Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPmatLuc1

Luciferase of Pyrocoelia matsumurai firefly with higher luminous intensity.

Catalog number RDB14359
Resource name pPmatLuc1
Clone info. Luciferase of Pyrocoelia matsumurai firefly with higher luminous intensity. pPmatLuc1 is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Pyrocoelia matsumurai Luciferase cDNA
Depositor|Developer OLYMPUS CORPORATION, | Ogoh, Katsunori |
Other clones in our bank

External Database
Pyrocoelia matsumurai Luciferase

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Akiyoshi R, Ogoh K, Suzuki H. 2012. Firefly luciferase. Japan patent publication 2012-235756). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (ホタル由来ルシフェラーゼ 特開2012-235756)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは,本件リソースが製品の一部に組み込まれる場合,本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては,営利,非営利機関等の利用者制限は定めない。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14359 pPmatLuc1 DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Akiyoshi, R., Firefly luciferase. Japan Patent Abstracts 2012-235756.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Higher intensity luciferase (English text)
          Featured content Higher intensity luciferase (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14359_A6Jcp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: Reverse2
          Sequence file: RDB14359_A6Jca.seq check
          >D03707A1_A6Jc_2_Reverse2_B04_04_ABI24.ab1
              1 CATCATCATT ACGCCAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC ACCATGGAAG
             61 ATGACCACAA GAACATCGTG CACGGCCCTG CCCCATTCTA CCCCCTGGAA GAGGGAACAG
            121 CCGGCGAGCA GCTGCACCGG GCCATGAAGA GATATGCCCA GGTGCCCGGC ACAATCGCCT
            181 TCACCGATGC CCACGTGGAA GTGAACATCA CCTACAGCGA GTACTTCGAG ATGGCCTGCC
            241 GGCTGGCCGA GACAATGAAG CGCTATGGCC TGGGCCTGCA GCACCACATT GCCGTGTGCA
            301 GCGAGAACAG CCTGCAGTTC TTCATGCCCG TGTGTGGCGC CCTGTTCATC GGAGTGGGAG
            361 TGGCCCCCAC CAACGACATC TACAACGAGA GAGAGCTGTA CAACAGCCTG AGCATCAGCC
            421 AGCCCACCAT CGTGTTCTGC AGCAAGCGGG CCCTGCAGAA AATCCTGGGC GTGCAGAAAA
            481 AGCTGCCCGT GATCGAGAAG ATCGTGATCC TGGACAGCCG CGAGGACTAC ATGGGCAAGC
            541 AGAGCATGTA CAGCTTCATC GAGAGCCATC TGCCCGCTGG CTTCAACGAG TACGACTACG
            601 TGCCCGACAC CTTCGACAGA GAGACAGCCA CCGCCCTGAT CATGAACAGC AGCGGCTCTA
            661 CCGGCCTGCC CAAGGGCGTG GAACTGACCC ACAAGAATGT GTGCGTGCGG TTCAGCCACT
            721 GCCGGGACCC TGTGTTCGGC AACCAGATCA TCCCCGACAC CGCTATCCTG ACCGTGATCC
            781 CCTTCCACCA CGGCTTCGGC ATGTTCACCA CCCTGGGCTA CCTGACCTGC GGCTTCCGGA
            841 TCGTGCTGAT GTACAGATTC GAGGAAGAAC TGTTCCTGCG GAGCCTGCAG GACTACAAGA
            901 TCCAGAGCGC CCTGCTGGTG CCTACCCTGT TCAGCTTCTT CGCCAAGAGC ACCCTGGTGG
            961 ATAAGTACGA CCTGAGCAAC CCTGCACGAG ATCGCCTCTG GGCGGAGCCC CCCCCTGGCT
           1021 AAGGAAGGTG GGAGAGCCCG TTGGTCAGCC GGATTCAAGC TGCCGTGGCA TCAGAACAGG
          1081 ACTTACGTCT TGACCGAAGC ACAACCCGTC G
          //
          Primer: pUC-M13_-100
          Sequence file: RDB14359_A6Jcb.seq check
          >D03707A1_A6Jc_2_pUC-M13_-100_A04_01_ABI24.ab1
              1 GGCCTGTCAG GCCGATTAGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC
             61 GACGGCCAGT GAATTCTCAC AGCTTGCTTT TCTTGCCCAT TGTCAGCATC TCGCGGATCT
            121 TCCGGCTGTC GATCTTGCCT GTCAGGCCCT TTGGCACCTC GTCCACGAAC TTCACGCCGC
            181 CTCTCAGCCT CTTGCTGGCG GTCACTTGTC CGGCCACGTA GTCCATCACT TCCTGCTCGG
            241 TCATGGTCTT GCCCTCTTCC AGCACCACCA CAGCGGCAGG CAGTTCGCCA GCATCAGCAT
            301 CAGGGATGCC GGCCACCCCG GCATCGAAGA TGAAGGGATG CTGCAGCAGG ATAGATTCCA
            361 GCTCGGCGGG TGGCACCTGG TAGCCCTGGT ACTTGATCAG GGACTTCAGC CGGTCCACGA
            421 TGAAGAAGTG GCCGTCCTTG TCGTAGTAGG CCAGGTCGCC AGAGTGCAGC CAGCCATCCT
            481 TGTCGATCAG GGCATTGGTG GCCTCGGGGT TGTTCACGTA GCCCTTCATA ATCATGGGGC
            541 CCTTCAGACA CAGCTCGCCC CTCTGGTTCA CGCCCAGTGT CTTGCTGGTG TCCAGGTCCA
            601 CAATCTTGGC GCTGAAAAAT GGGGCCACCT TTCCACAGGC GCCAGGCTTA TCGTCGCCCC
            661 TGGGGGTGAT GATCACGGCA GAGGTTGTCT CGGTCAGGCC GTAGCCCTGT CTGATGCCAG
            721 GCAGCTTGAA CCGCTTGGCC ACGGCCTCTC CCACTTCTTT AGCCAGGGGG GCTCCGCCAG
            781 AGGCGATCTC GTGCAGGTTG CTCAGGTCGT ACTTATCCAC CAGGGTGCTC TTGGCGAAGA
            841 AGCTGAACAG GGTAGGCACC AGCAGGGCGC TCTGGATCTT GTAGTCCTGC AGGCTCCGCA
            901 GGAACAGTTC TTCCTCGAAT CTGTACATCA GCACGATCCG GAAGCCGCAG TCAAGGTAGC
            961 CCAGGTGGTG AACATGCCGA AGCCGTGGGT GGAATGGGGA TCACGGGTCC AGGAATAGCG
           1021 GTGTCGGGGA TGATCTGGGA TTGCCGACAC ACGGTCCCGG CAGTGACCTG ATCGCACGCC
          1081 ACAACATTCT TTGTTGGGTT CAAGT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akiyoshi, R., Firefly luciferase. Japan Patent Abstracts 2012-235756.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl