCTTGGGC TGCAGGTCGA
   61 GGGACCTAGA AGTTCCTATA CTTTCTAGAG AATAGGAACT TCATGTCGAC GCCACCATGG
  121 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG
  181 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA
  241 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG
  301 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC
  361 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA
  421 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA
  481 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC
  541 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA
  601 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC
  661 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
  721 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
  781 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTAAGATA
841 TCCTAGATCG AGTTAATTAA GGATTCCGCC CCCCCCCCCC TCTCCCTCCC
//
Primer: PRE_rev
Sequence file: RDB15047_A7Enc.seq check
>D04276A5_A7En_1_PRE_rev_E03_15_ABI24.ab1
    1 ACGGATCCCA TAGCGTAAAG GAGCAACATA GTTAAGAATA CCAGTCAATC TTTCACAAAT
   61 TTTGTAATCC AGAGGTTGAG CGGCCGAAGG GCGAATTCGC GGCCGCTTAC TTAGTTACCC
  121 GGGGAGCATG TCAAGGTCAA AATCGTCAAG AGCGTCAGCA GGCAGCATAT CAAGGTCAAA
  181 GTCGTCAAGG GCATCGGCTG GGAGCATGTC TAAGTCAAAA TCGTCAAGGG CGTCGGCCGG
  241 CCCGCCGCTT TCGCACTTTA GCTGTTTCTC CAGGCCACAT ATGATTAGTT CCAGGCCGAA
  301 AAGGAAGGCA GGTTCGGCTC CCTGATGGTC GAACAGCTCA ATTGCTTGTC TCAGAAGTGG
  361 GGGCATAGAA TCGGTGGTAG GTGTCTCTCT TTCCTCTTTT GCTACTTGAT GCTCCTGATC
  421 CTCCAATACG CAGCCCAGTG TAAAGTGGCC CACGGCGGAC AGAGCGTACA GTGCGTTCTC
  481 CAGGGAGAAG CCTTGCTGAC ACAGGAACGC GAGCTGATTT TCCAGGGTTT CGTACTGTTT
  541 CTCTGTTGGG CGGGTGCCGA GATGCACTTT AGCCCCGTCG CGATGTGAGA GGAGAGCACA
  601 GCGGAATGAC TTGGCGTTGT TCCGCAGAAA GTCTTGCCAT GACTCGCCTT CCAGGGGGCA
  661 GAAGTGGGTA TGATGCCTGT CCAGCATCTC GATTGCCAGG GCATCGAGCA GGGCCCGCTT
  721 GTTCTTCACG TGCCAGTACA GGGTAGGCTG CTCAACTCCC AGCTTTTGAG CGAGTTTCCT
  781 TGTCGTCAGG CCTTCGATAC CGACTTCATT GAGTAATTCC AGAGCAGAGT TTATGACTTT
  841 GCTCTTGTCC AGTCTAGACA TCGTGTTTTT CAAAGGAAAC CACGTCCCCG TGGTTTCGGG
  901 GGGCCTAGAC GTTTTTTTTA ACCTCGACTA AACACATGTA AAGCATGTGC ACCGAGGCCC
  961 CAGATCAGAT CCCATACATG GGGTACCTTC TGGCATCCTT CAGCCCCTTG TGATACGCTT
 1021 GAGGAGAGCC ATTTTACTCT TTCACACTAT CAACTCACAC GTGCACTGAG ATGTGCCGCT
1081 GCAGGTATCC TATACACGTG GCTTTGACGC AGAAGGCCAA CC
//
Primer: SV40pro_F_V2
Sequence file: RDB15047_A7End.seq check
>D04276A5_A7En_1_SV40pro_F_V2_F03_18_ABI24.ab1
    1 GGACCGCCAT TTTTGGGAGG CTAGGCTTTT GCAAGATCGA TCAAGAGACA GGATGAGGAT
   61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
  601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  781 ACCGCTTCCT CGTGCTTTAC GGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
  841 CGCCTTCTTG ACGAGTTCTT CTGGAGCGGG ACTTCTGGGG TTCGAAATGA CGACAGCGAC
  901 GCCCATCTGC ATCACGGAGA TTCGATTCCA CGCGCCTTCT TATGAAGTGG CTTCGATCGG
  961 TTCGGGAACG CCGGCCTGGA TGATCTCAGC GCGGGACTCC TATGCTGAAT TCTTCGCCTA
1021 CTAGGGAAGC TAACTGAACT CGA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 20775045.

2020.12.14

GNP_filter3_RDBDEP_html_201104.pl