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pK036.TRE-Flpe-WPRE (Supernova) (#RDB15046)

Flippase (Flpe)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Flpe recombinase.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Luo, W., Sci. Rep. 6: 35747 (2016). PMID 27775045. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name pK036
Clone info. Flippase (Flpe)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Flpe recombinase. This Flpe expression vector will be used with pK037 (RDB15048) and pk068 (RDB15047). Please refer Fig.6 of Luo, W. et al. Sci. Rep., 6: 35747, 2016.
Comment Expression was confirmed by the depositor with immunohistochemistry and FACS.
Vector backbone pTRE-Tight (plasmid)
Selectable markers Amp^r (E. coli).
Growth conditions LB+Amp, 37oC
Depositor|Developer Iwasato, Takuji |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Luo, W. et al. Sci. Rep., 6:35747, 2016)。

Catalog # Resource name Shipping form Fee
RDB15046 pK036.TRE-Flpe-WPRE (Supernova) DNA solution

How to cite this biological resource

Materials & Methods section:

The pK036.TRE-Flpe-WPRE (Supernova) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15046).

Reference section:

Luo, W., Mizuno, H., Iwata, R., Nakazawa, S., Yasuda, K., Itohara, S., Iwasato, T., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045. [PubMed] [Article] [RRC of NBRP]