Expression vector of V5-tagged human NECAB3 gene mutant.
Clone info. | Expression vector of V5-tagged human NECAB3 gene mutant; in which the catalytic His324 in the monooxygenase (ABM) domain is replaced with Ala, see Fig. 5A for details. |
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Comment | Expression was confirmed by the depositor with immunoprecipitation and real-time PCR. |
Vector backbone | pcDNA3 derivative (Plasmid) |
Selectable markers | Amp^r (E. coli), Neo^r (mammalian cells). |
Gene/insert name | human NECAB3 cDNA |
Depositor|Developer | Sakamoto, Takeharu | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakaoka, H.J. et al., Sci. Rep. 6: 22784, 2016) |
Remarks | Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nakaoka, H.J. et al., Sci. Rep. 6: 22784, 2016)。 |
備考 | Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB14312 | pcDNA3.1_V5-NECAB3_H/A | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The pcDNA3.1_V5-NECAB3_H/A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14312). |
Reference section:
Nakaoka, H.J., Hara, T., Yoshino, S., Kanamori, A., Matsui, Y., Shimamura, T., Sato, H., Murakami, Y., Seiki, M., Sakamoto, T., NECAB3 Promotes Activation of Hypoxia-inducible factor-1 during Normoxia and Enhances Tumourigenicity of Cancer Cells. Sci. Rep, 6: 22784 (2016). PMID 26948053. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Nakaoka, H.J., NECAB3 Promotes Activation of Hypoxia-inducible factor-1 during Normoxia and Enhances Tumourigenicity of Cancer Cells. Sci. Rep, 6: 22784 (2016). PMID 26948053. [link to RRC of NBRP] |
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