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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-IRES-GFP_IgG1 (#RDB14504)

Retroviral expression vector of mouse Immunoglobulin G1 (IgG1).

Clone info. Retroviral expression vector of mouse Immunoglobulin G1 (IgG1), cloned into the pMXs-IRES-GFP. Refer Fig.1 of Haniuda, K., Nat. Immunol., 17 (9): 1109-1117 (2016).
Comment Expression was confirmed by the depositor with flow cytometry. Sequence primer for IgH 5term-GCGGTCTATTATTGTGCAAG-3term.
Vector backbone pMXs-IRES-GFP (Plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name mouse Ighg1 cDNA
Depositor|Developer Kitamura, Daisuke |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions:
Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016） を引用し、寄託者への謝辞の表明を必要とする。
MTAに書く付加的使用条件：
pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14504 pMXs-IRES-GFP_IgG1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-IRES-GFP_IgG1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14504).

Reference section:

Haniuda, K., Fukao, S., Kodama, T., Hasegawa, H., Kitamura, D., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14504_A6Lep1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5'
Sequence file: RDB14504_A6Lea.seq check
>D04073A4_A6Le_1_pBABE5'_B01_04_ABI24.ab1
    1 GTCTATATGG TCATATGAGA TCTTATATGG GGCACCCCCG CCCCTTGTAA ACTTCCCTGA
   61 CCCTGACATG ACAAGAGTTA CTAACAGCCC CTCTCTCCAA GCTCACTTAC AGGCTCTCTA
  121 CTTAGTCCAG CACGAAGTCT GGAGACCTCT GGCGGCAGCC TACCAAGAAC AACTGGACCG
  181 ACCGGTGGTA CCTCACCCTT ACCGAGTCGG CGACACAGTG TGGGTCCGCC GACACCAGAC
  241 TAAGAACCTA GAACCTCGCT GGAAAGGACC TTACACAGTC CTGCTGACCA CCCCCACCGC
  301 CCTCAAAGTA GACGGCATCG CAGCTTGGAT ACACGCCGCC CACGTGAAGG CTGCCGACCC
  361 CGGGGGTGGA CCATCCTCTA GACTGCCGGA TCCCAGTGTG GTGGTACGGG AATTCACACA
  421 GGACCTCACC ATGGGATGGA GCTGTATCAT GCTCTTCTTG GCAGCAACAG CTACAGGTGT
  481 CCACTCCCAG GTCCAACTGC AGCAGCCTGG GGCTGAGCTT GTGAAGCCTG GGGCTTCAGT
  541 GAAGCTGTCC TGCAAGGCTT CTGGCTACAC CTTCACCAGC TACTTGATGC ACTGGGTGAA
  601 GCAGAGGCCT GGACGAGGCC TTGAGTGGAT TGGAAGGATT GATCCTAATA GTGGTGGTAC
  661 TAAGTACAAT GAGAAGTTCA AGAGCAAGGC CACACTGACT GTAGACAAAC CCTCCAGCAC
  721 AGCCTACATG CAGCTCAGCA GCCTGACATC TGAGGACTCT GCGGTCTATT ATTGTGCAAG
  781 ATATGATTAC TACGGTAGTA GCTACTTTGA CTACTGGGGC CAAGGCACCA CTCTCACAGT
  841 CTCGAGTGCC AAAACGACAC CCCCATCTGT CTATCCACTG GCCCCTGGAT CTGCTGCCCA
  901 AACTAACTCC ATGGTGACCC TGGGATGCCT GGTCAAGGGC TATTTCCCTG AGCCAGTGAC
  961 AGTGACCTGG AACTCTGGAT CCCTGTCCAG CGGTGTGCAC ACCTTCCCAG CTGTCCTGCA
 1021 GTCTGACCTC TACACTCTGA GCAGCTCAGT GACTGTCCCC TCCAGCACCT GGGCCCCAGC
 1081 CCAGACCGTT CATCTTGCTA CGATGCTCCC ACCCGATAGC CAGCCACAGG TGGGACAACA
1141 ATGTGCTCAG GATGTGTGTA AGACCTTGGC ACATATAATG TGGGTAC
//
Primer: IRES-R
Sequence file: RDB14504_A6Leb.seq check
>D04073A4_A6Le_1_IRES-R_D02_11_ABI24.ab1
    1 GCATCAGCGG GCTTCGGCAG TAACGTTAGG GGGGGGGGAG GGGAGAGGGG GGGGGGGGGC
   61 GGAATTTACG TAGCGGCCGC CTAGGGCGCT TGCCCAATCA TGTTCTTGTA TTCAGGAACC
  121 AGTGTCTGCT TCAGCTCCAC CACCGAGGAG AAGATCCACT TTACCTTGAA GAGTGTGACA
  181 GCAGCGCTGT AGCACACACT GAGCAGGAAG AGGCTGATGA AGATGGTGAT GGTCGTCCAG
  241 AGCCCGTCCA GCTCCCCGTC CTGGGCCTCA GCACAGGTCT CGTCCAGTTG CAGCCCAGGA
  301 GAGTGGGAGA GGCTCTTCTC AGTATGGTGG TTGTGCAGGC CCTCATGTAA CACAGAGCAG
  361 GTGAAAGTAT TTCCTGCCTC CCAGTTGCTC TTCTGCACAT TGAGCTTGCT GTAGACGAAG
  421 TAAGAGCCAT CTGTGTCCAT GATGGGCTGA GTGTTCTTGT AGTTCTCCGC TGGCTGCCCA
  481 TTCCACTGCC ACTCCACAGT AATGTCTTCA GGGAAGAAGT TTGTTATCAT GCAGGTCAGA
  541 CTGACTTTAT CCTTGGCCAT CTGCTCCTTG GGAGGTGGAA TGGTGTACAC CTGTGGAGCC
  601 TTCGGTCTGC CTTTGGTTTT GGAGATGGTT TTCTCGATGG GGGCAGGGAA AGCTGCACTG
  661 TTGACCCTGC ATTTGAACTC CTTGCCATTG AGCCAGTCCT GGTGCATGAT GGGAAGTTCA
  721 CTGACTGAAC GGAAAGTGCT GTTGATCTGC TCCTCCCGGG GTTTCGTCTG AGCTGTGTGC
  781 ACCTCCACAT CATCTACAAA CCAGCTGAAC TGGACCTCGG GATCATCCTT GCTGATGTCT
  841 ACCACAACAC ACGTGACCTT AGGAGTCAGA GTAATGGTGA GCACATCCTT GGGCTTTGGG
  901 GGGAAGATGA AGACAGATGA TACTTCTGGG ACTGTACATA TGCAAGGCTT ACAACCACAA
  961 TCCCTGGGCA CAATTTTCTT GTCCACCTTG GTGCTGCTGG CCGGGTGGCA ACGTTGCAGT
 1021 GACGGTCTGG CTGGCCCAGG TGCTGGAGGG GACAGTCACT GAGCTGCTCA GAGTGTAGAG
 1081 GTCAGACTGC AGACAGCTGC AGTGTGCACA CCGCTGGACA GGCAATCCAG AGCTCCAGCT
1141 CACACTGTTT CACACTTG
//
Primer: IRES-F
Sequence file: RDB14504_A6Lec.seq check
>D04073A4_A6Le_1_IRES-F_C07_07_ABI24.ab1
    1 GGCCGGGCTT TCCTGTGTTT AGTCGAGGTT AAAAACGTCT AGGCCCCCCG AACCACGGGG
   61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC
  121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
  181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
  241 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
  301 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
  361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
  421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
  481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
  541 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC
  601 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG
  661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG
  721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
  781 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGGCCGCCA
  841 GCACAGTGGT CGACGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA GGGGGGAATG
  901 AAAGACCCCA CCTGTAGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC AAAGGCATGG
  961 GAAAAAATAC ATAACTGAGA ATAGGAGAAA GTTTCAGATC AAGGTCAGAA CAGATGGGAA
 1021 ACAGCTGATA ATGGGGCCCA AAACAGATAA TCTGTTGGTT AGGCAGTTTC CTGCCCCGGC
1081 TTCAAGTCAA GAACCAGGAT GGAAACAGCT GGCAATATGG CCCCAAAAAC AGA
//
Primer: Amp_R
Sequence file: RDB14504_A6Led.seq check
>D04073A4_A6Le_1_Amp_R_D07_10_ABI24.ab1
    1 TTTCCTTTTT TATATTATTG AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
   61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
  121 CACCTGCATA ATGAAAGACC CCACCTGTAG GTTTGGCAAG CTAGCTTAAG TAACGCCATT
  181 TTGCAAGGCA TGGAAAAATA CATAACTGAG AATAGAAAAG TTCAGATCAA GGTCAGGAAC
  241 AGATGGAACA GCTGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG
  301 CTCAGGGCCA AGAACAGATG GAACAGCTGA ATATGGGCCA AACAGGATAT CTGTGGTAAG
  361 CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC AGATGGTCCC CAGATGCGGT CCAGCCCTCA
  421 GCAGTTTCTA GAGAACCATC AGATGTTTCC AGGGTGCCCC AAGGACCTGA AATGACCCTG
  481 TGCCTTATTT GAACTAACCA ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC
  541 CCGAGCTCAA TAAAAGAGCC CACAACCCCT CACTCGGCGC GCCAGTCCTC CGATTGACTG
  601 AGTCGCCCGG GTACCCGTGT ATCCAATAAA CCCTCTTGCA GTTGCATCCG ACTTGTGGTC
  661 TCGCTGTTCC TTGGGAGGGT CTCCTCTGAG TGATTGACTA CCCGTCAGCG GGGGTCTTTC
  721 ATTTGGGGGC TCGTCCGGGA TCGGGAGACC CCTGCCCAGG GACCACCGAC CCACCACCGG
  781 GAGGTAAGCT GGCCAGCAAC TTATCTGTGT CTGTCCGATT GTCTAGTGTC TATGACTGAT
  841 TTTATGCGCC TGCGTCGGTA CTAGTTAGCT AACTAGCTCT GTATCTGGCG GACCCGTGGT
  901 GGAACTGACG AGTTCGGAAC ACCCGGCCGC AACCCTGGGA GACGTCCCAG GGACTTCGGG
  961 GGGCCGTTTT TTGTGGCCCG ACCTGAGTCC AAAAATCCCG ATCGTTTTGG ACTCTTTGGG
 1021 TGCACCCCCC CCTTTAGAAG AGGGATATGT GGGATTCTGG TAGGAGACGA AGAACTAAAC
1081 CAGTCGGCCT TCCGGTCTGC AATTTTTGCC TTTCGGATTG GGGAAACCCG AGC
//
Primer: SV40pA3'element Reverse
Sequence file: RDB14504_A6Lee.seq check
>D04073A4_A6Le_1_SV40pA3element Reverse_E07_13_ABI24.ab1
    1 GACTCGGGGG GGGCGGAGCC TATGGAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
   61 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA
  121 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA
  181 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC
  241 GTTGGCCGAT TCATTAATGC AACTATGGCC ATTTAATGTA AATACTTAAG AAAAAAAACC
  301 AAATTAATTT TGATACATGC TGCATGTGAA AGACCCCCGC TGACGGGTAG TCAATCACTC
  361 AGAGGAGACC CTCCCAAGGA ACAGCGAGAC CACAAGTCGG ATGCAACTGC AAGAGGGTTT
  421 ATTGGATACA CGGGTACCCG GGCGACTCAG TCAATCGGAG GACTGGCGCC CCGAGTGAGG
  481 GGTTGTGGGC TCTTTTATTG AGCTCGGGGA GCAGAAGCGC GCGAACAGAA GCGAGAAGCG
  541 AACTGATTGG TTAGTTCAAA TAAGGCACAG GGTCATTTCA GGTCCTTGGG GCACCCTGGA
  601 AACATCTGAT GGTTCTCTAG AAACTGCTGA GGGCTGGACC GCATCTGGGG ACCATCTGTT
  661 CTTGGCCCTG AGCCGGGGCA GGAACTGCTT ACCACAGATA TCCTGTTTGG CCCATATTCA
  721 GCTGTTCCAT CTGTTCTTGG CCCTGAGCCG GGGCAGGAAC TGCTTACCAC AGATATCCTG
  781 TTTGGCCCAT ATTCAGCTGT TCCATCTGTT CCTGACCTTG ATCTGAACTT CTCTATTCTC
  841 AGTTATGTAT TTTTTCCATG CCTTGCAAAA TGGCGTTACT TAAGCTAGCT TGCCAAACCT
  901 ACAGGTGGGG TCTTTCATTC CCCCCCTTTT TTCTGGAGAC TAAATAAAAA TCTTTTTATT
  961 TTATCGTCGA CCCACTGTGC TGGGCGGGCC GGGCCCGCTT TTACTTTGTA CAGCTCGTCC
 1021 ATGCCGAGAG TGATCCCAGC GGCGGGTCAC GACTTCCAAG CACGACCATG TTGAATCGCG
1081 GCTTCTCGTT GGATCTTTGC TTCACGCTCG ACTGGAATTG ACTCAAGGGG ATA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]