Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pK031.TRE-Cre (Supernova)

Cre-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase.

Catalog number RDB15043
Resource name pK031.TRE-Cre (Supernova)
Alternative name pK031
Clone info. Cre-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase. This Cre expression vector will be used with pK029 (RDB15044), pk038 (RDB15045), pk098 (RDB15052) and pk177. Please refer Figs.4, 5 and 7 of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Comment Expression was confirmed by the depositor with immunohistochemistry and FACS.
Vector backbone pTRE-Tight (plasmid)
Selectable markers Amp^r (E. coli).
Gene/insert name Bacteriophage P1 Cre recombinase genomic DNA
Depositor|Developer Iwasato, Takuji |
Other clones in our bank
External Database
Bacteriophage P1 Cre recombinase
            Reference sequence
              
             
            Sequence (full) RDB15043zzk02.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Mizuno, H. et al. Neuron, 82: 365-379, 2014).
            Ordering
            Order form [Credit Card Payment]  [Bank Transfer Payment]
            MTA, for use for not-for-profit academic purpose [Word]
            Please visit Information of Request for Distribution.[link] 
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Mizuno, H. et al. Neuron, 82: 365-379, 2014)。
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA, 非営利学術目的用)[Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15043 pK031.TRE-Cre (Supernova) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pK031.TRE-Cre (Supernova) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15043).

            Reference section:

            Luo, W., Mizuno, H., Iwata, R., Nakazawa, S., Yasuda, K., Itohara, S., Iwasato, T., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045. [link to RRC of NBRP]
            Mizuno, H., Luo, W., Tarusawa, E., Saito, Y.M., Sato, T., Yoshimura, Y., Itohara, S., Iwasato, T., NMDAR-regulated dynamics of layer 4 neuronal dendrites during thalamocortical reorganization in neonates. Neuron 82 (2): 365-379 (2014). PMID 24685175. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

            Tips

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)
            Featured content Expression regulation (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15043_A7Enp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: Amp_R (Pr0008)
            Region: AmpR pro,Tight TRE pro,insert 5'(SV40 NLS,Cre 5')
            Sequence file: RDB15043_A7Enc.seq check
            >D04276A1_A7En_1_Amp_R_A01_01_ABI24.ab1
                1 NNNNNNNNNN NNNNATTGAN CNNTTATNNG GGTTATTGTC TCATGAGCGG ANACATATTT
               61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
              121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
              181 AGGCCCTTTC GTCTTCACTC GAGTTTACTC CCTATCAGTG ATAGAGAACG TATGTCGAGT
              241 TTACTCCCTA TCAGTGATAG AGAACGATGT CGAGTTTACT CCCTATCAGT GATAGAGAAC
              301 GTATGTCGAG TTTACTCCCT ATCAGTGATA GAGAACGTAT GTCGAGTTTA CTCCCTATCA
              361 GTGATAGAGA ACGTATGTCG AGTTTATCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT
              421 ACTCCCTATC AGTGATAGAG AACGTATGTC GAGGTAGGCG TGTACGGTGG GAGGCCTATA
              481 TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG AATTCGCAGG ATCAGTCGAC
              541 CTGCAGCCCA AGCTGTACCC CCTCGAAACT GACAGGAGAA CCACCATGGG CCCAAAGAAG
              601 AAGAGAAAGG TTTCGAATTT ACTGACCGTA CACCAAAATT TGCCTGCATT ACCGGTCGAT
              661 GCAACGAGTG ATGAGGTTCG CAAGAACCTG ATGGACATGT TCAGGGATCG CCAGGCGTTT
              721 TCTGAGCATA CCTGGAAAAT GCTTCTGTCC GTTTGNCGGT CGTGGGCGGC ATGGTGCAAG
              781 TTGAATAACC GGAAATGGNT TCNCGCANAN NCTGAAGATG TTCGCGATTA TCTTCTATAT
              841 CTTCANGCGC GCGGNCTGGC AGTAAAAACT ATCCAGCAAC ATTTGGGCNN GCTAAACNTG
              901 CTTCATCGTN NGNNCNGNNN GCCNCGACCN ANTGANAGCA NNGCTGTTTN NCNNGNTTAT
            961 GCNGNNNANN CNAAAANAAA NGNTNGATGN CNGGNGNNNN NNNNAAANNN NNNNNNN
            //
            Primer: ME-1290RV (Pr0046)
            Region: insert 3'(Cre 3')
            Sequence file: RDB15043_A7End.seq check
            >D04276A1_A7En_1_ME-1290RV_B01_04_ABI24.ab1
                1 NNNNNNNNNN NTNNNGTTTN NGGTTNNGGG GGNNGTGTGG GAGGTTTTTT AAAGCAAGTA
               61 AAACCTCTAC AAATGTGGTA TGGCTGATTA TGATCCTCTA GAGATATCGT CGACAAGCTT
              121 ATCGATGCGG CCGCGCGTTA ATGGCTAATC GCCATCTTCC AGCAGGCGCA CCATTGCCCC
              181 TGTTTCACTA TCCAGGTTAC GGATATAGTT CATGACAATA TTTACATTGG TCCAGCCACC
              241 AGCTTGCATG ATCTCCGGTA TTGAAACTCC AGCGCGGGCC ATATCTCGCG CGGCTCCGAC
              301 ACGGGCACTG TGTCCAGACC AGGCCAGGTA TCTCTGACCA NAGTCATCCT TAGCGCCGTA
              361 AATCAATCGA TGAGTTGCTT CAAAAATCCC TTCCAGGGCG CGAGTTGATA GCTGGCTGGT
              421 GGCANATGGC GCGGCAACAC CATTTTTTCT GACCCGGCAA AACAGGTAGT TATTCGGATC
              481 ATCAGCTACA CCANAGACGG AAATCCATCG CTCGACCAGT TTAGTTACCC CCAGGCTAAG
              541 TGCCTTCTCT ACACCTGCGG TGCTAACCAG CGTTTTCGTT CTGCCAATAT GGATTAACAT
              601 TCTCCCACCG TCAGTACGTG AGATATCTTT AACCCTGATC CTGGCAATTT CGGCTATACG
              661 TAACAGGGTG TTATAAGCAA TCCCCAGAAA TGCCAGATTA CGTATATCCT GNCAGCGATC
              721 GCTATTTTCC ATGAGTGAAC GAACCTGNTC GAAATCAGTG CGTTCGANCG CTAGAGCCTG
              781 TTTTGNACGT TCACCGGCAT CAACGTTTTC TTTTCGGATC CGCCGCATAA CCAGTGAAAC
              841 AGCATTGCTG TCANTNNNNC GNGGNAGCCC GGACCGANNA NGAANCNTGT TTANCTGNNC
              901 CAANNGTNNC TNGNTANNTT TTTACNGCNN NNNCNCNCNC NNGANNNNAN NNNNNANNNT
            961 CNNNANCANN NNTNNNNNNN NNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045. [link to RRC of NBRP]
            original Mizuno, H., NMDAR-regulated dynamics of layer 4 neuronal dendrites during thalamocortical reorganization in neonates. Neuron 82 (2): 365-379 (2014). PMID 24685175. [link to RRC of NBRP]

            2024.03.26

            HTML_filter_RDB_240326.pl