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pCS2-OlCre

Expression vector of P1 phage Cre recombinase, codon-optimized for Oryzias latipes (japanese medaka).

Catalog number RDB14533
Resource name pCS2-OlCre
Clone info. Expression vector of P1 phage Cre recombinase, codon-optimized for Oryzias latipes (japanese medaka) (GenBank accession number LC094445). DNA fragment 103 to 1221 nt of LC094445.1 is cloned.
Known differences: T to C substitution at 1191 nt (Ala, synonym).
Vector backbone pCS2+ (Plasmid)
Gene/insert name P1 phage Cre genomic DNA
Reference of nucleotide sequence LC094445 (DDBJ accession number)
Depositor Kinoshita, Masato |
 
Other clones in our bank P1 phage cre |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Kishimoto, K. et al. Develop. Growth Differ. 58, 516-521, 2016)
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RDB14533 pCS2-OlCre DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Kishimoto, K., In vivo recombination efficiency of two site-specific recombination systems, VCre/VloxP and SCre/SloxP, in medaka (Oryzias latipes). Dev. Growth Differ. 58 (6): 516-521 (2016). PMID 27224047.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Cre-loxP and FLP-FRT System (English text)
Featured content Control Vectors (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14533_A7Adp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCMV-IE94_F RDB14533_A7Ada.seq
>D04061A2 A7Ad-1-pCMV IE94F_D03_12_ABI24.ab1
    1 CGGGTAAGCG TGCCTAATGG GAGGTCTATA TAAGCAATGC TCGTTTAGGG AACCGCCATT
   61 CTGCCTGGGG ACGTCGGAGC AAGCTTGATT TAGGTGACAC TATAGAATAC AAGCTACTTG
  121 TTCTTTTTGC AGGATCCCAT CGATTCGAAT TCACCATGGC GGAGGCCCTC GAGGCTAGCC
  181 CTCCTCCTAA GAGACCTAGA CTGGACTCTA GAATGGCGCG CCCCGGAGGT AGCGGTGGAA
  241 CTATGAGCAA CCTGCTGACC GTGCACCAGA ACCTGCCCGC CCTGCCCGTG GACGCCACCA
  301 GCGACGAGGT GAGGAAGAAC CTGATGGACA TGTTCAGGGA CAGGCAGGCC TTCAGCGAGC
  361 ACACCTGGAA GATGCTGCTG AGCGTGTGCA GGAGCTGGGC CGCCTGGTGC AAGCTGAACA
  421 ACAGGAAGTG GTTCCCCGCC GAGCCCGAGG ACGTGAGGGA CTACCTGCTG TACCTGCAGG
  481 CCAGGGGCCT GGCCGTGAAG ACCATCCAGC AGCACCTGGG CCAGCTGAAC ATGCTGCACA
  541 GGAGGAGCGG CCTGCCCAGG CCCAGCGACA GCAACGCCGT GAGCCTGGTG ATGAGGAGGA
  601 TCAGGAAGGA GAACGTGGAC GCCGGCGAGA GGGCCAAGCA GGCCCTGGCC TTCGAGAGGA
  661 CCGACTTCGA CCAGGTGAGG AGCCTGATGG AGAACAGCGA CAGGTGCCAG GACATCATGA
  721 ACCTGGTCTT TCCTGGCCAT CGGCTACCAC CACCCTGCTG AGGGATCGCC CGGAGATCCG
781 GCCCAAGA
//
Primer: pAxCALNL_F1 RDB14533_A7Adb.seq
>D04061A2_A7Ad_1_pAxCALNL_F1_F06_18_ABI24.ab1
    1 CCAAGGTAAA CCCTTAATCA TGTCTGGATC TACGTAATAC GACTCACTAT AGTCTAGATG
   61 CATGCTCGAC TCAGTCGCCG TCCTCCAGCA GCCTCACCAT GGCGCCGGTC TCGCTGTCCA
  121 GGTTCCTGAT GTAGTTCATC ACGATGTTCA CGTTGGTCCA GCCGCCGGCC TGCATGATCT
  181 CGGGGATGCT CACGCCGGCC CTGGCCATGT CCCTGGCGGC GCCCACCCTG GCGCTGTGGC
  241 CGCTCCAGGC CAGGTACCTC TGGCCGCTGT CGTCCTTGGC GCCGTAGATC AGCCTGTGGG
  301 TGGCCTCGAA GATGCCCTCC AGGGCCCTGG TGCTCAGCTG GCTGGTGGCG CTGGGGGCGG
  361 CCACGCCGTT CTTCCTCACC CTGCAGAACA GGTAGTTGTT GGGGTCGTCG GCCACGCCGC
  421 TCACGCTGAT CCACCTCTCC ACCAGCTTGG TCACGCCCAG GCTCAGGGCC TTCTCCACGC
  481 CGGCGGTGCT CACCAGGGTC TTGGTCCTGC CGATGTGGAT CAGCATCCTG CCGCCGTCGG
  541 TCCTGCTGAT GTCCTTCACC CTGATCCTGG CGATCTCGGC GATCCTCAGC AGGGTGTTGT
  601 AGGCGATGCC CAGGAAGGCC AGGTTCCTGA TGTCCTGGCA CCTGTCGCTG TTCTCCATCA
  661 GGCTCCTCAC CTGGTCGAAG TCGGTCCTCT CGAAGGCCAG GGCCTGCTTG GCCCTCTCGC
  721 CGGCGTCCAC GTTCTCCTTC CTGATCCTCC TCATCACCAG GCTCACGGCG TTGCTGTCGC
  781 TGGGCCTGGG CAGGCCGCTC CTCCTGTGCA GCATGTTCAG CTGGCCCAGG TGCTGCTGGA
  841 TGGTCTTCAC GGCCAGGCCC CTGGCCTGCA GGTACAGCAG GTAGTCCCTC ACGTCCTCGG
  901 GCTCGGCGGG GAACCACTTT CCTGTTGTTC AGCTTGCACC AGGCGGCCCA GCTCCTGCAC
  961 ACGCTCAGCA GCATCTTCCA GTGTGCTCGC TGAAAGGCCT GCCTGTCCCT TGAACATGTT
 1021 CCAATCAAGT TTCTTCTCAC TCGTCGCTGG TGGCGTCCAC GGCAGGCGGG CCAGGGTTCC
1081 TGGTGCACGA TCAGCAGATG CTCATTAGTC CACCGCTACT TCAGGCGCTC TAAGTG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kishimoto, K., In vivo recombination efficiency of two site-specific recombination systems, VCre/VloxP and SCre/SloxP, in medaka (Oryzias latipes). Dev. Growth Differ. 58 (6): 516-521 (2016). PMID 27224047.

2019.08.05

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