Please review the QC test results indicated by icon below as well as clone information before placing your order.

pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

Cre-based Supernova series. Expression vector of loxP-stop-loxP-EGFP under the CAG promoter.

Sequence (full)	RDB15045zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Mizuno, H. et al. Neuron, 82: 365-379, 2014). Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

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Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Information of Request for Distribution.[link]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Mizuno, H. et al. Neuron, 82: 365-379, 2014)。 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15045_A7Enp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCALNLF1 (Pr0166) Region: loxP,insert 5' Sequence file: RDB15045_A7Ene.seq
>D04276A3_A7En_1_pAxCALNLF1_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNTNNTGTC TGGNNCTGAN NTGGTAAGTA AGCTTGGGCT GCAGGTCGAG    61 GGANCTAATA ACTTCGTATA GCATACATTA TACGAAGTTA TATGTCGACG CCACCATGGT   121 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA   181 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA   241 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT   301 GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA   361 CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA   421 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA   481 CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT   541 GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT   601 CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA   661 CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT   721 GAGCACCCAG TCCGCCCTGA GCAAAGANCC CAACGAGAAG CGCGATCACA TGGTCCTGCT   781 GGAGTTCGTG ACCGCCGCCG GNNTCACTCT CGGCATGGAC GANCTGTACA AGTAAGANAT   841 CCTAGATCGA GTTNATTNAN NNNTCNNCCC CCCCCCCCCN CNCCCNCCCC CCCCCCCNAA   901 CGTTACNGGN CNAAGCNNCT TGGAANNNNN NCNGNGNGCG TTNGNCNNNN TNTNATTTTN 961 NNNCNNANNG CCGTNTTTNG NNNNNGNGNN NNCNNNAANN NNNNNNNN //
Primer: mVenus-M_F (Pr0574) Region: insert 3',IRES Sequence file: RDB15045_A7Enf.seq
>15045_15045_B2CgF_mVenus-M_F_D01_04_ABI08.ab1     1 NNNNNNNNNN NNGGCNACGG CCCCGNGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG    61 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG   121 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAGATAT CCTAGATCGA   181 GTTAATTAAG GATTCCGCCC CCCCCCCTCT CCCTCCCCCC CCCCNNNNNN TACTGNNNNA   241 NNCCGCTTGG AATAAGGCCG GTGTGCGTTT GNCTNNNNGT TATTTTNNNN NNTATTGCCN 301 NNNTTTNNNN NNGNNNNGGC CCGNAANNCT GNNCCTGTCT TCTTGNCNAN NNNNNNNNN //
Primer: pAxCA_F1 (Pr0164) Region: loxP Sequence file: RDB15045_A7Eng.seq
>D04276A3_A7En_1_pAxCA_F1_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNGCCTT CTTCTTTTTC CNACNGCTCC TGGGCAACGT    61 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC TAATAACTTC GTATAGCATA   121 CATTATACGA AGTTATATTA AGGGTTCCGG ATCCTCGGGG ACACCAAATA TGGCGATCTC   181 GGCCTTTTCG TTTCTTGGAG CTGGGACATG TTTGCCATCG ATCCATCTAC CACCAGAACG   241 GCCGTTAGAT CTGCTGCCAC CGTTGTTTCC ACCGAAGAAA CCACCGTTGC CGTAACCACC   301 ACGACGGTTG TTGCTAAAGA AGCTGCCACC GCCACGGCCA CCGTTGTAGC CGCCGTTGTT   361 GTTATTGTAG TTGCTACTGT TATTTCTGGC ACTTCTTGGT TTTCCTCTTA AGTGAGGAGG   421 AACATAACCA TTCTCGTTGT TGTCGTTGAT GCTTAAATTT TGCACTTGTT CGCTCAGTTC   481 AGCCATAATA TGAAATGCTT TTCTTGTTGT TCTTACGGAA TACCACTTGC CACCTATCAC   541 CACAACTAAC TTTTTCCCGT TCCTCCATCT CTTTTATATT TTTTTTCTCG AGGGATCTTT   601 GTGAAGGAAC CTTACTTCTG TGGTGTGACA TAATTGGACA AACTACCTAC AGAGATTTAA   661 AGCTCTAANG TAAATATAAN ATTTTTAAGT GTATAATGTG TTAAACTACT GATTCTAATT   721 GTTTGTGTAT TTTANATTCC AACCTATGGA ACTGATGAAT GGGANCANTG GTGGAATGNC   781 TTTNATGANG AAAACCNGNT TNGCTNNNAN NAAANGNCNT CTANNGATGA NGNNNNTACN   841 GNNNANNNNC NNNNTTCNAC TNNNNNNAAA AANANNANAA NNNNANANNA NCCNNANNNA 901 NNTTNNNTNN NNNNNNNN //
Primer: PRE_rev (Pr0250) Region: WPRE,tTA Advanceed Sequence file: RDB15045_A7Enh.seq
>D04276A3_A7En_1_PRE_rev_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNCGNNNNG GANCNNCATA GTTAAGAATA CCAGTCAATC TTTCNCAAAT    61 TTTGTAATCC AGAGGTTGAG CGGCCGAAGG GCGAATTCGC GGCCGCTTAC TTAGTTACCC   121 GGGGAGCATG TCAAGGTCAA AATCGTCAAG AGCGTCAGCA GGCAGCATAT CAAGGTCAAA   181 GTCGTCAAGG GCATCGGCTG GGAGCATGTC TAAGTCAAAA TCGTCAAGGG CGTCGGCCGG   241 CCCGCCGCTT TCGCACTTTA GCTGTTTCTC CAGGCCACAT ATGATTAGTT CCAGGCCGAA   301 AAGGAAGGCA GGTTCGGCTC CCTGATGGTC GAACAGCTCA ATTGCTTGTC TCAGAAGTGG   361 GGGCATAGAA TCGGTGGTAG GTGTCTCTCT TTCCTCTTTT GCTACTTGAT GCTCCTGATC   421 CTCCAATACG CAGCCCAGTG TAAAGTGGCC CACGGCGGAC AGAGCGTACA GTGCGTTCTC   481 CAGGGAGAAG CCTTGCTGAC ACAGGAACGC GAGCTGATTT TCCAGGGTTT CGTACTGTTT   541 CTCTGTTGGG CGGGTGCCGA GATGCACTTT AGCCCCGTCG CGATGTGAGA GGAGAGCACA   601 GCGGAATGAC TTGGCGTTGT TCCGCAGAAA GTCTTGCCAT GACTCGCCTT CCAGGGGGCA   661 GAAGTGGGTA TGATGCCTGT CCAGCATCTC GATTGCCAGG GCATCGAGCA NGGCCCGCTT   721 GTTCTTCACG TGCCAGTACA GGGTANGCTG CTCNACTCCC ANCTTTTGAN CGAGTTTCCT   781 TGTCGTCAGG NCNTCNATAC CGACTTCNTT GANTAATTCC NGANCANANT TTATGANTTN   841 GCTCTTGNCC AGNCNANACA TCNNGTTTTT CNAANGAAAN NNCNNCCCNN NNNNNCGGGG 901 GNNNTANNNN TTTTTTNNNN NNNNNN //
Primer: SV40pro_F_V2 (Pr0154) Region: NeoR_KanR Sequence file: RDB15045_A7Eni.seq
>D04276A3_A7En_1_SV40pro_F_V2_F02_17_ABI24.ab1     1 NNNNNNNNNN NNNNNCNNNG CTTTTGNAAG NNCGATCNAG AGACAGGNTG AGGNNCGTTT    61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA   121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG   181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA   241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT   301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG   361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA   421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT   481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC   541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC   601 GACGGCGAGG NTCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA   661 ANTGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA NCGCTATCAN   721 GACATAGCGT TGGCTACCCG TGATATTGCT GAANANCTTG GCGGCGAATG GGCTGACCGC   781 TTCCTCGNGC TTTACGGTAT CGCCGCTCCC NANNCGCAGC GCATCGCCTT CTATCGCCNT   841 CNTGANNANT TCNTCTGANC GGGNNTNTGG GGTTCNAAAT GANNNANCNA GCGNCGCCNN 901 NNCNNNNNNT CNCNANNNTN CNATTNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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