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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pK036.TRE-Flpe-WPRE (Supernova)

Flippase (Flpe)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Flpe recombinase.

Catalog number RDB15046
Resource name pK036.TRE-Flpe-WPRE (Supernova)
Alternative name pK036
Clone info. Flippase (Flpe)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Flpe recombinase. This Flpe expression vector will be used with pK037 (RDB15048) and pk068 (RDB15047). Please refer Fig.6 of Luo, W. et al. Sci. Rep., 6: 35747, 2016.
Expression was confirmed by the depositor with immunohistochemistry and FACS.
Vector backbone pTRE-Tight (plasmid)
Selectable markers Amp^r (E. coli).
Depositor|Developer Iwasato, Takuji |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).
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RDB15046 pK036.TRE-Flpe-WPRE (Supernova) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 20775045.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15046_A7Enp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Amp_R
Sequence file: RDB15046_A7Ena.seq check
>D04276A4_A7En_2_Amp_R_G02_20_ABI24.ab1
    1 TTCGTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
  181 AGGCCCTTTC GTCTTCACTC GAGTTTACTC CCTATCAGTG ATAGAGAACG TATGTCGAGT
  241 TTACTCCCTA TCAGTGATAG AGAACGATGT CGAGTTTACT CCCTATCAGT GATAGAGAAC
  301 GTATGTCGAG TTTACTCCCT ATCAGTGATA GAGAACGTAT GTCGAGTTTA CTCCCTATCA
  361 GTGATAGAGA ACGTATGTCG AGTTTATCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT
  421 ACTCCCTATC AGTGATAGAG AACGTATGTC GAGGTAGGCG TGTACGGTGG GAGGCCTATA
  481 TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG AATTCGAGCT CGGTACCCGG
  541 GGATCCTTGT GCTGTCTCAT CATTTTGGCA AAGAATTACC CGCCGCCACC ATGGCTCCCA
  601 AGAAGAAGAG GAAGGTGATG AGTCAATTTG ATATATTATG TAAAACACCA CCTAAGGTCC
  661 TGGTTCGTCA GTTTGTGGAA AGGTTTGAAA GACCTTCAGG GGAAAAAATA GCATCATGTG
  721 CTGCTGAACT AACCTATTTA TGTTGGATGA TTACTCATAA CGGAACAGCA ATCAAGAGAG
  781 CCACATTCAT GAGCTATAAT ACTATCATAA GCAATTCGCT GAGTTTCGAT ATTGTCAACA
  841 AATCACTCCA GTTTAAATAC AAGACGCAAA AAGCAACAAT TCTGGAAGCC TCATTAAAGA
  901 AATTAATTCC TGCTTGGGAA ATTTACAATT ATTCCCTTAC AATGGACAAA AACATCAATC
  961 TGATATCACT GATATTTGTA AGTAGTTTGC AATTACAGTT CGAATCATCG GGAGAAGCAG
 1021 ATATGGAAAT AGCCACAGTA AAAAAATTGC TAAGCACTCT AGTGAGGGTG AAAGCATCTG
1081 GGAGATCACT GAGAAACTAA TTCGTGAGTA TACTCGAGAT TACCAAAAGA AGAACCT
//
Primer: ME-1290RV
Sequence file: RDB15046_A7Enb2.seq check
>D04276A4_A7En_2_ME-1290RV_D01_10_ABI24.ab1
    1 GTGGTGCAAT TTCATTTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG
   61 CAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT CCTCTAGAGA TATCGTCGAC
  121 AAGCTTATCG ATGCGGCCGC TGCGGGGAGG CGGCCCAAAG GGAGATCCGA CTCGTCTGAG
  181 GGCGAAGGCG AAGACGCGGA AGAGGCCGCA GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT
  241 CCGCTGGATT GAGGGCCGAA GGGACGTAGC AGAAGGACGT CCCGCGCAGA ATCCAGGTGG
  301 CAACATAGGC GAGCAGCCAA GGAAAGGACG ATGATTTCCC CGACAACACC ACGGAATTGT
  361 CAGTGCCCAA CAGCCGAGCC CCTGTCCAGC AGCGGGCAAG GCAGGCGGCG ATGAGTTCCG
  421 CCGTGGCAAT AGGGAGGGGG AAAGCGAAAG TTCCGGAAAG GAGCTGACAG GTGGTGGCAA
  481 TGCCCCAACC AGTGGGGGTT GCGTCAGCAA ACACAGTGCA CACCACGCCA CGTTGCCTGA
  541 CAACGGGCCA CAACTCCTCA TAAAGAGACA GCAACCAGGA TTTATACAAG GAGGAGAAAA
  601 TGAAAGCCAT ACGGGAAGCA ATAGCATGAT ACAAAGGCAT TAAAGCAGCG TATCCACATA
  661 GCGTAAAAGG AGCAACATAG TTAAGAATAC CAGTCAATCT TTCACAAATT TTGTAATCCA
  721 GAGGTTGAGC GGCCGAAGGG CGAATTCGCG GCCGCGGGCG GAATTCTTAA TTAACTCGAC
  781 TCTAGATCAT TATATGCGTC TATTTATGTA GGATGAAAGG TAGTCTAGTA CCTCCTGTGA
  841 TATTATCCCA TTCCATGCGG GGTATCGTAT GCTTCCTTCA GCACTACCCT TTAGCTGTTC
  901 TATATGCTGC CACTCCTCAA TTGGATTAGT CTCATCCTTC AATGCTATCA TTTCCTTTGA
  961 TATTGGATCA TATGCATAGT ACCGAGAAAC TAGTGCGAAG TAGTGATCAG GTATTGCTGT
 1021 TATCTGATGA GTATACGTTG TCCTGCCACG GCAGAAGGCA CGCTTATCGG CTCCATTCCC
 1081 ACAACATTTA GTTCAACTTC GTAGCCTCCA TGACGAAATG AAGTCATCCA AATGTTCTTT
1141 CCAAAT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 20775045.

2020.12.14

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