Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-IRES-GFP_IgE (#RDB14508)

Retroviral expression vector of mouse Immunoglobulin E (IgE).

Clone info. Retroviral expression vector of mouse Immunoglobulin E (IgE), cloned into the pMXs-IRES-GFP. Refer Fig.1 of Haniuda, K., Nat. Immunol., 17 (9): 1109-1117 (2016).
Comment Expression was confirmed by the depositor with flow cytometry. Sequence primer for IgH 5term-GCGGTCTATTATTGTGCAAG-3term.
Vector backbone pMXs-IRES-GFP (Plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name mouse Ighe cDNA
Depositor|Developer Kitamura, Daisuke |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions:
Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016） を引用し、寄託者への謝辞の表明を必要とする。
MTAに書く付加的使用条件：
pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14508 pMXs-IRES-GFP_IgE DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-IRES-GFP_IgE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14508).

Reference section:

Haniuda, K., Fukao, S., Kodama, T., Hasegawa, H., Kitamura, D., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14508_A6Lep1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5'
Sequence file: RDB14508_A6Lea.seq check
>D04073A8_A6Le_1_pBABE5'_A11_02_ABI24.ab1
    1 GCGAATATGG ACATATGAGA TCTTATATGG GGCACCCCCG CCCCTTGTAA ACTTCCCTGA
   61 CCCTGACATG ACAAGAGTTA CTAACAGCCC CTCTCTCCAA GCTCACTTAC AGGCTCTCTA
  121 CTTAGTCCAG CACGAAGTCT GGAGACCTCT GGCGGCAGCC TACCAAGAAC AACTGGACCG
  181 ACCGGTGGTA CCTCACCCTT ACCGAGTCGG CGACACAGTG TGGGTCCGCC GACACCAGAC
  241 TAAGAACCTA GAACCTCGCT GGAAAGGACC TTACACAGTC CTGCTGACCA CCCCCACCGC
  301 CCTCAAAGTA GACGGCATCG CAGCTTGGAT ACACGCCGCC CACGTGAAGG CTGCCGACCC
  361 CGGGGGTGGA CCATCCTCTA GACTGCCGGA TCCCAGTGTG GTGGTACGGG AATTCACACA
  421 GGACCTCACC ATGGGATGGA GCTGTATCAT GCTCTTCTTG GCAGCAACAG CTACAGGTGT
  481 CCACTCCCAG GTCCAACTGC AGCAGCCTGG GGCTGAGCTT GTGAAGCCTG GGGCTTCAGT
  541 GAAGCTGTCC TGCAAGGCTT CTGGCTACAC CTTCACCAGC TACTTGATGC ACTGGGTGAA
  601 GCAGAGGCCT GGACGAGGCC TTGAGTGGAT TGGAAGGATT GATCCTAATA GTGGTGGTAC
  661 TAAGTACAAT GAGAAGTTCA AGAGCAAGGC CACACTGACT GTAGACAAAC CCTCCAGCAC
  721 AGCCTACATG CAGCTCAGCA GCCTGACATC TGAGGACTCT GCGGTCTATT ATTGTGCAAG
  781 ATATGATTAC TACGGTAGTA GCTACTTTGA CTACTGGGGC CAAGGCACCA CTCTCACAGT
  841 CTCGAGTTCT ATCAGGAACC CTCAGCTCTA CCCCTTGAAG CCCTGTAAAG GCACTGCTTC
  901 CATGACCCTG GGCTGCCTGG TAAAGGACTA CTTCCCTGGT CCTGTGACTG TGACCTGGTA
  961 TTCAGACTCC CTGAACATGA GCACTGTGAA CTTCCCTGCC CTTGGATTCT GAACTCAAAG
 1021 TCACCACCCA GCCAAGTGAC CAGCTGGGGC AAGGTTCAGC TAGGACTTTC ACATGGCCAC
1081 GTGACACATC GTTCATTCAA TCCACGAAAG TAGGAACTTA ATCCTTAGAT CG
//
Primer: IRES-R
Sequence file: RDB14508_A6Leb.seq check
>D04073A8_A6Le_1_IRES-R_H02_23_ABI24.ab1
    1 GCATCAGCGG GCTTCGGCAG TAACGTTAGG GGGGGGGGAG GGAGAGGGGG GGGGGGGGCG
   61 GAATTTACGT AGCGGCCGCC TATGCCCTGG TCTGGAAGAT GTTGGCATAG TCTTGGAAGG
  121 TCTGGGGTGT ATCCTGCATC GGTGTGGAGA AGACCCACTT CACCTTGAGG ACGGTGACAG
  181 TGGCCCCATA GCTCACACTG AGCAGGAACA GGGTGATGAA GACACAAATA CTGGTCCACA
  241 GCTCTTCCAG CTCCTCGCCC TCCACCTCTT CAATACATAG GTCCTGGAGG TCTAGCTCAA
  301 GGCTCGTGGA TATTGTTTTC TCCAGTTTCC TGGGTTTCTG AAGTGCCTCA TGGATCACTT
  361 GGCAGGTGAA CTGTTTTCTC TGTGTCCAGA GTGTCTTGGC GACCTCTAGG CGACTGAAGA
  421 TGAAAAAGCC TTGATTGGAG CCATTGGATT TCAGGGGTGT TGTGGTACTG TGATGGCTGT
  481 TTGAGATCAG TTTGCCATCC TCCAGCCACT GCACAGAGAT ATCCTCAGGG AAGAAGTTCT
  541 GGATCAAGCA GGTGAGTGTG CGTTTGTCCT CGCTCTCCTC CTCTGGTGGT GGGAACACAT
  601 ATACCTCGGG GGCTGAGCGC TGGCCTGGGG TCTTGGTGAT GGAACGCACA ATGGGCTTGG
  661 GAAAATCAGG GTGGTCCACT ATGCACTGAT AGCCGTAGCC TTCAATCCAG TCCTTGGCAA
  721 CTACAGGCAG GATGGAGGTG ATACTGGTTG TGGCGTTATG GTGGTGCTTA GTGTACCACT
  781 GGGATGCTGA GACTGGAGTC TTCTTCTCTT GGTTCCACGT CACATTGACA TTCTTCTCGC
  841 TTTCCAGGTC CACCACCAGA CAGGTAAGCT TGGGAGCACC GTTTTGATAC AGGTCCAGGG
  901 GGCTGGGTGG GATCAGGTAG GTAATCACAC CCCGTGGCTC ATGATCTGGG CATCTCCGAG
  961 TGTGGGCCAA ATAGTCTACG CCTTGGGGAG GTGACCTTGC AGGTGAAGGT GCTTTCAGAC
 1021 ATCCATTGCT GCTCAGTGAT GTTGAGTTAC TGCAGTAGAG CTAGTTTGCT CCTCCTGAAT
 1081 AGACAGTTGT GCAGTGTATC AGTTATCTTC GATCGTCATT AAGCCTAGCT GACCGAAGAA
1141 CATCCAATTT AGGATGTGTG
//
Primer: IRES-F
Sequence file: RDB14508_A6Lec.seq check
>D04073A8_A6Le_1_IRES-F_C11_08_ABI24.ab1
    1 GCCATTCCTT TCTGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG
   61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC
  121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
  181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
  241 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
  301 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
  361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
  421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
  481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
  541 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC
  601 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG
  661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG
  721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
  781 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGGCCGCCA
  841 GCACAGTGGT CGACGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA GGGGGGGAAT
  901 GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTTAAGT AACGCCATTT TTGCAAGGGC
  961 ATGGAAAAAT ACATAACTGA GAATAGAGAA AGTTCAGATC CAAGGGTCAG AACAAGATGG
 1021 GAACAGCCTG AATATGGGCC AACAGGATAT CTGTGGGTAG GCAGTTCCTG CCCCGGCTCA
1081 AGCAGAACCA GATGAACAGC TGGAATATGG GTCCAAAACA AGGGAT
//
Primer: Amp_R
Sequence file: RDB14508_A6Led.seq check
>D04073A8_A6Le_1_Amp_R_D11_11_ABI24.ab1
    1 CTTTTTTTTA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT
  181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG
  241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
  301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA
  361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC
  421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG
  481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC
  541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATTGACTGAG
  601 TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT TGCATCCGAC TTGTGGTCTC
  661 GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG ATTGACTACC CGTCAGCGGG GGTCTTTCAT
  721 TTGGGGGCTC GTCCGGGATC GGGAGACCCC TGCCCAGGGA CCACCGACCC ACCACCGGGA
  781 GGTAAGCTGG CCAGCAACTT ATCTGTGTCT GTCCGATTGT CTAGTGTCTA TGACTGATTT
  841 TATGCGCCTG CGTCGGTACT AGTTAGCTAA CTAGCTCTGT ATCTGGCGGA CCCGTGGTGG
  901 AACTGACGAG TTCGGAACAC CCGGCCGCAA CCCTGGGAGA CGTCCCAGGG ACTTCGGGGG
  961 GGCCGTTTTT GTGGGCCCGA CCTGAGTCCC AAAAAATCCC GATCGTTTTG GACTCTTTTG
 1021 GTTGCACCCC CCCTTAGAGG AGGATATGTT GGTTCCTGTT AAGAGACGAG AACTAAACAG
1081 TCCGGCCTTC GTCTGGATTT TTGGCCTTTC GGTTTTTGGG GAACCCGA
//
Primer: SV40pA3'element Reverse
Sequence file: RDB14508_A6Lee.seq check
>D04073A8_A6Le_1_SV40pA3element Reverse_E11_14_ABI24.ab1
    1 GTAGGTTTGG GGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT
   61 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG
  121 TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG
  181 AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC
  241 CCGCGCGTTG GCCGATTCAT TAATGCAACT ATGGCCATTT AATGTAAATA CTTAAGAAAA
  301 AAAACCAAAT TAATTTTGAT ACATGCTGCA TGTGAAAGAC CCCCGCTGAC GGGTAGTCAA
  361 TCACTCAGAG GAGACCCTCC CAAGGAACAG CGAGACCACA AGTCGGATGC AACTGCAAGA
  421 GGGTTTATTG GATACACGGG TACCCGGGCG ACTCAGTCAA TCGGAGGACT GGCGCCCCGA
  481 GTGAGGGGTT GTGGGCTCTT TTATTGAGCT CGGGGAGCAG AAGCGCGCGA ACAGAAGCGA
  541 GAAGCGAACT GATTGGTTAG TTCAAATAAG GCACAGGGTC ATTTCAGGTC CTTGGGGCAC
  601 CCTGGAAACA TCTGATGGTT CTCTAGAAAC TGCTGAGGGC TGGACCGCAT CTGGGGACCA
  661 TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA CTGCTTACCA CAGATATCCT GTTTGGCCCA
  721 TATTCAGCTG TTCCATCTGT TCTTGGCCCT GAGCCGGGGG CAGGAACTGC TTACCACAGA
  781 TATCCTGTTT GGCCCATATT CAGCTGTTCC ATCTGTTCCT GACCTTGATC TGAACTTCTC
  841 AACCCCCCCT CCCACTCCAC ATAGAGATTT TTTTCCCCCC CCCGCGAAAA AAGGGGGGTT
  901 ATATATATAC AAAGCTGCCA GGCGGGGGGT CGTTTCGGTG TCCCCCCCTT TTTTTCCTGG
  961 GAGAACTAAA TAAAAATCCT TTTTATTTTT TATCCGTTCG ACCAACTGTT GCCTGGGCGG
 1021 GCCGGGCCCG CCTTTTACCT TTGTACCAGC CTTCGGTTCC CAATGGCCCG AGAAGGTGGA
 1081 ATCCCCCAGG CCGGGCGGGG TCCACCGGAA CTTCCAATCA GGAACCCCAT GGGTGAATCC
1141 GCCGGCTTTC TCTCCGTTTG GGGGGGACCT TTGTGCCCTC TCACGGGGGC CTCGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]