Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

G-CaMP6

Genetically-encoded fluorescent calcium sensor cDNA, G-CaMP6.

Catalog number RDB14609
Resource name G-CaMP6
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. G-CaMP6 is high-sensitivity and fast-responsivity calcium sensor that reliably monitor neural spikes with larger fluorescence signals and higher temporal resolution.
Comment Expression was confirmed by the depositor with calcium imaging.
Vector backbone pEGFP-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^R (E. coli), Neo^r (mammalian cells).
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of insert sequence AKE44624.1 (DDBJ accession number)
Depositor|Developer Nakai, Junichi | Ohkura, Masamichi |
Other clones in our bank

External Database
Rat Calm2 Chickin MLCK Aequorea victoria GFP

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Ohkura, M. et al. PLos One 7 (12): e51286, 2012) and an acknowledgment to the DEPOSITOR are requested. The DEPOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        提供案内 (日本国内) [open/close]

        提供条件 研究成果の公表にあたって寄託者の指定する文献(Ohkura, M. et al. PLos One 7 (12): e51286, 2012)を引用し、謝辞の表明を必要とする。寄託者は、利用の状況及び成果等について利用者に報告を求めることができ、利用者は誠実に寄託者の求めに対して回答することとする。当該リソースの営利目的での利用(営利団体による利用、非営利団体が営利団体から直接・間接を問わず資金等を受けて実施する研究、非営利団体と営利団体との共同研究、非営利団体による特許取得・ライセンス契約等の行為等)の希望については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB14609 G-CaMP6 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Ohkura, M., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Dnaconda's recommendation EXR0067j (Japanese text)
        Featured content Calcium Ion Sensor Clones (English text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB14609_A7Bgp1-4.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV_Forward
        Region: CMV pro, 6xHis,T7 tag,Xpress tag, insert 5'
        Sequence file: RDB14609_A7Bga.seq check
        >D04021A4_A7Bg_1_CMV-Forward_D10_10_ABI24.ab1
            1 AGGTATAAAA ACTCCCGCCC CAATTGACGG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG
           61 TCTATATAAG CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA
          121 TCTCGCCACC ATGCGGGGTT CTCATCATCA TCATCATCAT GGTATGGCTA GCATGACTGG
          181 TGGACAGCAA ATGGGTCGGG ATCTGTACGA CGATGACGAT AAGGATCTCG CCACCATGGT
          241 CGACTCATCA CGTCGTAAGT GGAATAAGAC AGGTCACGCA GTCAGAGCTA TAGGTCGGCT
          301 GAGCTCACTC GAGAACGTCT ATATCAAGGC CGACAAGCAG AAGAACGGCA TCAAGGCGAA
          361 CTTCAAGATC CGCCACAACA TCGAGGACGG CGGCGTGCAG CTCGCCTACC ACTACCAGCA
          421 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCGTGCA
          481 GTCCAAACTT TCGAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT
          541 GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGGGCGGTA CCGGAGGGAG
          601 CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA
          661 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGTGAGGGCG ATGCCACCTA
          721 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
          781 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA
          841 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC ATCCAGGAGC GCACCATCTT
          901 CTTCAAGGAC GACGGCTACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT
          961 GGTTGAACCG CATCGTGCTG AAGGGCATCG ACTTTCAAGG AGGACGCAAC ATCCTGGGGC
         1021 ACAAGCTGGA GTACAACACG CCGTGACCAA CTGACTGAAG AGCAGATCGC AAGAATTAAG
         1081 AGCTTTCTCC CCTATTTGAC AAGAACGAAT GGGACAATTA CAACCAAGAC TTGGGAAACG
        1141 GTGCCTGTGC CGGATCTC
        //
        Primer: ME-1290RV
        Region: insert 3'
        Sequence file: RDB14609_A7Bgd.seq check
        >14609_14609_A9EmF_ME-1290Rv_C08_08_ABI24.ab1
            1 AGCAATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA
           61 AAACCTCTAC AAATGTGGTA TGGCTGATTA TGATCTAGAG TCGCGGCCGC TCACTTCGCT
          121 GTCATCATTT GTACAAACTC TTCGTAGTTT ACCTGACCAT CCCCATCGAT GTCTGCTTCC
          181 CTGATCATTT CATCAACCTC TTCATCTGTT AACTTCTCTC CAAGGTTTGT CATCACGTGG
          241 CGAAGCTCTG CTGCACTGAT GTAGCCATTG CCATCCTTAT CAAACACACG GAACGCTTCT
          301 CTAATTTCTT CTTCACTGTC TGTGTATTTC ATTTTTCTTG CCATCATTGT CAGGAACTCA
          361 GGGAAGTCGA TTGTGCCATC ACCGTCGGCA TCTACTTCAT TGATCATGTC CTGCAGCTCT
          421 GCTTCTGTGG GGTTCTGCCC CAGAGACCGC AGCACCGTCC CCAGCTCCTT GGTTGTTATT
          481 GTCCCATCCC CGTCCTTGTC AAATAGGGAG AAAGCCTCTT TAAATTCTGC GATCTGCTCT
          541 TCAGTCAGTT GGTCACGCGT GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC
          601 TTGAAGTCGA TGCCCTTCAG CACGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC
          661 TCGGCGCGGG TCTTGTAGTA GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC CTGGATGTAG
          721 CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG GTAGCGGCTG
          781 AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG
          841 CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTAGT GGCATCGCCC TCACCCTCGC
          901 CGGACACGCT GAAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG GATGGGCACC
          961 ACCCCGGTGA CAGCTCTCGC CCTTTGCTCA CATGCTCCCT CGGTACGCCC TGTACAGGCT
         1021 CGTTCATGGC GAGAGTGAAT CCTGCGGCGT TCACGGACTC AGCAGACCAT GTGATCGCGC
        1081 TCTCGATAGG ATCTATA
        //
        Primer: SV40pro_F_V2
        Region: SV40 pro_ori,NeoR_KanR
        Sequence file: RDB14609_A7Bgc.seq check
        >D04021A4_A7Bg_1_SV40pro_F_V2_D12_12_ABI24.ab1
            1 AACAAACTTT TTTGGAGGCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG
           61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
          121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
          181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
          241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
          301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
          361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
          421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
          481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
          541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
          601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
          661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
          721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
          781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
          841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC
          901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG
          961 GAATCGTTTT CCCGGGACGC CGGCTGGATG ATCCCTCCAG CGCGGGGATC TCATGCTGGG
         1021 AGTTCTTCGC CCACCCTAGG GGGAGGCTAA CTGAAACACG GAAGGAGACA TACCCGGAGA
         1081 CCCGCGCTAT GACGGGCAAT AAAGACGGAA TTAAACGGCA CGGATGATGG GATCGTTTTG
        1141 TTCCATATAA AACCGCCCGA G
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Ohkura, M., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011.
        review Pastrana, E., Calcium sensors reach new heights. Nat. Methods 10 (9): 824 (2013). PMID 24143827.

        2022.07.13

        GNP_filter3_RDBDEP_html_220713.pl