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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

BW25113-based RFzero-iy

Escherichia coli host strain to produce non-natural amino acids containing proteins.

Catalog number RDB14427
Resource name BW25113-based RFzero-iy
Clone info. Escherichia coli BW25113-based host strain with the UAG codon reassigned to 3-iodotyrosine.
Comment BW25113: rrnB DElacZ4787 HsdR514 DE(araBAD)567 DE(rhaBAD)568 rph-1; RFzero-iy: prfA::zeo, BAC8(cat), piodoTyrRS-MJR1-gent(pACYC)
Growth remarks Please note the requirement of culture medium composition (see PDF). It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.
Depositor|Developer Sakamoto, Kensaku |
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            Reference sequence
              
             
            Remarks, protocol and/or map (pdf) RDB14427z.pdf

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Mukai, T., Biochem. Biophys. Res. Commun. 411 (4):757-761, 2011) and an acknowledgment to the DEPOSITOR is requested.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks Recombinant E. coli strain. It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって、寄託者の指定する文献の引用 (Mukai, T., Biochem. Biophys. Res. Commun. 411 (4):757-761, 2011) ならびに謝辞の表明を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            備考 【追加提供依頼書】受入れ確認書(書式J)
            大腸菌組換え体リソースです。
            実験を行う前に、適切な寒天プレート上でコロニーを単離し、培養することを推奨します。
            組換え体提供にかかる書式が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB14427 BW25113-based RFzero-iy Bacteria in stab agar

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The BW25113-based RFzero-iy was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14427).

            Reference section:

            Mukai, T., Yanagisawa, T., Ohtake, K., Wakamori, M., Adachi, J., Hino, N., Sato, A., Kobayashi, T., Hayashi, A., Shirouzu, M., Umehara, T., Yokoyama, S., Sakamoto, K., Genetic-code evolution for protein synthesis with non-natural amino acids. Biochem. Biophys. Res. Commun. 411 (4): 757-761 (2011). PMID 21782790.

            Further references such as user reports and related articles (go to bottom)

            References and tips

            Featured content

            Featured content Incorporation of synthetic amino acids into proteins at specific sites [link] (English text)
            Featured content Dnaconda's recommendation EXR0072e (English text)
            Featured content Bacteria host strains (en) (English text)
            Featured content Bacteria host strains (ja) (Japanese text)
            Featured content Incorporation of synthetic amino acids into proteins at specific sites [link] (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14427_A9H6p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Mukai, T., Genetic-code evolution for protein synthesis with non-natural amino acids. Biochem. Biophys. Res. Commun. 411 (4): 757-761 (2011). PMID 21782790.
            reference Ohtake, K., Protein stabilization utilizing a redefined codon. Sci. Rep. 5: 9762 (2015). PMID 25985257.
            reference Ohtake, K., Efficient decoding of the UAG triplet as a full-fledged sense codon enhances the growth of a prfA-deficient strain of Escherichia coli. J. Bacteriol. 194 (10): 2606-2613 (2012). PMID 22427623.
            reference Mukai, T., Codon reassignment in the Escherichia coli genetic code. Nucleic Acids Res. 38 (22): 8188-8195 (2010). PMID 20702426.

            2023.05.12

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