Expression vector of N-terminus 6xHis-tagged water bear (Ramazzottius varieornatus) Secretory Abundant Heat Soluble 1 (SAHS1) in E. coli.
|Clone info.||pET expression system vector of N-terminus 6xHis-tagged water bear (Ramazzottius varieornatus) Secretory Abundant Heat Soluble 1 (SAHS1) under the T7 promoter for expression recombinant protein in E. coli.
Expression was confirmed by the depositor with SDS-PAGE and CD spectra.
|Vector backbone||pEThT (plasmid)|
|Gene/insert name||Ramazzottius varieornatus SAHS1 cDNA|
|Reference of nucleotide sequence||AB650497 (DDBJ accession number)|
|Depositor||Kunieda, Takekazu ||
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
|Terms and conditions set forth by the DEPOSITOR||In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.（Yamaguchi, A et al. PLoS One, 7(8): e44209, 2012)|
(Japanese text [open/close])
利用者は、研究成果の公表にあたって開発者の指定する文献を引用する（Yamaguchi, A et al. PLoS One, 7(8): e44209, 2012)。
|Catalog #||Resource name||Shipping form||Fee (non-profit org.)|
This clone will be sequenced a portion and digested by restriction enzyme for examination.
|original||Yamaguchi, A., Two novel heat-soluble protein families abundantly expressed in an anhydrobiotic tardigrade. PLoS One 7 (8): e44209 (2012). PMID 22937162.|