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pAcGFP1-N1-CAHS1

Expression vector of GFP-fused water bear (Ramazzottius varieornatus) Cytoplasmic Abundant Heat Soluble 1 (CAHS1).

Catalog number RDB14651
Resource name pAcGFP1-N1-CAHS1
Clone info. Expression vector of GFP (AcGFP)-fused water bear (Ramazzottius varieornatus) Cytoplasmic Abundant Heat Soluble 1 (CAHS1) under the CMV promoter for transient expression in mammalian cells.
Comment Expression was confirmed by the depositor with SDS-PAGE and confocal microscopy.
Vector backbone pAcGFP1-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Ramazzottius varieornatus CAHS1 cDNA
Reference of insert sequence AB650499 (DDBJ accession number)
Depositor|Developer Kunieda, Takekazu |
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External Database
Ramazzottius varieornatus CAHS1
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.（Yamaguchi, A et al. PLoS One, 7(8): e44209, 2012)
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            提供条件 利用者は、研究成果の公表にあたって開発者の指定する文献を引用する（Yamaguchi, A et al. PLoS One, 7(8): e44209, 2012)。
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            RDB14651 pAcGFP1-N1-CAHS1 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pAcGFP1-N1-CAHS1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14651).

            Reference section:

            Yamaguchi, A., Tanaka, S., Yamaguchi, S., Kuwahara, H., Takamura, C., Imajoh-Ohmi, S., Horikawa, D.D., Toyoda, A., Katayama, T., Arakawa, K., Fujiyama, A., Kubo, T., Kunieda, T., Two novel heat-soluble protein families abundantly expressed in an anhydrobiotic tardigrade. PLoS One 7 (8): e44209 (2012). PMID 22937162. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14651_A7Cfp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward (Pr0016)
            Region: CMV pro,insert 5'(CAHS1 5')
            Sequence file: RDB14651_A7Cfa.seq check
            >D04435A6_A7Cf_1_CMV-Forward_B04_04_ABI24.ab1
                1 NNNNNNNNCN NNNNNTGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
               61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTCGAGCTC
              121 AAGCTTCGAA TTCTGCAGTC GACGGTACCA TGCCTTACGA AAAGCACGTT GAACAGACGG
              181 TGGTGGAAAA AACTGAGCAG CCTGGACACT CGTCGACGCA CCATGCTCCC GCCCAAAGGA
              241 CCGTAGCTCG CGAGCAGGAG GAAGTTGTCC ACAAAGAGTT TACCCATACC GACATTCGAG
              301 TTCCCCACAT CGACGCACCT CCTCCAATCA TCGCTGCCAG CGCAGTGGGC TTGGCCGAGG
              361 AGATCGTCAG TCATGGTTTC CAAGCCTCGG CGGCCCGCAT CAGCGGTGCT TCCACCGAGG
              421 TCGATATGCG CCCAAGTCCC AAGCTAGCCG AGGAAGCTCG TCGTGATGCC GAACGATATC
              481 AAAAGGAGCA CGAGATGATC AACAGACAAG CCGAAGCCAC GCTGCAAAAG AAGGCGGAGG
              541 AGTACCGTCA CCAGACTGAG GCGGAAGCCG AGAAGATTCG CCGCGAACTG GAAAAGCAGC
              601 ATGAACGGGA CATCCAGTTC AGGAAGGACC TCATCGATCA GACCATCGAA AAGCAGAAGC
              661 GCGAAGTTGA TCTGGAAGCC AAGATGGCCA AACGCGAGCT GGATCGCGAA GCGCAGTTGG
              721 CTAANGAAGC CCTGGAACGT TCTCGAATGG CCACCAACGT GGAAGTCACG CTGGATACCG
              781 CAGCGGGACA TACAGTAAGT NNNGGAACTA CNGTCTCCNG CGTCNANAAA NTTGAAACCG
              841 TCCGCGANCG CAAACATCAT GATCCACCGG NCATGGNNNN NNANNGNNGC CNANCNNNTC
            901 NCCGGNATCN NNNCNNTCCN NNTCNNNCNN NNNNGNNATN NNNNNNNNN
            //
            Primer: QUEEN-M_R (Pr0808)
            Region: AcGFP1 5',insert 3'(CAHS1 3')
            Sequence file: RDB14651_A7Cfb.seq check
            >14651_14651_B2AiF_QUEEN-M_R_D02_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNT NNCGCTGANC TTGTGGCCNT TCNCATCGCC NTTCAGCTCG
               61 ATCAGGATGG GCACGATGCC GGTGAACAGC TCGGCGCCCT TGCTCACCAT GACCGGTGGA
              121 TCATGATGTT TGCGCTCGCG GACGGTTTCA ACTTTGTCGA CGCTGGAGAC GGTAGTTCCC
              181 CCACTTACTG TATGTCCCGC TGCGGTATCC AGCGTGACTT CCACGTTGGT GGCCATTCGA
              241 GAACGTTCCA GGGCTTCCTT AGCCAACTGC GCTTCGCGAT CCAGCTCGCG TTTGGCCATC
              301 TTGGCTTCCA GATCAACTTC GCGCTTCTGC TTTTCGATGG TCTGATCGAT GAGGTCCTTC
              361 CTGAACTGGA TGTCCCGTTC ATGCTGCTTT TCCAGTTCGC GGCGAATCTT CTCGGCTTCC
              421 GCCTCAGTCT GGTGACGGTA CTCCTCCGCC TTCTTTTGCA GCGTGGCTTC GGCTTGTCTG
              481 TTGATCATCT CGTGCTCCTT TTGATATCGT TCGGCATCAC GACGAGCTTC CTCGGCTAGC
              541 TTGGGACTTG GGCGCATATC GACCTCGGTG GAAGCACCGC TGATGCGGGC CGCCGANGCT
              601 TGNAAACCAT GACTGACGAT CTCCTCGGNC NAGCCNACTG CGCTGGCAGC GATGANTGGA
              661 GGANGTGCGT CNATGTGGGG ANCTCNANNG NCNGTATGGG TAANCTCTTN GNGGANNNNN
            721 TNNNNNNGNT CNNNNNNNNN N
            //
            Primer: SV40polyA-R (Pr0109)
            Region: AcGFP1 3 '
            Sequence file: RDB14651_A7Cfc.seq check
            >D04435A6_A7Cf_1_SV40polyA-R_B05_05_ABI24.ab1
                1 NNNNNNNNNN NNNNNNAGTN NANCTCTACN AATGTGGTAT GGCTGATTAT GATCTAGAGT
               61 CGCGGCCGCT CACTTGTACA GCTCATCCAT GCCGTGGGTG ATGGCGGCGG CGGTCACGAA
              121 GCCGAAGTAG ATCATGTGAT CGCGCTTCTC GTTGGGGTCC TTGGACAGGG CGCTCTGGGT
              181 GGACAGGTAG TGGTTATCGG GCAGCAGCAC AGGGCCATCG CCGATGGGGG TATTCTGCTG
              241 GTAGTGGTCG GCCAGCTGCA CGCTGCCATC CTCGATGTTG TGGCGGATCT TGAAGTTCAC
              301 CTTGATGCCA TTCTTGGCCT TGTCGGTCAT GATGTACACA TTGTGGGCGT TGTAGTTGTA
              361 CTCCATCTTA TTGCCCAGGA TGTTGCCATC CTCCTTGAAA TCGGTGCCGG TCAGCTCGAT
              421 GCGATTCACC AGGGTATCGC CCTCGAACTT CACCTCGGCG CGCGACTTGT AGTTGCCGTC
              481 ATCCTCGAAG AAGATGGTGC GCTCCTGGAT GTAGCCCTCA GGCATGGCGC TCTTGAAGAA
              541 GTCGTGCTGC TTCATGTGAT CGGGGTAGCG TGAGAAGCAC TGCACGCCGT AGCTCAGGGT
              601 GGTCACCAGG GTGGGCCAGG GCACAGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT
              661 CAGCTTGCCG TANGTGNCAT CGCCCTCGCC CTCGCCGCTC ACGCTGAACT TGTGNCCATT
              721 CACATCGCCA TTCAGCTCGA TCAGGANGGG CACGATGCCN GTGAACAGCT CGGCGCCCTT
              781 GCTCACCATG ACCGGNGGAN CATGANGTTT GCGCTCGCGG ANNGNTTCNA CTTTGTCGAN
              841 GCTNGANNNN GNAGTTCCCC CACTTACTGN ATGNCCCGCT GCGGNATNCA GCNNNNCTTN
              901 CNCGTTGGNG GNNNTTCNAN NNNNTTNCNN GNCTNNCNNN NNNCNNNNNG NNCNTNNNNN
            961 NNNNN
            //
            Primer: SV40pro_F_V2 (Pr0154)
            Region: Neo R_Kan R
            Sequence file: RDB14651_A7Cfd.seq check
            >D04435A6_A7Cf_1_SV40pro_F_V2_B06_06_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN GCTTTTGNNN NNNCGATCAA GAGANAGGNN GAGGNNCGTT
               61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
              121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
              181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
              241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
              301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
              361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
              421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
              481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
              541 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
              601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
              661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
              721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGANCG
              781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
              841 TCTTGACNAN TTCTTCTGAN CGGGACTCTN GGGTNCNAAA TGACCNANCN ANCGACGCCN
              901 NACNNGCCNT CACNANNNTT CNANTNNNCC NNCNNCNTTC NNNNAANNNN NGGNNTNNNN
            961 NNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Yamaguchi, A., Two novel heat-soluble protein families abundantly expressed in an anhydrobiotic tardigrade. PLoS One 7 (8): e44209 (2012). PMID 22937162. [link to RRC of NBRP]

            2023.12.13

            GNP_filter3_RDBDEP_html_231213.pl