Please review the QC test results indicated by icon below as well as clone information before placing your order.

pAcGFP1-N1-CAHS1

Expression vector of GFP-fused water bear (Ramazzottius varieornatus) Cytoplasmic Abundant Heat Soluble 1 (CAHS1).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.（Yamaguchi, A et al. PLoS One, 7(8): e44209, 2012)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
Remarks	This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって開発者の指定する文献を引用する（Yamaguchi, A et al. PLoS One, 7(8): e44209, 2012)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
備考	Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。ライセンス料相当額を負担して頂きます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14651_A7Cfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro,insert 5'(CAHS1 5') Sequence file: RDB14651_A7Cfa.seq
>D04435A6_A7Cf_1_CMV-Forward_B04_04_ABI24.ab1     1 NNNNNNNNCN NNNNNTGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTCGAGCTC   121 AAGCTTCGAA TTCTGCAGTC GACGGTACCA TGCCTTACGA AAAGCACGTT GAACAGACGG   181 TGGTGGAAAA AACTGAGCAG CCTGGACACT CGTCGACGCA CCATGCTCCC GCCCAAAGGA   241 CCGTAGCTCG CGAGCAGGAG GAAGTTGTCC ACAAAGAGTT TACCCATACC GACATTCGAG   301 TTCCCCACAT CGACGCACCT CCTCCAATCA TCGCTGCCAG CGCAGTGGGC TTGGCCGAGG   361 AGATCGTCAG TCATGGTTTC CAAGCCTCGG CGGCCCGCAT CAGCGGTGCT TCCACCGAGG   421 TCGATATGCG CCCAAGTCCC AAGCTAGCCG AGGAAGCTCG TCGTGATGCC GAACGATATC   481 AAAAGGAGCA CGAGATGATC AACAGACAAG CCGAAGCCAC GCTGCAAAAG AAGGCGGAGG   541 AGTACCGTCA CCAGACTGAG GCGGAAGCCG AGAAGATTCG CCGCGAACTG GAAAAGCAGC   601 ATGAACGGGA CATCCAGTTC AGGAAGGACC TCATCGATCA GACCATCGAA AAGCAGAAGC   661 GCGAAGTTGA TCTGGAAGCC AAGATGGCCA AACGCGAGCT GGATCGCGAA GCGCAGTTGG   721 CTAANGAAGC CCTGGAACGT TCTCGAATGG CCACCAACGT GGAAGTCACG CTGGATACCG   781 CAGCGGGACA TACAGTAAGT NNNGGAACTA CNGTCTCCNG CGTCNANAAA NTTGAAACCG   841 TCCGCGANCG CAAACATCAT GATCCACCGG NCATGGNNNN NNANNGNNGC CNANCNNNTC 901 NCCGGNATCN NNNCNNTCCN NNTCNNNCNN NNNNGNNATN NNNNNNNNN //
Primer: QUEEN-M_R Region: AcGFP1 5',insert 3'(CAHS1 3') Sequence file: RDB14651_A7Cfb.seq
>14651_14651_B2AiF_QUEEN-M_R_D02_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNT NNCGCTGANC TTGTGGCCNT TCNCATCGCC NTTCAGCTCG    61 ATCAGGATGG GCACGATGCC GGTGAACAGC TCGGCGCCCT TGCTCACCAT GACCGGTGGA   121 TCATGATGTT TGCGCTCGCG GACGGTTTCA ACTTTGTCGA CGCTGGAGAC GGTAGTTCCC   181 CCACTTACTG TATGTCCCGC TGCGGTATCC AGCGTGACTT CCACGTTGGT GGCCATTCGA   241 GAACGTTCCA GGGCTTCCTT AGCCAACTGC GCTTCGCGAT CCAGCTCGCG TTTGGCCATC   301 TTGGCTTCCA GATCAACTTC GCGCTTCTGC TTTTCGATGG TCTGATCGAT GAGGTCCTTC   361 CTGAACTGGA TGTCCCGTTC ATGCTGCTTT TCCAGTTCGC GGCGAATCTT CTCGGCTTCC   421 GCCTCAGTCT GGTGACGGTA CTCCTCCGCC TTCTTTTGCA GCGTGGCTTC GGCTTGTCTG   481 TTGATCATCT CGTGCTCCTT TTGATATCGT TCGGCATCAC GACGAGCTTC CTCGGCTAGC   541 TTGGGACTTG GGCGCATATC GACCTCGGTG GAAGCACCGC TGATGCGGGC CGCCGANGCT   601 TGNAAACCAT GACTGACGAT CTCCTCGGNC NAGCCNACTG CGCTGGCAGC GATGANTGGA   661 GGANGTGCGT CNATGTGGGG ANCTCNANNG NCNGTATGGG TAANCTCTTN GNGGANNNNN 721 TNNNNNNGNT CNNNNNNNNN N //
Primer: SV40polyA-R Region: AcGFP1 3 ' Sequence file: RDB14651_A7Cfc.seq
>D04435A6_A7Cf_1_SV40polyA-R_B05_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNAGTN NANCTCTACN AATGTGGTAT GGCTGATTAT GATCTAGAGT    61 CGCGGCCGCT CACTTGTACA GCTCATCCAT GCCGTGGGTG ATGGCGGCGG CGGTCACGAA   121 GCCGAAGTAG ATCATGTGAT CGCGCTTCTC GTTGGGGTCC TTGGACAGGG CGCTCTGGGT   181 GGACAGGTAG TGGTTATCGG GCAGCAGCAC AGGGCCATCG CCGATGGGGG TATTCTGCTG   241 GTAGTGGTCG GCCAGCTGCA CGCTGCCATC CTCGATGTTG TGGCGGATCT TGAAGTTCAC   301 CTTGATGCCA TTCTTGGCCT TGTCGGTCAT GATGTACACA TTGTGGGCGT TGTAGTTGTA   361 CTCCATCTTA TTGCCCAGGA TGTTGCCATC CTCCTTGAAA TCGGTGCCGG TCAGCTCGAT   421 GCGATTCACC AGGGTATCGC CCTCGAACTT CACCTCGGCG CGCGACTTGT AGTTGCCGTC   481 ATCCTCGAAG AAGATGGTGC GCTCCTGGAT GTAGCCCTCA GGCATGGCGC TCTTGAAGAA   541 GTCGTGCTGC TTCATGTGAT CGGGGTAGCG TGAGAAGCAC TGCACGCCGT AGCTCAGGGT   601 GGTCACCAGG GTGGGCCAGG GCACAGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT   661 CAGCTTGCCG TANGTGNCAT CGCCCTCGCC CTCGCCGCTC ACGCTGAACT TGTGNCCATT   721 CACATCGCCA TTCAGCTCGA TCAGGANGGG CACGATGCCN GTGAACAGCT CGGCGCCCTT   781 GCTCACCATG ACCGGNGGAN CATGANGTTT GCGCTCGCGG ANNGNTTCNA CTTTGTCGAN   841 GCTNGANNNN GNAGTTCCCC CACTTACTGN ATGNCCCGCT GCGGNATNCA GCNNNNCTTN   901 CNCGTTGGNG GNNNTTCNAN NNNNTTNCNN GNCTNNCNNN NNNCNNNNNG NNCNTNNNNN 961 NNNNN //
Primer: SV40pro_F_V2 Region: Neo R_Kan R Sequence file: RDB14651_A7Cfd.seq
>D04435A6_A7Cf_1_SV40pro_F_V2_B06_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GCTTTTGNNN NNNCGATCAA GAGANAGGNN GAGGNNCGTT    61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT   121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT   181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA   241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC   301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG   361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC   421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA   481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA   541 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC   601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA   661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA   721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGANCG   781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT   841 TCTTGACNAN TTCTTCTGAN CGGGACTCTN GGGTNCNAAA TGACCNANCN ANCGACGCCN   901 NACNNGCCNT CACNANNNTT CNANTNNNCC NNCNNCNTTC NNNNAANNNN NGGNNTNNNN 961 NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl