Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

G-CaMP7

Genetically-encoded fluorescent calcium sensor cDNA, G-CaMP7.

Catalog number RDB14610
Resource name G-CaMP7
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. G-CaMP7 is high-sensitivity and fast-responsivity calcium sensor that reliably monitor neural spikes with larger fluorescence signals and higher temporal resolution.
Comment Expression was confirmed by the depositor with calcium imaging.
Vector backbone pEGFP-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^R (E. coli), Neo^r (mammalian cells).
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of insert sequence KP230402.1 (DDBJ accession number)
Depositor|Developer Nakai, Junichi | Ohkura, Masamichi |
Other clones in our bank

External Database
Rat Calm2 Chickin MLCK Aequorea victoria GFP

        Reference sequence
          AKE44625.1

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Ohkura, M. et al. PLos One 7 (12): e51286, 2012) and an acknowledgment to the DEPOSITOR are requested. The DEPOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        提供案内 (日本国内) [open/close]

        提供条件 研究成果の公表にあたって寄託者の指定する文献(Ohkura, M. et al. PLos One 7 (12): e51286, 2012)を引用し、謝辞の表明を必要とする。寄託者は、利用の状況及び成果等について利用者に報告を求めることができ、利用者は誠実に寄託者の求めに対して回答することとする。当該リソースの営利目的での利用(営利団体による利用、非営利団体が営利団体から直接・間接を問わず資金等を受けて実施する研究、非営利団体と営利団体との共同研究、非営利団体による特許取得・ライセンス契約等の行為等)の希望については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB14610 G-CaMP7 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Ohkura, M., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Dnaconda's recommendation EXR0067j (Japanese text)
        Featured content Calcium Ion Sensor Clones (English text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB14610_A7Bgp1-2.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV-Forward
        Sequence file: RDB14610_A7Bga.seq check
        >D04021A5_A7Bg_1_CMV-Forward_E10_13_ABI24.ab1
            1 GTCGGGACCC AACTCCGGCC CCATTGACGG CAAATGGGGC GGTTAGGCGT GTACGGTGGG
           61 AGGTCTATAT AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGACTC
          121 AGATCTCGCC ACCATGCGGG GTTCTCATCA TCATCATCAT GGTATGGCTA GCATGACTGG
          181 TGGACAGCAA ATGGGTCGGG ATCTGTACGA CGATGACGAT AAGGATCTCG CCACCATGGT
          241 CGACTCATCA CGTCGTAAGT GGAATAAGAC AGGTCACGCA GTCAGAGCTA TAGGTCGGCT
          301 GAGCTCACTC GAGAACGTCT ATATCAAGGC CGACAAGCAG AAGAACGGCA TCAAGGCGAA
          361 CTTCAAGATC CGCCACAACA TCGAGGACGG CGGCGTGCAG CTCGCCTACC ACTACCAGCA
          421 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCGTTCA
          481 GAATAAACTT TCGAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT
          541 GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGGGCGGTA CCGGAGGGAG
          601 CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA
          661 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGTGAGGGCG ATGCCACCTA
          721 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
          781 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA
          841 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC ATCCAGGAGC GCACCATCTT
          901 CTTCAAAGGA CGACGGGCTA CTACAAGACC CGCGCCGAGG GTGAAGTTTC GAGGGCGACA
          961 CCCCTGGGTG AACCCGCATC GTTGCTGAAA GGGCATCGAC TTTCAAGGAG GACGGGCAAC
         1021 ATCCCTGGGG GCCACAGCTG GGAGTACCAA CAACGGCGGT GACCCAACCT GACTGGAGAG
         1081 CCAGAATCCG CCAGGAATTT AAAAGAGGCC TTTCCTCCCC TATTTGGACA AGGAACGGGG
        1141 ATTGGGACCA ATAAACACCT AGGAAGGACC CTGTGCGGGG GACACCGGGG CGTGG
        //
        Primer: SV40polyA-R
        Sequence file: RDB14610_A7Bgb.seq check
        >D04021A5_A7Bg_1_SV40polyA-R_E11_14_ABI24.ab1
            1 AGAAAGTTGG CAGTTTTTAA GCAGTAAACC TCTACAAATG TGGTATGGCT GATTATGATC
           61 TAGAGTCGCG GCCGCTCACT TCGCTGTCAT CATTTGTACA AACTCTTCGT AGTTTACCTG
          121 ACCATCCCCA TCGATGTCTG CTTCCCTGAT CATTTCATCA ACCTCTTCAT CTGTTAACTT
          181 CTCTCCAAGG TTTGTCATCA CGTGGCGAAG CTCTGCTGCA CTGATGTAGC CATTGCCATC
          241 CTTATCAAAC ACACGGAACG CTTCTCTAAT TTCTTCTTCA CTGTCTGTGT ATTTCATTTT
          301 TCTTGCCATC ATTGTCAGGA ACTCAGGGAA GTCGATTGTG CCATCACCGT CGGCATCTAC
          361 TTCATTGATC ATGTCCTGCA GCTCTGCTTC TGTGGGGTTC TGCCCCAGAG ACCGCAGCAC
          421 CGTCCCCAGC TCCTTGGTTG TTATTGTCCC ATCCCCGTCC TTGTCAAATA GGGAGAAAGC
          481 CTCTTTAAAT TCTGCGATCT GCTCTTCAGT CAGTTGGTCA CGCGTGTTGT ACTCCAGCTT
          541 GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCACGA TGCGGTTCAC
          601 CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTAGCCGT CGTCCTTGAA
          661 GAAGATGGTG CGCTCCTGGA TGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG
          721 CTTCATGTGG TCGGGGTAGC GGCTGAAGCA CTGCACGCCG TAGTCAGGGT GGTCACGAGG
          781 GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG
          841 TACGTGGCAT CGCCCTCACC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG
          901 TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG
          961 CTCCCTCCGG GTACCGCCCT TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGGCGGCGG
         1021 GTCACGAACT CCAGCAGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTC GAAAGTTTAT
         1081 TCTGACGCTC AGGTAGTGAT GGTCGGGCAG CAGCACGGGG TCGTTCGTGA ATGGAGGTGT
        1141 TCCTGGCTGC TAGTGGTAGG GCGGAGCTTG CAACGACCGT TCCGGT
        //
        Primer: SV40pro_F_V2
        Sequence file: RDB14610_A7Bgc.seq check
        >D04021A5_A7Bg_1_SV40pro_F_V2_E12_15_ABI24.ab1
            1 ATAAAACCTT TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA
           61 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
          121 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
          181 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
          241 AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
          301 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
          361 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
          421 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
          481 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
          541 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC
          601 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
          661 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC
          721 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT
          781 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
          841 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA
          901 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT
          961 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCCTCC AGCGCGGGGA TCTCATGCTG
         1021 GAGTTTCTTT CGCCACCCTA GGGGGAGGCT ACTGAAACAC GGAAAGAGAC ATACCGGAGG
         1081 ACCCGCGCTA ATGACGGCAA TAAAGACCGA TTAAACCGCC ACGGATGTTG GGGTTCGATT
        1141 TTGTTCAATT AC
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Ohkura, M., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011.
        reference Monai, H., Calcium imaging reveals glial involvement in transcranial direct current stimulation-induced plasticity in mouse brain. Nat. Commun. 7: 11100 (2016). PMID 27000523.
        reference Sato, M., Generation and Imaging of Transgenic Mice that Express G-CaMP7 under a Tetracycline Response Element. PLoS One 10 (5): e0125354 (2015). PMID 25946002.
        review Pastrana, E., Calcium sensors reach new heights. Nat. Methods 10 (9): 824 (2013). PMID 24143827.

        2022.07.13

        GNP_filter3_RDBDEP_html_220713.pl