Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-human RAB3GAP1

Expression vector of human RAB3 GTPase activating protein catalytic subunit 1 (RAB3GAP1), fused with N-terminal EGFP, CMV promoter.

Catalog number RDB15026
Resource name pEGFP-C1-human RAB3GAP1
Alternative name KIAA0066
Clone info. Expression vector of human RAB3 GTPase activating protein catalytic subunit 1 (RAB3GAP1), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human RAB3GAP1 cDNA
Reference of insert sequence AB449877 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank

External Database
human RAB3GAP1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15026 pEGFP-C1-human RAB3GAP1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15026_A7Egp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2
          Sequence file: RDB15026_A7Ega.seq check
          >D04556G2_A7Eg_1_EGFP-C2_C08_08_ABI24.ab1
              1 TACCCACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC
             61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCT ATGGCTGCCG ACAGTGAGCC
            121 CGAATCCGAG GTATTTGAGA TCACGGACTT CACCACTGCC TCGGAATGGG AAAGGTTTAT
            181 TTCCAAAGTT GAAGAAGTCT TGAATGACTG GAAACTGATT GGAAACTCTT TGGGAAAGCC
            241 ACTCGAAAAG GGTATATTTA CTTCTGGCAC ATGGGAAGAG AAATCAGATG AAATTTCCTT
            301 TGCTGACTTC AAGTTCTCAG TCACTCATCA TTATCTTGTA CAAGAGTCCA CTGATAAAGA
            361 AGGAAAGGAT GAGTTATTAG AGGATGTTGT TCCACAATCT ATGCAAGATT TGCTGGGTAT
            421 GAATAATGAC TTTCCTCCAA GAGCACATTG CCTGGTAAGA TGGTATGGGC TACGTGAGTT
            481 CGTGGTGATT GCCCCTGCTG CACACAGTGA CGCTGTTCTC AGCGAATCTA AGTGCAACCT
            541 TCTTCTGAGT TCTGTTTCTA TTGCCTTGGG AAACACTGGC TGTCAGGTGC CACTCTTTGT
            601 GCAAATTCAC CACAAATGGC GAAGAATGTA TGTAGGAGAA TGTCAAGGTC CTGGTGTACG
            661 AACTGATTTC GAAATGGTTC ATCTTAGAAA AGTGCCAAAT CAGTACACTC ACTTATCAGG
            721 TCTGCTGGAT ATCTTCAAAT CAAAGATTGG ATGTCCTTTA ACTCCATTGC CTCCAGTTAG
            781 TATTGCTATT CGATTTACCT ATGTACTTCA AGATTGGCAG CAGTATTTTT GGCCTCAGCA
            841 ACCTCCAGAC ATAGATGCCC TTGTAGGAGG AGAAGTTGGA GGCTTGGAGT TTGGCAAGTT
            901 ACCATTTGGT GCCTGCGAAG ATCCTATTAG TGAACTCCAT TTAGCTACTA CATGGCCCTC
            961 ATCTGACCGA ATGGATCATT GTGGATAATG ATGTTTATTC TGATTTGGGA TCCTATTTCA
           1021 AGCTCCACAT TGGTCTGTTA GAGTTTCGAA AAGCTGAGAA TCCTTCAGTG TTTGCCTAGG
           1081 TGATTTTTGT CACTTGAATT TTTTCAAAAT TTGCCCGTTC GAAGAGTTCG ACCGGATGGA
          1141 ATCTGGACGA ATCTCGGCCA AAT
          //
          Primer: SV40polyA-R
          Sequence file: RDB15026_A7Egb.seq check
          >D04556G2_A7Eg_1_SV40polyA_R_A10_01_ABI24.ab1
              1 AAAAGTTGGA GTTTTTAAGC AAGTAAACCT CTACAAATGT GGTATGGCTG ATTATGATCA
             61 GTTATCTAGA TCCGGTGGAT CCCGGGCCCG CGGTACCGTC GACTGCAGAA TTCGAAGCTT
            121 GGTGCTGGGA AGTCTTTCCG ATGCTGGTTT GATTCCTGAT CACCCAACTG GACAGGGCCC
            181 TCTCCAGGAC CTCCCAGGTT CTCCCTGGTA CCTTCCCTCC CTCAGGAGGA GGCAGCACTG
            241 TCTCTGAAGC CACCAACGAG TAATGCTAGA AGAATCAGAA GAAGGAAGTA TCTGATGAAA
            301 AGGCACCTGC AAGTCTAAAG TCCTCTTTGG TGAGAACACT GTACATCCGC TGAGGCAGAG
            361 CTTTGGAGTA GGGAGCAGGG CGCGGCACAG TGGTGCGCAA AATGAATTCC CGGCCAGCAG
            421 GGGGTGGGAA GTCTGACACG GAGTTCTGCC TTCTTTCCTC TGGGGAGCCC ATTCTCTTCA
            481 ATTCCTCCTC TGGTGGAGTC ATAGCTGCAG CCCTCTGGGC ATTCACAAAC AGCTTGTGAA
            541 TGATCCTGCC AGCATGTCCT CTTCCTGCAC CGGTGACTAA CACTTCAGGC TGCTCCAGCA
            601 GGCAACTCAC AAACCTTTCA AGATCTTCCT TTTCCTCCTC CTGTTCACAT TTCTCAGTTC
            661 CAAACTTGGC TTTTAGTGAC CGAGCTCTGG CAATGAGAGC TTCCACATTA GTAATCTGGT
            721 GAATGATTTC TTCCAATTTC TTGTCTTCTG GATTGGGGAA GTGCAAAACT TTACTGGAAT
            781 GGGATATTAT CTGCTTTATG ATCTTCTTAA CTGAAGAAAT GTTTTCGAGA CTTTCTTCTT
            841 CCTTTACCTT GAGTACAGCT GCATGAATCA CACAAGGTAA CAGGTGCCGA GCAAGGTCTG
            901 CAGTTTCTGG ATTGCCAGAT AGTGCAGCAC CTTTTCTGCT TCCCGTGTAT CATCAAAGAG
            961 TCTCCCTTTG CCCTTCTAGC AGAAATTGGG CTTTAGCTGT TTCCCAGGCT TCTACCCACA
           1021 TATTGCTTTG GAATCATCAT CGGGGCACTC AGGTTCTCAT TCAGCACAAC ATGGCCGTTT
          1081 CATCAATCAA CGTCGTGTCA ATATAATTCC CGGGGTGAAT ACCTAACCGT
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB15026_A7Egc.seq check
          >D04556G2_A7Eg_1_SV40pro_F_V2_G11_20_ABI24.ab1
              1 GTGCAACCTT TTTTGGAGGC CTAGGCTTTT GCAAGATCGA TCAAGAGACA GGATGAGGAT
             61 CGTTTCGCAT GATCTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
            121 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
            181 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
            241 AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
            301 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
            361 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
            421 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
            481 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
            541 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC
            601 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
            661 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC
            721 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT
            781 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
            841 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGGT TCGAAATGAC CGACCAAGCG
            901 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCCTTCTATG AAAGGTTGGG
            961 GCTTTCGGAA TCGTTTTCCG GGACGCCCGC CTGGATGATC CTTCCAGCGC GGGATCTCAT
           1021 GCCTGGAGTT TCTTTCGGCC CCACCCTAGG GGGGAGGCTA AACTGAAACA CGAGAGACAT
          1081 TAACCGGAAG GACCCGCCGC TATGAACGGC AATAAGGACA GAATTAAACG GTC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.

          2022.07.13

          GNP_filter3_RDBDEP_html_220713.pl