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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-IRES-GFP_IgG1

Retroviral expression vector of mouse Immunoglobulin G1 (IgG1).

Catalog number RDB14504
Resource name pMXs-IRES-GFP_IgG1
Clone info. Retroviral expression vector of mouse Immunoglobulin G1 (IgG1), cloned into the pMXs-IRES-GFP. Refer Fig.1 of Haniuda, K., Nat. Immunol., 17 (9): 1109-1117 (2016).
Comment Expression was confirmed by the depositor with flow cytometry. Sequence primer for IgH 5term-GCGGTCTATTATTGTGCAAG-3term.
Vector backbone pMXs-IRES-GFP (Plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name mouse Ighg1 cDNA
Depositor|Developer Kitamura, Daisuke |
Other clones in our bank

External Database
mouse Ighg1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
          Additional terms and conditions:
          Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) を引用し、寄託者への謝辞の表明を必要とする。
          付加的提供条件:
          pMXsベクターについて:利用者は、研究成果の公表にあたって pMXsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄先生への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14504 pMXs-IRES-GFP_IgG1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14504_A6Lep1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pBABE5'
          Sequence file: RDB14504_A6Lea.seq check
          >D04073A4_A6Le_1_pBABE5'_B01_04_ABI24.ab1
              1 GTCTATATGG TCATATGAGA TCTTATATGG GGCACCCCCG CCCCTTGTAA ACTTCCCTGA
             61 CCCTGACATG ACAAGAGTTA CTAACAGCCC CTCTCTCCAA GCTCACTTAC AGGCTCTCTA
            121 CTTAGTCCAG CACGAAGTCT GGAGACCTCT GGCGGCAGCC TACCAAGAAC AACTGGACCG
            181 ACCGGTGGTA CCTCACCCTT ACCGAGTCGG CGACACAGTG TGGGTCCGCC GACACCAGAC
            241 TAAGAACCTA GAACCTCGCT GGAAAGGACC TTACACAGTC CTGCTGACCA CCCCCACCGC
            301 CCTCAAAGTA GACGGCATCG CAGCTTGGAT ACACGCCGCC CACGTGAAGG CTGCCGACCC
            361 CGGGGGTGGA CCATCCTCTA GACTGCCGGA TCCCAGTGTG GTGGTACGGG AATTCACACA
            421 GGACCTCACC ATGGGATGGA GCTGTATCAT GCTCTTCTTG GCAGCAACAG CTACAGGTGT
            481 CCACTCCCAG GTCCAACTGC AGCAGCCTGG GGCTGAGCTT GTGAAGCCTG GGGCTTCAGT
            541 GAAGCTGTCC TGCAAGGCTT CTGGCTACAC CTTCACCAGC TACTTGATGC ACTGGGTGAA
            601 GCAGAGGCCT GGACGAGGCC TTGAGTGGAT TGGAAGGATT GATCCTAATA GTGGTGGTAC
            661 TAAGTACAAT GAGAAGTTCA AGAGCAAGGC CACACTGACT GTAGACAAAC CCTCCAGCAC
            721 AGCCTACATG CAGCTCAGCA GCCTGACATC TGAGGACTCT GCGGTCTATT ATTGTGCAAG
            781 ATATGATTAC TACGGTAGTA GCTACTTTGA CTACTGGGGC CAAGGCACCA CTCTCACAGT
            841 CTCGAGTGCC AAAACGACAC CCCCATCTGT CTATCCACTG GCCCCTGGAT CTGCTGCCCA
            901 AACTAACTCC ATGGTGACCC TGGGATGCCT GGTCAAGGGC TATTTCCCTG AGCCAGTGAC
            961 AGTGACCTGG AACTCTGGAT CCCTGTCCAG CGGTGTGCAC ACCTTCCCAG CTGTCCTGCA
           1021 GTCTGACCTC TACACTCTGA GCAGCTCAGT GACTGTCCCC TCCAGCACCT GGGCCCCAGC
           1081 CCAGACCGTT CATCTTGCTA CGATGCTCCC ACCCGATAGC CAGCCACAGG TGGGACAACA
          1141 ATGTGCTCAG GATGTGTGTA AGACCTTGGC ACATATAATG TGGGTAC
          //
          Primer: IRES-R
          Sequence file: RDB14504_A6Leb.seq check
          >D04073A4_A6Le_1_IRES-R_D02_11_ABI24.ab1
              1 GCATCAGCGG GCTTCGGCAG TAACGTTAGG GGGGGGGGAG GGGAGAGGGG GGGGGGGGGC
             61 GGAATTTACG TAGCGGCCGC CTAGGGCGCT TGCCCAATCA TGTTCTTGTA TTCAGGAACC
            121 AGTGTCTGCT TCAGCTCCAC CACCGAGGAG AAGATCCACT TTACCTTGAA GAGTGTGACA
            181 GCAGCGCTGT AGCACACACT GAGCAGGAAG AGGCTGATGA AGATGGTGAT GGTCGTCCAG
            241 AGCCCGTCCA GCTCCCCGTC CTGGGCCTCA GCACAGGTCT CGTCCAGTTG CAGCCCAGGA
            301 GAGTGGGAGA GGCTCTTCTC AGTATGGTGG TTGTGCAGGC CCTCATGTAA CACAGAGCAG
            361 GTGAAAGTAT TTCCTGCCTC CCAGTTGCTC TTCTGCACAT TGAGCTTGCT GTAGACGAAG
            421 TAAGAGCCAT CTGTGTCCAT GATGGGCTGA GTGTTCTTGT AGTTCTCCGC TGGCTGCCCA
            481 TTCCACTGCC ACTCCACAGT AATGTCTTCA GGGAAGAAGT TTGTTATCAT GCAGGTCAGA
            541 CTGACTTTAT CCTTGGCCAT CTGCTCCTTG GGAGGTGGAA TGGTGTACAC CTGTGGAGCC
            601 TTCGGTCTGC CTTTGGTTTT GGAGATGGTT TTCTCGATGG GGGCAGGGAA AGCTGCACTG
            661 TTGACCCTGC ATTTGAACTC CTTGCCATTG AGCCAGTCCT GGTGCATGAT GGGAAGTTCA
            721 CTGACTGAAC GGAAAGTGCT GTTGATCTGC TCCTCCCGGG GTTTCGTCTG AGCTGTGTGC
            781 ACCTCCACAT CATCTACAAA CCAGCTGAAC TGGACCTCGG GATCATCCTT GCTGATGTCT
            841 ACCACAACAC ACGTGACCTT AGGAGTCAGA GTAATGGTGA GCACATCCTT GGGCTTTGGG
            901 GGGAAGATGA AGACAGATGA TACTTCTGGG ACTGTACATA TGCAAGGCTT ACAACCACAA
            961 TCCCTGGGCA CAATTTTCTT GTCCACCTTG GTGCTGCTGG CCGGGTGGCA ACGTTGCAGT
           1021 GACGGTCTGG CTGGCCCAGG TGCTGGAGGG GACAGTCACT GAGCTGCTCA GAGTGTAGAG
           1081 GTCAGACTGC AGACAGCTGC AGTGTGCACA CCGCTGGACA GGCAATCCAG AGCTCCAGCT
          1141 CACACTGTTT CACACTTG
          //
          Primer: IRES-F
          Sequence file: RDB14504_A6Lec.seq check
          >D04073A4_A6Le_1_IRES-F_C07_07_ABI24.ab1
              1 GGCCGGGCTT TCCTGTGTTT AGTCGAGGTT AAAAACGTCT AGGCCCCCCG AACCACGGGG
             61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC
            121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
            181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
            241 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
            301 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
            361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
            421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
            481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
            541 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC
            601 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG
            661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG
            721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
            781 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGGCCGCCA
            841 GCACAGTGGT CGACGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA GGGGGGAATG
            901 AAAGACCCCA CCTGTAGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC AAAGGCATGG
            961 GAAAAAATAC ATAACTGAGA ATAGGAGAAA GTTTCAGATC AAGGTCAGAA CAGATGGGAA
           1021 ACAGCTGATA ATGGGGCCCA AAACAGATAA TCTGTTGGTT AGGCAGTTTC CTGCCCCGGC
          1081 TTCAAGTCAA GAACCAGGAT GGAAACAGCT GGCAATATGG CCCCAAAAAC AGA
          //
          Primer: Amp_R
          Sequence file: RDB14504_A6Led.seq check
          >D04073A4_A6Le_1_Amp_R_D07_10_ABI24.ab1
              1 TTTCCTTTTT TATATTATTG AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
             61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
            121 CACCTGCATA ATGAAAGACC CCACCTGTAG GTTTGGCAAG CTAGCTTAAG TAACGCCATT
            181 TTGCAAGGCA TGGAAAAATA CATAACTGAG AATAGAAAAG TTCAGATCAA GGTCAGGAAC
            241 AGATGGAACA GCTGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG
            301 CTCAGGGCCA AGAACAGATG GAACAGCTGA ATATGGGCCA AACAGGATAT CTGTGGTAAG
            361 CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC AGATGGTCCC CAGATGCGGT CCAGCCCTCA
            421 GCAGTTTCTA GAGAACCATC AGATGTTTCC AGGGTGCCCC AAGGACCTGA AATGACCCTG
            481 TGCCTTATTT GAACTAACCA ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC
            541 CCGAGCTCAA TAAAAGAGCC CACAACCCCT CACTCGGCGC GCCAGTCCTC CGATTGACTG
            601 AGTCGCCCGG GTACCCGTGT ATCCAATAAA CCCTCTTGCA GTTGCATCCG ACTTGTGGTC
            661 TCGCTGTTCC TTGGGAGGGT CTCCTCTGAG TGATTGACTA CCCGTCAGCG GGGGTCTTTC
            721 ATTTGGGGGC TCGTCCGGGA TCGGGAGACC CCTGCCCAGG GACCACCGAC CCACCACCGG
            781 GAGGTAAGCT GGCCAGCAAC TTATCTGTGT CTGTCCGATT GTCTAGTGTC TATGACTGAT
            841 TTTATGCGCC TGCGTCGGTA CTAGTTAGCT AACTAGCTCT GTATCTGGCG GACCCGTGGT
            901 GGAACTGACG AGTTCGGAAC ACCCGGCCGC AACCCTGGGA GACGTCCCAG GGACTTCGGG
            961 GGGCCGTTTT TTGTGGCCCG ACCTGAGTCC AAAAATCCCG ATCGTTTTGG ACTCTTTGGG
           1021 TGCACCCCCC CCTTTAGAAG AGGGATATGT GGGATTCTGG TAGGAGACGA AGAACTAAAC
          1081 CAGTCGGCCT TCCGGTCTGC AATTTTTGCC TTTCGGATTG GGGAAACCCG AGC
          //
          Primer: SV40pA3'element Reverse
          Sequence file: RDB14504_A6Lee.seq check
          >D04073A4_A6Le_1_SV40pA3element Reverse_E07_13_ABI24.ab1
              1 GACTCGGGGG GGGCGGAGCC TATGGAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
             61 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA
            121 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA
            181 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC
            241 GTTGGCCGAT TCATTAATGC AACTATGGCC ATTTAATGTA AATACTTAAG AAAAAAAACC
            301 AAATTAATTT TGATACATGC TGCATGTGAA AGACCCCCGC TGACGGGTAG TCAATCACTC
            361 AGAGGAGACC CTCCCAAGGA ACAGCGAGAC CACAAGTCGG ATGCAACTGC AAGAGGGTTT
            421 ATTGGATACA CGGGTACCCG GGCGACTCAG TCAATCGGAG GACTGGCGCC CCGAGTGAGG
            481 GGTTGTGGGC TCTTTTATTG AGCTCGGGGA GCAGAAGCGC GCGAACAGAA GCGAGAAGCG
            541 AACTGATTGG TTAGTTCAAA TAAGGCACAG GGTCATTTCA GGTCCTTGGG GCACCCTGGA
            601 AACATCTGAT GGTTCTCTAG AAACTGCTGA GGGCTGGACC GCATCTGGGG ACCATCTGTT
            661 CTTGGCCCTG AGCCGGGGCA GGAACTGCTT ACCACAGATA TCCTGTTTGG CCCATATTCA
            721 GCTGTTCCAT CTGTTCTTGG CCCTGAGCCG GGGCAGGAAC TGCTTACCAC AGATATCCTG
            781 TTTGGCCCAT ATTCAGCTGT TCCATCTGTT CCTGACCTTG ATCTGAACTT CTCTATTCTC
            841 AGTTATGTAT TTTTTCCATG CCTTGCAAAA TGGCGTTACT TAAGCTAGCT TGCCAAACCT
            901 ACAGGTGGGG TCTTTCATTC CCCCCCTTTT TTCTGGAGAC TAAATAAAAA TCTTTTTATT
            961 TTATCGTCGA CCCACTGTGC TGGGCGGGCC GGGCCCGCTT TTACTTTGTA CAGCTCGTCC
           1021 ATGCCGAGAG TGATCCCAGC GGCGGGTCAC GACTTCCAAG CACGACCATG TTGAATCGCG
          1081 GCTTCTCGTT GGATCTTTGC TTCACGCTCG ACTGGAATTG ACTCAAGGGG ATA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827.

          2023.04.10

          GNP_filter3_RDBDEP_html_230405.pl