Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-IRES-GFP_CA-Akt(E40K)

Retroviral expression vector of constitutively active Akt(E40K)

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested. Additional terms and conditions: Regarding CA-Akt E40K: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for CA-Akt E40K (Arimura, Y., J. Biol. Chem. 279 (12), 11408-11416, 2004) and an acknowledgment to Dr. Junji Yagi of Tokyo Women's Meical University are requested. Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 （Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016） を引用し、寄託者への謝辞の表明を必要とする。 付加的提供条件： CA-Akt E40Kについて：利用者は、研究成果の公表にあたって CA-Akt E40Kの文献 （Arimura, Y., J. Biol. Chem. 279 (12), 11408-11416, 2004) を引用し、東京女子医科大学八木淳二先生への謝辞の表明を必要とする。 pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14514_A6Lep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' Sequence file: RDB14514_A6Lea.seq
>D04073B5_A6Le_1_pBABE5'_H01_22_ABI24.ab1     1 GCCTTAATGG CATATGAGAT CTTATATGGG GCACCCCCGC CCCTTGTAAA CTTCCCTGAC    61 CCTGACATGA CAAGAGTTAC TAACAGCCCC TCTCTCCAAG CTCACTTACA GGCTCTCTAC   121 TTAGTCCAGC ACGAAGTCTG GAGACCTCTG GCGGCAGCCT ACCAAGAACA ACTGGACCGA   181 CCGGTGGTAC CTCACCCTTA CCGAGTCGGC GACACAGTGT GGGTCCGCCG ACACCAGACT   241 AAGAACCTAG AACCTCGCTG GAAAGGACCT TACACAGTCC TGCTGACCAC CCCCACCGCC   301 CTCAAAGTAG ACGGCATCGC AGCTTGGATA CACGCCGCCC ACGTGAAGGC TGCCGACCCC   361 GGGGGTGGAC CATCCTCTAG ACTGCCGGAT CCGGCCACGG ATACCATGAA CGACGTAGCC   421 ATTGTGAAGG AGGGCTGGCT GCACAAACGA GGGGAATATA TTAAAACCTG GCGGCCACGC   481 TACTTCCTCC TCAAGAACGA TGGCACCTTT ATTGGCTACA AGAAACGGCC TCAGGATGTG   541 GATCAGCGAG AGTCCCCACT CAACAACTTC TCAGTGGCAC AATGCCAGCT GATGAAGACA   601 GAGCGGCCAA GGCCCAACAC CTTTATCATC CGCTGCCTGC AGTGGACCAC AGTCATTGAG   661 CGCACCTTCC ATGTGGAAAC GCCTGAGGAG CGGGAAGAAT GGGCCACCGC CATTCAGACT   721 GTGGCAGATG GACTCAAGAG GCAGGAAGAA GAGACGATGG ACTTCCGATC AGGCTCACCC   781 AGTGACAACT CAGGGGCTGA AGAGATGGAG GTGTCCCTGG CCAAGCCCAA GCACCGTGTG   841 ACCATGAACG AGTTTGAGTA CCTGAAGCTA CTGGGCAAGG GCACCTTTGG GAAGGTGATT   901 CTGGTGAAAG AGAAGGCCAC AGGCCGCTAC TATGCCATGA AGATCCTCAA GAAGGAGGTC   961 ATCGTCGCCC AAGGATGAGG TTGCCCACAC GCTTACTGAG AACCGTGTCC TGCAGAACTC  1021 TAGGCATCCC TTTCCTTTAC GGCCCTCAAG TACTCATTCC AGACCCACGA CCGCCTCTGC  1081 TTTGTCATGA GTATGCCCAA CGGGGCGAGC TCTTCGTCAA CTGTCTCGAG AGCGTGTGTT 1141 TCCTTCGGAG GAACCGGGGT C //
Primer: IRES-R Sequence file: RDB14514_A6Leb.seq
>D04073B5_A6Le_1_IRES-R_F03_18_ABI24.ab1     1 GCTCAGCGGC TTCGGCAGTA CGTTAGGGGG GGGGGAGGGA GAGGGGGGGG GGGGGCGGAA    61 TTTACGTAGC GGCCGCGGCG CGCCGGCCCT CGAGGCCTGC AGGAATTCGG CCTCAGGCTG   121 TGCCACTGGC TGAGTAGGAG AACTGGGGGA AGTGCGGCCT CCGCTCACTG TCCACACACT   181 CCATGCTGTC ATCTTGATCA GGCGGCGTGA TGGTGATCAT CTGAGCTGTG AACTCCTCAT   241 CGAAATACCT GGTGTCAGTC TCAGAGGTGA CCTGGGGCTT GAAAGGTGGG CTCAGCTTCT   301 TCTCATACAC ATCCTGCCAC ACGATGTTGG CAAAGAACCG GTGCTGCATG ATCTCCTTGG   361 CATCCTCGGA GCCCCCACCG AGCCTCTGTG TAGGGTCCTT CTTGAGCAGC CCGGAGAGCA   421 GGGACTTGGC CTCAGGGCCG AGTGTGCGCG GGAAGCGGAT CTCCTCCATG AGGATCAGCT   481 CGAACAGCTT CTCGTGGTCC TGGTTGTAGA AGGGCAGGCG GCCACACATC ATCTCGTACA   541 TGACCACGCC CAGCCCCCAC CAGTCCACTG CACGGCCGTA GTCGTTGTCC TCCAGCACCT   601 CAGGGGCCAG GTACTCCGGC GTTCCGCAGA ATGTCCTCAT AGTGGCACCG TCCTTGATCC   661 CCTCCTTGCA CAGCCCGAAG TCCGTTATCT TGATGTGCCC GTCCTTGTCC AGCATGAGGT   721 TCTCCAGCTT CAGGTCCCGG TACACCACGT TCTTCTCGGA GTGCAAGTAG TCCAGGGCAG   781 ACACAATCTC CGCACCATAG AAGCGGGCCC GGTCCTCGGA GAACACACGC TCTCGAGACA   841 GGTGGAAGAA GAGCTCGCCC CCGTTGGCAT ACTCCATGAC AAAGCAGAGG CGGTCGTGGG   901 TCTGGAAATG AGTACTTGAG GGCCGTAAGG GAAGGGATGC CTAGAGTTCT GCAGGACACG   961 GGTTCTCAGT AAGCGTGTGG GCAACCTCAT CCTTGGCGAC GATGACCTCT TCTGAGATCT  1021 TCATTGCATA GTAGCGGCTG TGCTTCTCTT TCACAGATCA CTTACTAAAG TGCCCTGGCC 1081 AGTAGCTCAG TACTCACTCG ATCATGTCAC ACGGTTGCTT GGGCCTG //
Primer: IRES-F Sequence file: RDB14514_A6Lec.seq
>D04073B5_A6Le_1_IRES-F_H12_24_ABI24.ab1     1 GGGATTCCTT TATGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG    61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC   121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC   181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG   241 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG   301 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC   361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC   421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG   481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC   541 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC   601 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG   661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG   721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG   781 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGGCCGCCA   841 GCACAGTGGT CGACGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA GGGGGGAATG   901 AAAGACCCCA CCTGTAGGTT TGGGCAAGCT AGCTTAAGTA ACGCCCATTT TGCAAGCATG   961 GGAAAAAATA CATAACTGGA GAATAGAGAA GTTCAGATCA AGGGTTCAAG GAACAGAATG  1021 GAACAGCCTG AATAATGGGT CCAAACCAGG AATATCTGGT TGATAAGCAA GTTTCTTGCC 1081 CCGGCTTCAA AGTCCAGAAC GGATTGAACA GCCTTGGAAT ATGGGGCGCC CAAACAG //
Primer: Amp_R Sequence file: RDB14514_A6Led.seq
>D04073B5_A6Le_1_Amp_R_G03_21_ABI24.ab1     1 CTTCGTTTTC ATATTATTGA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT    61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC   121 ACCTGCATAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT   181 TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAAAAGT TCAGATCAAG GTCAGGAACA   241 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC   301 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC   361 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG   421 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT   481 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC   541 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATTGACTGA   601 GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT   661 CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA   721 TTTGGGGGCT CGTCCGGGAT CGGGAGACCC CTGCCCAGGG ACCACCGACC CACCACCGGG   781 AGGTAAGCTG GCCAGCAACT TATCTGTGTC TGTCCGATTG TCTAGTGTCT ATGACTGATT   841 TTATGCGCCT GCGTCGGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG   901 GAACTGACGA GTTCGGAACA CCCGGCCGCA ACCCTGGGAG ACGTCCCAGG GACTTCGGGG   961 GCCGTTTTTG TGGCCCGACC TGAGTCCAAA AATCCCGATC GTTTTGGACT CTTTGGTGCA  1021 CCCCCCCTTA GAGGAGGATA TGTGGATCTG GTAGGAGACG AGACCTAAAC AGGTCCGCTT  1081 CGTCCTGAGT CTGCTTCGGC TTTGGAACGG AAGCCGCGAC CGCGCGATCT TTGTTCTTTG 1141 ACTTGA //
Primer: SV40pA3'element Reverse Sequence file: RDB14514_A6Lee.seq
>D04073B5_A6Le_1_SV40pA3'element Reverse_H03_24_ABI24.ab1     1 GATGGTAGGG GGGCGGAGCT ATGGAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC    61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA   121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG   181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG   241 TTGGCCGATT CATTAATGCA ACTATGGCCA TTTAATGTAA ATACTTAAGA AAAAAAACCA   301 AATTAATTTT GATACATGCT GCATGTGAAA GACCCCCGCT GACGGGTAGT CAATCACTCA   361 GAGGAGACCC TCCCAAGGAA CAGCGAGACC ACAAGTCGGA TGCAACTGCA AGAGGGTTTA   421 TTGGATACAC GGGTACCCGG GCGACTCAGT CAATCGGAGG ACTGGCGCCC CGAGTGAGGG   481 GTTGTGGGCT CTTTTATTGA GCTCGGGGAG CAGAAGCGCG CGAACAGAAG CGAGAAGCGA   541 ACTGATTGGT TAGTTCAAAT AAGGCACAGG GTCATTTCAG GTCCTTGGGG CACCCTGGAA   601 ACATCTGATG GTTCTCTAGA AACTGCTGAG GGCTGGACCG CATCTGGGGA CCATCTGTTC   661 TTGGCCCTGA GCCGGGGCAG GAACTGCTTA CCACAGATAT CCTGTTTGGC CCATATTCAG   721 CTGTTCCATC TGTTCTTGGC CCTGAGCCGG GGCAGGAACT GCTTACCACA GATATCCTGT   781 TTGGCCCATA TTCAGCTGTT CCATCTGTTC CTGACCTTGA TCTGAACTTC TCTATTCTCA   841 GTTATGTATT TTTTCCATGC CTTGCAAAAT GGCGTTACTT AAGCTAGCTT GCCAAACCTA   901 CAGGTGGGGT CTTTCATTCC CCCCCTTTTT TCTGGAGACT AAATAAAATC TTTTATTTTA   961 TCGTCGACCA CTGTGCTGGG CGGCCGGGCC GCTTTACTTG TACAGCTCGT CCATGCCCGA  1021 GAGTGATCCC GGCGCGTCAC GACTCCAGCA GGAACAATGT GATCGCGCTT CTCGTTGGGT 1081 CTTTGCTCAA GTCGGACTGG GTGCCTCCAA GGTTAGGTTG GGT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl