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pK036.TRE-Flpe-WPRE (Supernova)

Flippase (Flpe)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Flpe recombinase.

Catalog number RDB15046
Resource name pK036.TRE-Flpe-WPRE (Supernova)
Alternative name pK036
Clone info. Flippase (Flpe)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Flpe recombinase. This Flpe expression vector will be used with pK037 (RDB15048) and pk068 (RDB15047). Please refer Fig.6 of Luo, W. et al. Sci. Rep., 6: 35747, 2016.
Comment Expression was confirmed by the depositor with immunohistochemistry and FACS.
Vector backbone pTRE-Tight (plasmid)
Selectable markers Amp^r (E. coli).
Depositor|Developer Iwasato, Takuji |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Luo, W. et al. Sci. Rep., 6:35747, 2016)。
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            RDB15046 pK036.TRE-Flpe-WPRE (Supernova) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB15046zzk02.seq provided by the depositor

            Original reference

            original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15046_B2Cfp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: Amp_R
            Region: AmpR pro, tight TRE pro, insert 5' (FLPe 5')
            Sequence file: RDB15046_B2Cfc.seq check
            >15046_15046_B2Cf_Amp_R_G01_07_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNATTTATC NGGGTTATTG TCTCNTGAGC GGATACATAT
               61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
              121 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA
              181 CGAGGCCCTT TCGTCTTCAC TCGAGTTTAC TCCCTATCAG TGATAGAGAA CGTATGTCGA
              241 GTTTACTCCC TATCAGTGAT AGAGAACGAT GTCGAGTTTA CTCCCTATCA GTGATAGAGA
              301 ACGTATGTCG AGTTTACTCC CTATCAGTGA TAGAGAACGT ATGTCGAGTT TACTCCCTAT
              361 CAGTGATAGA GAACGTATGT CGAGTTTATC CCTATCAGTG ATAGAGAACG TATGTCGAGT
              421 TTACTCCCTA TCAGTGATAG AGAACGTATG TCGAGGTAGG CGTGTACGGT GGGAGGCCTA
              481 TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGAATTCGAG CTCGGTACCC
              541 GGGGATCCTT GTGCTGTCTC ATCATTTTGG CAAAGAATTA CCCGCCGCCA CCATGGCTCC
              601 CAAGAAGAAG ANGAAGGTGA TGAGTCANTT TGATATATTA TGTNAAACAC CACCTAANGN
              661 CCTGGTTCGT CNGTTTGNNG NAAAGGNTTG NNNANNTTCN NNNNAAANNA GNATCNTGNG
            721 CTGCTGNNCT NNCCNNNTTN NNNNNNGNNN NNNNNNN
            //
            Primer: SV40polyA_R2
            Region: insert 3' (WPRE)
            Sequence file: RDB15046_B2Cfd.seq check
            >15046_15046_B2Cf_SV40polyA_R2_H01_08_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN AACAACAACN ATTGCATTCN TTTTATGTTT
               61 CAGGTTCNGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGNN
              121 NNNNCTGATT ATGATCCTCT AGAGATATCG TCGACAAGCT TATCGATGCG GCCGCTGCGG
              181 GGAGGCGGCC CAAAGGGAGA TCCGACTCGT CTGAGGGCGA AGGCGAAGAC GCGGAAGAGG
              241 CCGCAGAGCC GGCAGCAGGC CGCGGGAAGG AAGGTCCGCT GGATTGAGGG CCGAAGGGAC
              301 GTAGCAGAAG GACGTCCCGC GCAGAATCCA GGTGGCAACA TAGGCGAGCA GCCAAGGAAA
              361 GGACGATGAT TTCCCCGACA ACACCACGGA ATTGTCAGTG CCCAACAGCC GAGCCCCTGT
              421 CCAGCAGCGG GCAAGGCAGG CGGCGATGAG TTCCGCCGTG GCAATAGGGA GGGGGAAAGC
              481 GAAAGTTCCG GAAAGGAGCT GACAGGTGGT GGCAATGCCC CAACNAGTGG GGGTTGCGTC
              541 AGCAAACACA GTGCACACCA CGCCACGTTG CCTGACNACG GGCCANNACT CCTCATAANN
              601 ANACAGCAAC CAGGANTTAT ACNAGGAGGA GANNATGAAA GCCNNNNNGG AAGCAATAGC
              661 ATGATACNNA NGCANNAAAG NNNCGNANNC NNNTAGCGNA AAAGGAGCNN CATANNNNAN
            721 ANNNNCNNNC NANNCTTTNN NNNANNTTTG NNNNNCNNNN NGNNNNNNNN
            //
            Primer: tetpro-B_R
            Region: tight TRE pro, insert 5' (FLPe 5')
            Sequence file: RDB15046_B2Cfe.seq check
            >15046_15046_B2Cf_tetpro-B_R_D01_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNG NNNNNNATGT CGAGTTTACT CCCTATCNGT GATAGAGAAC
               61 GATGTCGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG TCGAGTTTAC TCCCTATCAG
              121 TGATAGAGAA CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT
              181 ATCCCTATCA GTGATAGAGA ACGTATGTCG AGTTTACTCC CTATCAGTGA TAGAGAACGT
              241 ATGTCGAGGT AGGCGTGTAC GGTGGGAGGC CTATATAAGC AGAGCTCGTT TAGTGAACCG
              301 TCAGATCGCC TGGAGAATTC GAGCTCGGTA CCCGGGGATC CTTGTGCTGT CTCATCATTT
              361 TGGCAAAGAA TTACCCGCCG CCACCATGGC TCCCAAGAAG AAGAGGAAGG TGATGAGTCA
              421 ATTTGATATA TTATGTAAAA CACCACCTAA GGTCCTGGTT CGTCAGTTTG TGGAAAGGTT
              481 TGAAAGACCT TCAGGGGAAA AAATAGCATC ATGTGCTGCT GAACTAACCT ATTTATGTTG
              541 GATGATTACT CATAACGGAA CAGCAATCAA GAGAGCCACA TTCATGAGCT ATAATACTAT
              601 CATAAGCAAT TCGCTGAGTT TCGATATTGT CAACAAATCA CTCCAGTTTA AATACAAGAC
              661 GCAAAAAGCA ACAATTCTGG AAGCCTCATT AAAGAAATTA ATTCCTGCTT GGGAATTTAC
              721 AATTATTCCT TACAATGGAC AAAAACATCA ATCTGATATC ACTGATATTG TAAGTAGTTT
              781 GCAATTACAG TTCGAATCAT CGGAAGAAGC AGATAANGGA AATAGCCACA GTAAAAAAAT
              841 GCTTAAAGCA CTTCTAAGTG AGGGTGAAAG CATCTGGGAN ANCACTGAGA AAATACTAAA
              901 TTCGTTTGAG TATACNTCGA GANTTACNAA AACNAAANCT TTATANNNAT TCCNCTTNCT
              961 ANCNACTTTC ATCNNNTGTG GAAGANTCNN CGANNTTNNN AACNNNGNTN NNAAATCNTT
            1021 TAANTTANNN CNAAATNNGT NNNNGGGNNT NNNNNTCNNN NNNTNNNNNN NNNN
            //
            Primer: PRE_rev
            Region: insert 3' (WPRE, FLPe 3')
            Sequence file: RDB15046_B2Cff.seq check
            >15046_15046_B2Cf_PRE_rev_H01_08_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NANGGAGCAA CATAGTTAAG AATACCNNTC AATCTTTNAC
               61 AAATTTTGTA ATCCAGAGGT TGAGCGGCCG AAGGGCGAAT TCGCGGCCGC GGGCGGAATT
              121 CTTAATTAAC TCGACTCTAG ATCATTATAT GCGTCTATTT ATGTAGGATG AAAGGTAGTC
              181 TAGTACCTCC TGTGATATTA TCCCATTCCA TGCGGGGTAT CGTATGCTTC CTTCAGCACT
              241 ACCCTTTAGC TGTTCTATAT GCTGCCACTC CTCAATTGGA TTAGTCTCAT CCTTCAATGC
              301 TATCATTTCC TTTGATATTG GATCATATGC ATAGTACCGA GAAACTAGTG CGAAGTAGTG
              361 ATCAGGTATT GCTGTTATCT GATGAGTATA CGTTGTCCTG GCCACGGCAG AAGCACGCTT
              421 ATCGCTCCAA TTTCCCACAA CATTAGTCAA CTCCGTTAGG CCCTTCATTG ACAGAAATGA
              481 GGTCATCAAA TGTCTTCCAA TGTGAGATTT TGGGCCATTC TTTATAGCAA AGATTGGATA
              541 AGGCGCATTT TTCTTCNAAG CCTTGTTGTA CGATCTGACT AAGTTATCTT TTAATAATTG
              601 GTATTCCTGT TTGTTGCTTG AAGAATTGCC GGTCCTATTT ACTCGTTTTA NGANTGGNTC
              661 NNAATTCCTC NAAAATTCNT CCNAATATAN NNNGGNTCGA TNCTACCCCN TGNNCTNANN
            721 AAGTANATNN NNNNACNAAN NNNNNNNNTN NNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl