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pHYA1133

Expression vector of S. cerevisiae PEP4 tagged with C-terminal mRFP with the TRP1 marker.

Catalog number RDB14525
Resource name pHYA1133
Alternative name pRS314-pTDH3-PEP4-mRFP-tCMK1
Clone info. Single copy (YCp) expression vector of S. cerevisiae PEP4 tagged with C-terminal mRFP under the TDH3 promoter and CMK1 terminator with the TRP1 marker.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1 (S. cerevisiae).
Gene/insert name S. cerevisiae PEP4 genomic DNA Discosoma sp. RFP cDNA
Depositor|Developer Shimizu, Shigeomi | Yamaguchi, Hirofumi |
Other clones in our bank
External Database
S. cerevisiae PEP4 Discosoma sp. RFP
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Yamaguchi, H. et al. EMBO J. 35 (18), 1991-2007, 2016)
          Additional terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yamaguchi, H. et al. EMBO J. 35 (18), 1991-2007, 2016)。
          付加的提供条件 非営利機関の非営利学術研究に限って提供する。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB14525 pHYA1133 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Yamaguchi, H., Golgi membrane-associated degradation pathway in yeast and mammals. EMBO J. 35 (18): 1991-2007 (2016). PMID 27511903.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Yamaguchi, H., Golgi membrane-associated degradation pathway in yeast and mammals. EMBO J. 35 (18): 1991-2007 (2016). PMID 27511903.

          2022.05.17

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