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pCMV-Myc-Batf (#RDB14572)

Expression vector of Myc-tagged mouse Batf.

Clone info. Expression vector of Myc-tagged mouse Batf.
Comment Expression was confirmed by the depositor with immunoprecipitation and immunoblot using anti Myc antibody, respectively.
Vector backbone pCMV-Myc (plasmid)
Size of vector backbone 3.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Batf cDNA
Depositor|Developer Yamashita, Masakatsu |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14572 pCMV-Myc-Batf DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMV-Myc-Batf was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14572).

Reference section:

Kuwahara, M., Ise, W., Ochi, M., Suzuki, J., Kometani, K., Maruyama, S., Izumoto, M., Matsumoto, A., Takemori, N., Takemori, A., Shinoda, K., Nakayama, T., Ohara, O., Yasukawa, M., Sawasaki, T., Kurosaki, T., Yamashita, M., Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. Nat. Commun. 7: 12596 (2016). PMID 27581382. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14572_A7B6p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14572_A7B6a.seq check
>D04207A4_A7B6_1_CMV Forward_G12_07_ABI08.ab1
    1 ATGTTTAACC TCCGCCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT
  121 GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGGA CTCTAGAGGA TCCGGTACTA
  181 GAGGAACTGA AAAACCAGAA AGTTAACTGG TAAGTTTAGT CTTTTTGTCT TTTATTTCAG
  241 GTCCCGGATC CGGTGGTGGT GCAAATCAAA GAACTGCTCC TCAGTGGATG TTGCCTTTAC
  301 TTCTAGGCCT GTACGGAAGT GTTACTTCTG CTCTAAAAGC TGCGGAATTG TACCCGCGGG
  361 CCCACCATGG CATCAATGCA GAAGCTGATC TCAGAGGAGG ACCTGCTTAT GGCCATGGAG
  421 GCCCGAATTC CCATGCCTCA CAGCTCCGAC AGCAGTGACT CCAGCTTCAG CCGCTCTCCT
  481 CCCCCTGGCA AACAGGACTC ATCTGATGAT GTGAGGAAAG TTCAGAGGAG AGAGAAGAAT
  541 CGCATCGCTG CCCAGAAGAG CCGACAGAGA CAGACACAGA AAGCCGACAC CCTTCACCTG
  601 GAGAGTGAGG ACCTGGAGAA ACAGAACGCA GCTCTCCGCA AAGAGATCAA ACAGCTCACC
  661 GAGGAGCTCA AGTACTTCAC ATCAGTGCTG AGCAGCCACG AGCCCCTGTG CTCCGTGCTG
  721 GCCAGTGGCA CCCCCTCGCC CCCCGAGGTG GTATACAGTG CCCATGCCTT CCACCAGCCT
  781 CACATCAGCT CGCCACGCTT CCAGCCCTGA CTCGAGGTAC CGCGGCCGCG GGGATCCAGA
  841 CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG
  901 CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA
  961 ACAAGTTAAC AACAACAATT TGCATTCATT TTATGTTCAG TTCAGGGGAG GTGTGGGAGG
 1021 GTTTTTCGGA TCTCTTAGAG TCGATCTGCA GGCCATGCTA GCCTTGGCGT ATCCATGGTC
1081 ATAGGCCTTG ATTTCCTGTT GTGGAAAGTT AGGTAAATC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kuwahara, M., Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. Nat. Commun. 7: 12596 (2016). PMID 27581382. [link to RRC of NBRP]