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pcDNA3.1-hTRPV1-FLAG WT (#RDB14587)

Expression vector human Transient receptor potential vanilloid type I (TRPV1) wild type with C-terminal FLAG-tag.

Clone info. Expression vector human Transient receptor potential vanilloid type I (TRPV1) wild type with C-terminal FLAG-tag.
Comment Expression was confirmed by the depositor with Ca2+ flux assay.
Vector backbone pcDNA3.1(+) (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TRPV1 cDNA
Depositor|Developer Ohbuchi, Katsuya |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ohbuchi, K. et al. PLoS One., 11 (9): e0162543, 2016)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ohbuchi, K. et al. PLoS One., 11 (9): e0162543, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14587 pcDNA3.1-hTRPV1-FLAG WT DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-hTRPV1-FLAG WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14587).

Reference section:

Ohbuchi, K., Mori, Y., Ogawa, K., Warabi, E., Yamamoto, M., Hirokawa, T., Detailed Analysis of the Binding Mode of Vanilloids to Transient Receptor Potential Vanilloid Type I (TRPV1) by a Mutational and Computational Study. PLoS One 11 (9): e0162543 (2016). PMID 27606946. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14587_A7B9p1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,T7 pro,insert 5'(hTRPV1 WT 5')
Sequence file: RDB14587_A7B9e.seq check
>D04124A1_A7B9_1_CMV-Forward_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGNNGCN NTGGGCGGTA NGCGTGTNCG GTGGGNGGTC
   61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
  121 AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGCCACCA
  181 TGAAGAAATG GAGCAGCACA GACTTGGGGG CAGCTGCGGA CCCACTCCAA AAGGACACCT
  241 GCCCAGACCC CCTGGATGGA GACCCTAACT CCAGGCCACC TCCAGCCAAG CCCCAGCTCT
  301 CCACGGCCAA GAGCCGCACC CGGCTCTTTG GGAAGGGTGA CTCGGAGGAG GCTTTCCCGG
  361 TGGATTGCCC TCACGAGGAA GGTGAGCTGG ACTCCTGCCC GACCATCACA GTCAGCCCTG
  421 TTATCACCAT CCAGAGGCCA GGAGACGGCC CCACCGGTGC CAGGCTGCTG TCCCAGGACT
  481 CTGTCGCCGC CAGCACCGAG AAGACCCTCA GGCTCTATGA TCGCAGGAGT ATCTTTGAAG
  541 CCGTTGCTCA GAATAACTGC CAGGATCTGG AGAGCCTGCT GCTCTTCCTG CAGAAGAGCA
  601 AGAAGCACCT CACAGACAAC GAGTTCAAAG ACCCTGAGAC AGGGAAGACC TGTCTGCTGA
  661 AAGCCATGCT CAACCTGCAC GACGGACAGA ACACCACCAT CCCCCTGCTC CTGGAGATCG
  721 CGCGGCAAAC GGACAGCCTG AANGAGCTTG TCAACGCCAG CTACACGGAC AGCTACTACA
  781 AGGGCCAGAC AGCACTGCAC ATCGCCATCG ANAGACGCAA CATGGCCCTG GNGACCCTCC
  841 TGGTGGANAA CGGANCAGAC GTCCAGGCTG CGGNCNNNNG GGGANTNCTT TAANAAAACN
901 AAANGGNCGG NCNNNNNTCT ACTTCNNNGN NNNGNCCCNN NNNCCNNNNN NNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGH pA,insert 3'(FLAG,hTRPV1 WT 3')
Sequence file: RDB14587_A7B9f.seq check
>D04124A1_A7B9_1_BGH_rev2_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGGGGNNGG GCANCNACAG NTGGCTGNNN NNNAGAAGGC
   61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTCTAG ACTCGAGCGG CCGCTTACTT
  121 GTCGTCATCG TCTTTGTAGT CCTTCTCCCC GGAAGCGGCA GGACTCTTGA AGACCTCAGC
  181 GTCCTCTGGC TTCAGAGACC CTGAAAACTG TCGCAGATAA ACTTCCTCGG GCTGAGCAGA
  241 CTGCCTATCT CGAGCACTTG CCTCTCTTAA AAGGGGGACC AGGGCAAAGT TCTTCCAGTG
  301 TCTGCCTGAA ACTCTGCTTG ACCGCAGGGA GAAGCTCAGG GTGCGCTTGA CGCCCTCACA
  361 GTTGCCCGGG TCTTCGTTGA TGATGCCCAC GTTGGTGTTC CAGGTGGTCC AGTTCACCTC
  421 GTCCACCCTG AAGCACCACC GGTAGTCGTC CTTGCCATCA GGTGTGTACC CCACCTGCAG
  481 CAGCTTGCCT GAGCGGAAGG CCTTCCTCAT GCACTTAAGG AAGCTCTTCT CCGTGTCCAG
  541 GATGGTGATG GCTCTCTGCA GCTTCCAGAT GTTCTTGCTC TCCTGTGCGA TCTTGTTGAC
  601 AGTCTCACCC ATGAGGGCGA TGAGCATGTT GAGCAGGAGG ATGTAGGTGA GAATTACATA
  661 GGCCAGCAGC AGGATGATGA AGACAGCCTT GAAGTCATAG TTCTCAGTGA ACTCCAGGTC
  721 GCCCATGCCG ATGGTGAACT TGAACAGCTC CAGGCAGGTG GAGTACAGGC TGTTGTANGA
  781 NCTATCGGGG GGGCCTGCAG GCAGGCCCCC GCCACCTGNG CGANGTGGAC TCAGACGGCA
  841 NGGANTCATT CNNNCNGNCT TCAATCANCG TCACCANNGC NNNGGAAAAC CCGANNNNNA
  901 AGANNANGNA GNNAAACATG AANCNGCNNA NGNNCTCNCN NNATNATCNT NNNNNATCNT
961 GNNGNNNNNN NNNN
//
Primer: RDB14587#1 (Pr0440)
Region: insert mid(hTRPV1 WT mid)
Sequence file: RDB14587_A7B9g.seq check
>D04124A1_A7B9_1_RDB14587#1_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCNNGNNGNN GTAGGTGANA ATTACNTAGG CCAGCAGCAG
   61 GATGATGAAG ACAGCCTTGA AGTCATAGTT CTCAGTGAAC TCCAGGTCGC CCATGCCGAT
  121 GGTGAACTTG AACAGCTCCA GGCAGGTGGA GTACAGGCTG TTGTAGGAGC TATCGGGGGG
  181 CCTGCAGGCA GGCCCCCGCC ACCTGTGCGA CGTGGACTCA GACGGCAGGG AGTCATTCTT
  241 CCCGTCTTCA ATCAGCGTCA CCACCGCTGT GGAAAACCCG AACAAGAAGA CGATGTAGAC
  301 AAACATGAAA CGGCACAGGT CTCTCAGGAT CATCTTCTCT ATCATGACGG CATAGATGCC
  361 CATCTGCTGG AAACCGCGGG TGTAGTAGAG CATGTTGGTC CAGCCCAAGG CCAGGGAGAA
  421 TACCATGGAA GCCACATACT CCTTGAGGTG GCTGAAGTAC AGCACCACGG TGGCCAGCAT
  481 GAACAGTGAC TGCAGAAAGA AAAGCATCTC ACTGTAGCTG TCCACAAACA GGGTCTTCAT
  541 CGACGGCCGC CTCTGCAGGA AATACTGAAT CCCTCGGAAA AAGAAGTAGA CTCCTCCTAA
  601 CACAGACAGG ATCTCTCCAG TAACTCGGAA ATAGTCTCCA GTTTTTTCCA TCTTAAAGGG
  661 AGGCAAGCCA TCCACGGGCC TGTAGTAGGC AGCCATGGTG AAGATGATCA TGTACAGGCA
  721 GTAGACCAGG AAGTTGAAGT AGAAGATGCG CTTGACGAAT CTGTCCCACT TGTCCTGCAG
  781 GANTCGGTTC AGCGGCTCCA CCAAGANCAT GTCGNGGCGA TTANGGGTCT CGCTGCTGCT
  841 GTANGCGATC ACCTCCNGCA NCNANTTCTT CTCGCANGNG NCNANGCNNN ACAGGNCGNA
  901 CANCNANGNN NGNNCGGGNC NNNNNNCCNN NNNNGNNNNC TTNCNNNNNA GNNGNCNTGC
961 NNNNNGNNNN NNNNNN
//
Primer: SV40pro_F (Pr0110)
Region: NeoR
Sequence file: RDB14587_A7B9h.seq check
>D04124A1_A7B9_1_SV40pro_F_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGNNNNNNTT TTTTNNGNNG
   61 GCCTAGGNCT TTTNNNNNNN NNCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA
  121 AGAGACAGGA TGAGGATCGT TTCNCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC
  181 GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC
  241 TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA
  301 CCTGTCCGGT GCCCTGAATG AACTGCAGGA CNANGCAGCG CGGCTATCGT GGCTGGCCAC
  361 GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT
  421 GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA
  481 AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC
  541 ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT
  601 TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC
  661 CAGGCTCAAG GCGCGCATGC NNNNNGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG
  721 CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TNCNTCGACT GTGGNCGGCT
  781 GGGGTGTGGC GGANCGCTAT CANGACATAG CGTNNGCTAC CCGTGATATT GCTGAAGANC
  841 TTGGNNGGCG NANGGGGCTG ANCGCTTCCT CNNGCTTNAN GGTATCGCCG CTCCCGATTN
  901 NNNNNGCATC GCCNTCNATC GCCTTCTTGA CGANTNNNNC TGANCGGGNN TCNNGGGGNT
961 CNNNNGACNN ACCNNGCNNA NNNCNNNNNT GNNNNNNNNN ANNNTNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ohbuchi, K., Detailed Analysis of the Binding Mode of Vanilloids to Transient Receptor Potential Vanilloid Type I (TRPV1) by a Mutational and Computational Study. PLoS One 11 (9): e0162543 (2016). PMID 27606946. [link to RRC of NBRP]