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pMXs-Puro-Flag-mouse CNOT3 (245-end) (#RDB14291)

Shuttle vector to generate retrovirus vector expressing FLAG-tagged mouse CNOT3 mutant.

Clone info. Shuttle vector to generate retrovirus vector expressing FLAG-tagged mouse CNOT3 mutant. This mutant was generated by deletion of the coiled-coil domain (1-244 AA), see Fig. 3a of Suzuki, T. Sci. Rep, 5: 14779, 2015.
Comment No gap between FLAG sequence and CNOT3 cDNA. Expression was confirmed by the depositor with immunoblot.
Vector backbone pMXs-puro (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r (E. coli), Pur^r (mammalian cells).
Gene/insert name mouse CNOT3 cDNA
Depositor|Developer Suzuki, Toru |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Suzuki, T. et al., Sci. Rep. 5: 14779, 2015)
Additional terms and conditions:
Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Suzuki, T. et al., Sci. Rep. 5: 14779, 2015)。
MTAに書く付加的使用条件：
pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14291 pMXs-Puro-Flag-mouse CNOT3 (245-end) Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pMXs-Puro-Flag-mouse CNOT3 (245-end) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14291).

Reference section:

Suzuki, T., Kikuguchi, C., Sharma, S., Sasaki, T., Tokumasu, M., Adachi, S., Natsume, T., Kanegae, Y., Yamamoto, T., CNOT3 suppression promotes necroptosis by stabilizing mRNAs for cell death-inducing proteins. Sci. Rep. 5: 14779 (2015). PMID 26437789. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

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References

Original, user report and related articles

original Suzuki, T., CNOT3 suppression promotes necroptosis by stabilizing mRNAs for cell death-inducing proteins. Sci. Rep. 5: 14779 (2015). PMID 26437789. [link to RRC of NBRP]