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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCIneoEGFPC2 (#RDB14401)

Plasmid vector for constructing GFP-tagged expression vector.

Clone info. Plasmid vector for constructing GFP-tagged expression vector.
Comment NheI fragment of EGFP-C2 (Clontech) was ligated into corresponding site of pCl-neo to generate this vector.
Vector backbone pCI-neo (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Growth conditions LB+Amp, 37oC
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Hata, Yutaka |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nagashima, S. et al. Cancer Sci. 107 (6), 791-802, 2016) and an acknowledgment to the DEPOSITOR are requested.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nagashima, S. et al. Cancer Sci. 107 (6), 791-802, 2016) を引用し、寄託者への謝辞の表明を必要とする。

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RDB14401 pCIneoEGFPC2 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCIneoEGFPC2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14401).

Reference section:

Nagashima, S., Maruyama, J., Kawano, S., Iwasa, H., Nakagawa, K., Ishigami-Yuasa, M., Kagechika, H., Nishina, H., Hata, Y., Validation of chemical compound library screening for transcriptional co-activator with PDZ-binding motif inhibitors using GFP-fused transcriptional co-activator with PDZ-binding motif. Cancer Sci. 107 (6): 791-802 (2016). PMID 27009852. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14401_A6Kip1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,chineric intron,T7 pro,EGFP 5'
Sequence file: RDB14401_A6Kia.seq check
>D04088A7_A6Ki_1_CMV-Forward_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNGCNNGCC CNGTTGNNGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG
   61 TCTATATAAG CAGAGCTCGT TTAGTGAACC GTCAGATCAC TAGAAGCTTT ATTGCGGTAG
  121 TTTATCACAG TTAAATTGCT AACGCAGTCA GTGCTTCTGA CACAACAGTC TCGAACTTAA
  181 GCTGCAGTGA CTCTCTTAAG GTAGCCTTGC AGAAGTTGGT CGTGAGGCAC TGGGCAGGTA
  241 AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT GTCGAGACAG
  301 AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA CTTTGCCTTT
  361 CTCTCCACAG GTGTCCACTC CCAGTTCAAT TACAGCTCTT AAGGCTAGAG TACTTAATAC
  421 GACTCACTAT AGGCTAGCGC TACCGGTCGC CACCATGGTG AGCAAGGGCG AGGAGCTGTT
  481 CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG
  541 CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG
  601 CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT
  661 GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT
  721 GCCCGAANGC TACGTCCAGG ANCGCACCAT CTTCTTCAAN GACGACGGCA ACTACNAGAN
  781 CCGCGCCGAN GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCNANC TGAANGGCAT
  841 CNACTTCNAN GANGANNGCA ACATCCTGGG GCACAAGCTG GNNTANNACT ACNNCAGCCA
  901 CNNCGTCTAT ATCANGGCCN ANANGCAGAN NAACNGNNTN NANGNNGNAC TTCNANATNC
961 NNNNNNNCAT CNNNNNNNNN ANCNNGCNNC NNNNNNACNN NNNNNNNN
//
Primer: pAxCALNLF1 (Pr0166)
Region: EGFP 3'
Sequence file: RDB14401_A6Kib.seq check
>D04088A7_A6Ki_1_pAxCALNLF1_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNANNNNGT CTGNTCGNNG CNNTAACCCT CACTAAAGGG AAGCGGCCGC
   61 CCGGGTCGAC TCTAGAGGTA CCACGCGTGA ATTCGAAGCT TGAGCTCGAG ATCTGAGTCC
  121 GGCCGGACTT GTACAGCTCG TCCATGCCGA GAGTGATCCC GGCGGCGGTC ACGAACTCCA
  181 GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT CAGGGCGGAC TGGGTGCTCA
  241 GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT GGGGGTGTTC TGCTGGTAGT
  301 GGTCGGCGAG CTGCACGCTG CCGTCCTCGA TGTTGTGGCG GATCTTGAAG TTCACCTTGA
  361 TGCCGTTCTT CTGCTTGTCG GCCATGATAT AGACGTTGTG GCTGTTGTAG TTGTACTCCA
  421 GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC TCGATGCGGT
  481 TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT
  541 TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT GGCGGACTTG AAGAAGTCGT
  601 GCTGCTTCAT GTGGTCGGGG TAGCGGCTGA AGCACTGCAC GCCGTANGTC AGGGTGGTCA
  661 CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA GATGAACTTC AGGGTCAGCT
  721 TGCCGTANGT GGCATCGCCC TCGCCCTCGC CGGANACGCT GAACTTGTGN CCGTTTACGT
  781 CGCCGTCCAG CTCGACCAGG ANGGGCACCA CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA
  841 CCATGGTGGC GACCGGTAGC GCTAGNNTAT ANTGAGTCGN ANTAAGTACT CTANCCTTNA
901 GANCTGNNAT TGNACTGGGN NNNNNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR
Sequence file: RDB14401_A6Kic.seq check
>D04088A7_A6Ki_1_SV40pro_F_V2_H02_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGCTTTT GNNANNNGCT TGNTTCTTCT GACACAACAG
   61 TCTCGAACTT AAGGCTNNNN NCACCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC
  121 GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC
  181 TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA
  241 CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC
  301 GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT
  361 GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA
  421 AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC
  481 ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT
  541 TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC
  601 CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG
  661 CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT
  721 GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT
  781 TGGCGGCGAA TGGGCTGANC GCTTCCTCGT GCTTTACNNT ATCGCCGCTC CCGATTCGCA
  841 GCGCATCGCC TTCTATCGCC TTCTTGACGA NTTCTNCTGA NCNGGNNTCT GGGGTTCGAA
  901 ATGANCGANN NAGCGANNNC NNNNCNNNNC NTCACGATNG NCNGCNATAA AATATCTTTA
961 TTTTCATTAC ATNTGNNNNN NNGNNTTTTT NGNGNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nagashima, S., Validation of chemical compound library screening for transcriptional co-activator with PDZ-binding motif inhibitors using GFP-fused transcriptional co-activator with PDZ-binding motif. Cancer Sci. 107 (6): 791-802 (2016). PMID 27009852. [link to RRC of NBRP]