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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pK031.TRE-Cre (Supernova)

Cre-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase.

Catalog number RDB15043
Resource name pK031.TRE-Cre (Supernova)
Alternative name pK031
Clone info. Cre-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase. This Cre expression vector will be used with pK029 (RDB15044), pk038 (RDB15045), pk098 (RDB15052) and pk177. Please refer Figs.4, 5 and 7 of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Expression was confirmed by the depositor with immunohistochemistry and FACS.
Vector backbone pTRE-Tight (plasmid)
Selectable markers Amp^r (E. coli).
Gene/insert name Bacteriophage P1 Cre recombinase genomic DNA
Depositor|Developer Iwasato, Takuji |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Mizuno, H. et al. Neuron, 82: 365-379, 2014).
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RDB15043 pK031.TRE-Cre (Supernova) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Mizuno, H., NMDAR-regulated dynamics of layer 4 neuronal dendrites during thalamocortical reorganization in neonates. Neuron 82 (2): 365-379 (2014). PMID 24685175.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15043_A7Enp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Amp_R
Sequence file: RDB15043_A7Ena.seq check
>D04276A1_A7En_1_Amp_R_A01_01_ABI24.ab1
    1 CCCCTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
  181 AGGCCCTTTC GTCTTCACTC GAGTTTACTC CCTATCAGTG ATAGAGAACG TATGTCGAGT
  241 TTACTCCCTA TCAGTGATAG AGAACGATGT CGAGTTTACT CCCTATCAGT GATAGAGAAC
  301 GTATGTCGAG TTTACTCCCT ATCAGTGATA GAGAACGTAT GTCGAGTTTA CTCCCTATCA
  361 GTGATAGAGA ACGTATGTCG AGTTTATCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT
  421 ACTCCCTATC AGTGATAGAG AACGTATGTC GAGGTAGGCG TGTACGGTGG GAGGCCTATA
  481 TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG AATTCGCAGG ATCAGTCGAC
  541 CTGCAGCCCA AGCTGTACCC CCTCGAAACT GACAGGAGAA CCACCATGGG CCCAAAGAAG
  601 AAGAGAAAGG TTTCGAATTT ACTGACCGTA CACCAAAATT TGCCTGCATT ACCGGTCGAT
  661 GCAACGAGTG ATGAGGTTCG CAAGAACCTG ATGGACATGT TCAGGGATCG CCAGGCGTTT
  721 TCTGAGCATA CCTGGAAAAT GCTTCTGTCC GTTTGCCGGT CGTGGGCGGC ATGGTGCAAG
  781 TTGAATAACC GGAAATGGTT TCCCGCAGAA CCTGAAGATG TTCGCGATTA TCTTCTATAT
  841 CTTCAGGCGC GCGGTCTGGC AGTAAAAACT ATCCAGCAAC ATTTGGGCCA GCTAAACATG
  901 CTTCATCGTC GGTCCGGGCT GCCACGACCA AGTGACAGCA ATGCTGTTTC ACTGGGTTAT
  961 GCGGCGGATC CGAAAAGAAA CGTTTGATGC CCGGTGAACG TGCAAAACAG GCTCTAGCGT
 1021 TCGAACGCAC TGATTTCGAC CAAGGTTCGT TTCAACTCAT GAAATAGCGA TCGCTGCAAG
 1081 AATATACGTA ATCTGCCATT TCTGGGATGC TATACACCCT GTTACGTATA GCCGAAAATT
1141 GTCCCAG
//
Primer: ME-1290RV
Sequence file: RDB15043_A7Enb.seq check
>D04276A1_A7En_1_ME-1290RV_B01_04_ABI24.ab1
    1 AGTCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA
   61 AAACCTCTAC AAATGTGGTA TGGCTGATTA TGATCCTCTA GAGATATCGT CGACAAGCTT
  121 ATCGATGCGG CCGCGCGTTA ATGGCTAATC GCCATCTTCC AGCAGGCGCA CCATTGCCCC
  181 TGTTTCACTA TCCAGGTTAC GGATATAGTT CATGACAATA TTTACATTGG TCCAGCCACC
  241 AGCTTGCATG ATCTCCGGTA TTGAAACTCC AGCGCGGGCC ATATCTCGCG CGGCTCCGAC
  301 ACGGGCACTG TGTCCAGACC AGGCCAGGTA TCTCTGACCA GAGTCATCCT TAGCGCCGTA
  361 AATCAATCGA TGAGTTGCTT CAAAAATCCC TTCCAGGGCG CGAGTTGATA GCTGGCTGGT
  421 GGCAGATGGC GCGGCAACAC CATTTTTTCT GACCCGGCAA AACAGGTAGT TATTCGGATC
  481 ATCAGCTACA CCAGAGACGG AAATCCATCG CTCGACCAGT TTAGTTACCC CCAGGCTAAG
  541 TGCCTTCTCT ACACCTGCGG TGCTAACCAG CGTTTTCGTT CTGCCAATAT GGATTAACAT
  601 TCTCCCACCG TCAGTACGTG AGATATCTTT AACCCTGATC CTGGCAATTT CGGCTATACG
  661 TAACAGGGTG TTATAAGCAA TCCCCAGAAA TGCCAGATTA CGTATATCCT GGCAGCGATC
  721 GCTATTTTCC ATGAGTGAAC GAACCTGGTC GAAATCAGTG CGTTCGAACG CTAGAGCCTG
  781 TTTTGCACGT TCACCGGCAT CAACGTTTTC TTTTCGGATC CGCCGCATAA CCAGTGAAAC
  841 AGCATTGCTG TCACTTGGTC GTGGCAGCCC GGACCGACGA TGAAGCATGT TTAGCTGGCC
  901 CAAATGTTGC TGGATAGTTT TTTACTGCCA GACCGCGCGC CTGAAGATAT AGAAGATAAT
  961 CGCGAACATT CTTTCAGGTT CTGCGGGAAA CCATTTCCGG TATTCAACTT TGCACCATTG
 1021 CCGCCCACGA CCGGCAACGG ACAGAAGCAA TTTTCCAGGT ATGGCTCAGA ACGCTGCGAT
 1081 TCCTGGACAT GTCCAATCAG TTCTTGCAAC CTCAATTCAC CTCTCGGTTG CCAATCTCGA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Mizuno, H., NMDAR-regulated dynamics of layer 4 neuronal dendrites during thalamocortical reorganization in neonates. Neuron 82 (2): 365-379 (2014). PMID 24685175.

2020.12.14

GNP_filter3_RDBDEP_html_201104.pl