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BW25113-based RFzero-iy (#RDB14427)

Escherichia coli host strain to produce non-natural amino acids containing proteins.

Clone info. Escherichia coli BW25113-based host strain with the UAG codon reassigned to 3-iodotyrosine.
Comment BW25113: rrnB DElacZ4787 HsdR514 DE(araBAD)567 DE(rhaBAD)568 rph-1; RFzero-iy: prfA::zeo, BAC8(cat), piodoTyrRS-MJR1-gent(pACYC)
Growth remarks Please note the requirement of culture medium composition (see PDF). It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.
Depositor|Developer Sakamoto, Kensaku |
 
Remarks, protocol and/or map (pdf) RDB14427z.pdf

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Mukai, T., Biochem. Biophys. Res. Commun. 411 (4):757-761, 2011) and an acknowledgment to the DEPOSITOR is requested.
Remarks Recombinant E. coli strain. It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって、寄託者の指定する文献の引用 (Mukai, T., Biochem. Biophys. Res. Commun. 411 (4):757-761, 2011) ならびに謝辞の表明を必要とする。
備考 【追加提供依頼書】受入れ確認書(書式J)
大腸菌組換え体リソースです。
実験を行う前に、適切な寒天プレート上でコロニーを単離し、培養することを推奨します。
組換え体提供にかかる書式が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14427 BW25113-based RFzero-iy Bacteria in stab agar

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The BW25113-based RFzero-iy was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14427).

Reference section:

Mukai, T., Yanagisawa, T., Ohtake, K., Wakamori, M., Adachi, J., Hino, N., Sato, A., Kobayashi, T., Hayashi, A., Shirouzu, M., Umehara, T., Yokoyama, S., Sakamoto, K., Genetic-code evolution for protein synthesis with non-natural amino acids. Biochem. Biophys. Res. Commun. 411 (4): 757-761 (2011). PMID 21782790. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14427_A9H6p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Mukai, T., Genetic-code evolution for protein synthesis with non-natural amino acids. Biochem. Biophys. Res. Commun. 411 (4): 757-761 (2011). PMID 21782790. [link to RRC of NBRP]
reference Ohtake, K., Protein stabilization utilizing a redefined codon. Sci. Rep. 5: 9762 (2015). PMID 25985257.
reference Ohtake, K., Efficient decoding of the UAG triplet as a full-fledged sense codon enhances the growth of a prfA-deficient strain of Escherichia coli. J. Bacteriol. 194 (10): 2606-2613 (2012). PMID 22427623.
reference Mukai, T., Codon reassignment in the Escherichia coli genetic code. Nucleic Acids Res. 38 (22): 8188-8195 (2010). PMID 20702426.