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pCXN2-FLAG-hMAVS (#RDB14329)

Expression vector of FLAG tagged human MAVS.

Clone info. Expression vector driven by the CAG promoter for FLAG tagged human mitochondrial antiviral-signaling protein (MAVS).
Comment Expression was confirmed by the deposotor with luciferase assay.
Vector backbone pCXN2-Flag (Plasmid)
Selectable markers Amp^r
Gene/insert name human MAVS cDNA
Depositor|Developer Takaoka, Akinori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yamada, T. et al., Nat. Immunol. 17 (6): 687-694, 2016)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14329 pCXN2-FLAG-hMAVS DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCXN2-FLAG-hMAVS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14329).

Reference section:

Yamada, T., Horimoto, H., Kameyama, T., Hayakawa, S., Yamato, H., Dazai, M., Takada, A., Kida, H., Bott, D., Zhou, A.C., Hutin, D., Watts, T.H., Asaka, M., Matthews, J., Takaoka, A., Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17 (6): 687-694 (2016). PMID 27089381. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14329_A6I5p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB14329_A6I5a.seq check
>D03656A7_A6I5_1_pAxCA_F1_A03_03_ABI24.ab1
    1 GAAAGGGATC TGCTCATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC
   61 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTCAA GCTTAGCCAC CATGGACTAC
  121 AAAGACGATG ACGATAAAGC AAGGCTCGAG GTCGACGGTA TCGATAAGCT TGATATCGAA
  181 TTCCCAATAC TAGCAATGCC GTTTGCTGAA GACAAGACCT ATAAGTATAT CTGCCGCAAT
  241 TTCAGCAATT TTTGCAATGT GGATGTTGTA GAGATTCTGC CTTACCTGCC CTGCCTCACA
  301 GCAAGAGACC AGGATCGACT GCGGGCCACC TGCACACTCT CAGGGAACCG GGACACCCTC
  361 TGGCATCTCT TCAATACCCT TCAGCGGCGG CCCGGCTGGG TGGAGTACTT CATTGCGGCA
  421 CTGAGGGGCT GTGAGCTAGT TGATCTCGCG GACGAAGTGG CCTCTGTCTA CCAGAGCTAC
  481 CAGCCTCGGA CCTCGGACCG TCCCCCAGAC CCACTGGAGC CACCGTCACT TCCTGCTGAG
  541 AGGCCAGGGC CCCCCACACC TGCTGCGGCC CACAGCATCC CCTACAACAG CTGCAGAGAG
  601 AAGGAGCCAA GTTACCCCAT GCCTGTCCAG GAGACCCAGG CGCCAGAGTC CCCAGGAGAG
  661 AATTCAGAGC AAGCCCTGCA GACGCTCAGC CCCAGAGCCA TCCCAAGGAA TCCAGATGGT
  721 GGCCCCCTGG AGTCCTCCTC TGACCTGGCA GCCCTCAGCC CTCTGACCTC CAGCGGGCAT
  781 CAGGAGCAGG ACACAGAACT GGGCAGTACC CACACAGCAG GTGCGACCTC CAGCCTCACA
  841 CCATCCCGTG GGCCTGTGTC TCCATCTGTC TCCTTCCAGC CCCTGGCCCG TTCCACCCCC
  901 AGGGCAAGCC GCTTGCCTGG ACCCACAGGG TCAGTTGTAT CTACTGGCAC CTCCTTCTCC
  961 TCCTCATCCC CTGGCTTGGC CCTCTGCAGG GGCTGCAGAG GGTAACAAGG GTGCAGAGAG
 1021 TGACCAGGGC CGAGCCCTAT CATCTGCTCA GTGGGGGCAG AGGCACTGCC CAACTCTCTG
1081 CCCCTTCGAA GTGCTACACT TGATGCCTGT TGAACACACA GTTGGCCCTG TGAAAAGT
//
Primer: pAxCA_R1
Sequence file: RDB14329_A6I5b.seq check
>D03656A7_A6I5_1_pAxCA_R1_B03_06_ABI24.ab1
    1 TTAACAGGCA TTGGCCACAC CAGCCACCAC CTTCTGATAG GCAGCCTGCA CCTGAGGAGT
   61 GAATTAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCGCG
  121 CAATTAACCC TCACTAAAGG GAACAAAAGC TGGAGCTCCA CCGCGGTGGC GGCCGCTCTA
  181 GAACTAGTGG ATCCCCCGGG CTGCAGGAAT TCCCAATACT AAGCCCAGGG CTTCACTAGT
  241 GCAGACGCCG CCGGTACAGC ACCACCAGGA GTGTGACTAC CAGCACCCCT GTCACAGCCA
  301 CCTGGAGCCA CAGAGCCCCA GGTGAGGGCC TGTGGCATGG CACCTCCCTC TCCTGGAACT
  361 TCCGGTCGGC TTGTGGCCTG GGGCCGCCAT CCGGGTCCGC AGGTGGCCCA GGGTTGCCCT
  421 CCAGGAGCTG GATGCTGGGG TTCTCAGCCA CGTGGATCCC AAAGGTGCCC TCGGACTTAT
  481 ACTCATTCTC CTCTGGGCCA TGGCAGGGCC CCATGCCCAA GGAGGTGCTG GCACTGATGG
  541 CAAGATCCTC GAAGCAGCCC GAGAACGGGC TGTCTACCTG GGATGCCAGC ACGCCAGGCT
  601 TACTCAGCTC CGACCCAAGG CCCCTATTCT CAGAGCTGCT GTCTAGCCAG GCTGAGCTGC
  661 CTCCAGTGGC GCCGGCGGGT GTTGGAGCTG CTGGGGTCTC CTCATTTCTG CTGCTCCCGT
  721 CAGTGGGGAC TGTGCTGGCA GACACCTTGG TGAGCACCAT GCTAGTAGGC ACTTTGGATG
  781 GCACCATGCC AGCACGGGTT GAGTTGATGG GCAATTTGGA TGGTGCTGGA TTGGTGAGCG
  841 CATTAGAAGG CACTGCACCA GGGGGCTTTG AGCTAGTTTG GCAACTTGGA GGGCACTGTG
  901 CTGACAGATG CTGGGTTGGC AGGCACTTTC AGGCCACTGT GTTCACAGCA TCAAGTGTAG
  961 CACTTGAGGC AGAGAGTGCA GTGCCTCTGC CCCACTGAGC AATGATAGCT CGGCTGCTCA
 1021 CTCTCTGCAC CTGTTACCTC TGCAGCCTTG CAAGTCAGCA GGATGAGAGG GAAGAGGTCA
1081 GTTATACACT GAA
//
Primer: Amp_R
Sequence file: RDB14329_A6I5c.seq check
>D03656A7_A6I5_1_Amp_R_E03_15_ABI24.ab1
    1 TTCGTTTATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
   61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGGGTCGAG CAGTGTGGTT TTCAAGAGGA AGCAAAAAGC CTCTCCACCC AGGCCTGGAA
  181 TGTTTCCACC CAATGTCGAG CAGTGTGGTT TTGCAAGAGG AAGCAAAAAG CCTCTCCACC
  241 CAGGCCTGGA ATGTTTCCAC CCAATGTCGA GCAAACCCCG CCCAGCGTCT TGTCATTGGC
  301 GAATTAATTC GAACACGCAG ATGCAGTCGG GGCGGCGCGG TCCCAGGTCC ACTTCGCATA
  361 TTAAGGTGAC GCGTGTGGCC TCGAACACCG AGCGACCCTG CAGCCAATAT GGGATCGGCC
  421 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC
  481 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG
  541 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG
  601 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC
  661 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT
  721 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG
  781 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC
  841 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG
  901 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC
  961 GATGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGG
 1021 CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG CCGACGCTAT CAAGACATAG
1081 CGATTGCTAA CCGTTGATAC TGCTGGAAGA GCTTGACGGC GAATGGGGCC TGGAACG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yamada, T., Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17 (6): 687-694 (2016). PMID 27089381. [link to RRC of NBRP]