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pcDNA4/chicken_ST3Gal5-His

Catalog number RDB14638
Resource name pcDNA4/chicken_ST3Gal5-His
Clone info. Expression vector of chicken beta-galactoside alpha-2,3-sialyltransferase 5 (ST3Gal5), C-terminal myc- and 6xHis-tag. cDNA fragment 128nt to 1231nt of NM_001001192.1 is cloned.
Primers were designed following the putative DNA sequence data of chicken ST3Gal5, NM_001001192. Expression was confirmed by the depositor with Western blotting and RT-qPCR. Known difference: A to G substitution at 433nt (Lys102, synonym).
Vector backbone pcDNA4/TO/Myc-HisA (Plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r (E. coli), Zeo^r (mammalian cells)
Gene/insert name Gallus gallus ST3GAL5 cDNA
Depositor Kaneoka, Hidenori |
 
Other clones in our bank Gallus gallus ST3GAL5 (KEGG orthology K03370) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Kidani, S et al. J Biosci Bioeng. 122 (4) : 379-384, 2016) RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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RDB14638 pcDNA4/chicken_ST3Gal5-His Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Kidani, S., Analyses of chicken sialyltransferases related to O-glycosylation. J. Biosci. Bioeng. 122 (4): 379-384 (2016). PMID 27150510.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Kidani, S., Analyses of chicken sialyltransferases related to O-glycosylation. J. Biosci. Bioeng. 122 (4): 379-384 (2016). PMID 27150510.

2019.07.19

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