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pCXN2KS-Dsup (#RDB14551)

Expression vector of Ramazzottius varieornatus Dsup.

Clone info. Expression vector of Ramazzottius varieornatus Dsup under the CAG promoter for establishing a stable expression strain in mammalian cells.
Comment Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pCXN2KS (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Ramazzottius varieornatus Dsup cDNA
Reference of insert sequence LC050827 (DDBJ accession number)
Depositor|Developer Kunieda, Takekazu | Hashimoto, Takuma |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Hashimoto, T. et al. Nature Commu. 7, 12808, 2016).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hashimoto, T. et al. Nature Commu. 7, 12808, 2016)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14551 pCXN2KS-Dsup Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

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How to cite this biological resource

Materials & Methods section:

The pCXN2KS-Dsup was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14551).

Reference section:

Hashimoto, T., Horikawa, D.D., Saito, Y., Kuwahara, H., Kozuka-Hata, H., Shin-I, T., Minakuchi, Y., Ohishi, K., Motoyama, A., Aizu, T., Enomoto, A., Kondo, K., Tanaka, S., Hara, Y., Koshikawa, S., Sagara, H., Miura, T., Yokobori, S., Miyagawa, K., Suzuki, Y., Kubo, T., Oyama, M., Kohara, Y., Fujiyama, A., Arakawa, K., Katayama, T., Toyoda, A., Kunieda, T., Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Nat. Commun. 7: 12808 (2016). PMID 27649274. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

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References

Original, user report and related articles

original Hashimoto, T., Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Nat. Commun. 7: 12808 (2016). PMID 27649274. [link to RRC of NBRP]