Resource data sheet
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pcDNA4/chicken_ST3Gal1-His (#RDB14636)

Clone info. Expression vector of chicken beta-galactoside alpha-2,3-sialyltransferase 1 (ST3Gal1), C-terminal myc- and 6xHis-tag.
Comment Primers were designed following the putative DNA sequence data of chicken ST3Gal1, NM_205217. Expression was confirmed by the depositor with Western blotting and RT-qPCR.
Vector backbone pcDNA4/TO/Myc-HisA (Plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r (E. coli), Zeo^r (mammalian cells)
Gene/insert name Gallus gallus ST3GAL1 cDNA
Depositor|Developer Kaneoka, Hidenori |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.（Kidani, S et al. J Biosci Bioeng. 122 (4) : 379-384, 2016). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
提供案内 (日本国内) [open/close]

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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
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MTAに書く使用条件 １．利用者は、研究成果の公表にあたって開発者の指定する文献を引用する（Kidani, S et al. J Biosci Bioeng. 122 (4) : 379-384, 2016）。２．利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。３．営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14636 pcDNA4/chicken_ST3Gal1-His Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pcDNA4/chicken_ST3Gal1-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14636).

Reference section:

Kidani, S., Kaneoka, H., Okuzaki, Y., Asai, S., Kojima, Y., Nishijima, K., Iijima, S., Analyses of chicken sialyltransferases related to O-glycosylation. J. Biosci. Bioeng. 122 (4): 379-384 (2016). PMID 27150510. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

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References

Original, user report and related articles

original Kidani, S., Analyses of chicken sialyltransferases related to O-glycosylation. J. Biosci. Bioeng. 122 (4): 379-384 (2016). PMID 27150510. [link to RRC of NBRP]