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pcDNA3.1(+)-Histac-H3K9/K14

For the detection of acetylation at the 9th and 14th lysine residues of histone H3 by the BRD4 bromodomain.

Catalog number RDB14340
Resource name pcDNA3.1(+)-Histac-H3K9/K14
Clone info. Expression vector of Histac-H3K9/K14, a fluorescent probe to visualize acetylation of histone H3 Lys9 (H3K9) and histone H3 Lys14 (H3K14) in living cells by means of fluorescence resonance energy transfer (FRET), under the control of CMV promotor. See Fig. 1A of Nakaoka, S. et. al., ACS Chem. Biol. 11 (3): 729-733, 2016.
Comment Expression was confirmed by observation fluorescent in COS7 cells under the microscope by the depositor.
Vector backbone pcDNA3.1 (Plasmid)
Size of vector backbone 5.4 kb
Gene/insert name Human BRD4 cDNA Mouse Histone 3/n cDNA
Depositor|Developer Sasaki, Kazuki |
Other clones in our bank

External Database
Human BRD4 Mouse Histone 3/n

        Reference sequence
          
         
        Sequence (partial) RDB14340pka02.seq provided by the depositor

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakaoka, S. et al., ACS Chem. Biol., 11 (3), 729-733, 2006)
        Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding Venus. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        Approval form [Word] by Dr. Atsushi Miyawaki
        Remarks
        Please obtain written approval of Dr. Atsushi Miyawaki.
        提供案内 (日本国内) [open/close]

        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakaoka, S. et al., ACS Chem. Biol., 11 (3), 729-733, 2006)。
        付加的提供条件 利用者は、Venusについて理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。非営利機関の非営利学術研究に限って提供する。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
        宮脇敦史博士による提供承諾書 [Word]
        備考
        宮脇敦史博士の書面による提供承諾が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB14340 pcDNA3.1(+)-Histac-H3K9/K14 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Original reference

        original Nakaoka, S., A Genetically Encoded FRET Probe to Detect Intranucleosomal Histone H3K9 or H3K14 Acetylation Using BRD4, a BET Family Member. ACS Chem. Biol. 11 (3): 729-733 (2016). PMID 25946208.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Epigenetics clone (English text)
        Featured content Histac for visualization of acetylation of the Histon (English text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB14340_A6Icp1-2.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV_Forward
        Region: CMV pro, insert 5' (mVenus 5')
        Sequence file: RDB14340_A6Ice.seq check
        >D03775A1_A6Ic_1_CMV-Forward_F08_06_ABI08.ab1
            1 NNNNNNNNNN NNNNCNNTTG NNGCNNNGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
           61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
          121 GACTCACTAT AGGGAGACCC AAGCTGGCTA GCGTTTAAAC TTAAGCTTGG TACCATGGTG
          181 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
          241 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG
          301 CTGACCCTGA AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
          361 ACCACCCTGG GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT GAAGCAGCAC
          421 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG
          481 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
          541 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG
          601 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCACCGCCG ACAAGCAGAA GAACGGCATC
          661 AAGGCCAACT TCAAGATCCG CCACAACATC GAGGACGGCG GCGTGCAGCT CGCCGACNAC
          721 TACCAGCAGA ACACCCCCAT CGNCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
          781 ANCTACCAGT CCGCCCTGAN CAAAGANCCC AACGAGAAGC GCGNTCACNT GGNCCTGCTG
          841 NNNTNCGTGA NCGCCGCCNG GNTCNCTCTC GGNNTGNANN ANCTGTACNA GGAANTNTCC
          901 NNNCCTANNN NGCCNNAGAN GNANANNNNN NNANTGNNNN ACNNGNTNNN ANNGNNNNTN
        961 NNNNNNTANN GNAANNNNNN NNTNGNNTNN NNNTTNNNGC ANNNNNNNNN N
        //
        Primer: BGH_rev2
        Region: bGH pA, insert 3' (ECFP 3')
        Sequence file: RDB14340_A6Icf.seq check
        >D03775A1_A6Ic_1_BGH_rev2_G08_07_ABI08.ab1
            1 NNNNNNNNNN NGNNNNNNGG GGGNNGGGCN NACAACAGAN GGCTGGCAAC TAGAAGGCAC
           61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTCTAGAC TCGAGTTACT TGTACAGCTC
          121 GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG
          181 CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG
          241 CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT
          301 GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTGGCCTTG ATGCCGTTCT TCTGCTTGTC
          361 GGCGGTGATA TAGACGTTGT GGCTGATGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT
          421 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC
          481 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC
          541 CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG
          601 GTAGCGGCTG AAGCACTGCA CGCCCCAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC
          661 GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTANG TGGNATCGCC
          721 CTCGCCCTCG CCGGACACGC TGAACTTGTG GNCGTTTACG TCGCCGTCCA GCTCGACCAN
          781 GNNGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ANCATGNTAT CNANCACTTT
          841 GTACNAGAAA GCTGGGNCGA NAGCCCTCTN CCCGCNGNNG CNANGGGCCA GTTNGATGTC
          901 CTTNGGNNTN NNGGNGANNC GCNNNGCGNN NNNNGNNNAC ANGNNNGNNN NNNCAANNNN
        961 ANNNNNNNNN N
        //
        Primer: hBRD4_N_R2
        Region: insert mid (hBRD4 5',mVenus 3')
        Sequence file: RDB14340_A6Icg.seq check
        >14340_14340_B1HuF_hBRD4_N_R2_E10_05_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNTT CTTNNTTGNT CCNNTATCCN TAGGCGTTTT AATGATCTTA
           61 TAGTAATCAG GGAGGTTCAG CTTGACGGCA TCCACAGGCT GCTGGAAAGG CCATGCAAAC
          121 TGGTGTTTCC ATAGTGTCTT GAGCACCACT CTGAGCAGGT ATTGCAGTTG GTTGGTCTGC
          181 CTCTTGGGCT TGTTAGGGTT GGAGAATTCC TTGTACAGCT CGTCCATGCC GAGAGTGATC
          241 CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG
          301 CTCAGGGCGG ACTGGTAGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCG
          361 ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC CGCCGTCCTC GATGTTGTGG
          421 CGGATCTTGA AGTTGGCCTT GATGCCGTTC TTCTGCTTGT CGGCGGTGAT ATAGACGTTG
          481 TGGCTGTTGT AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC CTTGAAGTCG
          541 ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC CTCGGCGCGG
          601 GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA GCCTTCGGGC
          661 ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGGC GAAGCACTGC
          721 AGGCCGTAGC CCAGGGTGGT CACGANGGTG GGCCAGGGCA CGGGCAGCTT GCCGGTGGTG
          781 CAGATCAGCT TCAGGGTCAG CTTGCCGTAN GTGGCATCGC CCTCGCNCTC GCCGGACACG
          841 CTGAACTTGT GGCCGTTTAC GTCGCCGTCC AGCTCGANCA GGATGGGCAC CACCCCGGTG
          901 AACAGCNNCT CGCCCTTGCT CACCANGGTA CCNAGCTNNA GTTTAAACGC TAGCCNGCTT
          961 GGNNTCTNCC TATANTGANN CGNANTNNTT NNNANAANNC NNGTAANCAN NNGGNNCNNN
        1021 ANNTNNGNNN NNNNNCNNTG CTNANNTANA NNNNCNNNNN NNNNN
        //
        Primer: mCerulean-N_R
        Region: insert mid (ECFP 5',mHisH3,hBRD4 3')
        Sequence file: RDB14340_A6Ich.seq check
        >14340_14340_B1HuF_mCerulean-N_R_G01_07_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNGGGNAGC TTGCCGGTGG TGCAGATGAA CTTCAGGGTC
           61 AGCTTGCCGT AGGTGGCATC GCCCTCGCCC TCGCCGGACA CGCTGAACTT GTGGCCGTTT
          121 ACGTCGCCGT CCAGCTCGAC CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG
          181 CTCACCATGA TATCAACCAC TTTGTACAAG AAAGCTGGGT CGACAGCCCT CTCCCCGCGG
          241 ATGCGACGGG CCAGTTGGAT GTCCTTGGGC ATGATGGTGA CACGCTTGGC GTGGATGGCG
          301 CACAGGTTGG TGTCCTCAAA CAGACCCACA AGGTAGGCCT CACAGGCCTC CTGCAGAGCC
          361 ATGACTGCCG AGCTCTGGAA GCGCAGGTCG GTCTTGAAGT CCTGCGCGAT CTCGCGCACC
          421 AGGCGCTGGA ACGGCAGCTT GCGGATCAGC AGCTCGGTCG ACTTCTGGTA ACGCCGGATC
          481 TCGCGCAGCG CCACGGTGCC GGGACGGTAG CGGTGAGGCT TCTTCACGCC GCCGGTAGCC
          541 GGGGCGCTCT TGCGGGCGGC CTTGGTGGCC AGCTGCTTAC GCGGGGCCTT GCCACCGGTG
          601 GACTTGCGAG CGGTCTGCTT AGTACGAGCC ATGGATCCAG AACCTCCTCC ACCAGATCCA
          661 CCTCCTCCGT CGACCACCAC TGGCTCCTCA GGCTCGTCCG GCATCTTGGC AAAGCGCATT
          721 TCGAACACAT CCTGGAGCTT GCGGGCCATG GCCACCACCT CATGGTCAGG ANGGTTGTAC
          781 TTATAGCANT TGGAGAACAT CAATCGGACG TCAGCACCAA ACTCCTGANC ATCACGGTAC
          841 TCACGGGCCT CCAGTTTAGA CTTGATTGTG CTCATGTCCA TGGGGTGCTT GATGATGTCN
          901 CAGTAGTCGT GTANNNCCAG TGNCTCCNCG NNCACAGGCT TGTANNANNG NNNNNGCGTN
          961 NNNGNGTGCT TCTNGNNNAA NNTCNNCTNN ANNANNNNNC TGCNNCNNTT GANCTGCTNC
         1021 NANANNNNNC TGNNNNNNNC NGNNGNNGGN NNCNGCNGNN NNNNNGNNNN NNNNNTNNNN
        1081 NNNNNN
        //
        Primer: attB2-R1
        Region: insert mid (mHisH3,hBRD4 3')
        Sequence file: RDB14340_A6Ici.seq check
        >14340_14340_B1HuF_attB2-R1_F01_06_ABI08.ab1
            1 NNNNNNNNNN NNNNGGCNNG TTGGNNGNCC TTGGGCATGA TGGTGACACG CTTGGCGTGG
           61 ATGGCGCACA GGTTGGTGTC CTCAAACAGA CCCACAAGGT AGGCCTCACA GGCCTCCTGC
          121 AGAGCCATGA CTGCCGAGCT CTGGAAGCGC AGGTCGGTCT TGAAGTCCTG CGCGATCTCG
          181 CGCACCAGGC GCTGGAACGG CAGCTTGCGG ATCAGCAGCT CGGTCGACTT CTGGTAACGC
          241 CGGATCTCGC GCAGCGCCAC GGTGCCGGGA CGGTAGCGGT GAGGCTTCTT CACGCCGCCG
          301 GTAGCCGGGG CGCTCTTGCG GGCGGCCTTG GTGGCCAGCT GCTTACGCGG GGCCTTGCCA
          361 CCGGTGGACT TGCGAGCGGT CTGCTTAGTA CGAGCCATGG ATCCAGAACC TCCTCCACCA
          421 GATCCACCTC CTCCGTCGAC CACCACTGGC TCCTCAGGCT CGTCCGGCAT CTTGGCAAAG
          481 CGCATTTCGA ACACATCCTG GAGCTTGCGG GCCATGGCCA CCACCTCATG GTCAGGAGGG
          541 TTGTACTTAT AGCAGTTGGA GAACATCAAT CGGACGTCAG CACCAAACTC CTGAGCATCA
          601 CGGTACTCAC GGGCCTCCAG TTTAGACTTG ATTGTGCTCA TGTCCATGGG GTGCTTGATG
          661 ATGTCACAGT AGTCGTGTAG GCCCAGTGCC TCCACGTCCA CAGGCTTGTA GAAGGGCCAG
          721 GCGTAGGCGG CGTGCTTCTT GGCAAACATC TCCTTGAGGA TGCCGCTGCA GCACTTGAGC
          781 TGCTCCGAGA NCTTGCTGCT CTTCTCTGGT GCTGGGTGCT GCTGAGAGTC NNGCACGTCC
          841 TTCTTTGGAN GTTTCACAGG CCGGCTGCTC TCCNGCCGCT GGNCCANCTT GGTGGTCTTG
          901 GGCTNCGGGG GCAGCNAGGG TGGCTCGNGN ANTGGGGTCA ATGGTGGNGG GGGGTGGNGG
          961 TGNCNGNTTT CCTCTTCNNN NCNNNNNNNT TGNNNNNNNN NGNTGTGGGG NNGNNNGNNN
        1021 ANGNNGGGNN NGGGNNNNNN NNNN
        //
        Primer: SV40pro_F
        Region: SV40 pro_ori, NeoR
        Sequence file: RDB14340_A6Icj.seq check
        >D03775A1_A6Ic_1_SV40pro-F_H06_08_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNGTGA GGNNGNTTTT TTGGAGGCCT AGGCTTTTGC
           61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA
          121 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
          181 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
          241 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
          301 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
          361 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
          421 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
          481 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
          541 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
          601 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC
          661 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
          721 GTGGAAAATG NNCGCTTTTC TGGANTCATC GACTGTGGNC GGCTGGGTGT GGCGGACCGC
          781 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAN AGCTTGGCGG CGAATGGGCT
          841 GANCGCTTCC TCGNGCTTTA CGGNATCNCC GCTCCCGATN CGCAGCGCAT CGCCTTCTAT
          901 CGCCTNCTTG ANNAGTTCTT CTNANCGGGN NTCTNGGGTT CGAAATGNNC GANCNANCNN
          961 CGCCCNANNT GNCATCACNA GNNTNCNNNN NCNNNGCCNN NNNCNATNNA NNNNNNGGCN
        1021 NNNNNNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Nakaoka, S., A Genetically Encoded FRET Probe to Detect Intranucleosomal Histone H3K9 or H3K14 Acetylation Using BRD4, a BET Family Member. ACS Chem. Biol. 11 (3): 729-733 (2016). PMID 25946208.
        reference He, M., Chemical Biology Approaches for Investigating the Functions of Lysine Acetyltransferases. Angew. Chem. Int. Ed. Engl. 57 (5): 1162-1184 (2018). PMID 28786225.
        reference Soleja, N., Role of green fluorescent proteins and their variants in development of FRET-based sensors. J. Biosci. 43 (4): 763-784 (2018). PMID 30207321.

        2022.07.27

        GNP_filter3_RDBDEP_html_220727.pl