Resource data sheet
Prev.

pCMV-HA-Bach2 L694A

Expression vector of HA-tagged mouse Bach2, L694A mutant.

Catalog number RDB14571
Resource name pCMV-HA-Bach2 L694A
Clone info. Expression vector of HA-tagged mouse Bach2 point mutation into the leucine zipper region, L694A, which abrogates interaction with Batf. Refer Fig. 5 of Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016.

Expression was confirmed by the depositor with immunoprecipitation and immunoblot using anti HA antibody, respectively.
Vector backbone pCMV-HA (plasmid)
Size of vector backbone 3.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Bach2 cDNA
Depositor Yamashita, Masakatsu |
 
Other clones in our bank mouse Bach2 (NCBI Gene 12014) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016)
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14571 pCMV-HA-Bach2 L694A DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Kuwahara, M., Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. Nat. Commun. 7: 12596 (2016). PMID 27581382.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14571_A7B6p1_2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB14571_A7B6a.seq
>D04207A3_A7B6_1_CMV Forward_E12_05_ABI08.ab1
    1 AGGTTCCACT CGGCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAT CGCCTGGAGA CGCCATCCAC GCTGTTTTGA
  121 CCTCCATAGA AGACACCGGG ACCGATCCAG CCTCCGGACT CTAGAGGATC CGGTACTAGA
  181 GGAACTGAAA AACCAGAAAG TTAACTGGTA AGTTTAGTCT TTTTGTCTTT TATTTCAGGT
  241 CCCGGATCCG GTGGTGGTGC AAATCAAAGA ACTGCTCCTC AGTGGATGTT GCCTTTACTT
  301 CTAGGCCTGT ACGGAAGTGT TACTTCTGCT CTAAAAGCTG CGGAATTGTA CCCGCGGGCC
  361 CACCATGTAC CCATACGATG TTCCAGATTA CGCTCTTATG GCCATGGAGG CCCGAATTCG
  421 GTCGACCATG TCTGTGGATG AGAAGCCTGG CTCCCCCATG TATGTATATG AGTCCACAGT
  481 CCACTGTGCC AACATCCTCC TGGGCCTCAA TGACCAACGG AAGAAGGACA TTCTCTGTGA
  541 CGTGACGCTG ATCGTGGAGA GGAAGGAGTT CCGAGCCCAC CGGGCTGTGC TGGCCGCATG
  601 CAGTGAATAC TTCTGGCAAG CACTGGTTGG ACAGACGAAA GATGACTTGG TGGTCAGCTT
  661 GCCTGAGGAG GTCACGGCCA GGGGCTTTGG GCCACTGCTA CAGTTTGCCT ACACTGCCAA
  721 GCTGTTACTC AGCAGAGAGA ACATCCGGGA GGTCATCCGC TGTGCTGAGT TCCTGCGCAT
  781 GCACAATCTG GAGGACTCCT GCTTCAGCTT CCTGCAGACC CAGCTCCTGA ACAGGGAGGA
  841 TGGCCTGTTT GTTTGCAGAA AGGACAGTGC GTGCCAGCGC CCCCAAGAGG ACCATGGGAA
  901 TTCTGCAGGA GAAGAGGAGG AAGAAGAGGA AACAATGGAC TCAGAGACAG CTAGGGATGG
  961 CTTGCGCCAC AGACCAGATG CTCCCAGACC CCATCAGCTT TGAGGCCACT GCCATCCCAG
 1021 TAGCAGAGAA GAGGAGCCTG CTCCCTGAGT CTGAAGTAAC CCACAGAACA CTAGGAGACT
1081 TCAGAAAGGA TGCCAATTGG ACCCAGTACC TTAGTAATAT TAAGGAAGT
//
Primer: pAxCALNLF1 RDB14571_A7B6b.seq
>D04207A3_A7B6_1_pAxCALNLF1_F12_06_ABI08.ab1
    1 CCAAGGTATC TTTAATCATG TCTGGATCCC CGCGGCCGCC TAGGCATAAT CTTTCCTGGG
   61 CTGTTCGTCC GTTGTACACT TCTCAGTCAT TTCCTGGCAG AAGTCCACCG TCACAGACTG
  121 GCTCCTGGCT TCTAGGGGAG GTCCTCGTTC TGAGAATGTT CCAGGGTCAC TGCCTTCCAA
  181 CCTCCTCCCA GAGGTACAAT TCTCAGAGGT GTTGCTGGGC ACCCAGGGGA GCCCGGGGGG
  241 AGCCGCCCCT GGCTCCAGGC AGCAGGGTAC ACTTCCTCCT ACTGCACACG GGGAGGGTGC
  301 AAGGCTCTGC TCAACACCCA CAGGAGGAGG GTTAACACTG GCTCCAGGGA GATCCATGGG
  361 TATGAGGACA GGGCAGTAGC GGTGCAGGGC CTGGATCTGC TCTGGACTCT GGATGTCTCG
  421 ACAGACCTCC TGGGAGAGGC AGGAAAAGTT GTCCAGGAGC TCTCCCATGC ACGCCTTTGC
  481 GTGATTCCTC TCCGACAACA GTTTTTCCTT CTCGCACACC AGTTTCCGTA TTTCACATTC
  541 CAAGTTCTGA ATACAGTCCA GCTTCCTCTT TCGACAGCGC TGGGCTGCAA TGCGGTTCTT
  601 ACTCCGCCTC CGGATGTCGT GGATGAACTC CAACTGTTCT GAGGTTAGCT TGTGCATTTT
  661 GATCATCATC TGGAAGTCGT TCCTGGGAAG GTCTGTGATC TGATCGACAG GAAAAGGAAG
  721 TTTAACCTCC TGGCCCCTGT CCTGCACAGG ACACGACTCA CTGTCTGCTT CCGAGAATGA
  781 TCCAGACTCG TCACTGGAAA TTGGGTTCCG TAAGACTGCT CACATTTAAT CTGGGGTCGG
  841 GACTTGGGTT GTACATTCCA TCTCCCATCA GCCTGGGTTC CTGATGTTCC GTGCAAGGAA
  901 ATCTGGCTCC CTGGAACAGG GCGGAGAGAG AACCTCCACA GAAGAAGGAG CTGCAGGGGA
  961 GCCTCACTCC CCGTTCTCTG CGTAAGGATA CGAAGGAGCA AGGAACTGAG TTCTAGTCCT
 1021 GTCTCAAACC GAGTGAACGA AGAACGACCT GATTGGCACC GGGCAACTGG TTAGGCGAAT
1081 CTTCCTGCCA GGTGCTCAGC CCCT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kuwahara, M., Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. Nat. Commun. 7: 12596 (2016). PMID 27581382.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl