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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCIneoEGFPC2

Plasmid vector for constructing GFP-tagged expression vector.

Catalog number RDB14401
Resource name pCIneoEGFPC2
Clone info. Plasmid vector for constructing GFP-tagged expression vector.
Comment NheI fragment of EGFP-C2 (Clontech) was ligated into corresponding site of pCl-neo to generate this vector.
Vector backbone pCI-neo (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Hata, Yutaka |
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External Database
Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nagashima, S. et al. Cancer Sci. 107 (6), 791-802, 2016) and an acknowledgment to the DEPOSITOR are requested.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nagashima, S. et al. Cancer Sci. 107 (6), 791-802, 2016) を引用し、寄託者への謝辞の表明を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB14401 pCIneoEGFPC2 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Nagashima, S., Validation of chemical compound library screening for transcriptional co-activator with PDZ-binding motif inhibitors using GFP-fused transcriptional co-activator with PDZ-binding motif. Cancer Sci. 107 (6): 791-802 (2016). PMID 27009852.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14401_A6Kip1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward
            Region: CMV pro,chineric intron,T7 pro,EGFP 5'
            Sequence file: RDB14401_A6Kia.seq check
            >D04088A7_A6Ki_1_CMV-Forward_A01_01_ABI08.ab1
                1 NNNNNNNNNN NNNGCNNGCC CNGTTGNNGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG
               61 TCTATATAAG CAGAGCTCGT TTAGTGAACC GTCAGATCAC TAGAAGCTTT ATTGCGGTAG
              121 TTTATCACAG TTAAATTGCT AACGCAGTCA GTGCTTCTGA CACAACAGTC TCGAACTTAA
              181 GCTGCAGTGA CTCTCTTAAG GTAGCCTTGC AGAAGTTGGT CGTGAGGCAC TGGGCAGGTA
              241 AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT GTCGAGACAG
              301 AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA CTTTGCCTTT
              361 CTCTCCACAG GTGTCCACTC CCAGTTCAAT TACAGCTCTT AAGGCTAGAG TACTTAATAC
              421 GACTCACTAT AGGCTAGCGC TACCGGTCGC CACCATGGTG AGCAAGGGCG AGGAGCTGTT
              481 CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG
              541 CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG
              601 CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT
              661 GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT
              721 GCCCGAANGC TACGTCCAGG ANCGCACCAT CTTCTTCAAN GACGACGGCA ACTACNAGAN
              781 CCGCGCCGAN GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCNANC TGAANGGCAT
              841 CNACTTCNAN GANGANNGCA ACATCCTGGG GCACAAGCTG GNNTANNACT ACNNCAGCCA
              901 CNNCGTCTAT ATCANGGCCN ANANGCAGAN NAACNGNNTN NANGNNGNAC TTCNANATNC
            961 NNNNNNNCAT CNNNNNNNNN ANCNNGCNNC NNNNNNACNN NNNNNNNN
            //
            Primer: pAxCALNLF1
            Region: EGFP 3'
            Sequence file: RDB14401_A6Kib.seq check
            >D04088A7_A6Ki_1_pAxCALNLF1_B01_02_ABI08.ab1
                1 NNNNNNNNNN NNNANNNNGT CTGNTCGNNG CNNTAACCCT CACTAAAGGG AAGCGGCCGC
               61 CCGGGTCGAC TCTAGAGGTA CCACGCGTGA ATTCGAAGCT TGAGCTCGAG ATCTGAGTCC
              121 GGCCGGACTT GTACAGCTCG TCCATGCCGA GAGTGATCCC GGCGGCGGTC ACGAACTCCA
              181 GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT CAGGGCGGAC TGGGTGCTCA
              241 GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT GGGGGTGTTC TGCTGGTAGT
              301 GGTCGGCGAG CTGCACGCTG CCGTCCTCGA TGTTGTGGCG GATCTTGAAG TTCACCTTGA
              361 TGCCGTTCTT CTGCTTGTCG GCCATGATAT AGACGTTGTG GCTGTTGTAG TTGTACTCCA
              421 GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC TCGATGCGGT
              481 TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT
              541 TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT GGCGGACTTG AAGAAGTCGT
              601 GCTGCTTCAT GTGGTCGGGG TAGCGGCTGA AGCACTGCAC GCCGTANGTC AGGGTGGTCA
              661 CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA GATGAACTTC AGGGTCAGCT
              721 TGCCGTANGT GGCATCGCCC TCGCCCTCGC CGGANACGCT GAACTTGTGN CCGTTTACGT
              781 CGCCGTCCAG CTCGACCAGG ANGGGCACCA CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA
              841 CCATGGTGGC GACCGGTAGC GCTAGNNTAT ANTGAGTCGN ANTAAGTACT CTANCCTTNA
            901 GANCTGNNAT TGNACTGGGN NNNNNNNNN
            //
            Primer: SV40pro_F_V2
            Region: NeoR
            Sequence file: RDB14401_A6Kic.seq check
            >D04088A7_A6Ki_1_SV40pro_F_V2_H02_08_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNGCTTTT GNNANNNGCT TGNTTCTTCT GACACAACAG
               61 TCTCGAACTT AAGGCTNNNN NCACCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC
              121 GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC
              181 TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA
              241 CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC
              301 GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT
              361 GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA
              421 AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC
              481 ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT
              541 TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC
              601 CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG
              661 CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT
              721 GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT
              781 TGGCGGCGAA TGGGCTGANC GCTTCCTCGT GCTTTACNNT ATCGCCGCTC CCGATTCGCA
              841 GCGCATCGCC TTCTATCGCC TTCTTGACGA NTTCTNCTGA NCNGGNNTCT GGGGTTCGAA
              901 ATGANCGANN NAGCGANNNC NNNNCNNNNC NTCACGATNG NCNGCNATAA AATATCTTTA
            961 TTTTCATTAC ATNTGNNNNN NNGNNTTTTT NGNGNNNNNN NNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Nagashima, S., Validation of chemical compound library screening for transcriptional co-activator with PDZ-binding motif inhibitors using GFP-fused transcriptional co-activator with PDZ-binding motif. Cancer Sci. 107 (6): 791-802 (2016). PMID 27009852.

            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl