Expression vector of S. cerevisiae SED5 tagged with N-terminal mRFP with the TRP1 marker.
Alternative name | pRS314-pTDH3-mRFP-SED5-tCMK1 |
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Clone info. | Single copy (YCp) expression vector of S. cerevisiae SED5 tagged with N-terminal mRFP under the TDH3 promoter and CMK1 terminator with the TRP1 marker. |
Comment | Expression was confirmed by the depositor with fluorescence microscopy. |
Vector backbone | pRS314 (plasmid) |
Selectable markers | Amp^r (E. coli), TRP1 (S. cerevisiae). |
Gene/insert name | S. cerevisiae SED5 genomic DNA Discosoma sp. RFP cDNA |
Depositor|Developer | Shimizu, Shigeomi | Yamaguchi, Hirofumi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Yamaguchi, H. et al. EMBO J. 35 (18), 1991-2007, 2016) Additional terms and conditions: The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
Remarks | This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yamaguchi, H. et al. EMBO J. 35 (18), 1991-2007, 2016)。 MTAに書く付加的使用条件: 非営利機関の非営利学術研究に限って提供する。 |
備考 | Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB14527 | pHYA1166 | Under QC test. Please contact us. | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pHYA1166 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14527). |
Reference section:
Yamaguchi, H., Arakawa, S., Kanaseki, T., Miyatsuka, T., Fujitani, Y., Watada, H., Tsujimoto, Y., Shimizu, S., Golgi membrane-associated degradation pathway in yeast and mammals. EMBO J. 35 (18): 1991-2007 (2016). PMID 27511903. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Yamaguchi, H., Golgi membrane-associated degradation pathway in yeast and mammals. EMBO J. 35 (18): 1991-2007 (2016). PMID 27511903. [link to RRC of NBRP] |
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