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pEGFP-C1-human RAB3GAP1

Expression vector of human RAB3 GTPase activating protein catalytic subunit 1 (RAB3GAP1), fused with N-terminal EGFP, CMV promoter.

Catalog number RDB15026
Resource name pEGFP-C1-human RAB3GAP1
Alternative name KIAA0066
Clone info. Expression vector of human RAB3 GTPase activating protein catalytic subunit 1 (RAB3GAP1), fused with N-terminal EGFP, CMV promoter.
Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human RAB3GAP1 cDNA
Reference of nucleotide sequence AB449877 (DDBJ accession number)
Depositor Fukuda, Mitsunori |
 
Other clones in our bank human RAB3GAP1 (NCBI Gene 22930) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)
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Schedule A (GFP-A) [Link]
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RDB15026 pEGFP-C1-human RAB3GAP1 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15026_A7Egp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2
Sequence file: RDB15026_A7Ega.seq check
>D04556G2_A7Eg_1_EGFP-C2_C08_08_ABI24.ab1
    1 TACCCACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC
   61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCT ATGGCTGCCG ACAGTGAGCC
  121 CGAATCCGAG GTATTTGAGA TCACGGACTT CACCACTGCC TCGGAATGGG AAAGGTTTAT
  181 TTCCAAAGTT GAAGAAGTCT TGAATGACTG GAAACTGATT GGAAACTCTT TGGGAAAGCC
  241 ACTCGAAAAG GGTATATTTA CTTCTGGCAC ATGGGAAGAG AAATCAGATG AAATTTCCTT
  301 TGCTGACTTC AAGTTCTCAG TCACTCATCA TTATCTTGTA CAAGAGTCCA CTGATAAAGA
  361 AGGAAAGGAT GAGTTATTAG AGGATGTTGT TCCACAATCT ATGCAAGATT TGCTGGGTAT
  421 GAATAATGAC TTTCCTCCAA GAGCACATTG CCTGGTAAGA TGGTATGGGC TACGTGAGTT
  481 CGTGGTGATT GCCCCTGCTG CACACAGTGA CGCTGTTCTC AGCGAATCTA AGTGCAACCT
  541 TCTTCTGAGT TCTGTTTCTA TTGCCTTGGG AAACACTGGC TGTCAGGTGC CACTCTTTGT
  601 GCAAATTCAC CACAAATGGC GAAGAATGTA TGTAGGAGAA TGTCAAGGTC CTGGTGTACG
  661 AACTGATTTC GAAATGGTTC ATCTTAGAAA AGTGCCAAAT CAGTACACTC ACTTATCAGG
  721 TCTGCTGGAT ATCTTCAAAT CAAAGATTGG ATGTCCTTTA ACTCCATTGC CTCCAGTTAG
  781 TATTGCTATT CGATTTACCT ATGTACTTCA AGATTGGCAG CAGTATTTTT GGCCTCAGCA
  841 ACCTCCAGAC ATAGATGCCC TTGTAGGAGG AGAAGTTGGA GGCTTGGAGT TTGGCAAGTT
  901 ACCATTTGGT GCCTGCGAAG ATCCTATTAG TGAACTCCAT TTAGCTACTA CATGGCCCTC
  961 ATCTGACCGA ATGGATCATT GTGGATAATG ATGTTTATTC TGATTTGGGA TCCTATTTCA
 1021 AGCTCCACAT TGGTCTGTTA GAGTTTCGAA AAGCTGAGAA TCCTTCAGTG TTTGCCTAGG
 1081 TGATTTTTGT CACTTGAATT TTTTCAAAAT TTGCCCGTTC GAAGAGTTCG ACCGGATGGA
1141 ATCTGGACGA ATCTCGGCCA AAT
//
Primer: SV40polyA-R
Sequence file: RDB15026_A7Egb.seq check
>D04556G2_A7Eg_1_SV40polyA_R_A10_01_ABI24.ab1
    1 AAAAGTTGGA GTTTTTAAGC AAGTAAACCT CTACAAATGT GGTATGGCTG ATTATGATCA
   61 GTTATCTAGA TCCGGTGGAT CCCGGGCCCG CGGTACCGTC GACTGCAGAA TTCGAAGCTT
  121 GGTGCTGGGA AGTCTTTCCG ATGCTGGTTT GATTCCTGAT CACCCAACTG GACAGGGCCC
  181 TCTCCAGGAC CTCCCAGGTT CTCCCTGGTA CCTTCCCTCC CTCAGGAGGA GGCAGCACTG
  241 TCTCTGAAGC CACCAACGAG TAATGCTAGA AGAATCAGAA GAAGGAAGTA TCTGATGAAA
  301 AGGCACCTGC AAGTCTAAAG TCCTCTTTGG TGAGAACACT GTACATCCGC TGAGGCAGAG
  361 CTTTGGAGTA GGGAGCAGGG CGCGGCACAG TGGTGCGCAA AATGAATTCC CGGCCAGCAG
  421 GGGGTGGGAA GTCTGACACG GAGTTCTGCC TTCTTTCCTC TGGGGAGCCC ATTCTCTTCA
  481 ATTCCTCCTC TGGTGGAGTC ATAGCTGCAG CCCTCTGGGC ATTCACAAAC AGCTTGTGAA
  541 TGATCCTGCC AGCATGTCCT CTTCCTGCAC CGGTGACTAA CACTTCAGGC TGCTCCAGCA
  601 GGCAACTCAC AAACCTTTCA AGATCTTCCT TTTCCTCCTC CTGTTCACAT TTCTCAGTTC
  661 CAAACTTGGC TTTTAGTGAC CGAGCTCTGG CAATGAGAGC TTCCACATTA GTAATCTGGT
  721 GAATGATTTC TTCCAATTTC TTGTCTTCTG GATTGGGGAA GTGCAAAACT TTACTGGAAT
  781 GGGATATTAT CTGCTTTATG ATCTTCTTAA CTGAAGAAAT GTTTTCGAGA CTTTCTTCTT
  841 CCTTTACCTT GAGTACAGCT GCATGAATCA CACAAGGTAA CAGGTGCCGA GCAAGGTCTG
  901 CAGTTTCTGG ATTGCCAGAT AGTGCAGCAC CTTTTCTGCT TCCCGTGTAT CATCAAAGAG
  961 TCTCCCTTTG CCCTTCTAGC AGAAATTGGG CTTTAGCTGT TTCCCAGGCT TCTACCCACA
 1021 TATTGCTTTG GAATCATCAT CGGGGCACTC AGGTTCTCAT TCAGCACAAC ATGGCCGTTT
1081 CATCAATCAA CGTCGTGTCA ATATAATTCC CGGGGTGAAT ACCTAACCGT
//
Primer: SV40pro_F_V2
Sequence file: RDB15026_A7Egc.seq check
>D04556G2_A7Eg_1_SV40pro_F_V2_G11_20_ABI24.ab1
    1 GTGCAACCTT TTTTGGAGGC CTAGGCTTTT GCAAGATCGA TCAAGAGACA GGATGAGGAT
   61 CGTTTCGCAT GATCTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
  121 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
  181 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
  241 AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
  301 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
  361 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
  421 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
  481 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
  541 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC
  601 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
  661 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC
  721 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT
  781 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
  841 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGGT TCGAAATGAC CGACCAAGCG
  901 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCCTTCTATG AAAGGTTGGG
  961 GCTTTCGGAA TCGTTTTCCG GGACGCCCGC CTGGATGATC CTTCCAGCGC GGGATCTCAT
 1021 GCCTGGAGTT TCTTTCGGCC CCACCCTAGG GGGGAGGCTA AACTGAAACA CGAGAGACAT
1081 TAACCGGAAG GACCCGCCGC TATGAACGGC AATAAGGACA GAATTAAACG GTC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.

2019.12.12

GNP_filter3_RDBDEP_html_191130.pl