RDB14607 - G-CaMP-HS

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G-CaMP-HS (#RDB14607)

Genetically-encoded fluorescent calcium sensor cDNA, G-CaMP-HS.

Clone info.	Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. GCaMP-hyper sensitive (G-CaMP-HS) protein became more stable and stronger fluorescence than GCaMP2 in transfected mammal cells, and a GCaMP-HS is better suited to detect Ca2+ transients in vivo imaging of neuronal activities in zebrafish.
Comment	Expression was confirmed by the depositor with calcium imaging.
Vector backbone	pEGFP-N1 (plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^R (E. coli), Neo^r (mammalian cells).
Gene/insert name	Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of insert sequence	AKE44628.1 (DDBJ accession number)
Depositor\|Developer	Nakai, Junichi \| Ohkura, Masamichi \|

Sequence (full)	RDB14607hts01.seq Assembled from experimentally sequenced data.
Publication	Muto, A., Proc. Natl. Acad. Sci. U.S.A. 108 (13): 5425-5430 (2011). [link to RRC of NBRP]
Test sheet	RDB14607_A7Bgp1-2.pdf

Distribution information

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Terms and conditions for distribution In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Muto, A. et al. Proc. Nati. Acad. Sci. USA 108 (13): 5425-5430, 2011) and an acknowledgment to the DEPOSITOR are requested. The DEPOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Muto, A. et al. Proc. Nati. Acad. Sci. USA 108 (13): 5425-5430, 2011) and an acknowledgment to the DEPOSITOR are requested. The DEPOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	研究成果の公表にあたって寄託者の指定する文献(Muto, A. et al. Proc. Nati. Acad. Sci. USA 108 (13): 5425-5430, 2011)を引用し、謝辞の表明を必要とする。寄託者は、利用の状況及び成果等について利用者に報告を求めることができ、利用者は誠実に寄託者の求めに対して回答することとする。当該リソースの営利目的での利用（営利団体による利用、非営利団体が営利団体から直接・間接を問わず資金等を受けて実施する研究、非営利団体と営利団体との共同研究、非営利団体による特許取得・ライセンス契約等の行為等）の希望については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

研究成果の公表にあたって寄託者の指定する文献(Muto, A. et al. Proc. Nati. Acad. Sci. USA 108 (13): 5425-5430, 2011)を引用し、謝辞の表明を必要とする。寄託者は、利用の状況及び成果等について利用者に報告を求めることができ、利用者は誠実に寄託者の求めに対して回答することとする。当該リソースの営利目的での利用（営利団体による利用、非営利団体が営利団体から直接・間接を問わず資金等を受けて実施する研究、非営利団体と営利団体との共同研究、非営利団体による特許取得・ライセンス契約等の行為等）の希望については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14607	G-CaMP-HS	DNA solution

How to cite this biological resource

Materials & Methods section:

The G-CaMP-HS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14607).

Reference section:

Muto, A., Ohkura, M., Kotani, T., Higashijima, S., Nakai, J., Kawakami, K., Genetic visualization with an improved GCaMP calcium indicator reveals spatiotemporal activation of the spinal motor neurons in zebrafish. Proc. Natl. Acad. Sci. U.S.A. 108 (13): 5425-5430 (2011). PMID 21383146. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Muto, A., Genetic visualization with an improved GCaMP calcium indicator reveals spatiotemporal activation of the spinal motor neurons in zebrafish. Proc. Natl. Acad. Sci. U.S.A. 108 (13): 5425-5430 (2011). PMID 21383146. [link to RRC of NBRP]

Resource search

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Other resources in our bank

External Database
Rat Calm2

Chickin MLCK

Aequorea victoria GFP

Reference sequence