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G-CaMP8

Genetically-encoded fluorescent calcium sensor cDNA, G-CaMP8.

Catalog number RDB14611
Resource name G-CaMP8
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. G-CaMP8 is high-sensitivity and fast-responsivity calcium sensor that reliably monitor neural spikes with larger fluorescence signals and higher temporal resolution.
Comment Expression was confirmed by the depositor with calcium imaging.
Vector backbone pEGFP-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^R (E. coli), Neo^r (mammalian cells).
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of insert sequence AKE44626.1 (DDBJ accession number)
Depositor|Developer Nakai, Junichi | Ohkura, Masamichi |
Other clones in our bank

External Database
Rat Calm2 Chickin MLCK Aequorea victoria GFP

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Ohkura, M. et al. PLos One 7 (12): e51286, 2012) and an acknowledgment to the DEPOSITOR are requested. The DEPOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        提供案内 (日本国内) [open/close]

        提供条件 研究成果の公表にあたって寄託者の指定する文献(Ohkura, M. et al. PLos One 7 (12): e51286, 2012)を引用し、謝辞の表明を必要とする。寄託者は、利用の状況及び成果等について利用者に報告を求めることができ、利用者は誠実に寄託者の求めに対して回答することとする。当該リソースの営利目的での利用(営利団体による利用、非営利団体が営利団体から直接・間接を問わず資金等を受けて実施する研究、非営利団体と営利団体との共同研究、非営利団体による特許取得・ライセンス契約等の行為等)の希望については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB14611 G-CaMP8 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Ohkura, M., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Dnaconda's recommendation EXR0067j (Japanese text)
        Featured content Calcium Ion Sensor Clones (English text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB14611_A7Bgp1-3.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV_Forward
        Region: CMV pro,5xHis,T7 tag,Xpress tag,insert 5'
        Sequence file: RDB14611_A7Bga.seq check
        >D04021A6_A7Bg_1_CMV-Forward_F10_16_ABI24.ab1
            1 TGGTTTCAAC TCCGCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
           61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGACT CAGATCTCGC
          121 CACCATGGGT TCTCATCATC ATCATCATGG TATGGCTAGC ATGACTGGTG GACAGCAAAT
          181 GGGTCGGGAT CTGTACGACG ATGACGATAA GGATCTCGCC ACCATGGTCG ACTCATCACG
          241 TCGTAAGTGG AATAAGACAG GTCACGCAGT CAGAGCTATA GGTCGGCTGA GCTCACTCGA
          301 GAACGTCTAT ATCAAGGCCG ACAAGCAGAA GAACGGCATC AAGGCGAACT TCAAGATCCG
          361 CCACAACATC GAGGACGGCG GCGTGCAGCT CGCCTACCAC TACCAGCAGA ACACCCCCAT
          421 CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCGTTCAGA ATAAACTTTC
          481 GAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG
          541 GATCACTCTC GGCATGGACG AGCTGTACAA GGGCGGTACC GGAGGGAGCA TGGTGAGCAA
          601 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA
          661 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG TGAGGGCGAT GCCACCTACG GCAAGCTGAC
          721 CCTGAAGTTC TTCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC
          781 CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT
          841 CTTCAAGTCC GCCATGCCCG AAAGCTACAT CCAGGAGCGC ACCATCTTCT TCAAGGACGA
          901 CGGCTACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT
          961 CGTGCTGAAG GGCATCGACT TCAAGGAGAC GGCAACATCC TGGGGGCACA AGCTGGAAGT
         1021 ACACACGCGT GACCAACTGA CTGGAGAGCA GATCGCAGAT TAAGAGCTTT TCCTCCTATT
        1081 TGACAGGACG GAATGACAAT ACCAACAAGA AGCTGGGAGC ACGGTGTGCT TGC
        //
        Primer: SV40polyA-R
        Region: insert 3'
        Sequence file: RDB14611_A7Bgb.seq check
        >D04021A6_A7Bg_1_SV40polyA-R_F11_17_ABI24.ab1
            1 AAGAAATGGG AGTTTTTAAG CAGTAAAACC TCTACAAATG TGGTATGGCT GATTATGATC
           61 TAGAGTCGCG GCCGCTCACT TCGCTGTCAT CATTTGTACA AACTCTTCGT AGTTTACCTG
          121 ACCATCCCCA TCGATGTCTG CTTCCCTGAT CATTTCATCA ACCTCTTCAT CTGTTAACTT
          181 CTCTCCAAGG TTTGTCATCA CGTGGCGAAG CTCTGCTGCA CTGATGTAGC CATTGCCATC
          241 CTTATCAAAC ACACGGAACG CTTCTCTAAT TTCTTCTTCA CTGTCTGTGT ATTTCATTTT
          301 TCTTGCCATC ATTGTCAGGA ACTCAGGGAA GTCGATTGTG CCATCACCGT CGGCATCTAC
          361 TTCATTGATC ATGTCCTGCA GCTCTGCTTC TGTGGGGTTC TGCCCCAGAG ACCGCAGCAC
          421 CGTCCCCAGC TCCTTGGTTG TTATTGTCCC ATCCCCGTCC TTGTCAAATA GGGAGAAAGC
          481 CTCTTTAAAT TCTGCGATCT GCTCTTCAGT CAGTTGGTCA CGCGTGTTGT ACTCCAGCTT
          541 GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCACGA TGCGGTTCAC
          601 CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTAGCCGT CGTCCTTGAA
          661 GAAGATGGTG CGCTCCTGGA TGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG
          721 CTTCATGTGG TCGGGGTAGC GGCTGAAGCA CTGCACGCCG TAGGTCAGGG TGGTCACGAG
          781 GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGAAG AACTTCAGGG TCAGCTTGCC
          841 GTAGTGGCAT CGCCCTCACC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG
          901 TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG
          961 CTCCCTCCGG GTACCGCCCT TGTACAGCTC GTCCATGCCG AGAGTGATCC CCGGCGGCGG
         1021 TCACGACTCC AGCAGACATG TGATCGCGCT TTCTCGTGGG GTCTTTCGAA GTTTATTTCT
         1081 GACGCCTCAG TAATTGATGG TCGGGCAGCA GCACGGTCGT CGTGATGGGG TGATTCTGCC
        1141 TGTTATTGTT AGCGAGCTGC ACGTCGC
        //
        Primer: SV40pro_F_V2
        Region: NeoR_KanR
        Sequence file: RDB14611_A7Bgc.seq check
        >D04021A6_A7Bg_1_SV40pro_F_V2_F12_18_ABI24.ab1
            1 GGCAGAACTT TTTGGAGCTA GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG
           61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
          121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
          181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
          241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
          301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
          361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
          421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
          481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
          541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
          601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
          661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
          721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
          781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
          841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC
          901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG
          961 GAATCGTTTT CCCGGGACGC CCGGCTGGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG
         1021 GAGTTCTTCG CCACCCTAGG GGGAGCCTAC TGAAACACGG GATGAGACAA TACTGCACGA
         1081 CCCGCGCTAT GACGGCATAA AGACCGATTA ACGGCACGGT GTTGGGATCG GTTTGGTTCC
        1141 AATAAAACGT CG
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Ohkura, M., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011.
        review Pastrana, E., Calcium sensors reach new heights. Nat. Methods 10 (9): 824 (2013). PMID 24143827.

        2022.07.13

        GNP_filter3_RDBDEP_html_220713.pl