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pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova) (#RDB15045)

Cre-based Supernova series. Expression vector of loxP-stop-loxP-EGFP under the CAG promoter.

Alternative name pK038
Clone info. Cre-based Supernova series. Expression vector of loxP-stop-loxP-EGFP under the CAG promoter. Cre-mediated recombination, a loxP-stop-loxP is excised and the downstream EGFP is expressed. The structure of the construct, see Fig.7 of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Comment Expression was confirmed by the depositor with immunohistochemistry and FACS.
Vector backbone pK029 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Depositor|Developer Iwasato, Takuji |
 
Sequence (full) RDB15045hts01.seq checkAssembled from experimentally sequenced data.
Publication Luo, W., Sci. Rep. 6: 35747 (2016). [link to RRC of NBRP]
Test sheet RDB15045_B2Cfp1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Mizuno, H. et al. Neuron, 82: 365-379, 2014).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Mizuno, H. et al. Neuron, 82: 365-379, 2014)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15045 pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15045).

Reference section:

Luo, W., Mizuno, H., Iwata, R., Nakazawa, S., Yasuda, K., Itohara, S., Iwasato, T., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045. [link to RRC of NBRP]