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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-IRES-GFP_IgG3 (#RDB14503)

Retroviral expression vector of mouse Immunoglobulin G3 (IgG3).

Clone info. Retroviral expression vector of mouse Immunoglobulin G3 (IgG3), cloned into the pMXs-IRES-GFP. Refer Fig.1 of Haniuda, K., Nat. Immunol., 17 (9): 1109-1117 (2016).
Comment Expression was confirmed by the depositor with flow cytometry. Sequence primer for IgH 5term-GCGGTCTATTATTGTGCAAG-3term.
Vector backbone pMXs-IRES-GFP (Plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name mouse Ighg3 cDNA
Depositor|Developer Kitamura, Daisuke |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions:
Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016） を引用し、寄託者への謝辞の表明を必要とする。
MTAに書く付加的使用条件：
pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14503 pMXs-IRES-GFP_IgG3 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-IRES-GFP_IgG3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14503).

Reference section:

Haniuda, K., Fukao, S., Kodama, T., Hasegawa, H., Kitamura, D., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14503_A6Lep1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5'
Sequence file: RDB14503_A6Lea.seq check
>D04073A3_A6Le_1_pBABE5'_A06_03_ABI24.ab1
    1 GCGATATGGA CATATGAGAT CTTATATGGG GCACCCCCGC CCCTTGTAAA CTTCCCTGAC
   61 CCTGACATGA CAAGAGTTAC TAACAGCCCC TCTCTCCAAG CTCACTTACA GGCTCTCTAC
  121 TTAGTCCAGC ACGAAGTCTG GAGACCTCTG GCGGCAGCCT ACCAAGAACA ACTGGACCGA
  181 CCGGTGGTAC CTCACCCTTA CCGAGTCGGC GACACAGTGT GGGTCCGCCG ACACCAGACT
  241 AAGAACCTAG AACCTCGCTG GAAAGGACCT TACACAGTCC TGCTGACCAC CCCCACCGCC
  301 CTCAAAGTAG ACGGCATCGC AGCTTGGATA CACGCCGCCC ACGTGAAGGC TGCCGACCCC
  361 GGGGGTGGAC CATCCTCTAG ACTGCCGGAT CCCAGTGTGG TGGTACGGGA ATTCACACAG
  421 GACCTCACCA TGGGATGGAG CTGTATCATG CTCTTCTTGG CAGCAACAGC TACAGGTGTC
  481 CACTCCCAGG TCCAACTGCA GCAGCCTGGG GCTGAGCTTG TGAAGCCTGG GGCTTCAGTG
  541 AAGCTGTCCT GCAAGGCTTC TGGCTACACC TTCACCAGCT ACTTGATGCA CTGGGTGAAG
  601 CAGAGGCCTG GACGAGGCCT TGAGTGGATT GGAAGGATTG ATCCTAATAG TGGTGGTACT
  661 AAGTACAATG AGAAGTTCAA GAGCAAGGCC ACACTGACTG TAGACAAACC CTCCAGCACA
  721 GCCTACATGC AGCTCAGCAG CCTGACATCT GAGGACTCTG CGGTCTATTA TTGTGCAAGA
  781 TATGATTACT ACGGTAGTAG CTACTTTGAC TACTGGGGCC AAGGCACCAC TCTCACAGTC
  841 TCGAGTGCTA CAACAACAGC CCCATCTGTC TATCCCTTGG TCCCTGGCTG CGGTGACACA
  901 TCTGGATCCT CGGTGACACT GGGATGCCTT GTCAAAGGCT ACTTCCCTGA GCCGGTAACT
  961 GTAAAATGGA ACTATGGAGC CCTGTCCAGC GGTGTGCGCA CAGTCTCATC TGTACTGCAG
 1021 TCTGGGATTC TATTCCCCTC AGCAGCTTGG TGACTGTACC CCTCCAGCAC CTGGCCCCAG
1081 CAGACTGGTC AATCTGCCAA CGTAGCTCCA CCCCAGGCCA GCCAAAGG
//
Primer: IRES-R
Sequence file: RDB14503_A6Leb.seq check
>D04073A3_A6Le_1_IRES-R_C02_08_ABI24.ab1
    1 GCGTCAGCGG CTTCGGCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGGGCGGA
   61 ATTTACGTAG CGGCCGCCTA GGCACCTTGT CCAATCATGT TCCTGTAGTC AGGGATGGCC
  121 GTCTGCTTCA CCTGCACCAC TGAGGAGAAG ATCCACTTCA CCTTGAAGAG GGTGACAGAG
  181 GCGCTGTAGC ACACGCTGAG CAGGAAGAGG CTGATGAAGA TGGTGATGGT CGTCCAGAGC
  241 CCGTCCAGCT CCCCATCCTG GGCCTCAGCA CAGGTCTCAT TCAGTTCCAG CTCAGGGGAG
  301 CGAGACAGGT TCTTCTGTGT GTGGTGGTTA TGGAGAGCCT CATGCACCAC GGAGCAGGTA
  361 AAAATTTCTC CTTGCAACCA ACTGTCTGTA TCCACAGTGA GCTTGCTGTA GAGGAAGTAG
  421 GTCCCATCCG AGTCCAGGAT GGGTGGAGTG TTCTTGTAAT CCTGCTCCAG TTCTCCGTTC
  481 CTTTCCCACT CCACACTGAT GGCTTCAGAG AAGAAGTTGG TGACCAGGCA GGTCAAACTA
  541 ACCTTCTTCT TGGACATTTG TTCACGAGGT GGGGGTATGG TGTATACTTG AGGTGTCTGG
  601 GCTCTTCCTT TGGGTTTTGA GATGGTTCTC TCGATGGGGG CTGGGAGGGC TTTGTTGTTG
  661 ACCTTGCATT TGAACTCCTT GCCCCTCATC CAGTCCTGGT GCTGGATGGG GAGGGCACTG
  721 ACCACTCGGA AGGTACTGTT GTACTGAGCT TCACGGGGCT GCGTCCAGGC TGTGTGTACT
  781 TCTTTGTTGT CCACAAACCA GCTGACATGG ACATCTGGGT CATCCTCGCT CACATCCACC
  841 ACCACACACG TAACCTTGGG GGTTAGGGAG ATCATGAGTG CATCCTTGGG CTTTGGGGGG
  901 GAAGATGAAG ACGGATGGTC CACCCAAGAT GTTACCAGGG TGGGCATGAA GAACCCTGGG
  961 GGGGGTACTG GGCTTGGTAT TCTAGGCTCG ATTCTCTTGA TCAACTCAGT CTGGCTGGCT
 1021 GGGTGGCTAC GGTGGCAGAT GGACAGTCTG CTTGATAGTT GCTTGAGGTA CAGTCACAGC
 1081 TGCTGAGGAA TAGACCAGAC TGCAGTACGA TGAACTGTGG CGCACACGCT GGACAAGGGC
1141 CTTCCCT
//
Primer: IRES-F
Sequence file: RDB14503_A6Lec.seq check
>D04073A3_A6Le_1_IRES-F_C06_09_ABI24.ab1
    1 CTTCAAACCT TTCATGTGTT TAGTCGAGGT TAAAAAACGT CTAGGCCCCC CGAACCACGG
   61 GGACGTGGTT TTCCTTTGAA AAACACGATG ATAATATGGC CACAACCATG GTGAGCAAGG
  121 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG
  181 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC
  241 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC
  301 TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT
  361 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG
  421 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG
  481 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA
  541 ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA
  601 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC
  661 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC
  721 AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG
  781 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAAGC GGCCGGCCGC
  841 CAGCACAGTG GTCGACGATA AAATAAAAGA TTTTATTTAG TCTCCAGAAA AAGGGGGGGA
  901 ATGAAAGACC CCACCTGTAG GTTTGGCAAG CTAGCTTAAG TAACGCCATT TTTGCAAGGG
  961 CATGGGAAAA ATACATAACT GAGAATAGAG AAAGTTCCAG GATCAAGGTC AGGAACAGGA
 1021 TGGGAACAGC CTGAATATGG GGCCAACAGA TATCTGTGGT AGCAGTTCCT TGCCCCGGCT
1081 CAAGGCTAGG ACCAGAGTGG CAACAGCCTG AAATATAATT GGGACCTCAA AC
//
Primer: Amp_R
Sequence file: RDB14503_A6Led.seq check
>D04073A3_A6Le_1_Amp_R_D06_12_ABI24.ab1
    1 CTCGTTTTAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
   61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGCATAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTG
  181 CAAGGCATGG AAAAATACAT AACTGAGAAT AGAAAAGTTC AGATCAAGGT CAGGAACAGA
  241 TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC
  301 AGGGCCAAGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG
  361 TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCA GCCCTCAGCA
  421 GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC
  481 CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG
  541 AGCTCAATAA AAGAGCCCAC AACCCCTCAC TCGGCGCGCC AGTCCTCCGA TTGACTGAGT
  601 CGCCCGGGTA CCCGTGTATC CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG
  661 CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC GTCAGCGGGG GTCTTTCATT
  721 TGGGGGCTCG TCCGGGATCG GGAGACCCCT GCCCAGGGAC CACCGACCCA CCACCGGGAG
  781 GTAAGCTGGC CAGCAACTTA TCTGTGTCTG TCCGATTGTC TAGTGTCTAT GACTGATTTT
  841 ATGCGCCTGC GTCGGTACTA GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC CCGTGGTGGA
  901 ACTGACGAGT TCGGAACACC CGGCCGCAAC CCTGGGAGAC GTCCCAGGGA CTTCTGGGGC
  961 CGTTTTTGTG GCCCGACCTG AGTCCAAAAA TCCCGATCGT TTTTGGGACT CTTTGGGTGC
 1021 AACCCCCCCT TTAGAAGAGG ATATGTTGGA TCCTTGTTAA GAGACGAAGG AACTAAACAG
1081 ATCCGGCCTT CGATCCTGAA GTTTTTGGCA TTTTCCAGT
//
Primer: SV40pA3'element Reverse
Sequence file: RDB14503_A6Lee.seq check
>D04073A3_A6Le_1_SV40pA3element Reverse_E06_15_ABI24.ab1
    1 GTCGCAGAGG GCGGACCTAT GGAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT
   61 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC
  121 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG
  181 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT
  241 GGCCGATTCA TTAATGCAAC TATGGCCATT TAATGTAAAT ACTTAAGAAA AAAAACCAAA
  301 TTAATTTTGA TACATGCTGC ATGTGAAAGA CCCCCGCTGA CGGGTAGTCA ATCACTCAGA
  361 GGAGACCCTC CCAAGGAACA GCGAGACCAC AAGTCGGATG CAACTGCAAG AGGGTTTATT
  421 GGATACACGG GTACCCGGGC GACTCAGTCA ATCGGAGGAC TGGCGCCCCG AGTGAGGGGT
  481 TGTGGGCTCT TTTATTGAGC TCGGGGAGCA GAAGCGCGCG AACAGAAGCG AGAAGCGAAC
  541 TGATTGGTTA GTTCAAATAA GGCACAGGGT CATTTCAGGT CCTTGGGGCA CCCTGGAAAC
  601 ATCTGATGGT TCTCTAGAAA CTGCTGAGGG CTGGACCGCA TCTGGGGACC ATCTGTTCTT
  661 GGCCCTGAGC CGGGGCAGGA ACTGCTTACC ACAGATATCC TGTTTGGCCC ATATTCAGCT
  721 GTTCCATCTG TTCTTGGCCC TGAGCCGGGG CAGGAACTGC TTACCACAGA TATCCTGTTT
  781 GGCCCATATT CAGCTGTTCC ATCTGTTCCT GACCTTGATC TGAACTTCTC TATTCTCAGT
  841 TATGTATTTT TTCCATGCCT TGCAAAATGG CGTTACTTAA GCTAGCTTGC CAAACCTACA
  901 GGTGGGGGTC TTTCATTCCC CCCCTTTTTT CTGGAGACTA AATAAAATCT TTTTATTTTT
  961 ATCGTCGACC CACTGTGCTG GGCGGCCCGG CCCGCTTTTA CTTTGTACAG CTCGTCCATG
 1021 CCGAGAGTTG ATCCCGGCGC GGTCACGAAC TCCAAGCAGA CCATGTGATT CGCGCTTCTC
1081 GTTGGGGATC TTTGGCTCAG GTCGGAACT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]