Resource data sheet
Prev.

pMXs-IRES-mCherry_IgE

Retroviral expression vector of mouse Immunoglobulin E (IgE).

Catalog number RDB14510
Resource name pMXs-IRES-mCherry_IgE
Clone info. Retroviral expression vector of mouse Immunoglobulin E (IgE), cloned into the pMXs-IRES-mCherry. Refer Fig.S1 of Haniuda, K., Nat. Immunol., 17 (9): 1109-1117 (2016).
Expression was confirmed by the depositor with flow cytometry. Sequence primer for IgH 5term-GCGGTCTATTATTGTGCAAG-3term. Known difference: The fluorescence isn't affected but there is CGG to TCT nucleotide substitution at nt684 to 686 (Gly229Leu) of the mCherry.
Vector backbone pMXs-IRES-mCherry (Plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name mouse Ighe cDNA
Depositor Kitamura, Daisuke |
 
Other clones in our bank mouse Ighe (NCBI Gene 380792) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions for distribution Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
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RDB14510 pMXs-IRES-mCherry_IgE DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14510_A6Lep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' RDB14510_A6Lea.seq
>D04073B1_A6Le_1_pBABE5'_F04_16_ABI24.ab1
    1 GTCAATATGG CATATGAGAT CTTATATGGG GCACCCCCGC CCCTTGTAAA CTTCCCTGAC
   61 CCTGACATGA CAAGAGTTAC TAACAGCCCC TCTCTCCAAG CTCACTTACA GGCTCTCTAC
  121 TTAGTCCAGC ACGAAGTCTG GAGACCTCTG GCGGCAGCCT ACCAAGAACA ACTGGACCGA
  181 CCGGTGGTAC CTCACCCTTA CCGAGTCGGC GACACAGTGT GGGTCCGCCG ACACCAGACT
  241 AAGAACCTAG AACCTCGCTG GAAAGGACCT TACACAGTCC TGCTGACCAC CCCCACCGCC
  301 CTCAAAGTAG ACGGCATCGC AGCTTGGATA CACGCCGCCC ACGTGAAGGC TGCCGACCCC
  361 GGGGGTGGAC CATCCTCTAG ACTGCCGGAT CCCAGTGTGG TGGTACGGGA ATTCACACAG
  421 GACCTCACCA TGGGATGGAG CTGTATCATG CTCTTCTTGG CAGCAACAGC TACAGGTGTC
  481 CACTCCCAGG TCCAACTGCA GCAGCCTGGG GCTGAGCTTG TGAAGCCTGG GGCTTCAGTG
  541 AAGCTGTCCT GCAAGGCTTC TGGCTACACC TTCACCAGCT ACTTGATGCA CTGGGTGAAG
  601 CAGAGGCCTG GACGAGGCCT TGAGTGGATT GGAAGGATTG ATCCTAATAG TGGTGGTACT
  661 AAGTACAATG AGAAGTTCAA GAGCAAGGCC ACACTGACTG TAGACAAACC CTCCAGCACA
  721 GCCTACATGC AGCTCAGCAG CCTGACATCT GAGGACTCTG CGGTCTATTA TTGTGCAAGA
  781 TATGATTACT ACGGTAGTAG CTACTTTGAC TACTGGGGCC AAGGCACCAC TCTCACAGTC
  841 TCGAGTTCTA TCAGGAACCC TCAGCTCTAC CCCTTGAAGC CCTGTAAAGG CACTGCTTCC
  901 ATGACCCTGG GCTGCCTGGT AAAGGGACTA CTTCCCTGGT CCTGTGACTG TGACCTGGTA
  961 TTCAGACTCC CTGAACATGA GCACTGTGAA CTTCCCTGCC CTTTGGGTTC TGACTCAAGT
 1021 CACCCACCCA AGCCCAAGTG ACCCAGCTGG GGGCAAGTCA GCCAAGGACT CACATGCCAC
1081 GTGGACACAT TCTTCAATCA ATTCCAACGA AGGTAAGGAA CTATTCCCTA GTTTCGGA
//
Primer: IRES-R RDB14510_A6Leb.seq
>D04073B1_A6Le_1_IRES-R_B03_06_ABI24.ab1
    1 GCGTCAGCGG CTTCGGCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGGGCGGA
   61 ATTTACGTAG CGGCCGCCTA TGCCCTGGTC TGGAAGATGT TGGCATAGTC TTGGAAGGTC
  121 TGGGGTGTAT CCTGCATCGG TGTGGAGAAA ACCCACTTCA CCTTGAGGAC GGTGACAGTG
  181 GCCCCATAGC TCACACTGAG CAGGAACAGG GTGATGAAGA CACAAATACT GGTCCACAGC
  241 TCTTCCAGCT CCTCGCCCTC CACCTCTTCA ATACATAGGT CCTGGAGGTC TAGCTCAAGG
  301 CTCGTGGATA TTGTTTTCTC CAGTTTCCTG GGTTTCTGAA GTGCCTCATG GATCACTTGG
  361 CAGGTGAACT GTTTTCTCTG TGTCCAGAGT GTCTTGGCGA CCTCTAGGCG ACTGAAGATG
  421 AAAAAGCCTT GATTGGAGCC ATTGGATTTC AGGGGTGTTG TGGTACTGTG ATGGCTGTTT
  481 GAGATCAGTT TGCCATCCTC CAGCCACTGC ACAGAGATAT CCTCAGGGAA GAAGTTCTGG
  541 ATCAAGCAGG TGAGTGTGCG TTTGTCCTCG CTCTCCTCCT CTGGTGGTGG GAACACATAT
  601 ACCTCGGGGG CTGAGCGCTG GCCTGGGGTC TTGGTGATGG AACGCACAAT GGGCTTGGGA
  661 AAATCAGGGT GGTCCACTAT GCACTGATAG CCGTAGCCTT CAATCCAGTC CTTGGCAACT
  721 ACAGGCAGGA TGGAGGTGAT ACTGGTTGTG GCGTTATGGT GGTGCTTAGT GTACCACTGG
  781 GATGCTGAGA CTGGAGTCTT CTTCTCTTGG TTCCACGTCA CATTGACATT CTTCTCGCTT
  841 TCCAGGTCCA CCACCAGACA GGTAAGCTTG GGAGCACCGT TTTGATACAG GTCCAGGGGG
  901 CTGGGTGGGA TCAGGTAGGT AATCACACCC CGTGGCTCAT GATCTGGGCA TCTCCGAGTG
  961 TGGGCCAAAT AGTCTACGCC TTGGGAGGTG ACCTTTGCAG TGAAGGTGCT TTCAGACATC
 1021 CATTGCTGCT CAGTGATGTG AGTTTACTGC AGTAGAGCTA GTTGCCTCCT CCTGATAGAC
 1081 AGTTGTGCAG TGTATCAGCT ATCTTCGAAT CGTCATAGCA GCCTGACGAA GACATCAATT
1141 TAAGGAATGG GTGGCTCAAT A
//
Primer: IRES-F RDB14510_A6Lec.seq
>D04073B1_A6Le_1_IRES-F_H04_22_ABI24.ab1
    1 GGCAAGTCTT ATGTGTTTAG TCGAGGTTAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA
   61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGGTG AGCAAGGGCG
  121 AGGAGGATAA CATGGCCATC ATCAAGGAGT TCATGCGCTT CAAGGTGCAC ATGGAGGGCT
  181 CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC TACGAGGGCA
  241 CCCAGACCGC CAAGCTGAAG GTGACCAAGG GTGGCCCCCT GCCCTTCGCC TGGGACATCC
  301 TGTCCCCTCA GTTCATGTAC GGCTCCAAGG CCTACGTGAA GCACCCCGCC GACATCCCCG
  361 ACTACTTGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG AACTTCGAGG
  421 ACGGCGGCGT GGTGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCGAG TTCATCTACA
  481 AGGTGAAGCT GCGCGGCACC AACTTCCCCT CCGACGGCCC CGTAATGCAG AAGAAGACCA
  541 TGGGCTGGGA GGCCTCCTCC GAGCGGATGT ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA
  601 TCAAGCAGAG GCTGAAGCTG AAGGACGGCG GCCACTACGA CGCTGAGGTC AAGACCACCT
  661 ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG GCGCCTACAA CGTCAACATC AAGTTGGACA
  721 TCACCTCCCA CAACGAGGAC TACACCATCG TGGAACAGTA CGAACGCGCC GAGGGCCGCC
  781 ACTCCACTCT CGGCATGGAC GAGCTGTACA AGTAAGCGGC CCGATAAAAT AAAAGATTTT
  841 ATTTAGTCTC CAGAAAAAGG GGGGAATGAA AGACCCCACC TGTAGTTTGG CAAGCTAGCT
  901 TAAGTAACGC CATTTTGCAA GGCATGGAAA AATACATAAC TGAGAATAGA GAAGTTCAGA
  961 TCAAGGGTCA GAACAGATGG GACAGCTGAA TATGGGCCCA AACAGGATAT CTGTGGTAAG
 1021 CAGTTTCCTG CCCCGGCTTC AGATCAAGAC AGATGGACAG CCTGGATATG GGTCAAACAG
 1081 ATATTCTGTG TTAGCCAGGT ACTTGCCGAC TCAGGTCAAG ACGATGACCC CAGATTGCCC
1141 GG
//
Primer: Amp_R RDB14510_A6Led.seq
>D04073B1_A6Le_1_Amp_R_F05_17_ABI24.ab1
    1 CTTTGTTTTC ATATTATTGA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
   61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
  121 ACCTGCATAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT
  181 TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAAAAGT TCAGATCAAG GTCAGGAACA
  241 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC
  301 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC
  361 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG
  421 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT
  481 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC
  541 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATTGACTGA
  601 GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT
  661 CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA
  721 TTTGGGGGCT CGTCCGGGAT CGGGAGACCC CTGCCCAGGG ACCACCGACC CACCACCGGG
  781 AGGTAAGCTG GCCAGCAACT TATCTGTGTC TGTCCGATTG TCTAGTGTCT ATGACTGATT
  841 TTATGCGCCT GCGTCGGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG
  901 GAACTGACGA GTTCGGAACA CCCGGCCGCA ACCCTGGGAG ACGTCCCAGG GACTTCGGGG
  961 GGCCGTTTTT GTGGCCCGAC CTGAGTCCCA AAAATCCCGA TCGTTTTGGG ACTCTTTGGG
 1021 TGCACCCCCC CTTTAGAAGG AAGGATATGT GGGTTCTGTT AAGAGACGAA CACCTAACAG
1081 ACCCGGCCTT CGGTCTGGAG TTTTTGCTTT CGGCTTTGGG GAACTGA
//
Primer: SV40pA3'element Reverse RDB14510_A6Lee.seq
>D04073B1_A6Le_1_SV40pA3element Reverse_G05_20_ABI24.ab1
    1 GAGTCAGGGG GGCGGACCTA TGGAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
   61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
  121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
  181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
  241 TTGGCCGATT CATTAATGCA ACTATGGCCA TTTAATGTAA ATACTTAAGA AAAAAAACCA
  301 AATTAATTTT GATACATGCT GCATGTGAAA GACCCCCGCT GACGGGTAGT CAATCACTCA
  361 GAGGAGACCC TCCCAAGGAA CAGCGAGACC ACAAGTCGGA TGCAACTGCA AGAGGGTTTA
  421 TTGGATACAC GGGTACCCGG GCGACTCAGT CAATCGGAGG ACTGGCGCCC CGAGTGAGGG
  481 GTTGTGGGCT CTTTTATTGA GCTCGGGGAG CAGAAGCGCG CGAACAGAAG CGAGAAGCGA
  541 ACTGATTGGT TAGTTCAAAT AAGGCACAGG GTCATTTCAG GTCCTTGGGG CACCCTGGAA
  601 ACATCTGATG GTTCTCTAGA AACTGCTGAG GGCTGGACCG CATCTGGGGA CCATCTGTTC
  661 TTGGCCCTGA GCCGGGGCAG GAACTGCTTA CCACAGATAT CCTGTTTGGC CCATATTCAG
  721 CTGTTCCATC TGTTCTTGGC CCTGAGCCGG GGCAGGAACT GCTTACCACA GATATCCTGT
  781 TTGGCCCATA TTCAGCTGTT CCATCTGTTC CTGACCTTGA TCTGAACTTC TCTATTCTCA
  841 GTTATGTATT TTTTCCATGC CTTGCAAAAT GGCGTTACTT AAGCTAGCTT GCCAAACCTA
  901 CAGGTGGGGG TCTTTCATTC CCCCCCTTTT TTCTGGAGAC TAAATAAAAT CTTTTTATTT
  961 TTATCGGGCC CGCTTTACTT TGTACAGCTC GTCCCATGCC CGAGAGGTGG AGTGGCGGGC
 1021 CCCTCCGGGC GGCGTTTCGT ACTGTTTCCC ACGATTGGTT GTTAGTTCCT TCGATTGTGG
1081 GAGTTGAATG ATCACTTGGA ATTGTTGAAC GATTGTTAAA CGTCGTCCCG T
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl