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pAcGFP1-N1-CAHS1 (#RDB14651)

Expression vector of GFP-fused water bear (Ramazzottius varieornatus) Cytoplasmic Abundant Heat Soluble 1 (CAHS1).

Clone info. Expression vector of GFP (AcGFP)-fused water bear (Ramazzottius varieornatus) Cytoplasmic Abundant Heat Soluble 1 (CAHS1) under the CMV promoter for transient expression in mammalian cells.
Comment Expression was confirmed by the depositor with SDS-PAGE and confocal microscopy.
Vector backbone pAcGFP1-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Ramazzottius varieornatus CAHS1 cDNA
Reference of insert sequence AB650499 (DDBJ accession number)
Depositor|Developer Kunieda, Takekazu |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.（Yamaguchi, A et al. PLoS One, 7(8): e44209, 2012)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって開発者の指定する文献を引用する（Yamaguchi, A et al. PLoS One, 7(8): e44209, 2012)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14651 pAcGFP1-N1-CAHS1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAcGFP1-N1-CAHS1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14651).

Reference section:

Yamaguchi, A., Tanaka, S., Yamaguchi, S., Kuwahara, H., Takamura, C., Imajoh-Ohmi, S., Horikawa, D.D., Toyoda, A., Katayama, T., Arakawa, K., Fujiyama, A., Kubo, T., Kunieda, T., Two novel heat-soluble protein families abundantly expressed in an anhydrobiotic tardigrade. PLoS One 7 (8): e44209 (2012). PMID 22937162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14651_A7Cfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert 5'(CAHS1 5')
Sequence file: RDB14651_A7Cfa.seq check
>D04435A6_A7Cf_1_CMV-Forward_B04_04_ABI24.ab1
    1 NNNNNNNNCN NNNNNTGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTCGAGCTC
  121 AAGCTTCGAA TTCTGCAGTC GACGGTACCA TGCCTTACGA AAAGCACGTT GAACAGACGG
  181 TGGTGGAAAA AACTGAGCAG CCTGGACACT CGTCGACGCA CCATGCTCCC GCCCAAAGGA
  241 CCGTAGCTCG CGAGCAGGAG GAAGTTGTCC ACAAAGAGTT TACCCATACC GACATTCGAG
  301 TTCCCCACAT CGACGCACCT CCTCCAATCA TCGCTGCCAG CGCAGTGGGC TTGGCCGAGG
  361 AGATCGTCAG TCATGGTTTC CAAGCCTCGG CGGCCCGCAT CAGCGGTGCT TCCACCGAGG
  421 TCGATATGCG CCCAAGTCCC AAGCTAGCCG AGGAAGCTCG TCGTGATGCC GAACGATATC
  481 AAAAGGAGCA CGAGATGATC AACAGACAAG CCGAAGCCAC GCTGCAAAAG AAGGCGGAGG
  541 AGTACCGTCA CCAGACTGAG GCGGAAGCCG AGAAGATTCG CCGCGAACTG GAAAAGCAGC
  601 ATGAACGGGA CATCCAGTTC AGGAAGGACC TCATCGATCA GACCATCGAA AAGCAGAAGC
  661 GCGAAGTTGA TCTGGAAGCC AAGATGGCCA AACGCGAGCT GGATCGCGAA GCGCAGTTGG
  721 CTAANGAAGC CCTGGAACGT TCTCGAATGG CCACCAACGT GGAAGTCACG CTGGATACCG
  781 CAGCGGGACA TACAGTAAGT NNNGGAACTA CNGTCTCCNG CGTCNANAAA NTTGAAACCG
  841 TCCGCGANCG CAAACATCAT GATCCACCGG NCATGGNNNN NNANNGNNGC CNANCNNNTC
901 NCCGGNATCN NNNCNNTCCN NNTCNNNCNN NNNNGNNATN NNNNNNNNN
//
Primer: QUEEN-M_R (Pr0808)
Region: AcGFP1 5',insert 3'(CAHS1 3')
Sequence file: RDB14651_A7Cfb.seq check
>14651_14651_B2AiF_QUEEN-M_R_D02_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNT NNCGCTGANC TTGTGGCCNT TCNCATCGCC NTTCAGCTCG
   61 ATCAGGATGG GCACGATGCC GGTGAACAGC TCGGCGCCCT TGCTCACCAT GACCGGTGGA
  121 TCATGATGTT TGCGCTCGCG GACGGTTTCA ACTTTGTCGA CGCTGGAGAC GGTAGTTCCC
  181 CCACTTACTG TATGTCCCGC TGCGGTATCC AGCGTGACTT CCACGTTGGT GGCCATTCGA
  241 GAACGTTCCA GGGCTTCCTT AGCCAACTGC GCTTCGCGAT CCAGCTCGCG TTTGGCCATC
  301 TTGGCTTCCA GATCAACTTC GCGCTTCTGC TTTTCGATGG TCTGATCGAT GAGGTCCTTC
  361 CTGAACTGGA TGTCCCGTTC ATGCTGCTTT TCCAGTTCGC GGCGAATCTT CTCGGCTTCC
  421 GCCTCAGTCT GGTGACGGTA CTCCTCCGCC TTCTTTTGCA GCGTGGCTTC GGCTTGTCTG
  481 TTGATCATCT CGTGCTCCTT TTGATATCGT TCGGCATCAC GACGAGCTTC CTCGGCTAGC
  541 TTGGGACTTG GGCGCATATC GACCTCGGTG GAAGCACCGC TGATGCGGGC CGCCGANGCT
  601 TGNAAACCAT GACTGACGAT CTCCTCGGNC NAGCCNACTG CGCTGGCAGC GATGANTGGA
  661 GGANGTGCGT CNATGTGGGG ANCTCNANNG NCNGTATGGG TAANCTCTTN GNGGANNNNN
721 TNNNNNNGNT CNNNNNNNNN N
//
Primer: SV40polyA-R (Pr0109)
Region: AcGFP1 3 '
Sequence file: RDB14651_A7Cfc.seq check
>D04435A6_A7Cf_1_SV40polyA-R_B05_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNAGTN NANCTCTACN AATGTGGTAT GGCTGATTAT GATCTAGAGT
   61 CGCGGCCGCT CACTTGTACA GCTCATCCAT GCCGTGGGTG ATGGCGGCGG CGGTCACGAA
  121 GCCGAAGTAG ATCATGTGAT CGCGCTTCTC GTTGGGGTCC TTGGACAGGG CGCTCTGGGT
  181 GGACAGGTAG TGGTTATCGG GCAGCAGCAC AGGGCCATCG CCGATGGGGG TATTCTGCTG
  241 GTAGTGGTCG GCCAGCTGCA CGCTGCCATC CTCGATGTTG TGGCGGATCT TGAAGTTCAC
  301 CTTGATGCCA TTCTTGGCCT TGTCGGTCAT GATGTACACA TTGTGGGCGT TGTAGTTGTA
  361 CTCCATCTTA TTGCCCAGGA TGTTGCCATC CTCCTTGAAA TCGGTGCCGG TCAGCTCGAT
  421 GCGATTCACC AGGGTATCGC CCTCGAACTT CACCTCGGCG CGCGACTTGT AGTTGCCGTC
  481 ATCCTCGAAG AAGATGGTGC GCTCCTGGAT GTAGCCCTCA GGCATGGCGC TCTTGAAGAA
  541 GTCGTGCTGC TTCATGTGAT CGGGGTAGCG TGAGAAGCAC TGCACGCCGT AGCTCAGGGT
  601 GGTCACCAGG GTGGGCCAGG GCACAGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT
  661 CAGCTTGCCG TANGTGNCAT CGCCCTCGCC CTCGCCGCTC ACGCTGAACT TGTGNCCATT
  721 CACATCGCCA TTCAGCTCGA TCAGGANGGG CACGATGCCN GTGAACAGCT CGGCGCCCTT
  781 GCTCACCATG ACCGGNGGAN CATGANGTTT GCGCTCGCGG ANNGNTTCNA CTTTGTCGAN
  841 GCTNGANNNN GNAGTTCCCC CACTTACTGN ATGNCCCGCT GCGGNATNCA GCNNNNCTTN
  901 CNCGTTGGNG GNNNTTCNAN NNNNTTNCNN GNCTNNCNNN NNNCNNNNNG NNCNTNNNNN
961 NNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: Neo R_Kan R
Sequence file: RDB14651_A7Cfd.seq check
>D04435A6_A7Cf_1_SV40pro_F_V2_B06_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GCTTTTGNNN NNNCGATCAA GAGANAGGNN GAGGNNCGTT
   61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  541 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
  601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
  721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGANCG
  781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
  841 TCTTGACNAN TTCTTCTGAN CGGGACTCTN GGGTNCNAAA TGACCNANCN ANCGACGCCN
  901 NACNNGCCNT CACNANNNTT CNANTNNNCC NNCNNCNTTC NNNNAANNNN NGGNNTNNNN
961 NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yamaguchi, A., Two novel heat-soluble protein families abundantly expressed in an anhydrobiotic tardigrade. PLoS One 7 (8): e44209 (2012). PMID 22937162. [link to RRC of NBRP]