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pAcGFP1-N1-RvLEAM (#RDB14656)

Expression vector of GFP-fused water bear (Ramazzottius varieornatus) Late embryogenesis abundant from mitochondria (RvLEAM).

Clone info. Expression vector of GFP (AcGFP)-fused water bear (Ramazzottius varieornatus) Late embryogenesis abundant from mitochondria (RvLEAM) under the CMV promoter for transient expression in mammalian cells.
Comment Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pAcGFP1-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Ramazzottius varieornatus RvLEAM cDNA
Reference of insert sequence LC002821 (DDBJ accession number)
Depositor|Developer Kunieda, Takekazu |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.（Tanaka, S et al. PLoS One, 10(2): e0118272, 2015)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって開発者の指定する文献を引用する（Tanaka, S et al. PLoS One, 10(2): e0118272, 2015)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14656 pAcGFP1-N1-RvLEAM Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pAcGFP1-N1-RvLEAM was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14656).

Reference section:

Tanaka, S., Tanaka, J., Miwa, Y., Horikawa, D.D., Katayama, T., Arakawa, K., Toyoda, A., Kubo, T., Kunieda, T., Novel mitochondria-targeted heat-soluble proteins identified in the anhydrobiotic Tardigrade improve osmotic tolerance of human cells. PLoS One 10 (2): e0118272 (2015). PMID 25675104. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Tanaka, S., Novel mitochondria-targeted heat-soluble proteins identified in the anhydrobiotic Tardigrade improve osmotic tolerance of human cells. PLoS One 10 (2): e0118272 (2015). PMID 25675104. [link to RRC of NBRP]