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pHYA1127 (#RDB14524)

Expression vector of S. cerevisiae GOS1 tagged with N-terminal GFP with the URA3 marker.

Alternative name pRS316-pTDH3-GFP-GOS1-tCMK1
Clone info. Single copy (YCp) expression vector of S. cerevisiae GOS1 tagged with N-terminal GFP under the TDH3 promoter and CMK1 terminator with the URA3 marker.
Comment Expression was confirmed by the depositor with fluorescence microscopy and western blotting.
Vector backbone pRS316 (plasmid)
Selectable markers Amp^r (E. coli), URA3 (S. cerevisiae).
Gene/insert name S. cerevisiae GOS1 genomic DNA Aequorea victoria GFP cDNA
Depositor|Developer Shimizu, Shigeomi | Yamaguchi, Hirofumi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Yamaguchi, H. et al. EMBO J. 35 (18), 1991-2007, 2016)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yamaguchi, H. et al. EMBO J. 35 (18), 1991-2007, 2016)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14524 pHYA1127 Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

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How to cite this biological resource

Materials & Methods section:

The pHYA1127 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14524).

Reference section:

Yamaguchi, H., Arakawa, S., Kanaseki, T., Miyatsuka, T., Fujitani, Y., Watada, H., Tsujimoto, Y., Shimizu, S., Golgi membrane-associated degradation pathway in yeast and mammals. EMBO J. 35 (18): 1991-2007 (2016). PMID 27511903. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

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References

Original, user report and related articles

original Yamaguchi, H., Golgi membrane-associated degradation pathway in yeast and mammals. EMBO J. 35 (18): 1991-2007 (2016). PMID 27511903. [link to RRC of NBRP]