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pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

Cre-based Supernova series. Expression vector of loxP-stop-loxP-EGFP under the CAG promoter.

Catalog number RDB15045
Resource name pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)
Alternative name pK038
Clone info. Cre-based Supernova series. Expression vector of loxP-stop-loxP-EGFP under the CAG promoter. Cre-mediated recombination, a loxP-stop-loxP is excised and the downstream EGFP is expressed. The structure of the construct, see Fig.7 of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Expression was confirmed by the depositor with immunohistochemistry and FACS.
Vector backbone pK029 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Depositor|Developer Iwasato, Takuji |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Mizuno, H. et al. Neuron, 82: 365-379, 2014).
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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Schedule A (GFP-A) [Link]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15045 pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Mizuno, H., NMDAR-regulated dynamics of layer 4 neuronal dendrites during thalamocortical reorganization in neonates. Neuron 82 (2): 365-379 (2014). PMID 24685175.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15045_A7Enp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB15045_A7Ena.seq check
>D04276A3_A7En_1_pAxCA_F1_A02_02_ABI24.ab1
    1 CGAACCAATA CCGCTACATG TTCATGCCTT CTTCTTTTTC CTACAGCTCC TGGGCAACGT
   61 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC TAATAACTTC GTATAGCATA
  121 CATTATACGA AGTTATATTA AGGGTTCCGG ATCCTCGGGG ACACCAAATA TGGCGATCTC
  181 GGCCTTTTCG TTTCTTGGAG CTGGGACATG TTTGCCATCG ATCCATCTAC CACCAGAACG
  241 GCCGTTAGAT CTGCTGCCAC CGTTGTTTCC ACCGAAGAAA CCACCGTTGC CGTAACCACC
  301 ACGACGGTTG TTGCTAAAGA AGCTGCCACC GCCACGGCCA CCGTTGTAGC CGCCGTTGTT
  361 GTTATTGTAG TTGCTACTGT TATTTCTGGC ACTTCTTGGT TTTCCTCTTA AGTGAGGAGG
  421 AACATAACCA TTCTCGTTGT TGTCGTTGAT GCTTAAATTT TGCACTTGTT CGCTCAGTTC
  481 AGCCATAATA TGAAATGCTT TTCTTGTTGT TCTTACGGAA TACCACTTGC CACCTATCAC
  541 CACAACTAAC TTTTTCCCGT TCCTCCATCT CTTTTATATT TTTTTTCTCG AGGGATCTTT
  601 GTGAAGGAAC CTTACTTCTG TGGTGTGACA TAATTGGACA AACTACCTAC AGAGATTTAA
  661 AGCTCTAAGG TAAATATAAA ATTTTTAAGT GTATAATGTG TTAAACTACT GATTCTAATT
  721 GTTTGTGTAT TTTAGATTCC AACCTATGGA ACTGATGAAT GGGAGCAGTG GTGGAATGCC
  781 TTTAATGAGG AAAACCTGTT TTGCTCAGAA GAAATGCCAT CTAGTGATGA TGAGGCTACT
  841 GCTGACTCTC AACATTCTAC TCCTCCAAAA AAGAAGAGAA AGGTAGAAGA CCCCAAAGGA
  901 CTTTCCTTTC AGAATTGCTA AGTTTTTTGA GTCATGCTGT GTTTAGTAAT AGAACTCTTG
  961 CTTTGCTTTG CTATTTACAC ACAAAGAAAA AGCTGCACTG CCTATACAAG AAATATGAAA
 1021 AATATTCCTG TACTTTATAG TAAGCATAAC AGTTATATCC ATAACCATAA CCGTTATTCT
1081 AGCTCCACAC AGGCATAGAG TCTGGCTATT TAATAAACT
//
Primer: pAxCALNLF1
Sequence file: RDB15045_A7Enb.seq check
>D04276A3_A7En_1_pAxCALNLF1_C02_08_ABI24.ab1
    1 TTTAATCTAT CTTATCATGT CTGGATCTGA CATGGTAAGT AAGCTTGGGC TGCAGGTCGA
   61 GGGACCTAAT AACTTCGTAT AGCATACATT ATACGAAGTT ATATGTCGAC GCCACCATGG
  121 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG
  181 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA
  241 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG
  301 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC
  361 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA
  421 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA
  481 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC
  541 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA
  601 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC
  661 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
  721 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
  781 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTAAGATA
841 TCCTAGATCG AGTTAATTAA AGGATTCCGC CCCCCCCCCC CTCTCCCTCC CC
//
Primer: PRE_rev
Sequence file: RDB15045_A7Enc.seq check
>D04276A3_A7En_1_PRE_rev_E02_14_ABI24.ab1
    1 AGTTTTCACA TAGCGTAAAG GAGCAACATA GTTAAGAATA CCAGTCAATC TTTCACAAAT
   61 TTTGTAATCC AGAGGTTGAG CGGCCGAAGG GCGAATTCGC GGCCGCTTAC TTAGTTACCC
  121 GGGGAGCATG TCAAGGTCAA AATCGTCAAG AGCGTCAGCA GGCAGCATAT CAAGGTCAAA
  181 GTCGTCAAGG GCATCGGCTG GGAGCATGTC TAAGTCAAAA TCGTCAAGGG CGTCGGCCGG
  241 CCCGCCGCTT TCGCACTTTA GCTGTTTCTC CAGGCCACAT ATGATTAGTT CCAGGCCGAA
  301 AAGGAAGGCA GGTTCGGCTC CCTGATGGTC GAACAGCTCA ATTGCTTGTC TCAGAAGTGG
  361 GGGCATAGAA TCGGTGGTAG GTGTCTCTCT TTCCTCTTTT GCTACTTGAT GCTCCTGATC
  421 CTCCAATACG CAGCCCAGTG TAAAGTGGCC CACGGCGGAC AGAGCGTACA GTGCGTTCTC
  481 CAGGGAGAAG CCTTGCTGAC ACAGGAACGC GAGCTGATTT TCCAGGGTTT CGTACTGTTT
  541 CTCTGTTGGG CGGGTGCCGA GATGCACTTT AGCCCCGTCG CGATGTGAGA GGAGAGCACA
  601 GCGGAATGAC TTGGCGTTGT TCCGCAGAAA GTCTTGCCAT GACTCGCCTT CCAGGGGGCA
  661 GAAGTGGGTA TGATGCCTGT CCAGCATCTC GATTGCCAGG GCATCGAGCA GGGCCCGCTT
  721 GTTCTTCACG TGCCAGTACA GGGTAGGCTG CTCAACTCCC AGCTTTTGAG CGAGTTTCCT
  781 TGTCGTCAGG CCTTCGATAC CGACTTCATT GAGTAATTCC AGAGCAGAGT TTATGACTTT
  841 GCTCTTGTCC AGTCTAGACA TCGTGTTTTT CAAAGGAAAC CACGTCCCCG TGGTTCGGGG
  901 GGCCTAGACG TTTTTTTAAC CTCGACTAAA CACATGTAAG CATGTGCACC GAGGCCCCAG
  961 ATCAGATCCC ATACATGGGG GTAACTTCTG GGCATCCCTT TCAGCCCCTT GTTGATACGC
 1021 TTTGAGAGAG CATTTACTCT TTCACACTAT CCAACTCACA CGTGTCACTG GGGATGTGCG
1081 CTTGCAGGGT GAATCCTAAA ACCACGTGAC CTTTTGC
//
Primer: SV40pro_F_V2
Sequence file: RDB15045_A7End.seq check
>D04276A3_A7En_1_SV40pro_F_V2_F02_17_ABI24.ab1
    1 GACACTTTTT GGAGGCTAGG CTTTTGCAAG ATCGATCAAG AGACAGGATG AGGATCGTTT
   61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC
  601 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
  661 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG
  721 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC
  781 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT
  841 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA
  901 ACCCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA
  961 ATCGTTTTCC GGACGCCGGC TGATGATCCC TCCAGCGCGG GATCTCATGC TGAGTTCTTC
 1021 GCCCACCCTA GGGGAGCTAC TGAACCACGG AATGAGAACA TACTGAAGAC CGCGCTATGA
1081 CCGCCAATTA AAGAACGTAA TAAAACGGTC G
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Mizuno, H., NMDAR-regulated dynamics of layer 4 neuronal dendrites during thalamocortical reorganization in neonates. Neuron 82 (2): 365-379 (2014). PMID 24685175.

2020.12.14

GNP_filter3_RDBDEP_html_201104.pl