Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMV-HA-Bach2 L694A (#RDB14571)

Expression vector of HA-tagged mouse Bach2, L694A mutant.

Clone info. Expression vector of HA-tagged mouse Bach2 point mutation into the leucine zipper region, L694A, which abrogates interaction with Batf. Refer Fig. 5 of Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016.
Comment Expression was confirmed by the depositor with immunoprecipitation and immunoblot using anti HA antibody, respectively.
Vector backbone pCMV-HA (plasmid)
Size of vector backbone 3.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Bach2 cDNA
Depositor|Developer Yamashita, Masakatsu |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14571 pCMV-HA-Bach2 L694A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMV-HA-Bach2 L694A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14571).

Reference section:

Kuwahara, M., Ise, W., Ochi, M., Suzuki, J., Kometani, K., Maruyama, S., Izumoto, M., Matsumoto, A., Takemori, N., Takemori, A., Shinoda, K., Nakayama, T., Ohara, O., Yasukawa, M., Sawasaki, T., Kurosaki, T., Yamashita, M., Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. Nat. Commun. 7: 12596 (2016). PMID 27581382. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14571_A7B6p1_2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14571_A7B6a.seq check
>D04207A3_A7B6_1_CMV Forward_E12_05_ABI08.ab1
    1 AGGTTCCACT CGGCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAT CGCCTGGAGA CGCCATCCAC GCTGTTTTGA
  121 CCTCCATAGA AGACACCGGG ACCGATCCAG CCTCCGGACT CTAGAGGATC CGGTACTAGA
  181 GGAACTGAAA AACCAGAAAG TTAACTGGTA AGTTTAGTCT TTTTGTCTTT TATTTCAGGT
  241 CCCGGATCCG GTGGTGGTGC AAATCAAAGA ACTGCTCCTC AGTGGATGTT GCCTTTACTT
  301 CTAGGCCTGT ACGGAAGTGT TACTTCTGCT CTAAAAGCTG CGGAATTGTA CCCGCGGGCC
  361 CACCATGTAC CCATACGATG TTCCAGATTA CGCTCTTATG GCCATGGAGG CCCGAATTCG
  421 GTCGACCATG TCTGTGGATG AGAAGCCTGG CTCCCCCATG TATGTATATG AGTCCACAGT
  481 CCACTGTGCC AACATCCTCC TGGGCCTCAA TGACCAACGG AAGAAGGACA TTCTCTGTGA
  541 CGTGACGCTG ATCGTGGAGA GGAAGGAGTT CCGAGCCCAC CGGGCTGTGC TGGCCGCATG
  601 CAGTGAATAC TTCTGGCAAG CACTGGTTGG ACAGACGAAA GATGACTTGG TGGTCAGCTT
  661 GCCTGAGGAG GTCACGGCCA GGGGCTTTGG GCCACTGCTA CAGTTTGCCT ACACTGCCAA
  721 GCTGTTACTC AGCAGAGAGA ACATCCGGGA GGTCATCCGC TGTGCTGAGT TCCTGCGCAT
  781 GCACAATCTG GAGGACTCCT GCTTCAGCTT CCTGCAGACC CAGCTCCTGA ACAGGGAGGA
  841 TGGCCTGTTT GTTTGCAGAA AGGACAGTGC GTGCCAGCGC CCCCAAGAGG ACCATGGGAA
  901 TTCTGCAGGA GAAGAGGAGG AAGAAGAGGA AACAATGGAC TCAGAGACAG CTAGGGATGG
  961 CTTGCGCCAC AGACCAGATG CTCCCAGACC CCATCAGCTT TGAGGCCACT GCCATCCCAG
 1021 TAGCAGAGAA GAGGAGCCTG CTCCCTGAGT CTGAAGTAAC CCACAGAACA CTAGGAGACT
1081 TCAGAAAGGA TGCCAATTGG ACCCAGTACC TTAGTAATAT TAAGGAAGT
//
Primer: pAxCALNLF1
Sequence file: RDB14571_A7B6b.seq check
>D04207A3_A7B6_1_pAxCALNLF1_F12_06_ABI08.ab1
    1 CCAAGGTATC TTTAATCATG TCTGGATCCC CGCGGCCGCC TAGGCATAAT CTTTCCTGGG
   61 CTGTTCGTCC GTTGTACACT TCTCAGTCAT TTCCTGGCAG AAGTCCACCG TCACAGACTG
  121 GCTCCTGGCT TCTAGGGGAG GTCCTCGTTC TGAGAATGTT CCAGGGTCAC TGCCTTCCAA
  181 CCTCCTCCCA GAGGTACAAT TCTCAGAGGT GTTGCTGGGC ACCCAGGGGA GCCCGGGGGG
  241 AGCCGCCCCT GGCTCCAGGC AGCAGGGTAC ACTTCCTCCT ACTGCACACG GGGAGGGTGC
  301 AAGGCTCTGC TCAACACCCA CAGGAGGAGG GTTAACACTG GCTCCAGGGA GATCCATGGG
  361 TATGAGGACA GGGCAGTAGC GGTGCAGGGC CTGGATCTGC TCTGGACTCT GGATGTCTCG
  421 ACAGACCTCC TGGGAGAGGC AGGAAAAGTT GTCCAGGAGC TCTCCCATGC ACGCCTTTGC
  481 GTGATTCCTC TCCGACAACA GTTTTTCCTT CTCGCACACC AGTTTCCGTA TTTCACATTC
  541 CAAGTTCTGA ATACAGTCCA GCTTCCTCTT TCGACAGCGC TGGGCTGCAA TGCGGTTCTT
  601 ACTCCGCCTC CGGATGTCGT GGATGAACTC CAACTGTTCT GAGGTTAGCT TGTGCATTTT
  661 GATCATCATC TGGAAGTCGT TCCTGGGAAG GTCTGTGATC TGATCGACAG GAAAAGGAAG
  721 TTTAACCTCC TGGCCCCTGT CCTGCACAGG ACACGACTCA CTGTCTGCTT CCGAGAATGA
  781 TCCAGACTCG TCACTGGAAA TTGGGTTCCG TAAGACTGCT CACATTTAAT CTGGGGTCGG
  841 GACTTGGGTT GTACATTCCA TCTCCCATCA GCCTGGGTTC CTGATGTTCC GTGCAAGGAA
  901 ATCTGGCTCC CTGGAACAGG GCGGAGAGAG AACCTCCACA GAAGAAGGAG CTGCAGGGGA
  961 GCCTCACTCC CCGTTCTCTG CGTAAGGATA CGAAGGAGCA AGGAACTGAG TTCTAGTCCT
 1021 GTCTCAAACC GAGTGAACGA AGAACGACCT GATTGGCACC GGGCAACTGG TTAGGCGAAT
1081 CTTCCTGCCA GGTGCTCAGC CCCT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kuwahara, M., Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. Nat. Commun. 7: 12596 (2016). PMID 27581382. [link to RRC of NBRP]