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pCMV-HA-Bach2 L694A

Expression vector of HA-tagged mouse Bach2, L694A mutant.

Catalog number RDB14571
Resource name pCMV-HA-Bach2 L694A
Clone info. Expression vector of HA-tagged mouse Bach2 point mutation into the leucine zipper region, L694A, which abrogates interaction with Batf. Refer Fig. 5 of Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016.
Comment Expression was confirmed by the depositor with immunoprecipitation and immunoblot using anti HA antibody, respectively.
Vector backbone pCMV-HA (plasmid)
Size of vector backbone 3.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Bach2 cDNA
Depositor|Developer Yamashita, Masakatsu |
Other clones in our bank
External Database
Mouse Bach2
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016)。
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          RDB14571 pCMV-HA-Bach2 L694A DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kuwahara, M., Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. Nat. Commun. 7: 12596 (2016). PMID 27581382.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14571_A7B6p1_2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB14571_A7B6a.seq check
          >D04207A3_A7B6_1_CMV Forward_E12_05_ABI08.ab1
              1 AGGTTCCACT CGGCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAT CGCCTGGAGA CGCCATCCAC GCTGTTTTGA
            121 CCTCCATAGA AGACACCGGG ACCGATCCAG CCTCCGGACT CTAGAGGATC CGGTACTAGA
            181 GGAACTGAAA AACCAGAAAG TTAACTGGTA AGTTTAGTCT TTTTGTCTTT TATTTCAGGT
            241 CCCGGATCCG GTGGTGGTGC AAATCAAAGA ACTGCTCCTC AGTGGATGTT GCCTTTACTT
            301 CTAGGCCTGT ACGGAAGTGT TACTTCTGCT CTAAAAGCTG CGGAATTGTA CCCGCGGGCC
            361 CACCATGTAC CCATACGATG TTCCAGATTA CGCTCTTATG GCCATGGAGG CCCGAATTCG
            421 GTCGACCATG TCTGTGGATG AGAAGCCTGG CTCCCCCATG TATGTATATG AGTCCACAGT
            481 CCACTGTGCC AACATCCTCC TGGGCCTCAA TGACCAACGG AAGAAGGACA TTCTCTGTGA
            541 CGTGACGCTG ATCGTGGAGA GGAAGGAGTT CCGAGCCCAC CGGGCTGTGC TGGCCGCATG
            601 CAGTGAATAC TTCTGGCAAG CACTGGTTGG ACAGACGAAA GATGACTTGG TGGTCAGCTT
            661 GCCTGAGGAG GTCACGGCCA GGGGCTTTGG GCCACTGCTA CAGTTTGCCT ACACTGCCAA
            721 GCTGTTACTC AGCAGAGAGA ACATCCGGGA GGTCATCCGC TGTGCTGAGT TCCTGCGCAT
            781 GCACAATCTG GAGGACTCCT GCTTCAGCTT CCTGCAGACC CAGCTCCTGA ACAGGGAGGA
            841 TGGCCTGTTT GTTTGCAGAA AGGACAGTGC GTGCCAGCGC CCCCAAGAGG ACCATGGGAA
            901 TTCTGCAGGA GAAGAGGAGG AAGAAGAGGA AACAATGGAC TCAGAGACAG CTAGGGATGG
            961 CTTGCGCCAC AGACCAGATG CTCCCAGACC CCATCAGCTT TGAGGCCACT GCCATCCCAG
           1021 TAGCAGAGAA GAGGAGCCTG CTCCCTGAGT CTGAAGTAAC CCACAGAACA CTAGGAGACT
          1081 TCAGAAAGGA TGCCAATTGG ACCCAGTACC TTAGTAATAT TAAGGAAGT
          //
          Primer: pAxCALNLF1
          Sequence file: RDB14571_A7B6b.seq check
          >D04207A3_A7B6_1_pAxCALNLF1_F12_06_ABI08.ab1
              1 CCAAGGTATC TTTAATCATG TCTGGATCCC CGCGGCCGCC TAGGCATAAT CTTTCCTGGG
             61 CTGTTCGTCC GTTGTACACT TCTCAGTCAT TTCCTGGCAG AAGTCCACCG TCACAGACTG
            121 GCTCCTGGCT TCTAGGGGAG GTCCTCGTTC TGAGAATGTT CCAGGGTCAC TGCCTTCCAA
            181 CCTCCTCCCA GAGGTACAAT TCTCAGAGGT GTTGCTGGGC ACCCAGGGGA GCCCGGGGGG
            241 AGCCGCCCCT GGCTCCAGGC AGCAGGGTAC ACTTCCTCCT ACTGCACACG GGGAGGGTGC
            301 AAGGCTCTGC TCAACACCCA CAGGAGGAGG GTTAACACTG GCTCCAGGGA GATCCATGGG
            361 TATGAGGACA GGGCAGTAGC GGTGCAGGGC CTGGATCTGC TCTGGACTCT GGATGTCTCG
            421 ACAGACCTCC TGGGAGAGGC AGGAAAAGTT GTCCAGGAGC TCTCCCATGC ACGCCTTTGC
            481 GTGATTCCTC TCCGACAACA GTTTTTCCTT CTCGCACACC AGTTTCCGTA TTTCACATTC
            541 CAAGTTCTGA ATACAGTCCA GCTTCCTCTT TCGACAGCGC TGGGCTGCAA TGCGGTTCTT
            601 ACTCCGCCTC CGGATGTCGT GGATGAACTC CAACTGTTCT GAGGTTAGCT TGTGCATTTT
            661 GATCATCATC TGGAAGTCGT TCCTGGGAAG GTCTGTGATC TGATCGACAG GAAAAGGAAG
            721 TTTAACCTCC TGGCCCCTGT CCTGCACAGG ACACGACTCA CTGTCTGCTT CCGAGAATGA
            781 TCCAGACTCG TCACTGGAAA TTGGGTTCCG TAAGACTGCT CACATTTAAT CTGGGGTCGG
            841 GACTTGGGTT GTACATTCCA TCTCCCATCA GCCTGGGTTC CTGATGTTCC GTGCAAGGAA
            901 ATCTGGCTCC CTGGAACAGG GCGGAGAGAG AACCTCCACA GAAGAAGGAG CTGCAGGGGA
            961 GCCTCACTCC CCGTTCTCTG CGTAAGGATA CGAAGGAGCA AGGAACTGAG TTCTAGTCCT
           1021 GTCTCAAACC GAGTGAACGA AGAACGACCT GATTGGCACC GGGCAACTGG TTAGGCGAAT
          1081 CTTCCTGCCA GGTGCTCAGC CCCT
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Kuwahara, M., Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. Nat. Commun. 7: 12596 (2016). PMID 27581382.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl