Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA3.1(+)-Histac-H3K9/K14

For the detection of acetylation at the 9th and 14th lysine residues of histone H3 by the BRD4 bromodomain.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakaoka, S. et al., ACS Chem. Biol., 11 (3), 729-733, 2006)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakaoka, S. et al., ACS Chem. Biol., 11 (3), 729-733, 2006)。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14340_A6Icp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, insert 5' (mVenus 5') Sequence file: RDB14340_A6Ice.seq
>D03775A1_A6Ic_1_CMV-Forward_F08_06_ABI08.ab1     1 NNNNNNNNNN NNNNCNNTTG NNGCNNNGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT    61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC   121 GACTCACTAT AGGGAGACCC AAGCTGGCTA GCGTTTAAAC TTAAGCTTGG TACCATGGTG   181 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC   241 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG   301 CTGACCCTGA AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG   361 ACCACCCTGG GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT GAAGCAGCAC   421 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG   481 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC   541 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG   601 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCACCGCCG ACAAGCAGAA GAACGGCATC   661 AAGGCCAACT TCAAGATCCG CCACAACATC GAGGACGGCG GCGTGCAGCT CGCCGACNAC   721 TACCAGCAGA ACACCCCCAT CGNCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG   781 ANCTACCAGT CCGCCCTGAN CAAAGANCCC AACGAGAAGC GCGNTCACNT GGNCCTGCTG   841 NNNTNCGTGA NCGCCGCCNG GNTCNCTCTC GGNNTGNANN ANCTGTACNA GGAANTNTCC   901 NNNCCTANNN NGCCNNAGAN GNANANNNNN NNANTGNNNN ACNNGNTNNN ANNGNNNNTN 961 NNNNNNTANN GNAANNNNNN NNTNGNNTNN NNNTTNNNGC ANNNNNNNNN N //
Primer: BGH_rev2 (Pr0606) Region: bGH pA, insert 3' (ECFP 3') Sequence file: RDB14340_A6Icf.seq
>D03775A1_A6Ic_1_BGH_rev2_G08_07_ABI08.ab1     1 NNNNNNNNNN NGNNNNNNGG GGGNNGGGCN NACAACAGAN GGCTGGCAAC TAGAAGGCAC    61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTCTAGAC TCGAGTTACT TGTACAGCTC   121 GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG   181 CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG   241 CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT   301 GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTGGCCTTG ATGCCGTTCT TCTGCTTGTC   361 GGCGGTGATA TAGACGTTGT GGCTGATGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT   421 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC   481 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC   541 CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG   601 GTAGCGGCTG AAGCACTGCA CGCCCCAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC   661 GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTANG TGGNATCGCC   721 CTCGCCCTCG CCGGACACGC TGAACTTGTG GNCGTTTACG TCGCCGTCCA GCTCGACCAN   781 GNNGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ANCATGNTAT CNANCACTTT   841 GTACNAGAAA GCTGGGNCGA NAGCCCTCTN CCCGCNGNNG CNANGGGCCA GTTNGATGTC   901 CTTNGGNNTN NNGGNGANNC GCNNNGCGNN NNNNGNNNAC ANGNNNGNNN NNNCAANNNN 961 ANNNNNNNNN N //
Primer: hBRD4_N_R2 (Pr1042) Region: insert mid (hBRD4 5',mVenus 3') Sequence file: RDB14340_A6Icg.seq
>14340_14340_B1HuF_hBRD4_N_R2_E10_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNTT CTTNNTTGNT CCNNTATCCN TAGGCGTTTT AATGATCTTA    61 TAGTAATCAG GGAGGTTCAG CTTGACGGCA TCCACAGGCT GCTGGAAAGG CCATGCAAAC   121 TGGTGTTTCC ATAGTGTCTT GAGCACCACT CTGAGCAGGT ATTGCAGTTG GTTGGTCTGC   181 CTCTTGGGCT TGTTAGGGTT GGAGAATTCC TTGTACAGCT CGTCCATGCC GAGAGTGATC   241 CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG   301 CTCAGGGCGG ACTGGTAGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCG   361 ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC CGCCGTCCTC GATGTTGTGG   421 CGGATCTTGA AGTTGGCCTT GATGCCGTTC TTCTGCTTGT CGGCGGTGAT ATAGACGTTG   481 TGGCTGTTGT AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC CTTGAAGTCG   541 ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC CTCGGCGCGG   601 GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA GCCTTCGGGC   661 ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGGC GAAGCACTGC   721 AGGCCGTAGC CCAGGGTGGT CACGANGGTG GGCCAGGGCA CGGGCAGCTT GCCGGTGGTG   781 CAGATCAGCT TCAGGGTCAG CTTGCCGTAN GTGGCATCGC CCTCGCNCTC GCCGGACACG   841 CTGAACTTGT GGCCGTTTAC GTCGCCGTCC AGCTCGANCA GGATGGGCAC CACCCCGGTG   901 AACAGCNNCT CGCCCTTGCT CACCANGGTA CCNAGCTNNA GTTTAAACGC TAGCCNGCTT   961 GGNNTCTNCC TATANTGANN CGNANTNNTT NNNANAANNC NNGTAANCAN NNGGNNCNNN 1021 ANNTNNGNNN NNNNNCNNTG CTNANNTANA NNNNCNNNNN NNNNN //
Primer: mCerulean-N_R (Pr0770) Region: insert mid (ECFP 5',mHisH3,hBRD4 3') Sequence file: RDB14340_A6Ich.seq
>14340_14340_B1HuF_mCerulean-N_R_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGGGNAGC TTGCCGGTGG TGCAGATGAA CTTCAGGGTC    61 AGCTTGCCGT AGGTGGCATC GCCCTCGCCC TCGCCGGACA CGCTGAACTT GTGGCCGTTT   121 ACGTCGCCGT CCAGCTCGAC CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG   181 CTCACCATGA TATCAACCAC TTTGTACAAG AAAGCTGGGT CGACAGCCCT CTCCCCGCGG   241 ATGCGACGGG CCAGTTGGAT GTCCTTGGGC ATGATGGTGA CACGCTTGGC GTGGATGGCG   301 CACAGGTTGG TGTCCTCAAA CAGACCCACA AGGTAGGCCT CACAGGCCTC CTGCAGAGCC   361 ATGACTGCCG AGCTCTGGAA GCGCAGGTCG GTCTTGAAGT CCTGCGCGAT CTCGCGCACC   421 AGGCGCTGGA ACGGCAGCTT GCGGATCAGC AGCTCGGTCG ACTTCTGGTA ACGCCGGATC   481 TCGCGCAGCG CCACGGTGCC GGGACGGTAG CGGTGAGGCT TCTTCACGCC GCCGGTAGCC   541 GGGGCGCTCT TGCGGGCGGC CTTGGTGGCC AGCTGCTTAC GCGGGGCCTT GCCACCGGTG   601 GACTTGCGAG CGGTCTGCTT AGTACGAGCC ATGGATCCAG AACCTCCTCC ACCAGATCCA   661 CCTCCTCCGT CGACCACCAC TGGCTCCTCA GGCTCGTCCG GCATCTTGGC AAAGCGCATT   721 TCGAACACAT CCTGGAGCTT GCGGGCCATG GCCACCACCT CATGGTCAGG ANGGTTGTAC   781 TTATAGCANT TGGAGAACAT CAATCGGACG TCAGCACCAA ACTCCTGANC ATCACGGTAC   841 TCACGGGCCT CCAGTTTAGA CTTGATTGTG CTCATGTCCA TGGGGTGCTT GATGATGTCN   901 CAGTAGTCGT GTANNNCCAG TGNCTCCNCG NNCACAGGCT TGTANNANNG NNNNNGCGTN   961 NNNGNGTGCT TCTNGNNNAA NNTCNNCTNN ANNANNNNNC TGCNNCNNTT GANCTGCTNC  1021 NANANNNNNC TGNNNNNNNC NGNNGNNGGN NNCNGCNGNN NNNNNGNNNN NNNNNTNNNN 1081 NNNNNN //
Primer: attB2-R1 (Pr0411) Region: insert mid (mHisH3,hBRD4 3') Sequence file: RDB14340_A6Ici.seq
>14340_14340_B1HuF_attB2-R1_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNGGCNNG TTGGNNGNCC TTGGGCATGA TGGTGACACG CTTGGCGTGG    61 ATGGCGCACA GGTTGGTGTC CTCAAACAGA CCCACAAGGT AGGCCTCACA GGCCTCCTGC   121 AGAGCCATGA CTGCCGAGCT CTGGAAGCGC AGGTCGGTCT TGAAGTCCTG CGCGATCTCG   181 CGCACCAGGC GCTGGAACGG CAGCTTGCGG ATCAGCAGCT CGGTCGACTT CTGGTAACGC   241 CGGATCTCGC GCAGCGCCAC GGTGCCGGGA CGGTAGCGGT GAGGCTTCTT CACGCCGCCG   301 GTAGCCGGGG CGCTCTTGCG GGCGGCCTTG GTGGCCAGCT GCTTACGCGG GGCCTTGCCA   361 CCGGTGGACT TGCGAGCGGT CTGCTTAGTA CGAGCCATGG ATCCAGAACC TCCTCCACCA   421 GATCCACCTC CTCCGTCGAC CACCACTGGC TCCTCAGGCT CGTCCGGCAT CTTGGCAAAG   481 CGCATTTCGA ACACATCCTG GAGCTTGCGG GCCATGGCCA CCACCTCATG GTCAGGAGGG   541 TTGTACTTAT AGCAGTTGGA GAACATCAAT CGGACGTCAG CACCAAACTC CTGAGCATCA   601 CGGTACTCAC GGGCCTCCAG TTTAGACTTG ATTGTGCTCA TGTCCATGGG GTGCTTGATG   661 ATGTCACAGT AGTCGTGTAG GCCCAGTGCC TCCACGTCCA CAGGCTTGTA GAAGGGCCAG   721 GCGTAGGCGG CGTGCTTCTT GGCAAACATC TCCTTGAGGA TGCCGCTGCA GCACTTGAGC   781 TGCTCCGAGA NCTTGCTGCT CTTCTCTGGT GCTGGGTGCT GCTGAGAGTC NNGCACGTCC   841 TTCTTTGGAN GTTTCACAGG CCGGCTGCTC TCCNGCCGCT GGNCCANCTT GGTGGTCTTG   901 GGCTNCGGGG GCAGCNAGGG TGGCTCGNGN ANTGGGGTCA ATGGTGGNGG GGGGTGGNGG   961 TGNCNGNTTT CCTCTTCNNN NCNNNNNNNT TGNNNNNNNN NGNTGTGGGG NNGNNNGNNN 1021 ANGNNGGGNN NGGGNNNNNN NNNN //
Primer: SV40pro_F (Pr0110) Region: SV40 pro_ori, NeoR Sequence file: RDB14340_A6Icj.seq
>D03775A1_A6Ic_1_SV40pro-F_H06_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNGTGA GGNNGNTTTT TTGGAGGCCT AGGCTTTTGC    61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA   121 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG   181 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC   241 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG   301 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC   361 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG   421 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT   481 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG   541 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT   601 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC   661 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG   721 GTGGAAAATG NNCGCTTTTC TGGANTCATC GACTGTGGNC GGCTGGGTGT GGCGGACCGC   781 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAN AGCTTGGCGG CGAATGGGCT   841 GANCGCTTCC TCGNGCTTTA CGGNATCNCC GCTCCCGATN CGCAGCGCAT CGCCTTCTAT   901 CGCCTNCTTG ANNAGTTCTT CTNANCGGGN NTCTNGGGTT CGAAATGNNC GANCNANCNN   961 CGCCCNANNT GNCATCACNA GNNTNCNNNN NCNNNGCCNN NNNCNATNNA NNNNNNGGCN 1021 NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

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