Resource data sheet
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G-CaMP8

Genetically-encoded fluorescent calcium sensor cDNA, G-CaMP8.

Catalog number RDB14611
Resource name G-CaMP8
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. G-CaMP8 is high-sensitivity and fast-responsivity calcium sensor that reliably monitor neural spikes with larger fluorescence signals and higher temporal resolution.
Expression was confirmed by the depositor with calcium imaging.
Vector backbone pEGFP-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^R (E. coli), Neo^r (mammalian cells).
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence AKE44626.1 (DDBJ accession number)
Depositor Nakai, Junichi | Ohkura, Masamichi |
 
Other clones in our bank Rat Calm2 (KEGG orthology K02183) | Chickin MYLK (KEGG orthology K00907) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Ohkura, M. et al. PLos One 7 (12): e51286, 2012) and an acknowledgment to the DEPOSITOR are requested. The DEPOOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.
(Japanese text [open/close])
Remarks ((Additional form)) GFP-A
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14611 G-CaMP8 DNA solution

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14611_A7Bgp1_2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB14611_A7Bga.seq
>D04021A6_A7Bg_1_CMV-Forward_F10_16_ABI24.ab1
    1 TGGTTTCAAC TCCGCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGACT CAGATCTCGC
  121 CACCATGGGT TCTCATCATC ATCATCATGG TATGGCTAGC ATGACTGGTG GACAGCAAAT
  181 GGGTCGGGAT CTGTACGACG ATGACGATAA GGATCTCGCC ACCATGGTCG ACTCATCACG
  241 TCGTAAGTGG AATAAGACAG GTCACGCAGT CAGAGCTATA GGTCGGCTGA GCTCACTCGA
  301 GAACGTCTAT ATCAAGGCCG ACAAGCAGAA GAACGGCATC AAGGCGAACT TCAAGATCCG
  361 CCACAACATC GAGGACGGCG GCGTGCAGCT CGCCTACCAC TACCAGCAGA ACACCCCCAT
  421 CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCGTTCAGA ATAAACTTTC
  481 GAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG
  541 GATCACTCTC GGCATGGACG AGCTGTACAA GGGCGGTACC GGAGGGAGCA TGGTGAGCAA
  601 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA
  661 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG TGAGGGCGAT GCCACCTACG GCAAGCTGAC
  721 CCTGAAGTTC TTCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC
  781 CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT
  841 CTTCAAGTCC GCCATGCCCG AAAGCTACAT CCAGGAGCGC ACCATCTTCT TCAAGGACGA
  901 CGGCTACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT
  961 CGTGCTGAAG GGCATCGACT TCAAGGAGAC GGCAACATCC TGGGGGCACA AGCTGGAAGT
 1021 ACACACGCGT GACCAACTGA CTGGAGAGCA GATCGCAGAT TAAGAGCTTT TCCTCCTATT
1081 TGACAGGACG GAATGACAAT ACCAACAAGA AGCTGGGAGC ACGGTGTGCT TGC
//
Primer: SV40polyA-R RDB14611_A7Bgb.seq
>D04021A6_A7Bg_1_SV40polyA-R_F11_17_ABI24.ab1
    1 AAGAAATGGG AGTTTTTAAG CAGTAAAACC TCTACAAATG TGGTATGGCT GATTATGATC
   61 TAGAGTCGCG GCCGCTCACT TCGCTGTCAT CATTTGTACA AACTCTTCGT AGTTTACCTG
  121 ACCATCCCCA TCGATGTCTG CTTCCCTGAT CATTTCATCA ACCTCTTCAT CTGTTAACTT
  181 CTCTCCAAGG TTTGTCATCA CGTGGCGAAG CTCTGCTGCA CTGATGTAGC CATTGCCATC
  241 CTTATCAAAC ACACGGAACG CTTCTCTAAT TTCTTCTTCA CTGTCTGTGT ATTTCATTTT
  301 TCTTGCCATC ATTGTCAGGA ACTCAGGGAA GTCGATTGTG CCATCACCGT CGGCATCTAC
  361 TTCATTGATC ATGTCCTGCA GCTCTGCTTC TGTGGGGTTC TGCCCCAGAG ACCGCAGCAC
  421 CGTCCCCAGC TCCTTGGTTG TTATTGTCCC ATCCCCGTCC TTGTCAAATA GGGAGAAAGC
  481 CTCTTTAAAT TCTGCGATCT GCTCTTCAGT CAGTTGGTCA CGCGTGTTGT ACTCCAGCTT
  541 GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCACGA TGCGGTTCAC
  601 CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTAGCCGT CGTCCTTGAA
  661 GAAGATGGTG CGCTCCTGGA TGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG
  721 CTTCATGTGG TCGGGGTAGC GGCTGAAGCA CTGCACGCCG TAGGTCAGGG TGGTCACGAG
  781 GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGAAG AACTTCAGGG TCAGCTTGCC
  841 GTAGTGGCAT CGCCCTCACC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG
  901 TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG
  961 CTCCCTCCGG GTACCGCCCT TGTACAGCTC GTCCATGCCG AGAGTGATCC CCGGCGGCGG
 1021 TCACGACTCC AGCAGACATG TGATCGCGCT TTCTCGTGGG GTCTTTCGAA GTTTATTTCT
 1081 GACGCCTCAG TAATTGATGG TCGGGCAGCA GCACGGTCGT CGTGATGGGG TGATTCTGCC
1141 TGTTATTGTT AGCGAGCTGC ACGTCGC
//
Primer: SV40pro_F_V2 RDB14611_A7Bgc.seq
>D04021A6_A7Bg_1_SV40pro_F_V2_F12_18_ABI24.ab1
    1 GGCAGAACTT TTTGGAGCTA GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG
   61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
  601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC
  901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG
  961 GAATCGTTTT CCCGGGACGC CCGGCTGGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG
 1021 GAGTTCTTCG CCACCCTAGG GGGAGCCTAC TGAAACACGG GATGAGACAA TACTGCACGA
 1081 CCCGCGCTAT GACGGCATAA AGACCGATTA ACGGCACGGT GTTGGGATCG GTTTGGTTCC
1141 AATAAAACGT CG
//

Please visit Sequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content Dnaconda's recommendation EXR0067j (Japanese text)
Featured content Calcium Ion Sensor Clones (English text)

References

original Ohkura, M., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011.
reference Pastrana, E., Calcium sensors reach new heights. Nat. Methods 10 (9): 824 (2013). PMID 24143827.

2018.05.18

GNP_filter3_RDBDEP_html_171214.pl