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G-CaMP8

Genetically-encoded fluorescent calcium sensor cDNA, G-CaMP8.

Catalog number RDB14611
Resource name G-CaMP8
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. G-CaMP8 is high-sensitivity and fast-responsivity calcium sensor that reliably monitor neural spikes with larger fluorescence signals and higher temporal resolution.
Expression was confirmed by the depositor with calcium imaging.
Vector backbone pEGFP-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^R (E. coli), Neo^r (mammalian cells).
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence AKE44626.1 (DDBJ accession number)
Depositor Nakai, Junichi | Ohkura, Masamichi |
 
Other clones in our bank Rat Calm2 (KEGG orthology K02183) | Chickin MYLK (KEGG orthology K00907) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Ohkura, M. et al. PLos One 7 (12): e51286, 2012) and an acknowledgment to the DEPOSITOR are requested. The DEPOOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.
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Schedule A (GFP-A) [Link]
Remarks
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RDB14611 G-CaMP8 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Ohkura, M., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0067j (Japanese text)
Featured content Calcium Ion Sensor Clones (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14611_A8Brp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB14611_A8Bra.seq
>14611_14611_A8Br_2_CMV-Forward_A01_01_ABI08.ab1
    1 CAAGGGGTTC TATTAATAAA GCAGAGCTGG TTTAGTGGAA CCGTCAGATC CGCTAGCGCT
   61 ACCGGACTCA GATCTCGCCA CCATGGGTTC TCATCATCAT CATCATGGTA TGGCTAGCAT
  121 GACTGGTGGA CAGCAAATGG GTCGGGATCT GTACGACGAT GACGATAAGG ATCTCGCCAC
  181 CATGGTCGAC TCATCACGTC GTAAGTGGAA TAAGACAGGT CACGCAGTCA GAGCTATAGG
  241 TCGGCTGAGC TCACTCGAGA ACGTCTATAT CAAGGCCGAC AAGCAGAAGA ACGGCATCAA
  301 GGCGAACTTC AAGATCCGCC ACAACATCGA GGACGGCGGC GTGCAGCTCG CCTACCACTA
  361 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG
  421 CGTTCAGAAT AAACTTTCGA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA
  481 GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGGA GCTGTACAAG GGCGGTACCG
  541 GAGGGAGCAT GGTGAGCAAA GGGCGAGGAG CTGTTCACCG GGGGTGGTGC CCATCCTGGT
  601 CGAGCTGGAC GGCGACGTAA ACGGCCACAG TTCAGCGTGT CCGGCGAGGG TGAGGGCGAT
  661 GCCACCTACG GCAAGCTGAC CCTGAAGTCT TCTGCACACG GGCAAGCTGC CCGTGCCCTG
721 GCACCTCGTG ACACCCTGAC CTACGGCGTG CAAGTGCTTC AGACGCGCTA CCCCCGAGA
//
Primer: ME1290Rv RDB14611_A8Brb.seq
>14611_14611_A9DoS_ME1290Rv_G10_19_ABI24.ab1
    1 AATGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT
   61 AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCTAGA GTCGCGGCCG CTCACTTCGC
  121 TGTCATCATT TGTACAAACT CTTCGTAGTT TACCTGACCA TCCCCATCGA TGTCTGCTTC
  181 CCTGATCATT TCATCAACCT CTTCATCTGT TAACTTCTCT CCAAGGTTTG TCATCACGTG
  241 GCGAAGCTCT GCTGCACTGA TGTAGCCATT GCCATCCTTA TCAAACACAC GGAACGCTTC
  301 TCTAATTTCT TCTTCACTGT CTGTGTATTT CATTTTTCTT GCCATCATTG TCAGGAACTC
  361 AGGGAAGTCG ATTGTGCCAT CACCGTCGGC ATCTACTTCA TTGATCATGT CCTGCAGCTC
  421 TGCTTCTGTG GGGTTCTGCC CCAGAGACCG CAGCACCGTC CCCAGCTCCT TGGTTGTTAT
  481 TGTCCCATCC CCGTCCTTGT CAAATAGGGA GAAAGCCTCT TTAAATTCTG CGATCTGCTC
  541 TTCAGTCAGT TGGTCACGCG TGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC
  601 CTTGAAGTCG ATGCCCTTCA GCACGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC
  661 CTCGGCGCGG GTCTTGTAGT AGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGATGTA
  721 GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGCT
  781 GAAGCACTGC ACGCCGTAGG TCAGGGTGGT CACGAGGGTG GGCCAGGGCA CGGGCAGCTT
  841 GCCGGTGGTG CAGAAAGAAC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCACCCT
  901 CGCCCGGACA CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC CAGGGATGGG
  961 CACCACCCCG GGTGAACAGC TCCTCGCCTT GCTCACCATG CTCCCTCCGG GTACCGCCCT
 1021 TGTACAGCTC GTCCATGCCG AGAGTGATCC CCGGCGCGTC ACGACTCCAG CAGGATCATG
 1081 TGATCGCGCT TCTCGTGGGG TCTCTCGAGT AATCCTGGAC GGCTCAGGTT AGTTGAATTG
1141 TTCCAGGCAG CAAGCCACCG AGC
//
Primer: SV40pro_F_V2 RDB14611_A8Brc.seq
>14611_14611_A9DoS_SV40pro_F_V2_F10_16_ABI24.ab1
    1 GTACCAGCTT TTTTTGGGAG GCCTAGGCTT TTGCAAGATC GATCAAGAGA CAGGATGAGG
   61 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
  121 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
  181 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
  241 GAATGAACTG CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
  301 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
  361 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
  421 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
  481 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
  541 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG
  601 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
  661 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
  721 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
  781 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA
  841 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG
  901 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
  961 TTCGGAATCG TTTTCCGGGG ACGCCCGGCT GGATGATCCT CCCAGCGCGG GGAATCTCAT
 1021 GCTTGGAAGT TCTTTCGCCC ACCCCTAGGG GGAGGCTAAC TGAAACACGG AAGGGAGAAC
 1081 ATTACCGGAA AGAAATCCGC GGCTATTGAC CGGCATTAAA AGGACGGAAT AAAACGCCAC
1141 GGTGGTTGGG GTCGGTTTTG GGTTTCCTA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ohkura, M., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011.
reference Pastrana, E., Calcium sensors reach new heights. Nat. Methods 10 (9): 824 (2013). PMID 24143827.

2019.05.16

GNP_filter3_RDBDEP_html_190430.pl