Resource data sheet


Expression vector of Ramazzottius varieornatus Dsup.

Catalog number RDB14553
Resource name pCold-I-Dsup
Clone info. Bacteria expression vector of N-terminal 6xHis tagged Ramazzottius varieornatus Dsup for expression recombinant protein in E. coli.
Expression was confirmed by the depositor.
Vector backbone pCold I (Plasmid)
Size of vector backbone 4.4 kb
Selectable markers Amp^r
Gene/insert name Ramazzottius varieornatus Dsup cDNA
Reference of nucleotide sequence LC050827 (DDBJ accession number)
Depositor Kunieda, Takekazu | Hashimoto, Takuma |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Hashimoto, T. et al. Nature Commu. 7, 12808, 2016).
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14553 pCold-I-Dsup DNA solution

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Electronic file

Featured content


original Hashimoto, T., Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Nat. Commun. 7: 12808 (2016). PMID 27649274.