Please review the QC test results indicated by icon below as well as clone information before placing your order.

pK036.TRE-Flpe-WPRE (Supernova)

Flippase (Flpe)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Flpe recombinase.

Sequence (full)	RDB15046zzk02.seq provided by the depositor

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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Luo, W. et al. Sci. Rep., 6:35747, 2016)。
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15046_B2Cfp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Amp_R (Pr0008) Region: AmpR pro, tight TRE pro, insert 5' (FLPe 5') Sequence file: RDB15046_B2Cfc.seq
>15046_15046_B2Cf_Amp_R_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNATTTATC NGGGTTATTG TCTCNTGAGC GGATACATAT    61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC   121 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA   181 CGAGGCCCTT TCGTCTTCAC TCGAGTTTAC TCCCTATCAG TGATAGAGAA CGTATGTCGA   241 GTTTACTCCC TATCAGTGAT AGAGAACGAT GTCGAGTTTA CTCCCTATCA GTGATAGAGA   301 ACGTATGTCG AGTTTACTCC CTATCAGTGA TAGAGAACGT ATGTCGAGTT TACTCCCTAT   361 CAGTGATAGA GAACGTATGT CGAGTTTATC CCTATCAGTG ATAGAGAACG TATGTCGAGT   421 TTACTCCCTA TCAGTGATAG AGAACGTATG TCGAGGTAGG CGTGTACGGT GGGAGGCCTA   481 TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGAATTCGAG CTCGGTACCC   541 GGGGATCCTT GTGCTGTCTC ATCATTTTGG CAAAGAATTA CCCGCCGCCA CCATGGCTCC   601 CAAGAAGAAG ANGAAGGTGA TGAGTCANTT TGATATATTA TGTNAAACAC CACCTAANGN   661 CCTGGTTCGT CNGTTTGNNG NAAAGGNTTG NNNANNTTCN NNNNAAANNA GNATCNTGNG 721 CTGCTGNNCT NNCCNNNTTN NNNNNNGNNN NNNNNNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert 3' (WPRE) Sequence file: RDB15046_B2Cfd.seq
>15046_15046_B2Cf_SV40polyA_R2_H01_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN AACAACAACN ATTGCATTCN TTTTATGTTT    61 CAGGTTCNGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGNN   121 NNNNCTGATT ATGATCCTCT AGAGATATCG TCGACAAGCT TATCGATGCG GCCGCTGCGG   181 GGAGGCGGCC CAAAGGGAGA TCCGACTCGT CTGAGGGCGA AGGCGAAGAC GCGGAAGAGG   241 CCGCAGAGCC GGCAGCAGGC CGCGGGAAGG AAGGTCCGCT GGATTGAGGG CCGAAGGGAC   301 GTAGCAGAAG GACGTCCCGC GCAGAATCCA GGTGGCAACA TAGGCGAGCA GCCAAGGAAA   361 GGACGATGAT TTCCCCGACA ACACCACGGA ATTGTCAGTG CCCAACAGCC GAGCCCCTGT   421 CCAGCAGCGG GCAAGGCAGG CGGCGATGAG TTCCGCCGTG GCAATAGGGA GGGGGAAAGC   481 GAAAGTTCCG GAAAGGAGCT GACAGGTGGT GGCAATGCCC CAACNAGTGG GGGTTGCGTC   541 AGCAAACACA GTGCACACCA CGCCACGTTG CCTGACNACG GGCCANNACT CCTCATAANN   601 ANACAGCAAC CAGGANTTAT ACNAGGAGGA GANNATGAAA GCCNNNNNGG AAGCAATAGC   661 ATGATACNNA NGCANNAAAG NNNCGNANNC NNNTAGCGNA AAAGGAGCNN CATANNNNAN 721 ANNNNCNNNC NANNCTTTNN NNNANNTTTG NNNNNCNNNN NGNNNNNNNN //
Primer: tetpro-B_R (Pr0732) Region: tight TRE pro, insert 5' (FLPe 5') Sequence file: RDB15046_B2Cfe.seq
>15046_15046_B2Cf_tetpro-B_R_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNG NNNNNNATGT CGAGTTTACT CCCTATCNGT GATAGAGAAC    61 GATGTCGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG TCGAGTTTAC TCCCTATCAG   121 TGATAGAGAA CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT   181 ATCCCTATCA GTGATAGAGA ACGTATGTCG AGTTTACTCC CTATCAGTGA TAGAGAACGT   241 ATGTCGAGGT AGGCGTGTAC GGTGGGAGGC CTATATAAGC AGAGCTCGTT TAGTGAACCG   301 TCAGATCGCC TGGAGAATTC GAGCTCGGTA CCCGGGGATC CTTGTGCTGT CTCATCATTT   361 TGGCAAAGAA TTACCCGCCG CCACCATGGC TCCCAAGAAG AAGAGGAAGG TGATGAGTCA   421 ATTTGATATA TTATGTAAAA CACCACCTAA GGTCCTGGTT CGTCAGTTTG TGGAAAGGTT   481 TGAAAGACCT TCAGGGGAAA AAATAGCATC ATGTGCTGCT GAACTAACCT ATTTATGTTG   541 GATGATTACT CATAACGGAA CAGCAATCAA GAGAGCCACA TTCATGAGCT ATAATACTAT   601 CATAAGCAAT TCGCTGAGTT TCGATATTGT CAACAAATCA CTCCAGTTTA AATACAAGAC   661 GCAAAAAGCA ACAATTCTGG AAGCCTCATT AAAGAAATTA ATTCCTGCTT GGGAATTTAC   721 AATTATTCCT TACAATGGAC AAAAACATCA ATCTGATATC ACTGATATTG TAAGTAGTTT   781 GCAATTACAG TTCGAATCAT CGGAAGAAGC AGATAANGGA AATAGCCACA GTAAAAAAAT   841 GCTTAAAGCA CTTCTAAGTG AGGGTGAAAG CATCTGGGAN ANCACTGAGA AAATACTAAA   901 TTCGTTTGAG TATACNTCGA GANTTACNAA AACNAAANCT TTATANNNAT TCCNCTTNCT   961 ANCNACTTTC ATCNNNTGTG GAAGANTCNN CGANNTTNNN AACNNNGNTN NNAAATCNTT 1021 TAANTTANNN CNAAATNNGT NNNNGGGNNT NNNNNTCNNN NNNTNNNNNN NNNN //
Primer: PRE_rev (Pr0250) Region: insert 3' (WPRE, FLPe 3') Sequence file: RDB15046_B2Cff.seq
>15046_15046_B2Cf_PRE_rev_H01_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NANGGAGCAA CATAGTTAAG AATACCNNTC AATCTTTNAC    61 AAATTTTGTA ATCCAGAGGT TGAGCGGCCG AAGGGCGAAT TCGCGGCCGC GGGCGGAATT   121 CTTAATTAAC TCGACTCTAG ATCATTATAT GCGTCTATTT ATGTAGGATG AAAGGTAGTC   181 TAGTACCTCC TGTGATATTA TCCCATTCCA TGCGGGGTAT CGTATGCTTC CTTCAGCACT   241 ACCCTTTAGC TGTTCTATAT GCTGCCACTC CTCAATTGGA TTAGTCTCAT CCTTCAATGC   301 TATCATTTCC TTTGATATTG GATCATATGC ATAGTACCGA GAAACTAGTG CGAAGTAGTG   361 ATCAGGTATT GCTGTTATCT GATGAGTATA CGTTGTCCTG GCCACGGCAG AAGCACGCTT   421 ATCGCTCCAA TTTCCCACAA CATTAGTCAA CTCCGTTAGG CCCTTCATTG ACAGAAATGA   481 GGTCATCAAA TGTCTTCCAA TGTGAGATTT TGGGCCATTC TTTATAGCAA AGATTGGATA   541 AGGCGCATTT TTCTTCNAAG CCTTGTTGTA CGATCTGACT AAGTTATCTT TTAATAATTG   601 GTATTCCTGT TTGTTGCTTG AAGAATTGCC GGTCCTATTT ACTCGTTTTA NGANTGGNTC   661 NNAATTCCTC NAAAATTCNT CCNAATATAN NNNGGNTCGA TNCTACCCCN TGNNCTNANN 721 AAGTANATNN NNNNACNAAN NNNNNNNNTN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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