Resource data sheet


Expression vector of Ramazzottius varieornatus Dsup.

Catalog number RDB14551
Resource name pCXN2KS-Dsup
Clone info. Expression vector of Ramazzottius varieornatus Dsup under the CAG promoter for establishing a stable expression strain in mammalian cells.
Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pCXN2KS (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Ramazzottius varieornatus Dsup cDNA
Reference of nucleotide sequence LC050827 (DDBJ accession number)
Depositor Kunieda, Takekazu | Hashimoto, Takuma |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Hashimoto, T. et al. Nature Commu. 7, 12808, 2016).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14551 pCXN2KS-Dsup DNA solution

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Electronic file

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original Hashimoto, T., Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Nat. Commun. 7: 12808 (2016). PMID 27649274.