RDB14427 - BW25113-based RFzero-iy

Resource data sheet
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BW25113-based RFzero-iy

Escherichia coli host strain to produce non-natural amino acids containing proteins.

Catalog number	RDB14427
Resource name	BW25113-based RFzero-iy
Clone info.	Escherichia coli BW25113-based host strain with the UAG codon reassigned to 3-iodotyrosine. BW25113: rrnB DElacZ4787 HsdR514 DE(araBAD)567 DE(rhaBAD)568 rph-1; RFzero-iy: prfA::zeo, BAC8(cat), piodoTyrRS-MJR1-gent(pACYC)
Depositor	Sakamoto, Kensaku \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Mukai, T., Biochem. Biophys. Res. Commun. 411(4):757-761, 2011) and an acknowledgment to the DEPOSITOR is requested. The materials are only available based on material transfer agreement between the user and the depositor. The materials are only available for the user belonging to a nonprofit organization.
(Japanese text [open/close])

利用者は、研究成果の公表にあたって、寄託者の指定する文献の引用 (Mukai, T., Biochem. Biophys. Res. Commun. 411(4):757-761, 2011) ならびに謝辞の表明を必要とする。利用者は寄託者から事前に提供承諾書を取得すること。非営利機関の利用者に限定する。

Remarks ((Additional form)) Approval Form(FormD)
Recombinant E. coli strain.
(Japanese text [open/close])

【追加提供依頼書】承諾書(FormD), 受入れ確認書(書式J)
大腸菌組換え体リソースです。
組換え体提供にかかる書式が必要です [link]。

Catalog # Resource name Shipping form Fee (non-profit org.)

RDB14427 BW25113-based RFzero-iy Bacteria in stub agar

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Mukai, T., Biochem. Biophys. Res. Commun. 411(4):757-761, 2011) and an acknowledgment to the DEPOSITOR is requested. The materials are only available based on material transfer agreement between the user and the depositor. The materials are only available for the user belonging to a nonprofit organization. (Japanese text [open/close]) 利用者は、研究成果の公表にあたって、寄託者の指定する文献の引用 (Mukai, T., Biochem. Biophys. Res. Commun. 411(4):757-761, 2011) ならびに謝辞の表明を必要とする。利用者は寄託者から事前に提供承諾書を取得すること。非営利機関の利用者に限定する。
Remarks	((Additional form)) Approval Form(FormD) Recombinant E. coli strain. (Japanese text [open/close]) 【追加提供依頼書】承諾書(FormD), 受入れ確認書(書式J) 大腸菌組換え体リソースです。組換え体提供にかかる書式が必要です [link]。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14427	BW25113-based RFzero-iy	Bacteria in stub agar

References and tips

Electronic file

Electronic file	PDF RDB14427z.pdf from Depositor

Featured content	Incorporation of synthetic amino acids into proteins at specific sites [link] (English text)
Featured content	Dnaconda's recommendation EXR0072e (English text)
Featured content	Bacteria host strains (en) (English text)
Featured content	Bacteria host strains (ja) (Japanese text)
Featured content	Incorporation of synthetic amino acids into proteins at specific sites [link] (Japanese text)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References

original	Mukai, T., Genetic-code evolution for protein synthesis with non-natural amino acids. Biochem. Biophys. Res. Commun. 411 (4): 757-761 (2011). PMID 21782790.
reference	Ohtake, K., Protein stabilization utilizing a redefined codon. Sci. Rep. 5: 9762 (2015). PMID 25985257.
reference	Ohtake, K., Efficient decoding of the UAG triplet as a full-fledged sense codon enhances the growth of a prfA-deficient strain of Escherichia coli. J. Bacteriol. 194 (10): 2606-2613 (2012). PMID 22427623.
reference	Mukai, T., Codon reassignment in the Escherichia coli genetic code. Nucleic Acids Res. 38 (22): 8188-8195 (2010). PMID 20702426.

2018.07.05

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