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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

Cre-based Supernova series. Expression vector of loxP-stop-loxP-EGFP under the CAG promoter.

Catalog number RDB15045
Resource name pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)
Alternative name pK038
Clone info. Cre-based Supernova series. Expression vector of loxP-stop-loxP-EGFP under the CAG promoter. Cre-mediated recombination, a loxP-stop-loxP is excised and the downstream EGFP is expressed. The structure of the construct, see Fig.7 of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Comment Expression was confirmed by the depositor with immunohistochemistry and FACS.
Vector backbone pK029 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Depositor|Developer Iwasato, Takuji |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Mizuno, H. et al. Neuron, 82: 365-379, 2014).
            Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Mizuno, H. et al. Neuron, 82: 365-379, 2014)。
            付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15045 pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB15045zzk02.seq provided by the depositor

            Original reference

            original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15045_A7Enp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxCALNLF1
            Region: loxP,insert 5'
            Sequence file: RDB15045_A7Ene.seq check
            >D04276A3_A7En_1_pAxCALNLF1_C01_07_ABI24.ab1
                1 NNNNNNNNNN NNNTNNTGTC TGGNNCTGAN NTGGTAAGTA AGCTTGGGCT GCAGGTCGAG
               61 GGANCTAATA ACTTCGTATA GCATACATTA TACGAAGTTA TATGTCGACG CCACCATGGT
              121 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA
              181 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA
              241 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT
              301 GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA
              361 CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA
              421 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA
              481 CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT
              541 GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT
              601 CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA
              661 CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT
              721 GAGCACCCAG TCCGCCCTGA GCAAAGANCC CAACGAGAAG CGCGATCACA TGGTCCTGCT
              781 GGAGTTCGTG ACCGCCGCCG GNNTCACTCT CGGCATGGAC GANCTGTACA AGTAAGANAT
              841 CCTAGATCGA GTTNATTNAN NNNTCNNCCC CCCCCCCCCN CNCCCNCCCC CCCCCCCNAA
              901 CGTTACNGGN CNAAGCNNCT TGGAANNNNN NCNGNGNGCG TTNGNCNNNN TNTNATTTTN
            961 NNNCNNANNG CCGTNTTTNG NNNNNGNGNN NNCNNNAANN NNNNNNNN
            //
            Primer: mVenus-M_F
            Region: insert 3',IRES
            Sequence file: RDB15045_A7Enf.seq check
            >15045_15045_B2CgF_mVenus-M_F_D01_04_ABI08.ab1
                1 NNNNNNNNNN NNGGCNACGG CCCCGNGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG
               61 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
              121 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAGATAT CCTAGATCGA
              181 GTTAATTAAG GATTCCGCCC CCCCCCCTCT CCCTCCCCCC CCCCNNNNNN TACTGNNNNA
              241 NNCCGCTTGG AATAAGGCCG GTGTGCGTTT GNCTNNNNGT TATTTTNNNN NNTATTGCCN
            301 NNNTTTNNNN NNGNNNNGGC CCGNAANNCT GNNCCTGTCT TCTTGNCNAN NNNNNNNNN
            //
            Primer: pAxCA_F1
            Region: loxP
            Sequence file: RDB15045_A7Eng.seq check
            >D04276A3_A7En_1_pAxCA_F1_A02_02_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNGCCTT CTTCTTTTTC CNACNGCTCC TGGGCAACGT
               61 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC TAATAACTTC GTATAGCATA
              121 CATTATACGA AGTTATATTA AGGGTTCCGG ATCCTCGGGG ACACCAAATA TGGCGATCTC
              181 GGCCTTTTCG TTTCTTGGAG CTGGGACATG TTTGCCATCG ATCCATCTAC CACCAGAACG
              241 GCCGTTAGAT CTGCTGCCAC CGTTGTTTCC ACCGAAGAAA CCACCGTTGC CGTAACCACC
              301 ACGACGGTTG TTGCTAAAGA AGCTGCCACC GCCACGGCCA CCGTTGTAGC CGCCGTTGTT
              361 GTTATTGTAG TTGCTACTGT TATTTCTGGC ACTTCTTGGT TTTCCTCTTA AGTGAGGAGG
              421 AACATAACCA TTCTCGTTGT TGTCGTTGAT GCTTAAATTT TGCACTTGTT CGCTCAGTTC
              481 AGCCATAATA TGAAATGCTT TTCTTGTTGT TCTTACGGAA TACCACTTGC CACCTATCAC
              541 CACAACTAAC TTTTTCCCGT TCCTCCATCT CTTTTATATT TTTTTTCTCG AGGGATCTTT
              601 GTGAAGGAAC CTTACTTCTG TGGTGTGACA TAATTGGACA AACTACCTAC AGAGATTTAA
              661 AGCTCTAANG TAAATATAAN ATTTTTAAGT GTATAATGTG TTAAACTACT GATTCTAATT
              721 GTTTGTGTAT TTTANATTCC AACCTATGGA ACTGATGAAT GGGANCANTG GTGGAATGNC
              781 TTTNATGANG AAAACCNGNT TNGCTNNNAN NAAANGNCNT CTANNGATGA NGNNNNTACN
              841 GNNNANNNNC NNNNTTCNAC TNNNNNNAAA AANANNANAA NNNNANANNA NCCNNANNNA
            901 NNTTNNNTNN NNNNNNNN
            //
            Primer: PRE_rev
            Region: WPRE,tTA Advanceed
            Sequence file: RDB15045_A7Enh.seq check
            >D04276A3_A7En_1_PRE_rev_E02_14_ABI24.ab1
                1 NNNNNNNNNN NNNCGNNNNG GANCNNCATA GTTAAGAATA CCAGTCAATC TTTCNCAAAT
               61 TTTGTAATCC AGAGGTTGAG CGGCCGAAGG GCGAATTCGC GGCCGCTTAC TTAGTTACCC
              121 GGGGAGCATG TCAAGGTCAA AATCGTCAAG AGCGTCAGCA GGCAGCATAT CAAGGTCAAA
              181 GTCGTCAAGG GCATCGGCTG GGAGCATGTC TAAGTCAAAA TCGTCAAGGG CGTCGGCCGG
              241 CCCGCCGCTT TCGCACTTTA GCTGTTTCTC CAGGCCACAT ATGATTAGTT CCAGGCCGAA
              301 AAGGAAGGCA GGTTCGGCTC CCTGATGGTC GAACAGCTCA ATTGCTTGTC TCAGAAGTGG
              361 GGGCATAGAA TCGGTGGTAG GTGTCTCTCT TTCCTCTTTT GCTACTTGAT GCTCCTGATC
              421 CTCCAATACG CAGCCCAGTG TAAAGTGGCC CACGGCGGAC AGAGCGTACA GTGCGTTCTC
              481 CAGGGAGAAG CCTTGCTGAC ACAGGAACGC GAGCTGATTT TCCAGGGTTT CGTACTGTTT
              541 CTCTGTTGGG CGGGTGCCGA GATGCACTTT AGCCCCGTCG CGATGTGAGA GGAGAGCACA
              601 GCGGAATGAC TTGGCGTTGT TCCGCAGAAA GTCTTGCCAT GACTCGCCTT CCAGGGGGCA
              661 GAAGTGGGTA TGATGCCTGT CCAGCATCTC GATTGCCAGG GCATCGAGCA NGGCCCGCTT
              721 GTTCTTCACG TGCCAGTACA GGGTANGCTG CTCNACTCCC ANCTTTTGAN CGAGTTTCCT
              781 TGTCGTCAGG NCNTCNATAC CGACTTCNTT GANTAATTCC NGANCANANT TTATGANTTN
              841 GCTCTTGNCC AGNCNANACA TCNNGTTTTT CNAANGAAAN NNCNNCCCNN NNNNNCGGGG
            901 GNNNTANNNN TTTTTTNNNN NNNNNN
            //
            Primer: SV40pro_F_V2
            Region: NeoR_KanR
            Sequence file: RDB15045_A7Eni.seq check
            >D04276A3_A7En_1_SV40pro_F_V2_F02_17_ABI24.ab1
                1 NNNNNNNNNN NNNNNCNNNG CTTTTGNAAG NNCGATCNAG AGACAGGNTG AGGNNCGTTT
               61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
              121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
              181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
              241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
              301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
              361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
              421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
              481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
              541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC
              601 GACGGCGAGG NTCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
              661 ANTGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA NCGCTATCAN
              721 GACATAGCGT TGGCTACCCG TGATATTGCT GAANANCTTG GCGGCGAATG GGCTGACCGC
              781 TTCCTCGNGC TTTACGGTAT CGCCGCTCCC NANNCGCAGC GCATCGCCTT CTATCGCCNT
              841 CNTGANNANT TCNTCTGANC GGGNNTNTGG GGTTCNAAAT GANNNANCNA GCGNCGCCNN
            901 NNCNNNNNNT CNCNANNNTN CNATTNNNNN NNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl