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pAcGFP1-N1-Dsup (#RDB14552)

Expression vector of GFP-fused Ramazzottius varieornatus Dsup.

Clone info. Expression vector of GFP (AcGFP)-fused Ramazzottius varieornatus Dsup under the CMV promoter for transient expression in mammalian cells.
Comment Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pAcGFP1-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Ramazzottius varieornatus Dsup cDNA
Reference of insert sequence LC050827 (DDBJ accession number)
Depositor|Developer Kunieda, Takekazu | Hashimoto, Takuma |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Hashimoto, T. et al. Nature Commu. 7, 12808, 2016).
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hashimoto, T. et al. Nature Commu. 7, 12808, 2016)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14552 pAcGFP1-N1-Dsup Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

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How to cite this biological resource

Materials & Methods section:

The pAcGFP1-N1-Dsup was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14552).

Reference section:

Hashimoto, T., Horikawa, D.D., Saito, Y., Kuwahara, H., Kozuka-Hata, H., Shin-I, T., Minakuchi, Y., Ohishi, K., Motoyama, A., Aizu, T., Enomoto, A., Kondo, K., Tanaka, S., Hara, Y., Koshikawa, S., Sagara, H., Miura, T., Yokobori, S., Miyagawa, K., Suzuki, Y., Kubo, T., Oyama, M., Kohara, Y., Fujiyama, A., Arakawa, K., Katayama, T., Toyoda, A., Kunieda, T., Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Nat. Commun. 7: 12808 (2016). PMID 27649274. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Hashimoto, T., Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Nat. Commun. 7: 12808 (2016). PMID 27649274. [link to RRC of NBRP]