Resource data sheet
Prev.

pMXs-IRES-mCherry_IgG1

Retroviral expression vector of mouse Immunoglobulin G1 (IgG1).

Catalog number RDB14509
Resource name pMXs-IRES-mCherry_IgG1
Clone info. Retroviral expression vector of mouse Immunoglobulin G1 (IgG1), cloned into the pMXs-IRES-mCherry. Refer Fig.S1 of Haniuda, K., Nat. Immunol., 17 (9): 1109-1117 (2016).
Expression was confirmed by the depositor with flow cytometry. Sequence primer for IgH 5term-GCGGTCTATTATTGTGCAAG-3term. Known difference: The fluorescence isn't affected but there is CGG to TCT nucleotide substitution at nt684 to 686 (Gly229Leu) of the mCherry.
Vector backbone pMXs-IRES-mCherry (Plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name mouse Ighg1 cDNA
Depositor Kitamura, Daisuke |
 
Other clones in our bank mouse Ighg1 (NCBI Gene 16017) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions for distribution Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
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RDB14509 pMXs-IRES-mCherry_IgG1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14509_A6Lep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' RDB14509_A6Lea.seq
>D04073A9_A6Le_1_pBABE5'_E01_13_ABI24.ab1
    1 GTTTTATAAG GGCCATATGA GATCTTATAT GGGGCACCCC CGCCCCTTGT AAACTTCCCT
   61 GACCCTGACA TGACAAGAGT TACTAACAGC CCCTCTCTCC AAGCTCACTT ACAGGCTCTC
  121 TACTTAGTCC AGCACGAAGT CTGGAGACCT CTGGCGGCAG CCTACCAAGA ACAACTGGAC
  181 CGACCGGTGG TACCTCACCC TTACCGAGTC GGCGACACAG TGTGGGTCCG CCGACACCAG
  241 ACTAAGAACC TAGAACCTCG CTGGAAAGGA CCTTACACAG TCCTGCTGAC CACCCCCACC
  301 GCCCTCAAAG TAGACGGCAT CGCAGCTTGG ATACACGCCG CCCACGTGAA GGCTGCCGAC
  361 CCCGGGGGTG GACCATCCTC TAGACTGCCG GATCCCAGTG TGGTGGTACG GGAATTCACA
  421 CAGGACCTCA CCATGGGATG GAGCTGTATC ATGCTCTTCT TGGCAGCAAC AGCTACAGGT
  481 GTCCACTCCC AGGTCCAACT GCAGCAGCCT GGGGCTGAGC TTGTGAAGCC TGGGGCTTCA
  541 GTGAAGCTGT CCTGCAAGGC TTCTGGCTAC ACCTTCACCA GCTACTTGAT GCACTGGGTG
  601 AAGCAGAGGC CTGGACGAGG CCTTGAGTGG ATTGGAAGGA TTGATCCTAA TAGTGGTGGT
  661 ACTAAGTACA ATGAGAAGTT CAAGAGCAAG GCCACACTGA CTGTAGACAA ACCCTCCAGC
  721 ACAGCCTACA TGCAGCTCAG CAGCCTGACA TCTGAGGACT CTGCGGTCTA TTATTGTGCA
  781 AGATATGATT ACTACGGTAG TAGCTACTTT GACTACTGGG GCCAAGGCAC CACTCTCACA
  841 GTCTCGAGTG CCAAAACGAC ACCCCCATCT GTCTATCCAC TGGCCCCTGG ATCTGCTGCC
  901 CAAACTAACT CCATGGTGAC CCTGGGATGC CTGGTCAAGG GCTATTTCCC TGAGCCAGTG
  961 ACAGTGACCT GGAACTCTGG ATCCCTGTCC AGCGGTGTGC ACACCCTTCC CAGCTGTCCT
 1021 GCAGTCTGAC CTCTACACTC TGAGCAGCTC AGTGACTGTC CCCTCCAGCA CCTGGCCAAG
 1081 CAGGACGTCA CTGCACGTTG CCCAACCCGT CAGCAGCACA GTTGACAGAA TGTGCCAGGC
1141 ATGTGATGTA AGCCTATTGT GGCA
//
Primer: IRES-R RDB14509_A6Leb.seq
>D04073A9_A6Le_1_IRES-R_A03_03_ABI24.ab1
    1 GCATCAGCGG GGCTTCGGCA GTAACGTTAG GGGGGGGGGA GGGAGAGGGG GGGGGGGGGC
   61 GGAATTTACG TAGCGGCCGC CTAGGGCGCT TGCCCAATCA TGTTCTTGTA TTCAGGAACC
  121 AGTGTCTGCT TCAGCTCCAC CACCGAGGAG AAGATCCACT TTACCTTGAA GAGTGTGACA
  181 GCAGCGCTGT AGCACACACT GAGCAGGAAG AGGCTGATGA AGATGGTGAT GGTCGTCCAG
  241 AGCCCGTCCA GCTCCCCGTC CTGGGCCTCA GCACAGGTCT CGTCCAGTTG CAGCCCAGGA
  301 GAGTGGGAGA GGCTCTTCTC AGTATGGTGG TTGTGCAGGC CCTCATGTAA CACAGAGCAG
  361 GTGAAAGTAT TTCCTGCCTC CCAGTTGCTC TTCTGCACAT TGAGCTTGCT GTAGACGAAG
  421 TAAGAGCCAT CTGTGTCCAT GATGGGCTGA GTGTTCTTGT AGTTCTCCGC TGGCTGCCCA
  481 TTCCACTGCC ACTCCACAGT AATGTCTTCA GGGAAGAAGT TTGTTATCAT GCAGGTCAGA
  541 CTGACTTTAT CCTTGGCCAT CTGCTCCTTG GGAGGTGGAA TGGTGTACAC CTGTGGAGCC
  601 TTCGGTCTGC CTTTGGTTTT GGAGATGGTT TTCTCGATGG GGGCAGGGAA AGCTGCACTG
  661 TTGACCCTGC ATTTGAACTC CTTGCCATTG AGCCAGTCCT GGTGCATGAT GGGAAGTTCA
  721 CTGACTGAAC GGAAAGTGCT GTTGATCTGC TCCTCCCGGG GTTTCGTCTG AGCTGTGTGC
  781 ACCTCCACAT CATCTACAAA CCAGCTGAAC TGGACCTCGG GATCATCCTT GCTGATGTCT
  841 ACCACAACAC ACGTGACCTT AGGAGTCAGA GTAATGGTGA GCACATCCTT GGGCTTTGGG
  901 GGGAAGATGA AGACAGATGA TACTTCTGGG ACTGTACATA TGCAAGGCTT ACAACCACAA
  961 TCCCTGGGCA CAATTTTCTT GTCCACCTTT GGTGCTGCTG GCCGGGTGGG CAACGTTGCA
 1021 GTGACGGTCT GGCTGGGCCA GGTGCTGGAG GGGACAGTCA CTGAGCTGCT CAGAGTGTAG
 1081 AGTCAGACTG CAAGACAGCT GGCAGGTGTG CAACCGCTGG ACAGATTCCA GAGTTCAAGT
1141 CACTGTCACT GGCTTCAAGG
//
Primer: IRES-F RDB14509_A6Lec.seq
>D04073A9_A6Le_1_IRES-F_C12_09_ABI24.ab1
    1 TGCATCATTC TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC
   61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGGTGA GCAAGGGCGA
  121 GGAGGATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC AAGGTGCACA TGGAGGGCTC
  181 CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC
  241 CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG CCCTTCGCCT GGGACATCCT
  301 GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA
  361 CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA
  421 CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA
  481 GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT
  541 GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT
  601 CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGAGGTCA AGACCACCTA
  661 CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC GTCAACATCA AGTTGGACAT
  721 CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC GAACGCGCCG AGGGCCGCCA
  781 CTCCACTCTC GGCATGGACG AGCTGTACAA GTAAGCGGCC CGATAAAATA AAAGATTTTA
  841 TTTAGTCTCC AGAAAAAGGG GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT
  901 TAAGTAACGC CATTTTGCAA GGCATGGAAA AATACATAAC TGAGAATAGA GAAGTTTCAG
  961 ATCAAGGTCA GGAACAGATG GAAACAGCTG AATATGGGCC AAACAGATAT CTGTGGTAAG
 1021 CAGTTCCCTG CCCCGGGCTC AGGTCAGACA GATGGGACAG CTGATATGGG CCCAACAGGA
1081 TATCTGTGTT AGGCAGTCTG GCCTCCGGAC CTCCAGGTCC TAGA
//
Primer: Amp_R RDB14509_A6Led.seq
>D04073A9_A6Le_1_Amp_R_D12_12_ABI24.ab1
    1 CTTCGTTTTC ATATTATTGA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
   61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
  121 ACCTGCATAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT
  181 TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAAAAGT TCAGATCAAG GTCAGGAACA
  241 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC
  301 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC
  361 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG
  421 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT
  481 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC
  541 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATTGACTGA
  601 GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT
  661 CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA
  721 TTTGGGGGCT CGTCCGGGAT CGGGAGACCC CTGCCCAGGG ACCACCGACC CACCACCGGG
  781 AGGTAAGCTG GCCAGCAACT TATCTGTGTC TGTCCGATTG TCTAGTGTCT ATGACTGATT
  841 TTATGCGCCT GCGTCGGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG
  901 GAACTGACGA GTTCGGAACA CCCGGCCGCA ACCCTGGGAG ACGTCCCACG GACTTCGGGG
  961 GCCCGTTTTT TGTGGCCCGA CCTGAGTCCC AAAAATCCGA TCGTTTTTGG ACTCTTTGGG
 1021 TGCACCCCCC TTTAGAGGAG GGATATGTTG GATTCTGGGT AAGGAGACGA GACCTAAACC
1081 AGTTCCCCGC CTCGGTCTGA ATTTTTTGGC AATTCG
//
Primer: SV40pA3'element Reverse RDB14509_A6Lee.seq
>D04073A9_A6Le_1_SV40pA3element Reverse_E12_15_ABI24.ab1
    1 GTTGTCGGGG GGGCGGAGCC TATGGAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
   61 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA
  121 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA
  181 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC
  241 GTTGGCCGAT TCATTAATGC AACTATGGCC ATTTAATGTA AATACTTAAG AAAAAAAACC
  301 AAATTAATTT TGATACATGC TGCATGTGAA AGACCCCCGC TGACGGGTAG TCAATCACTC
  361 AGAGGAGACC CTCCCAAGGA ACAGCGAGAC CACAAGTCGG ATGCAACTGC AAGAGGGTTT
  421 ATTGGATACA CGGGTACCCG GGCGACTCAG TCAATCGGAG GACTGGCGCC CCGAGTGAGG
  481 GGTTGTGGGC TCTTTTATTG AGCTCGGGGA GCAGAAGCGC GCGAACAGAA GCGAGAAGCG
  541 AACTGATTGG TTAGTTCAAA TAAGGCACAG GGTCATTTCA GGTCCTTGGG GCACCCTGGA
  601 AACATCTGAT GGTTCTCTAG AAACTGCTGA GGGCTGGACC GCATCTGGGG ACCATCTGTT
  661 CTTGGCCCTG AGCCGGGGCA GGAACTGCTT ACCACAGATA TCCTGTTTGG CCCATATTCA
  721 GCTGTTCCAT CTGTTCTTGG CCCTGAGCCG GGGCAGGAAC TGCTTACCAC AGATATCCTG
  781 TTTGGCCCAT ATTCAGCTGT TCCATCTGTT CCTGACCTTG ATCTGAACTT CTCTATTCTC
  841 AGTTATGTAT TTTTTCCATG CCTTGCAAAA TGGCGTTACT TAAGCTAGCT TGCCAAACCT
  901 ACAGGTGGGG TCTTTCATTC CCCCCCTTTT TTCTGGAGAC TAAATAAAAT CTTTTTATTT
  961 TTATCGGGGC CGCTTTACTT GTAACAGCTT CGTTCCATGC CCGAGAGTGG AGTGGGCGGC
 1021 CCCTTCGGCG CGTTTCGTAC TGTTCCCACG GATGGTGTAG TCCCTCGATT GTGGGAGTGA
1081 ATGTTCTAAA CTTGAATGAT TGAACGGTTT GATAAGGGCT GTCCG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl