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pCMV-NICD 3xFLAG (#RDB14500)

Expression vector of mouse Notch1 active form N1deltaECD.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Sasaki, N., Development 138 (1): 55-64 (2011). PMID 21098559. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name pYY554-1, N1deltaECD
Clone info. Expression vector of mouse Notch1 active form. N1deltaECD in the Fig.4 of Sasaki, N., Development 138 (1); 55-64, 2011.
Comment Nucleotide sequence of 5372 to 7857nt of NM_008714.3 (1..9497nt) corresponding Ile of 1704aa to 2531aa in Fig.1 of Kato, H., FEBS Lett., 395, 221-224 (1996). Known difference: nucleotide substitution of G to A at 7159nt of NM_008714.3 (Glu at 2299aa to Lys).
Vector backbone p3xFLAG-CMV-14 (Plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Mouse Notch gene homolog 1 cDNA
Depositor|Developer Saga, Yumiko |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Sasaki, N., Development 138, 55-64, 2011) and an acknowledgment to the DEPOSITOR are requested.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sasaki, N., Development 138, 55-64, 2011)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

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RDB14500 pCMV-NICD 3xFLAG DNA solution

How to cite this biological resource

Materials & Methods section:

The pCMV-NICD 3xFLAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14500).

Reference section:

Sasaki, N., Kiso, M., Kitagawa, M., Saga, Y., The repression of Notch signaling occurs via the destabilization of mastermind-like 1 by Mesp2 and is essential for somitogenesis. Development 138 (1): 55-64 (2011). PMID 21098559. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Sasaki, N., The repression of Notch signaling occurs via the destabilization of mastermind-like 1 by Mesp2 and is essential for somitogenesis. Development 138 (1): 55-64 (2011). PMID 21098559. [PubMed] [Article] [RRC of NBRP]