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pK031.TRE-Cre (Supernova)

Cre-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase.

Catalog number RDB15043
Resource name pK031.TRE-Cre (Supernova)
Alternative name pK031
Clone info. Cre-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase. This Cre expression vector will be used with pK029 (RDB15044), pk038 (RDB15045), pk098 (RDB15052) and pk177. Please refer Figs.4, 5 and 7 of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Comment Expression was confirmed by the depositor with immunohistochemistry and FACS.
Vector backbone pTRE-Tight (plasmid)
Selectable markers Amp^r (E. coli).
Gene/insert name Bacteriophage P1 Cre recombinase genomic DNA
Depositor|Developer Iwasato, Takuji |
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External Database
Bacteriophage P1 Cre recombinase

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Mizuno, H. et al. Neuron, 82: 365-379, 2014).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Mizuno, H. et al. Neuron, 82: 365-379, 2014)。
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            RDB15043 pK031.TRE-Cre (Supernova) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB15043zzk02.seq provided by the depositor

            Original reference

            original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045.
            original Mizuno, H., NMDAR-regulated dynamics of layer 4 neuronal dendrites during thalamocortical reorganization in neonates. Neuron 82 (2): 365-379 (2014). PMID 24685175.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15043_A7Enp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: Amp_R
            Region: AmpR pro,Tight TRE pro,insert 5'(SV40 NLS,Cre 5')
            Sequence file: RDB15043_A7Enc.seq check
            >D04276A1_A7En_1_Amp_R_A01_01_ABI24.ab1
                1 NNNNNNNNNN NNNNATTGAN CNNTTATNNG GGTTATTGTC TCATGAGCGG ANACATATTT
               61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
              121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
              181 AGGCCCTTTC GTCTTCACTC GAGTTTACTC CCTATCAGTG ATAGAGAACG TATGTCGAGT
              241 TTACTCCCTA TCAGTGATAG AGAACGATGT CGAGTTTACT CCCTATCAGT GATAGAGAAC
              301 GTATGTCGAG TTTACTCCCT ATCAGTGATA GAGAACGTAT GTCGAGTTTA CTCCCTATCA
              361 GTGATAGAGA ACGTATGTCG AGTTTATCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT
              421 ACTCCCTATC AGTGATAGAG AACGTATGTC GAGGTAGGCG TGTACGGTGG GAGGCCTATA
              481 TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG AATTCGCAGG ATCAGTCGAC
              541 CTGCAGCCCA AGCTGTACCC CCTCGAAACT GACAGGAGAA CCACCATGGG CCCAAAGAAG
              601 AAGAGAAAGG TTTCGAATTT ACTGACCGTA CACCAAAATT TGCCTGCATT ACCGGTCGAT
              661 GCAACGAGTG ATGAGGTTCG CAAGAACCTG ATGGACATGT TCAGGGATCG CCAGGCGTTT
              721 TCTGAGCATA CCTGGAAAAT GCTTCTGTCC GTTTGNCGGT CGTGGGCGGC ATGGTGCAAG
              781 TTGAATAACC GGAAATGGNT TCNCGCANAN NCTGAAGATG TTCGCGATTA TCTTCTATAT
              841 CTTCANGCGC GCGGNCTGGC AGTAAAAACT ATCCAGCAAC ATTTGGGCNN GCTAAACNTG
              901 CTTCATCGTN NGNNCNGNNN GCCNCGACCN ANTGANAGCA NNGCTGTTTN NCNNGNTTAT
            961 GCNGNNNANN CNAAAANAAA NGNTNGATGN CNGGNGNNNN NNNNAAANNN NNNNNNN
            //
            Primer: ME-1290RV
            Region: insert 3'(Cre 3')
            Sequence file: RDB15043_A7End.seq check
            >D04276A1_A7En_1_ME-1290RV_B01_04_ABI24.ab1
                1 NNNNNNNNNN NTNNNGTTTN NGGTTNNGGG GGNNGTGTGG GAGGTTTTTT AAAGCAAGTA
               61 AAACCTCTAC AAATGTGGTA TGGCTGATTA TGATCCTCTA GAGATATCGT CGACAAGCTT
              121 ATCGATGCGG CCGCGCGTTA ATGGCTAATC GCCATCTTCC AGCAGGCGCA CCATTGCCCC
              181 TGTTTCACTA TCCAGGTTAC GGATATAGTT CATGACAATA TTTACATTGG TCCAGCCACC
              241 AGCTTGCATG ATCTCCGGTA TTGAAACTCC AGCGCGGGCC ATATCTCGCG CGGCTCCGAC
              301 ACGGGCACTG TGTCCAGACC AGGCCAGGTA TCTCTGACCA NAGTCATCCT TAGCGCCGTA
              361 AATCAATCGA TGAGTTGCTT CAAAAATCCC TTCCAGGGCG CGAGTTGATA GCTGGCTGGT
              421 GGCANATGGC GCGGCAACAC CATTTTTTCT GACCCGGCAA AACAGGTAGT TATTCGGATC
              481 ATCAGCTACA CCANAGACGG AAATCCATCG CTCGACCAGT TTAGTTACCC CCAGGCTAAG
              541 TGCCTTCTCT ACACCTGCGG TGCTAACCAG CGTTTTCGTT CTGCCAATAT GGATTAACAT
              601 TCTCCCACCG TCAGTACGTG AGATATCTTT AACCCTGATC CTGGCAATTT CGGCTATACG
              661 TAACAGGGTG TTATAAGCAA TCCCCAGAAA TGCCAGATTA CGTATATCCT GNCAGCGATC
              721 GCTATTTTCC ATGAGTGAAC GAACCTGNTC GAAATCAGTG CGTTCGANCG CTAGAGCCTG
              781 TTTTGNACGT TCACCGGCAT CAACGTTTTC TTTTCGGATC CGCCGCATAA CCAGTGAAAC
              841 AGCATTGCTG TCANTNNNNC GNGGNAGCCC GGACCGANNA NGAANCNTGT TTANCTGNNC
              901 CAANNGTNNC TNGNTANNTT TTTACNGCNN NNNCNCNCNC NNGANNNNAN NNNNNANNNT
            961 CNNNANCANN NNTNNNNNNN NNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045.
            original Mizuno, H., NMDAR-regulated dynamics of layer 4 neuronal dendrites during thalamocortical reorganization in neonates. Neuron 82 (2): 365-379 (2014). PMID 24685175.

            2022.05.17

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