Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA3.1-hTRPV1-FLAG WT

Expression vector human Transient receptor potential vanilloid type I (TRPV1) wild type with C-terminal FLAG-tag.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ohbuchi, K. et al. PLoS One., 11 (9): e0162543, 2016)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ohbuchi, K. et al. PLoS One., 11 (9): e0162543, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14587_A7B9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMVpro,insert5' Sequence file: RDB14587_A7B9a.seq
>D04124A1_A7B9_1_CMV-Forward_A01_01_ABI24.ab1     1 CCGGTTCACC CTGCGGCTCC AGTTGACGCA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC    61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT   121 AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGCCACCA   181 TGAAGAAATG GAGCAGCACA GACTTGGGGG CAGCTGCGGA CCCACTCCAA AAGGACACCT   241 GCCCAGACCC CCTGGATGGA GACCCTAACT CCAGGCCACC TCCAGCCAAG CCCCAGCTCT   301 CCACGGCCAA GAGCCGCACC CGGCTCTTTG GGAAGGGTGA CTCGGAGGAG GCTTTCCCGG   361 TGGATTGCCC TCACGAGGAA GGTGAGCTGG ACTCCTGCCC GACCATCACA GTCAGCCCTG   421 TTATCACCAT CCAGAGGCCA GGAGACGGCC CCACCGGTGC CAGGCTGCTG TCCCAGGACT   481 CTGTCGCCGC CAGCACCGAG AAGACCCTCA GGCTCTATGA TCGCAGGAGT ATCTTTGAAG   541 CCGTTGCTCA GAATAACTGC CAGGATCTGG AGAGCCTGCT GCTCTTCCTG CAGAAGAGCA   601 AGAAGCACCT CACAGACAAC GAGTTCAAAG ACCCTGAGAC AGGGAAGACC TGTCTGCTGA   661 AAGCCATGCT CAACCTGCAC GACGGACAGA ACACCACCAT CCCCCTGCTC CTGGAGATCG   721 CGCGGCAAAC GGACAGCCTG AAGGAGCTTG TCAACGCCAG CTACACGGAC AGCTACTACA   781 AGGGCCAGAC AGCACTGCAC ATCGCCATCG AGAGACGCAA CATGGCCCTG GTGACCCTCC   841 TGGTGGAGAA CGGAGCAGAC GTCCAGGCTG CGGCCCATGG GGGACTTCTT TAAGAAAACC   901 AAAGGGGCGG CCTGGATTCT ACTTCGGTGA ACTGCCCCCT GTCCCTGACC GCGTGCACCA   961 ACCAGCTGGG GCATCGTGAA GTTCCCTGCT GCAGAACTCC TGGTAGACGG GCCGACATCA  1021 GCGCCAGGAC TCGGTGGGGC ACACGTTGCT GCACGCCCTG AGTGGAGTTG CGGACCAACA 1081 CGGCCGGAAC GAACAACGCG AAGG //
Primer: RDB14587#1 Region: insert Sequence file: RDB14587_A7B9b.seq
>D04124A1_A7B9_1_RDB14587#1_B01_04_ABI24.ab1     1 CATTTCGGAG AGCATGTTGA GCAGGAGGAT GTAGGTGAGA ATTACATAGG CCAGCAGCAG    61 GATGATGAAG ACAGCCTTGA AGTCATAGTT CTCAGTGAAC TCCAGGTCGC CCATGCCGAT   121 GGTGAACTTG AACAGCTCCA GGCAGGTGGA GTACAGGCTG TTGTAGGAGC TATCGGGGGG   181 CCTGCAGGCA GGCCCCCGCC ACCTGTGCGA CGTGGACTCA GACGGCAGGG AGTCATTCTT   241 CCCGTCTTCA ATCAGCGTCA CCACCGCTGT GGAAAACCCG AACAAGAAGA CGATGTAGAC   301 AAACATGAAA CGGCACAGGT CTCTCAGGAT CATCTTCTCT ATCATGACGG CATAGATGCC   361 CATCTGCTGG AAACCGCGGG TGTAGTAGAG CATGTTGGTC CAGCCCAAGG CCAGGGAGAA   421 TACCATGGAA GCCACATACT CCTTGAGGTG GCTGAAGTAC AGCACCACGG TGGCCAGCAT   481 GAACAGTGAC TGCAGAAAGA AAAGCATCTC ACTGTAGCTG TCCACAAACA GGGTCTTCAT   541 CGACGGCCGC CTCTGCAGGA AATACTGAAT CCCTCGGAAA AAGAAGTAGA CTCCTCCTAA   601 CACAGACAGG ATCTCTCCAG TAACTCGGAA ATAGTCTCCA GTTTTTTCCA TCTTAAAGGG   661 AGGCAAGCCA TCCACGGGCC TGTAGTAGGC AGCCATGGTG AAGATGATCA TGTACAGGCA   721 GTAGACCAGG AAGTTGAAGT AGAAGATGCG CTTGACGAAT CTGTCCCACT TGTCCTGCAG   781 GAGTCGGTTC AGCGGCTCCA CCAAGAGCAT GTCGTGGCGA TTAGGGGTCT CGCTGCTGCT   841 GTAGGCGATC ACCTCCAGCA CCGAGTTCTT CTCGCAGGTG TCGATGCAGG ACAGGTCGTA   901 CAGCGAGGAG TGCACGGGCC CGTAGGCCCA CTCGGTGAAC TTCCTGGACA GGTGCCCTGC   961 ACTCGGGCTC CTGGATCTCC CGCTGGAGAA TATAGGCCCA GACCCCGATC TTCCCGGGTC  1021 CCAGCTGCCA GAGCCAGCGG CGTCATTTCC CTTCCTGATG GTTGAGCTCG TCAGCTCAGC 1081 GTTCGGTGCC AGTTGGACCC CCAGGAATTT CAGAAATGT //
Primer: BGH_rev2 Region: bGHp(A),FLAG,insert3' Sequence file: RDB14587_A7B9c.seq
>D04124A1_A7B9_1_BGH_rev2_C01_07_ABI24.ab1     1 CTTTCAGGGT TAAGGAAGCA CGGGGGAGGG GCAACAACAG ATGGCTGGCA ACTAGAAGGC    61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTCTAG ACTCGAGCGG CCGCTTACTT   121 GTCGTCATCG TCTTTGTAGT CCTTCTCCCC GGAAGCGGCA GGACTCTTGA AGACCTCAGC   181 GTCCTCTGGC TTCAGAGACC CTGAAAACTG TCGCAGATAA ACTTCCTCGG GCTGAGCAGA   241 CTGCCTATCT CGAGCACTTG CCTCTCTTAA AAGGGGGACC AGGGCAAAGT TCTTCCAGTG   301 TCTGCCTGAA ACTCTGCTTG ACCGCAGGGA GAAGCTCAGG GTGCGCTTGA CGCCCTCACA   361 GTTGCCCGGG TCTTCGTTGA TGATGCCCAC GTTGGTGTTC CAGGTGGTCC AGTTCACCTC   421 GTCCACCCTG AAGCACCACC GGTAGTCGTC CTTGCCATCA GGTGTGTACC CCACCTGCAG   481 CAGCTTGCCT GAGCGGAAGG CCTTCCTCAT GCACTTAAGG AAGCTCTTCT CCGTGTCCAG   541 GATGGTGATG GCTCTCTGCA GCTTCCAGAT GTTCTTGCTC TCCTGTGCGA TCTTGTTGAC   601 AGTCTCACCC ATGAGGGCGA TGAGCATGTT GAGCAGGAGG ATGTAGGTGA GAATTACATA   661 GGCCAGCAGC AGGATGATGA AGACAGCCTT GAAGTCATAG TTCTCAGTGA ACTCCAGGTC   721 GCCCATGCCG ATGGTGAACT TGAACAGCTC CAGGCAGGTG GAGTACAGGC TGTTGTAGGA   781 GCTATCGGGG GGGCCTGCAG GCAGGCCCCC GCCACCTGTG CGACGTGGAC TCAGACGGCA   841 GGGAGTCATT CTTCCCGTCT TCAATCAGCG TCACCACCGC TGTGGAAAAC CCGAACAAGA   901 AGACGATGTA GACAAACATG AAACGGCACA GGGTCTCTCA GGATCATCTT TCTCTATCAT   961 GACGGCATAG ATGCCCATCT GCTGGAAACC GGCGGGTGTA GTAGAGCATG TTTGGTCCAG  1021 GCCCAAGGTA GGGAGAATAC CATGGGAAAG GCACATACTC TTTGAAGTGC CTGAGTACAG 1081 CAACCACGGT GGCCAGGCCA ATGCAACAGG TTGAT //
Primer: SV40pro_F Region: SV40pro_ori,NeoR Sequence file: RDB14587_A7B9d.seq
>D04124A1_A7B9_1_SV40pro_F_D01_10_ABI24.ab1     1 CGTCCGCTGG TTTGGGGGCA AAACCAGGAA GTTAGGTGAA GGGAGGGCTT TTTTGGGAGG    61 GCCTAGGGCT TTTGGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA   121 AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC   181 GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC   241 TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA   301 CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC   361 GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT   421 GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA   481 AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC   541 ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT   601 TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC   661 CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG   721 CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT   781 GGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC   841 TTGGGCGGCG AATGGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC   901 GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGGTT   961 CGAATGACCG ACCAAGCGGA CGCCCAACCT GCCATCACCG AGATTTTCGG ATTCCACCGT  1021 CGGCCTTCTA TGAAGTTGGG CTTTCGGGAA TCGTTTTCCG GGACGCCGCT GGATGAATCC 1081 TTCCAGCGCG GGATCTCAAT GCCTGGAGTT CCTTTCGCCA CCCCGAACT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl