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pcDNA3.1-hTRPV1-FLAG WT

Expression vector human Transient receptor potential vanilloid type I (TRPV1) wild type with C-terminal FLAG-tag.

Catalog number RDB14587
Resource name pcDNA3.1-hTRPV1-FLAG WT
Clone info. Expression vector human Transient receptor potential vanilloid type I (TRPV1) wild type with C-terminal FLAG-tag.
Espression was confirmed by the depositor with Ca2+ flux assay.
Vector backbone pcDNA3.1(+) (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TRPV1 cDNA
Depositor Ohbuchi, Katsuya |
 
Other clones in our bank human TRPV1 (NCBI Gene 7442) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ohbuchi, K. et al. PLoS One., 11 (9): e0162543, 2016)
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RDB14587 pcDNA3.1-hTRPV1-FLAG WT DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Ohbuchi, K., Detailed Analysis of the Binding Mode of Vanilloids to Transient Receptor Potential Vanilloid Type I (TRPV1) by a Mutational and Computational Study. PLoS One 11 (9): e0162543 (2016). PMID 27606946.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14587_A7B9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: CMVpro,insert5' | Primer: CMV_Forward | Sequence: RDB14587_A7B9a.seq
>D04124A1_A7B9_1_CMV-Forward_A01_01_ABI24.ab1
    1 CCGGTTCACC CTGCGGCTCC AGTTGACGCA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
  121 AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGCCACCA
  181 TGAAGAAATG GAGCAGCACA GACTTGGGGG CAGCTGCGGA CCCACTCCAA AAGGACACCT
  241 GCCCAGACCC CCTGGATGGA GACCCTAACT CCAGGCCACC TCCAGCCAAG CCCCAGCTCT
  301 CCACGGCCAA GAGCCGCACC CGGCTCTTTG GGAAGGGTGA CTCGGAGGAG GCTTTCCCGG
  361 TGGATTGCCC TCACGAGGAA GGTGAGCTGG ACTCCTGCCC GACCATCACA GTCAGCCCTG
  421 TTATCACCAT CCAGAGGCCA GGAGACGGCC CCACCGGTGC CAGGCTGCTG TCCCAGGACT
  481 CTGTCGCCGC CAGCACCGAG AAGACCCTCA GGCTCTATGA TCGCAGGAGT ATCTTTGAAG
  541 CCGTTGCTCA GAATAACTGC CAGGATCTGG AGAGCCTGCT GCTCTTCCTG CAGAAGAGCA
  601 AGAAGCACCT CACAGACAAC GAGTTCAAAG ACCCTGAGAC AGGGAAGACC TGTCTGCTGA
  661 AAGCCATGCT CAACCTGCAC GACGGACAGA ACACCACCAT CCCCCTGCTC CTGGAGATCG
  721 CGCGGCAAAC GGACAGCCTG AAGGAGCTTG TCAACGCCAG CTACACGGAC AGCTACTACA
  781 AGGGCCAGAC AGCACTGCAC ATCGCCATCG AGAGACGCAA CATGGCCCTG GTGACCCTCC
  841 TGGTGGAGAA CGGAGCAGAC GTCCAGGCTG CGGCCCATGG GGGACTTCTT TAAGAAAACC
  901 AAAGGGGCGG CCTGGATTCT ACTTCGGTGA ACTGCCCCCT GTCCCTGACC GCGTGCACCA
  961 ACCAGCTGGG GCATCGTGAA GTTCCCTGCT GCAGAACTCC TGGTAGACGG GCCGACATCA
 1021 GCGCCAGGAC TCGGTGGGGC ACACGTTGCT GCACGCCCTG AGTGGAGTTG CGGACCAACA
1081 CGGCCGGAAC GAACAACGCG AAGG
//
Region: insert | Primer: RDB14587#1 | Sequence: RDB14587_A7B9b.seq
>D04124A1_A7B9_1_RDB14587#1_B01_04_ABI24.ab1
    1 CATTTCGGAG AGCATGTTGA GCAGGAGGAT GTAGGTGAGA ATTACATAGG CCAGCAGCAG
   61 GATGATGAAG ACAGCCTTGA AGTCATAGTT CTCAGTGAAC TCCAGGTCGC CCATGCCGAT
  121 GGTGAACTTG AACAGCTCCA GGCAGGTGGA GTACAGGCTG TTGTAGGAGC TATCGGGGGG
  181 CCTGCAGGCA GGCCCCCGCC ACCTGTGCGA CGTGGACTCA GACGGCAGGG AGTCATTCTT
  241 CCCGTCTTCA ATCAGCGTCA CCACCGCTGT GGAAAACCCG AACAAGAAGA CGATGTAGAC
  301 AAACATGAAA CGGCACAGGT CTCTCAGGAT CATCTTCTCT ATCATGACGG CATAGATGCC
  361 CATCTGCTGG AAACCGCGGG TGTAGTAGAG CATGTTGGTC CAGCCCAAGG CCAGGGAGAA
  421 TACCATGGAA GCCACATACT CCTTGAGGTG GCTGAAGTAC AGCACCACGG TGGCCAGCAT
  481 GAACAGTGAC TGCAGAAAGA AAAGCATCTC ACTGTAGCTG TCCACAAACA GGGTCTTCAT
  541 CGACGGCCGC CTCTGCAGGA AATACTGAAT CCCTCGGAAA AAGAAGTAGA CTCCTCCTAA
  601 CACAGACAGG ATCTCTCCAG TAACTCGGAA ATAGTCTCCA GTTTTTTCCA TCTTAAAGGG
  661 AGGCAAGCCA TCCACGGGCC TGTAGTAGGC AGCCATGGTG AAGATGATCA TGTACAGGCA
  721 GTAGACCAGG AAGTTGAAGT AGAAGATGCG CTTGACGAAT CTGTCCCACT TGTCCTGCAG
  781 GAGTCGGTTC AGCGGCTCCA CCAAGAGCAT GTCGTGGCGA TTAGGGGTCT CGCTGCTGCT
  841 GTAGGCGATC ACCTCCAGCA CCGAGTTCTT CTCGCAGGTG TCGATGCAGG ACAGGTCGTA
  901 CAGCGAGGAG TGCACGGGCC CGTAGGCCCA CTCGGTGAAC TTCCTGGACA GGTGCCCTGC
  961 ACTCGGGCTC CTGGATCTCC CGCTGGAGAA TATAGGCCCA GACCCCGATC TTCCCGGGTC
 1021 CCAGCTGCCA GAGCCAGCGG CGTCATTTCC CTTCCTGATG GTTGAGCTCG TCAGCTCAGC
1081 GTTCGGTGCC AGTTGGACCC CCAGGAATTT CAGAAATGT
//
Region: bGHp(A),FLAG,insert3' | Primer: BGH_rev2 | Sequence: RDB14587_A7B9c.seq
>D04124A1_A7B9_1_BGH_rev2_C01_07_ABI24.ab1
    1 CTTTCAGGGT TAAGGAAGCA CGGGGGAGGG GCAACAACAG ATGGCTGGCA ACTAGAAGGC
   61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTCTAG ACTCGAGCGG CCGCTTACTT
  121 GTCGTCATCG TCTTTGTAGT CCTTCTCCCC GGAAGCGGCA GGACTCTTGA AGACCTCAGC
  181 GTCCTCTGGC TTCAGAGACC CTGAAAACTG TCGCAGATAA ACTTCCTCGG GCTGAGCAGA
  241 CTGCCTATCT CGAGCACTTG CCTCTCTTAA AAGGGGGACC AGGGCAAAGT TCTTCCAGTG
  301 TCTGCCTGAA ACTCTGCTTG ACCGCAGGGA GAAGCTCAGG GTGCGCTTGA CGCCCTCACA
  361 GTTGCCCGGG TCTTCGTTGA TGATGCCCAC GTTGGTGTTC CAGGTGGTCC AGTTCACCTC
  421 GTCCACCCTG AAGCACCACC GGTAGTCGTC CTTGCCATCA GGTGTGTACC CCACCTGCAG
  481 CAGCTTGCCT GAGCGGAAGG CCTTCCTCAT GCACTTAAGG AAGCTCTTCT CCGTGTCCAG
  541 GATGGTGATG GCTCTCTGCA GCTTCCAGAT GTTCTTGCTC TCCTGTGCGA TCTTGTTGAC
  601 AGTCTCACCC ATGAGGGCGA TGAGCATGTT GAGCAGGAGG ATGTAGGTGA GAATTACATA
  661 GGCCAGCAGC AGGATGATGA AGACAGCCTT GAAGTCATAG TTCTCAGTGA ACTCCAGGTC
  721 GCCCATGCCG ATGGTGAACT TGAACAGCTC CAGGCAGGTG GAGTACAGGC TGTTGTAGGA
  781 GCTATCGGGG GGGCCTGCAG GCAGGCCCCC GCCACCTGTG CGACGTGGAC TCAGACGGCA
  841 GGGAGTCATT CTTCCCGTCT TCAATCAGCG TCACCACCGC TGTGGAAAAC CCGAACAAGA
  901 AGACGATGTA GACAAACATG AAACGGCACA GGGTCTCTCA GGATCATCTT TCTCTATCAT
  961 GACGGCATAG ATGCCCATCT GCTGGAAACC GGCGGGTGTA GTAGAGCATG TTTGGTCCAG
 1021 GCCCAAGGTA GGGAGAATAC CATGGGAAAG GCACATACTC TTTGAAGTGC CTGAGTACAG
1081 CAACCACGGT GGCCAGGCCA ATGCAACAGG TTGAT
//
Region: SV40pro_ori,NeoR | Primer: SV40pro_F | Sequence: RDB14587_A7B9d.seq
>D04124A1_A7B9_1_SV40pro_F_D01_10_ABI24.ab1
    1 CGTCCGCTGG TTTGGGGGCA AAACCAGGAA GTTAGGTGAA GGGAGGGCTT TTTTGGGAGG
   61 GCCTAGGGCT TTTGGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA
  121 AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC
  181 GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC
  241 TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA
  301 CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC
  361 GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT
  421 GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA
  481 AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC
  541 ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT
  601 TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC
  661 CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG
  721 CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT
  781 GGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC
  841 TTGGGCGGCG AATGGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC
  901 GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGGTT
  961 CGAATGACCG ACCAAGCGGA CGCCCAACCT GCCATCACCG AGATTTTCGG ATTCCACCGT
 1021 CGGCCTTCTA TGAAGTTGGG CTTTCGGGAA TCGTTTTCCG GGACGCCGCT GGATGAATCC
1081 TTCCAGCGCG GGATCTCAAT GCCTGGAGTT CCTTTCGCCA CCCCGAACT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ohbuchi, K., Detailed Analysis of the Binding Mode of Vanilloids to Transient Receptor Potential Vanilloid Type I (TRPV1) by a Mutational and Computational Study. PLoS One 11 (9): e0162543 (2016). PMID 27606946.

2019.10.20

GNP_filter3_RDBDEP_html_190927.pl