Resource data sheet
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pEThT-SAHS2 (#RDB14647)

Expression vector of N-terminus 6xHis-tagged water bear (Ramazzottius varieornatus) Secretory Abundant Heat Soluble 2 (SAHS2) in E. coli.

Clone info. pET expression system vector of N-terminus 6xHis-tagged water bear (Ramazzottius varieornatus) Secretory Abundant Heat Soluble 2 (SAHS2) under the T7 promoter for expression recombinant protein in E. coli.
Comment Expression was confirmed by the depositor with SDS-PAGE and CD spectra.
Vector backbone pEThT (plasmid)
Selectable markers Amp^r
Gene/insert name Ramazzottius varieornatus SAHS2 cDNA
Reference of insert sequence AB650498 (DDBJ accession number)
Depositor|Developer Kunieda, Takekazu |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.（Yamaguchi, A et al. PLoS One, 7(8): e44209, 2012)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって開発者の指定する文献を引用する（Yamaguchi, A et al. PLoS One, 7(8): e44209, 2012)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14647 pEThT-SAHS2 Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pEThT-SAHS2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14647).

Reference section:

Yamaguchi, A., Tanaka, S., Yamaguchi, S., Kuwahara, H., Takamura, C., Imajoh-Ohmi, S., Horikawa, D.D., Toyoda, A., Katayama, T., Arakawa, K., Fujiyama, A., Kubo, T., Kunieda, T., Two novel heat-soluble protein families abundantly expressed in an anhydrobiotic tardigrade. PLoS One 7 (8): e44209 (2012). PMID 22937162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Yamaguchi, A., Two novel heat-soluble protein families abundantly expressed in an anhydrobiotic tardigrade. PLoS One 7 (8): e44209 (2012). PMID 22937162. [link to RRC of NBRP]