RDB14427 - BW25113-based RFzero-iy

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BW25113-based RFzero-iy

Escherichia coli host strain to produce non-natural amino acids containing proteins.

Catalog number	RDB14427
Resource name	BW25113-based RFzero-iy
Clone info.	Escherichia coli BW25113-based host strain with the UAG codon reassigned to 3-iodotyrosine. BW25113: rrnB DElacZ4787 HsdR514 DE(araBAD)567 DE(rhaBAD)568 rph-1; RFzero-iy: prfA::zeo, BAC8(cat), piodoTyrRS-MJR1-gent(pACYC)
Growth remarks	Please note the requirement of culture medium composition (see PDF). It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.
Depositor\|Developer	Sakamoto, Kensaku \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Mukai, T., Biochem. Biophys. Res. Commun. 411(4):757-761, 2011) and an acknowledgment to the DEPOSITOR is requested. The materials are only available based on material transfer agreement between the user and the depositor. The materials are only available for the user belonging to a nonprofit organization.

Ordering Please visit Information of Request for Distribution.[link]
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Approval form by depositor [Word]

Remarks ((Additional form)) Approval Form(FormD)
Recombinant E. coli strain. It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.
Please obtain written approval of the depositor.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Mukai, T., Biochem. Biophys. Res. Commun. 411(4):757-761, 2011) and an acknowledgment to the DEPOSITOR is requested. The materials are only available based on material transfer agreement between the user and the depositor. The materials are only available for the user belonging to a nonprofit organization.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Approval form by depositor [Word]
Remarks	((Additional form)) Approval Form(FormD) Recombinant E. coli strain. It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments. Please obtain written approval of the depositor.

提供条件	利用者は、研究成果の公表にあたって、寄託者の指定する文献の引用 (Mukai, T., Biochem. Biophys. Res. Commun. 411(4):757-761, 2011) ならびに謝辞の表明を必要とする。利用者は寄託者から事前に提供承諾書を取得すること。非営利機関の利用者に限定する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 [Word] 遺伝子組換え生物の受入れ確認書 [Word] 寄託者による提供承諾書 [Word]
備考	【追加提供依頼書】承諾書(FormD), 受入れ確認書(書式J) 大腸菌組換え体リソースです。実験を行う前に、適切な寒天プレート上でコロニーを単離し、培養することを推奨します。組換え体提供にかかる書式が必要です。寄託者の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14427	BW25113-based RFzero-iy	Bacteria in stab agar

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file	Remarks, protocol and/or map (pdf) RDB14427z.pdf

Original reference

original	Mukai, T., Genetic-code evolution for protein synthesis with non-natural amino acids. Biochem. Biophys. Res. Commun. 411 (4): 757-761 (2011). PMID 21782790.

Further references such as user reports and related articles (go to bottom)

Featured content	Incorporation of synthetic amino acids into proteins at specific sites [link] (English text)
Featured content	Dnaconda's recommendation EXR0072e (English text)
Featured content	Bacteria host strains (en) (English text)
Featured content	Bacteria host strains (ja) (Japanese text)
Featured content	Incorporation of synthetic amino acids into proteins at specific sites [link] (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14427_A9H6p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Mukai, T., Genetic-code evolution for protein synthesis with non-natural amino acids. Biochem. Biophys. Res. Commun. 411 (4): 757-761 (2011). PMID 21782790.
reference	Ohtake, K., Protein stabilization utilizing a redefined codon. Sci. Rep. 5: 9762 (2015). PMID 25985257.
reference	Ohtake, K., Efficient decoding of the UAG triplet as a full-fledged sense codon enhances the growth of a prfA-deficient strain of Escherichia coli. J. Bacteriol. 194 (10): 2606-2613 (2012). PMID 22427623.
reference	Mukai, T., Codon reassignment in the Escherichia coli genetic code. Nucleic Acids Res. 38 (22): 8188-8195 (2010). PMID 20702426.

2020.10.02

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