Escherichia coli host strain to produce non-natural amino acids containing proteins.
| Catalog number | RDB14427 |
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| Resource name | BW25113-based RFzero-iy |
| Clone info. | Escherichia coli BW25113-based host strain with the UAG codon reassigned to 3-iodotyrosine. BW25113: rrnB DElacZ4787 HsdR514 DE(araBAD)567 DE(rhaBAD)568 rph-1; RFzero-iy: prfA::zeo, BAC8(cat), piodoTyrRS-MJR1-gent(pACYC) |
| Depositor | Sakamoto, Kensaku | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Mukai, T., Biochem. Biophys. Res. Commun. 411(4):757-761, 2011) and an acknowledgment to the DEPOSITOR is requested. The materials are only available based on material transfer agreement between the user and the depositor. The materials are only available for the user belonging to a nonprofit organization. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Approval form by depositor [Word] |
| Remarks | ((Additional form)) Approval Form(FormD) Recombinant E. coli strain. Please obtain written approval of the depositor. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB14427 | BW25113-based RFzero-iy | Bacteria in stub agar |
Electronic file
| Electronic file | PDF RDB14427z.pdf from Depositor |
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Featured content
| Featured content | Incorporation of synthetic amino acids into proteins at specific sites [link] (English text) |
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| Featured content | Dnaconda's recommendation EXR0072e (English text) |
| Featured content | Bacteria host strains (en) (English text) |
| Featured content | Bacteria host strains (ja) (Japanese text) |
| Featured content | Incorporation of synthetic amino acids into proteins at specific sites [link] (Japanese text) |
References
| original | Mukai, T., Genetic-code evolution for protein synthesis with non-natural amino acids. Biochem. Biophys. Res. Commun. 411 (4): 757-761 (2011). PMID 21782790. |
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User reports and related articles (go to bottom)
This clone will be sequenced a portion and digested by restriction enzyme for examination.
References
| original | Mukai, T., Genetic-code evolution for protein synthesis with non-natural amino acids. Biochem. Biophys. Res. Commun. 411 (4): 757-761 (2011). PMID 21782790. |
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| reference | Ohtake, K., Protein stabilization utilizing a redefined codon. Sci. Rep. 5: 9762 (2015). PMID 25985257. |
| reference | Ohtake, K., Efficient decoding of the UAG triplet as a full-fledged sense codon enhances the growth of a prfA-deficient strain of Escherichia coli. J. Bacteriol. 194 (10): 2606-2613 (2012). PMID 22427623. |
| reference | Mukai, T., Codon reassignment in the Escherichia coli genetic code. Nucleic Acids Res. 38 (22): 8188-8195 (2010). PMID 20702426. |
2018.09.20
GNP_filter3_RDBDEP_html_180823.pl