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BW25113-based RFzero-iy

Escherichia coli host strain to produce non-natural amino acids containing proteins.

Catalog number RDB14427
Resource name BW25113-based RFzero-iy
Clone info. Escherichia coli BW25113-based host strain with the UAG codon reassigned to 3-iodotyrosine.
BW25113: rrnB DElacZ4787 HsdR514 DE(araBAD)567 DE(rhaBAD)568 rph-1; RFzero-iy: prfA::zeo, BAC8(cat), piodoTyrRS-MJR1-gent(pACYC)
Depositor Sakamoto, Kensaku |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Mukai, T., Biochem. Biophys. Res. Commun. 411(4):757-761, 2011) and an acknowledgment to the DEPOSITOR is requested. The materials are only available based on material transfer agreement between the user and the depositor. The materials are only available for the user belonging to a nonprofit organization.
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Remarks ((Additional form)) Approval Form(FormD)
Recombinant E. coli strain.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14427 BW25113-based RFzero-iy Bacteria in stub agar

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file PDF RDB14427z.pdf from Depositor

Featured content

Featured content Incorporation of synthetic amino acids into proteins at specific sites [link] (English text)
Featured content Dnaconda's recommendation EXR0072e (English text)
Featured content Bacteria host strains (en) (English text)
Featured content Bacteria host strains (ja) (Japanese text)
Featured content Incorporation of synthetic amino acids into proteins at specific sites [link] (Japanese text)

References

original Mukai, T., Genetic-code evolution for protein synthesis with non-natural amino acids. Biochem. Biophys. Res. Commun. 411 (4): 757-761 (2011). PMID 21782790.

User reports and related articles (go to bottom)


Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

References

original Mukai, T., Genetic-code evolution for protein synthesis with non-natural amino acids. Biochem. Biophys. Res. Commun. 411 (4): 757-761 (2011). PMID 21782790.
reference Ohtake, K., Protein stabilization utilizing a redefined codon. Sci. Rep. 5: 9762 (2015). PMID 25985257.
reference Ohtake, K., Efficient decoding of the UAG triplet as a full-fledged sense codon enhances the growth of a prfA-deficient strain of Escherichia coli. J. Bacteriol. 194 (10): 2606-2613 (2012). PMID 22427623.
reference Mukai, T., Codon reassignment in the Escherichia coli genetic code. Nucleic Acids Res. 38 (22): 8188-8195 (2010). PMID 20702426.

2018.09.20

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