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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCS2-OlCre (#RDB14533)

Expression vector of P1 phage Cre recombinase, codon-optimized for Oryzias latipes (japanese medaka).

Clone info. Expression vector of P1 phage Cre recombinase, codon-optimized for Oryzias latipes (japanese medaka) (GenBank accession number LC094445). DNA fragment 103 to 1221 nt of LC094445.1 is cloned.
Comment Known differences: T to C substitution at 1191 nt (Ala, synonym).
Vector backbone pCS2+ (Plasmid)
Gene/insert name P1 phage Cre genomic DNA
Reference of insert sequence LC094445 (DDBJ accession number)
Depositor|Developer Kinoshita, Masato |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Kishimoto, K. et al. Develop. Growth Differ. 58, 516-521, 2016)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kishimoto, K. et al. Develop. Growth Differ. 58, 516-521, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14533 pCS2-OlCre DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCS2-OlCre was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14533).

Reference section:

Kishimoto, K., Nakayama, M., Kinoshita, M., In vivo recombination efficiency of two site-specific recombination systems, VCre/VloxP and SCre/SloxP, in medaka (Oryzias latipes). Dev. Growth Differ. 58 (6): 516-521 (2016). PMID 27224047. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14533_A7Adp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F ()
Region: CMV IE94 promoter, OI Cre 5'
Sequence file: RDB14533_A7Ada.seq check
>D04061A2 A7Ad-1-pCMV IE94F_D03_12_ABI24.ab1
    1 CGGGTAAGCG TGCCTAATGG GAGGTCTATA TAAGCAATGC TCGTTTAGGG AACCGCCATT
   61 CTGCCTGGGG ACGTCGGAGC AAGCTTGATT TAGGTGACAC TATAGAATAC AAGCTACTTG
  121 TTCTTTTTGC AGGATCCCAT CGATTCGAAT TCACCATGGC GGAGGCCCTC GAGGCTAGCC
  181 CTCCTCCTAA GAGACCTAGA CTGGACTCTA GAATGGCGCG CCCCGGAGGT AGCGGTGGAA
  241 CTATGAGCAA CCTGCTGACC GTGCACCAGA ACCTGCCCGC CCTGCCCGTG GACGCCACCA
  301 GCGACGAGGT GAGGAAGAAC CTGATGGACA TGTTCAGGGA CAGGCAGGCC TTCAGCGAGC
  361 ACACCTGGAA GATGCTGCTG AGCGTGTGCA GGAGCTGGGC CGCCTGGTGC AAGCTGAACA
  421 ACAGGAAGTG GTTCCCCGCC GAGCCCGAGG ACGTGAGGGA CTACCTGCTG TACCTGCAGG
  481 CCAGGGGCCT GGCCGTGAAG ACCATCCAGC AGCACCTGGG CCAGCTGAAC ATGCTGCACA
  541 GGAGGAGCGG CCTGCCCAGG CCCAGCGACA GCAACGCCGT GAGCCTGGTG ATGAGGAGGA
  601 TCAGGAAGGA GAACGTGGAC GCCGGCGAGA GGGCCAAGCA GGCCCTGGCC TTCGAGAGGA
  661 CCGACTTCGA CCAGGTGAGG AGCCTGATGG AGAACAGCGA CAGGTGCCAG GACATCATGA
  721 ACCTGGTCTT TCCTGGCCAT CGGCTACCAC CACCCTGCTG AGGGATCGCC CGGAGATCCG
781 GCCCAAGA
//
Primer: pAxCALNL_F1 ()
Region: SV40 poly(A) signal, OI Cre 3'
Sequence file: RDB14533_A7Adb.seq check
>D04061A2_A7Ad_1_pAxCALNL_F1_F06_18_ABI24.ab1
    1 CCAAGGTAAA CCCTTAATCA TGTCTGGATC TACGTAATAC GACTCACTAT AGTCTAGATG
   61 CATGCTCGAC TCAGTCGCCG TCCTCCAGCA GCCTCACCAT GGCGCCGGTC TCGCTGTCCA
  121 GGTTCCTGAT GTAGTTCATC ACGATGTTCA CGTTGGTCCA GCCGCCGGCC TGCATGATCT
  181 CGGGGATGCT CACGCCGGCC CTGGCCATGT CCCTGGCGGC GCCCACCCTG GCGCTGTGGC
  241 CGCTCCAGGC CAGGTACCTC TGGCCGCTGT CGTCCTTGGC GCCGTAGATC AGCCTGTGGG
  301 TGGCCTCGAA GATGCCCTCC AGGGCCCTGG TGCTCAGCTG GCTGGTGGCG CTGGGGGCGG
  361 CCACGCCGTT CTTCCTCACC CTGCAGAACA GGTAGTTGTT GGGGTCGTCG GCCACGCCGC
  421 TCACGCTGAT CCACCTCTCC ACCAGCTTGG TCACGCCCAG GCTCAGGGCC TTCTCCACGC
  481 CGGCGGTGCT CACCAGGGTC TTGGTCCTGC CGATGTGGAT CAGCATCCTG CCGCCGTCGG
  541 TCCTGCTGAT GTCCTTCACC CTGATCCTGG CGATCTCGGC GATCCTCAGC AGGGTGTTGT
  601 AGGCGATGCC CAGGAAGGCC AGGTTCCTGA TGTCCTGGCA CCTGTCGCTG TTCTCCATCA
  661 GGCTCCTCAC CTGGTCGAAG TCGGTCCTCT CGAAGGCCAG GGCCTGCTTG GCCCTCTCGC
  721 CGGCGTCCAC GTTCTCCTTC CTGATCCTCC TCATCACCAG GCTCACGGCG TTGCTGTCGC
  781 TGGGCCTGGG CAGGCCGCTC CTCCTGTGCA GCATGTTCAG CTGGCCCAGG TGCTGCTGGA
  841 TGGTCTTCAC GGCCAGGCCC CTGGCCTGCA GGTACAGCAG GTAGTCCCTC ACGTCCTCGG
  901 GCTCGGCGGG GAACCACTTT CCTGTTGTTC AGCTTGCACC AGGCGGCCCA GCTCCTGCAC
  961 ACGCTCAGCA GCATCTTCCA GTGTGCTCGC TGAAAGGCCT GCCTGTCCCT TGAACATGTT
 1021 CCAATCAAGT TTCTTCTCAC TCGTCGCTGG TGGCGTCCAC GGCAGGCGGG CCAGGGTTCC
1081 TGGTGCACGA TCAGCAGATG CTCATTAGTC CACCGCTACT TCAGGCGCTC TAAGTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kishimoto, K., In vivo recombination efficiency of two site-specific recombination systems, VCre/VloxP and SCre/SloxP, in medaka (Oryzias latipes). Dev. Growth Differ. 58 (6): 516-521 (2016). PMID 27224047. [link to RRC of NBRP]