Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-IRES-GFP_CA-Akt(E40K) (#RDB14514)

Retroviral expression vector of constitutively active Akt(E40K)

Clone info. Retroviral expression vector of constitutively active Akt(E40K), cloned into the pMXs-IRES-GFP Refer Fig.2 of Haniuda, K., Nat. Immunol., 17 (9): 1109-1117 (2016).
Comment Expression was confirmed by the depositor with flow cytometry. Sequence primer: 5term-GGTGGACCATCCTCTAGACT-3term.
Vector backbone pMXs-IRES-GFP (Plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name mouse Akt1 cDNA
Depositor|Developer Kitamura, Daisuke |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions:
Regarding CA-Akt E40K: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for CA-Akt E40K (Arimura, Y., J. Biol. Chem. 279 (12), 11408-11416, 2004) and an acknowledgment to Dr. Junji Yagi of Tokyo Women's Meical University are requested.
Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016） を引用し、寄託者への謝辞の表明を必要とする。
MTAに書く付加的使用条件：
CA-Akt E40Kについて：利用者は、研究成果の公表にあたって CA-Akt E40Kの文献 （Arimura, Y., J. Biol. Chem. 279 (12), 11408-11416, 2004) を引用し、東京女子医科大学八木淳二先生への謝辞の表明を必要とする。
pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14514 pMXs-IRES-GFP_CA-Akt(E40K) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-IRES-GFP_CA-Akt(E40K) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14514).

Reference section:

Haniuda, K., Fukao, S., Kodama, T., Hasegawa, H., Kitamura, D., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14514_A6Lep1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5'
Sequence file: RDB14514_A6Lea.seq check
>D04073B5_A6Le_1_pBABE5'_H01_22_ABI24.ab1
    1 GCCTTAATGG CATATGAGAT CTTATATGGG GCACCCCCGC CCCTTGTAAA CTTCCCTGAC
   61 CCTGACATGA CAAGAGTTAC TAACAGCCCC TCTCTCCAAG CTCACTTACA GGCTCTCTAC
  121 TTAGTCCAGC ACGAAGTCTG GAGACCTCTG GCGGCAGCCT ACCAAGAACA ACTGGACCGA
  181 CCGGTGGTAC CTCACCCTTA CCGAGTCGGC GACACAGTGT GGGTCCGCCG ACACCAGACT
  241 AAGAACCTAG AACCTCGCTG GAAAGGACCT TACACAGTCC TGCTGACCAC CCCCACCGCC
  301 CTCAAAGTAG ACGGCATCGC AGCTTGGATA CACGCCGCCC ACGTGAAGGC TGCCGACCCC
  361 GGGGGTGGAC CATCCTCTAG ACTGCCGGAT CCGGCCACGG ATACCATGAA CGACGTAGCC
  421 ATTGTGAAGG AGGGCTGGCT GCACAAACGA GGGGAATATA TTAAAACCTG GCGGCCACGC
  481 TACTTCCTCC TCAAGAACGA TGGCACCTTT ATTGGCTACA AGAAACGGCC TCAGGATGTG
  541 GATCAGCGAG AGTCCCCACT CAACAACTTC TCAGTGGCAC AATGCCAGCT GATGAAGACA
  601 GAGCGGCCAA GGCCCAACAC CTTTATCATC CGCTGCCTGC AGTGGACCAC AGTCATTGAG
  661 CGCACCTTCC ATGTGGAAAC GCCTGAGGAG CGGGAAGAAT GGGCCACCGC CATTCAGACT
  721 GTGGCAGATG GACTCAAGAG GCAGGAAGAA GAGACGATGG ACTTCCGATC AGGCTCACCC
  781 AGTGACAACT CAGGGGCTGA AGAGATGGAG GTGTCCCTGG CCAAGCCCAA GCACCGTGTG
  841 ACCATGAACG AGTTTGAGTA CCTGAAGCTA CTGGGCAAGG GCACCTTTGG GAAGGTGATT
  901 CTGGTGAAAG AGAAGGCCAC AGGCCGCTAC TATGCCATGA AGATCCTCAA GAAGGAGGTC
  961 ATCGTCGCCC AAGGATGAGG TTGCCCACAC GCTTACTGAG AACCGTGTCC TGCAGAACTC
 1021 TAGGCATCCC TTTCCTTTAC GGCCCTCAAG TACTCATTCC AGACCCACGA CCGCCTCTGC
 1081 TTTGTCATGA GTATGCCCAA CGGGGCGAGC TCTTCGTCAA CTGTCTCGAG AGCGTGTGTT
1141 TCCTTCGGAG GAACCGGGGT C
//
Primer: IRES-R
Sequence file: RDB14514_A6Leb.seq check
>D04073B5_A6Le_1_IRES-R_F03_18_ABI24.ab1
    1 GCTCAGCGGC TTCGGCAGTA CGTTAGGGGG GGGGGAGGGA GAGGGGGGGG GGGGGCGGAA
   61 TTTACGTAGC GGCCGCGGCG CGCCGGCCCT CGAGGCCTGC AGGAATTCGG CCTCAGGCTG
  121 TGCCACTGGC TGAGTAGGAG AACTGGGGGA AGTGCGGCCT CCGCTCACTG TCCACACACT
  181 CCATGCTGTC ATCTTGATCA GGCGGCGTGA TGGTGATCAT CTGAGCTGTG AACTCCTCAT
  241 CGAAATACCT GGTGTCAGTC TCAGAGGTGA CCTGGGGCTT GAAAGGTGGG CTCAGCTTCT
  301 TCTCATACAC ATCCTGCCAC ACGATGTTGG CAAAGAACCG GTGCTGCATG ATCTCCTTGG
  361 CATCCTCGGA GCCCCCACCG AGCCTCTGTG TAGGGTCCTT CTTGAGCAGC CCGGAGAGCA
  421 GGGACTTGGC CTCAGGGCCG AGTGTGCGCG GGAAGCGGAT CTCCTCCATG AGGATCAGCT
  481 CGAACAGCTT CTCGTGGTCC TGGTTGTAGA AGGGCAGGCG GCCACACATC ATCTCGTACA
  541 TGACCACGCC CAGCCCCCAC CAGTCCACTG CACGGCCGTA GTCGTTGTCC TCCAGCACCT
  601 CAGGGGCCAG GTACTCCGGC GTTCCGCAGA ATGTCCTCAT AGTGGCACCG TCCTTGATCC
  661 CCTCCTTGCA CAGCCCGAAG TCCGTTATCT TGATGTGCCC GTCCTTGTCC AGCATGAGGT
  721 TCTCCAGCTT CAGGTCCCGG TACACCACGT TCTTCTCGGA GTGCAAGTAG TCCAGGGCAG
  781 ACACAATCTC CGCACCATAG AAGCGGGCCC GGTCCTCGGA GAACACACGC TCTCGAGACA
  841 GGTGGAAGAA GAGCTCGCCC CCGTTGGCAT ACTCCATGAC AAAGCAGAGG CGGTCGTGGG
  901 TCTGGAAATG AGTACTTGAG GGCCGTAAGG GAAGGGATGC CTAGAGTTCT GCAGGACACG
  961 GGTTCTCAGT AAGCGTGTGG GCAACCTCAT CCTTGGCGAC GATGACCTCT TCTGAGATCT
 1021 TCATTGCATA GTAGCGGCTG TGCTTCTCTT TCACAGATCA CTTACTAAAG TGCCCTGGCC
1081 AGTAGCTCAG TACTCACTCG ATCATGTCAC ACGGTTGCTT GGGCCTG
//
Primer: IRES-F
Sequence file: RDB14514_A6Lec.seq check
>D04073B5_A6Le_1_IRES-F_H12_24_ABI24.ab1
    1 GGGATTCCTT TATGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG
   61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC
  121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
  181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
  241 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
  301 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
  361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
  421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
  481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
  541 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC
  601 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG
  661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG
  721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
  781 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGGCCGCCA
  841 GCACAGTGGT CGACGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA GGGGGGAATG
  901 AAAGACCCCA CCTGTAGGTT TGGGCAAGCT AGCTTAAGTA ACGCCCATTT TGCAAGCATG
  961 GGAAAAAATA CATAACTGGA GAATAGAGAA GTTCAGATCA AGGGTTCAAG GAACAGAATG
 1021 GAACAGCCTG AATAATGGGT CCAAACCAGG AATATCTGGT TGATAAGCAA GTTTCTTGCC
1081 CCGGCTTCAA AGTCCAGAAC GGATTGAACA GCCTTGGAAT ATGGGGCGCC CAAACAG
//
Primer: Amp_R
Sequence file: RDB14514_A6Led.seq check
>D04073B5_A6Le_1_Amp_R_G03_21_ABI24.ab1
    1 CTTCGTTTTC ATATTATTGA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
   61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
  121 ACCTGCATAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT
  181 TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAAAAGT TCAGATCAAG GTCAGGAACA
  241 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC
  301 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC
  361 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG
  421 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT
  481 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC
  541 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATTGACTGA
  601 GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT
  661 CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA
  721 TTTGGGGGCT CGTCCGGGAT CGGGAGACCC CTGCCCAGGG ACCACCGACC CACCACCGGG
  781 AGGTAAGCTG GCCAGCAACT TATCTGTGTC TGTCCGATTG TCTAGTGTCT ATGACTGATT
  841 TTATGCGCCT GCGTCGGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG
  901 GAACTGACGA GTTCGGAACA CCCGGCCGCA ACCCTGGGAG ACGTCCCAGG GACTTCGGGG
  961 GCCGTTTTTG TGGCCCGACC TGAGTCCAAA AATCCCGATC GTTTTGGACT CTTTGGTGCA
 1021 CCCCCCCTTA GAGGAGGATA TGTGGATCTG GTAGGAGACG AGACCTAAAC AGGTCCGCTT
 1081 CGTCCTGAGT CTGCTTCGGC TTTGGAACGG AAGCCGCGAC CGCGCGATCT TTGTTCTTTG
1141 ACTTGA
//
Primer: SV40pA3'element Reverse
Sequence file: RDB14514_A6Lee.seq check
>D04073B5_A6Le_1_SV40pA3'element Reverse_H03_24_ABI24.ab1
    1 GATGGTAGGG GGGCGGAGCT ATGGAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
   61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
  121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
  181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
  241 TTGGCCGATT CATTAATGCA ACTATGGCCA TTTAATGTAA ATACTTAAGA AAAAAAACCA
  301 AATTAATTTT GATACATGCT GCATGTGAAA GACCCCCGCT GACGGGTAGT CAATCACTCA
  361 GAGGAGACCC TCCCAAGGAA CAGCGAGACC ACAAGTCGGA TGCAACTGCA AGAGGGTTTA
  421 TTGGATACAC GGGTACCCGG GCGACTCAGT CAATCGGAGG ACTGGCGCCC CGAGTGAGGG
  481 GTTGTGGGCT CTTTTATTGA GCTCGGGGAG CAGAAGCGCG CGAACAGAAG CGAGAAGCGA
  541 ACTGATTGGT TAGTTCAAAT AAGGCACAGG GTCATTTCAG GTCCTTGGGG CACCCTGGAA
  601 ACATCTGATG GTTCTCTAGA AACTGCTGAG GGCTGGACCG CATCTGGGGA CCATCTGTTC
  661 TTGGCCCTGA GCCGGGGCAG GAACTGCTTA CCACAGATAT CCTGTTTGGC CCATATTCAG
  721 CTGTTCCATC TGTTCTTGGC CCTGAGCCGG GGCAGGAACT GCTTACCACA GATATCCTGT
  781 TTGGCCCATA TTCAGCTGTT CCATCTGTTC CTGACCTTGA TCTGAACTTC TCTATTCTCA
  841 GTTATGTATT TTTTCCATGC CTTGCAAAAT GGCGTTACTT AAGCTAGCTT GCCAAACCTA
  901 CAGGTGGGGT CTTTCATTCC CCCCCTTTTT TCTGGAGACT AAATAAAATC TTTTATTTTA
  961 TCGTCGACCA CTGTGCTGGG CGGCCGGGCC GCTTTACTTG TACAGCTCGT CCATGCCCGA
 1021 GAGTGATCCC GGCGCGTCAC GACTCCAGCA GGAACAATGT GATCGCGCTT CTCGTTGGGT
1081 CTTTGCTCAA GTCGGACTGG GTGCCTCCAA GGTTAGGTTG GGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]