Resource data sheet


Genetically-encoded fluorescent calcium sensor cDNA, G-CaMP-HS.

Catalog number RDB14607
Resource name G-CaMP-HS
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. GCaMP-hyper sensitive (G-CaMP-HS) protein became more stable and stronger fluorescence than GCaMP2 in transfected mammal cells, and a GCaMP-HS is better suited to detect Ca2+ transients in vivo imaging of neuronal activities in zebrafish.

Expression was confirmed by the depositor with calcium imaging.
Vector backbone pEGFP-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^R (E. coli), Neo^r (mammalian cells).
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence AKE44628.1 (DDBJ accession number)
Depositor Nakai, Junichi | Ohkura, Masamichi |
Other clones in our bank Rat Calm2 (KEGG orthology K02183) | Chickin MYLK (KEGG orthology K00907) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Muto, A. et al. Proc. Nati. Acad. Sci. USA 108 (13): 5425-5430, 2011) and an acknowledgment to the DEPOSITOR are requested. The DEPOOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.
(Japanese text [open/close])
Remarks ((Additional form)) GFP-A
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14607 G-CaMP-HS DNA solution

Sequence information

References and tips

Electronic file

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original Muto, A., Genetic visualization with an improved GCaMP calcium indicator reveals spatiotemporal activation of the spinal motor neurons in zebrafish. Proc. Natl. Acad. Sci. U.S.A. 108 (13): 5425-5430 (2011). PMID 21383146.