Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pHYA1166 (#RDB14527)

Expression vector of S. cerevisiae SED5 tagged with N-terminal mRFP with the TRP1 marker.

Alternative name pRS314-pTDH3-mRFP-SED5-tCMK1
Clone info. Single copy (YCp) expression vector of S. cerevisiae SED5 tagged with N-terminal mRFP under the TDH3 promoter and CMK1 terminator with the TRP1 marker.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1 (S. cerevisiae).
Gene/insert name S. cerevisiae SED5 genomic DNA Discosoma sp. RFP cDNA
Depositor|Developer Shimizu, Shigeomi | Yamaguchi, Hirofumi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Yamaguchi, H. et al. EMBO J. 35 (18), 1991-2007, 2016)
Additional terms and conditions:
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yamaguchi, H. et al. EMBO J. 35 (18), 1991-2007, 2016)。
MTAに書く付加的使用条件：
非営利機関の非営利学術研究に限って提供する。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14527 pHYA1166 Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pHYA1166 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14527).

Reference section:

Yamaguchi, H., Arakawa, S., Kanaseki, T., Miyatsuka, T., Fujitani, Y., Watada, H., Tsujimoto, Y., Shimizu, S., Golgi membrane-associated degradation pathway in yeast and mammals. EMBO J. 35 (18): 1991-2007 (2016). PMID 27511903. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Yamaguchi, H., Golgi membrane-associated degradation pathway in yeast and mammals. EMBO J. 35 (18): 1991-2007 (2016). PMID 27511903. [link to RRC of NBRP]