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pcDNA3.1-hTRPV1-FLAG-Y511F (#RDB14598)

Expression vector human Transient receptor potential vanilloid type I (TRPV1) mutant Y511F with C-terminal FLAG-tag.

Clone info. Expression vector human Transient receptor potential vanilloid type I (TRPV1) mutant Y511F with C-terminal FLAG-tag.
Comment Espression was confirmed by the depositor with Ca2+ flux assay.
Vector backbone pcDNA3.1(+) (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TRPV1 cDNA
Depositor|Developer Ohbuchi, Katsuya |
 
Sequence (full) RDB14598hts01.seq checkAssembled from experimentally sequenced data.
Publication Ohbuchi, K., PLoS One 11 (9): e0162543 (2016). [link to RRC of NBRP]
Test sheet RDB14598_A7B9p1-1.pdf 
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ohbuchi, K. et al. PLoS One., 11 (9): e0162543, 2016)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ohbuchi, K. et al. PLoS One., 11 (9): e0162543, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14598 pcDNA3.1-hTRPV1-FLAG-Y511F DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-hTRPV1-FLAG-Y511F was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14598).

Reference section:

Ohbuchi, K., Mori, Y., Ogawa, K., Warabi, E., Yamamoto, M., Hirokawa, T., Detailed Analysis of the Binding Mode of Vanilloids to Transient Receptor Potential Vanilloid Type I (TRPV1) by a Mutational and Computational Study. PLoS One 11 (9): e0162543 (2016). PMID 27606946. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ohbuchi, K., Detailed Analysis of the Binding Mode of Vanilloids to Transient Receptor Potential Vanilloid Type I (TRPV1) by a Mutational and Computational Study. PLoS One 11 (9): e0162543 (2016). PMID 27606946. [link to RRC of NBRP]