Resource data sheet
Prev.

G-CaMP7

Genetically-encoded fluorescent calcium sensor cDNA, G-CaMP7.

Catalog number RDB14610
Resource name G-CaMP7
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. G-CaMP7 is high-sensitivity and fast-responsivity calcium sensor that reliably monitor neural spikes with larger fluorescence signals and higher temporal resolution.
Expression was confirmed by the depositor with calcium imaging.
Vector backbone pEGFP-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^R (E. coli), Neo^r (mammalian cells).
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence AKE44625.1 (DDBJ accession number)
Depositor Nakai, Junichi | Ohkura, Masamichi |
 
Other clones in our bank Rat Calm2 (KEGG orthology K02183) | Chickin MYLK (KEGG orthology K00907) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Ohkura, M. et al. PLos One 7 (12): e51286, 2012) and an acknowledgment to the DEPOSITOR are requested. The DEPOOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.
(Japanese text [open/close])
Remarks ((Additional form)) GFP-A
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14610 G-CaMP7 DNA solution

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14610_A7Bgp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB14610_A7Bga.seq
>D04021A5_A7Bg_1_CMV-Forward_E10_13_ABI24.ab1
    1 GTCGGGACCC AACTCCGGCC CCATTGACGG CAAATGGGGC GGTTAGGCGT GTACGGTGGG
   61 AGGTCTATAT AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGACTC
  121 AGATCTCGCC ACCATGCGGG GTTCTCATCA TCATCATCAT GGTATGGCTA GCATGACTGG
  181 TGGACAGCAA ATGGGTCGGG ATCTGTACGA CGATGACGAT AAGGATCTCG CCACCATGGT
  241 CGACTCATCA CGTCGTAAGT GGAATAAGAC AGGTCACGCA GTCAGAGCTA TAGGTCGGCT
  301 GAGCTCACTC GAGAACGTCT ATATCAAGGC CGACAAGCAG AAGAACGGCA TCAAGGCGAA
  361 CTTCAAGATC CGCCACAACA TCGAGGACGG CGGCGTGCAG CTCGCCTACC ACTACCAGCA
  421 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCGTTCA
  481 GAATAAACTT TCGAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT
  541 GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGGGCGGTA CCGGAGGGAG
  601 CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA
  661 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGTGAGGGCG ATGCCACCTA
  721 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
  781 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA
  841 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC ATCCAGGAGC GCACCATCTT
  901 CTTCAAAGGA CGACGGGCTA CTACAAGACC CGCGCCGAGG GTGAAGTTTC GAGGGCGACA
  961 CCCCTGGGTG AACCCGCATC GTTGCTGAAA GGGCATCGAC TTTCAAGGAG GACGGGCAAC
 1021 ATCCCTGGGG GCCACAGCTG GGAGTACCAA CAACGGCGGT GACCCAACCT GACTGGAGAG
 1081 CCAGAATCCG CCAGGAATTT AAAAGAGGCC TTTCCTCCCC TATTTGGACA AGGAACGGGG
1141 ATTGGGACCA ATAAACACCT AGGAAGGACC CTGTGCGGGG GACACCGGGG CGTGG
//
Primer: SV40polyA-R RDB14610_A7Bgb.seq
>D04021A5_A7Bg_1_SV40polyA-R_E11_14_ABI24.ab1
    1 AGAAAGTTGG CAGTTTTTAA GCAGTAAACC TCTACAAATG TGGTATGGCT GATTATGATC
   61 TAGAGTCGCG GCCGCTCACT TCGCTGTCAT CATTTGTACA AACTCTTCGT AGTTTACCTG
  121 ACCATCCCCA TCGATGTCTG CTTCCCTGAT CATTTCATCA ACCTCTTCAT CTGTTAACTT
  181 CTCTCCAAGG TTTGTCATCA CGTGGCGAAG CTCTGCTGCA CTGATGTAGC CATTGCCATC
  241 CTTATCAAAC ACACGGAACG CTTCTCTAAT TTCTTCTTCA CTGTCTGTGT ATTTCATTTT
  301 TCTTGCCATC ATTGTCAGGA ACTCAGGGAA GTCGATTGTG CCATCACCGT CGGCATCTAC
  361 TTCATTGATC ATGTCCTGCA GCTCTGCTTC TGTGGGGTTC TGCCCCAGAG ACCGCAGCAC
  421 CGTCCCCAGC TCCTTGGTTG TTATTGTCCC ATCCCCGTCC TTGTCAAATA GGGAGAAAGC
  481 CTCTTTAAAT TCTGCGATCT GCTCTTCAGT CAGTTGGTCA CGCGTGTTGT ACTCCAGCTT
  541 GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCACGA TGCGGTTCAC
  601 CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTAGCCGT CGTCCTTGAA
  661 GAAGATGGTG CGCTCCTGGA TGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG
  721 CTTCATGTGG TCGGGGTAGC GGCTGAAGCA CTGCACGCCG TAGTCAGGGT GGTCACGAGG
  781 GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG
  841 TACGTGGCAT CGCCCTCACC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG
  901 TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG
  961 CTCCCTCCGG GTACCGCCCT TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGGCGGCGG
 1021 GTCACGAACT CCAGCAGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTC GAAAGTTTAT
 1081 TCTGACGCTC AGGTAGTGAT GGTCGGGCAG CAGCACGGGG TCGTTCGTGA ATGGAGGTGT
1141 TCCTGGCTGC TAGTGGTAGG GCGGAGCTTG CAACGACCGT TCCGGT
//
Primer: SV40pro_F_V2 RDB14610_A7Bgc.seq
>D04021A5_A7Bg_1_SV40pro_F_V2_E12_15_ABI24.ab1
    1 ATAAAACCTT TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA
   61 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
  121 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
  181 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
  241 AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
  301 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
  361 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
  421 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
  481 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
  541 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC
  601 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
  661 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC
  721 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT
  781 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
  841 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA
  901 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT
  961 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCCTCC AGCGCGGGGA TCTCATGCTG
 1021 GAGTTTCTTT CGCCACCCTA GGGGGAGGCT ACTGAAACAC GGAAAGAGAC ATACCGGAGG
 1081 ACCCGCGCTA ATGACGGCAA TAAAGACCGA TTAAACCGCC ACGGATGTTG GGGTTCGATT
1141 TTGTTCAATT AC
//

Please visit Sequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content Dnaconda's recommendation EXR0067j (Japanese text)
Featured content Calcium Ion Sensor Clones (English text)

References

original Ohkura, M., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011.
reference Monai, H., Calcium imaging reveals glial involvement in transcranial direct current stimulation-induced plasticity in mouse brain. Nat. Commun. 7: 11100 (2016). PMID 27000523.
reference Sato, M., Generation and Imaging of Transgenic Mice that Express G-CaMP7 under a Tetracycline Response Element. PLoS One 10 (5): e0125354 (2015). PMID 25946002.
reference Pastrana, E., Calcium sensors reach new heights. Nat. Methods 10 (9): 824 (2013). PMID 24143827.

2018.05.18

GNP_filter3_RDBDEP_html_171214.pl