Resource data sheet
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G-CaMP6

Genetically-encoded fluorescent calcium sensor cDNA, G-CaMP6.

Catalog number RDB14609
Resource name G-CaMP6
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. G-CaMP6 is high-sensitivity and fast-responsivity calcium sensor that reliably monitor neural spikes with larger fluorescence signals and higher temporal resolution.
Expression was confirmed by the depositor with calcium imaging.
Vector backbone pEGFP-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^R (E. coli), Neo^r (mammalian cells).
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence AKE44624.1 (DDBJ accession number)
Depositor Nakai, Junichi | Ohkura, Masamichi |
 
Other clones in our bank Rat Calm2 (KEGG orthology K02183) | Chickin MYLK (KEGG orthology K00907) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Ohkura, M. et al. PLos One 7 (12): e51286, 2012) and an acknowledgment to the DEPOSITOR are requested. The DEPOOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.
(Japanese text [open/close])
Remarks ((Additional form)) GFP-A
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14609 G-CaMP6 DNA solution

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14609_A7Bgp1_2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB14609_A7Bga.seq
>D04021A4_A7Bg_1_CMV-Forward_D10_10_ABI24.ab1
    1 AGGTATAAAA ACTCCCGCCC CAATTGACGG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG
   61 TCTATATAAG CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA
  121 TCTCGCCACC ATGCGGGGTT CTCATCATCA TCATCATCAT GGTATGGCTA GCATGACTGG
  181 TGGACAGCAA ATGGGTCGGG ATCTGTACGA CGATGACGAT AAGGATCTCG CCACCATGGT
  241 CGACTCATCA CGTCGTAAGT GGAATAAGAC AGGTCACGCA GTCAGAGCTA TAGGTCGGCT
  301 GAGCTCACTC GAGAACGTCT ATATCAAGGC CGACAAGCAG AAGAACGGCA TCAAGGCGAA
  361 CTTCAAGATC CGCCACAACA TCGAGGACGG CGGCGTGCAG CTCGCCTACC ACTACCAGCA
  421 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCGTGCA
  481 GTCCAAACTT TCGAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT
  541 GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGGGCGGTA CCGGAGGGAG
  601 CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA
  661 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGTGAGGGCG ATGCCACCTA
  721 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
  781 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA
  841 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC ATCCAGGAGC GCACCATCTT
  901 CTTCAAGGAC GACGGCTACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT
  961 GGTTGAACCG CATCGTGCTG AAGGGCATCG ACTTTCAAGG AGGACGCAAC ATCCTGGGGC
 1021 ACAAGCTGGA GTACAACACG CCGTGACCAA CTGACTGAAG AGCAGATCGC AAGAATTAAG
 1081 AGCTTTCTCC CCTATTTGAC AAGAACGAAT GGGACAATTA CAACCAAGAC TTGGGAAACG
1141 GTGCCTGTGC CGGATCTC
//
Primer: SV40polyA-R RDB14609_A7Bgb.seq
>D04021A4_A7Bg_1_SV40polyA-R_D11_11_ABI24.ab1
    1 AGGAAATTGG AGTTTTTAAG CAGTAAACCT CTACAAATGT GGTATGGCTG ATTATGATCT
   61 AGAGTCGCGG CCGCTCACTT CGCTGTCATC ATTTGTACAA ACTCTTCGTA GTTTACCTGA
  121 CCATCCCCAT CGATGTCTGC TTCCCTGATC ATTTCATCAA CCTCTTCATC TGTTAACTTC
  181 TCTCCAAGGT TTGTCATCAC GTGGCGAAGC TCTGCTGCAC TGATGTAGCC ATTGCCATCC
  241 TTATCAAACA CACGGAACGC TTCTCTAATT TCTTCTTCAC TGTCTGTGTA TTTCATTTTT
  301 CTTGCCATCA TTGTCAGGAA CTCAGGGAAG TCGATTGTGC CATCACCGTC GGCATCTACT
  361 TCATTGATCA TGTCCTGCAG CTCTGCTTCT GTGGGGTTCT GCCCCAGAGA CCGCAGCACC
  421 GTCCCCAGCT CCTTGGTTGT TATTGTCCCA TCCCCGTCCT TGTCAAATAG GGAGAAAGCC
  481 TCTTTAAATT CTGCGATCTG CTCTTCAGTC AGTTGGTCAC GCGTGTTGTA CTCCAGCTTG
  541 TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCACGAT GCGGTTCACC
  601 AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTAGCCGTC GTCCTTGAAG
  661 AAGATGGTGC GCTCCTGGAT GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC
  721 TTCATGTGGT CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG
  781 GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG
  841 TATGTGGCAT CGCCCTCACC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG
  901 TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG
  961 CTCCCTCCGG TACCGCCCTT GTACAGCTCG TCCATGCCGA GAGTGATCCC GGCGGGCGGT
 1021 CACGAACTCC AGCAGACCAT GTGATCGCGC TTCTCGTGGG GTCTTTCGAA AGTTGGACTG
 1081 CACCGCTCAA GGTAGTTGAT GGTCGGGCAG CAGCACGGAT CGTCGTGAAT GGGGTGTTCT
1141 GCTTGTAGTT GTTAGCGGAG CTGCCACGGT CGC
//
Primer: SV40pro_F_V2 RDB14609_A7Bgc.seq
>D04021A4_A7Bg_1_SV40pro_F_V2_D12_12_ABI24.ab1
    1 AACAAACTTT TTTGGAGGCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG
   61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
  601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC
  901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG
  961 GAATCGTTTT CCCGGGACGC CGGCTGGATG ATCCCTCCAG CGCGGGGATC TCATGCTGGG
 1021 AGTTCTTCGC CCACCCTAGG GGGAGGCTAA CTGAAACACG GAAGGAGACA TACCCGGAGA
 1081 CCCGCGCTAT GACGGGCAAT AAAGACGGAA TTAAACGGCA CGGATGATGG GATCGTTTTG
1141 TTCCATATAA AACCGCCCGA G
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content Dnaconda's recommendation EXR0067j (Japanese text)
Featured content Calcium Ion Sensor Clones (English text)

References

original Ohkura, M., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011.
reference Pastrana, E., Calcium sensors reach new heights. Nat. Methods 10 (9): 824 (2013). PMID 24143827.

2017.11.22

GNP_filter3_RDBDEP_html_171106.pl