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psiCHECK1-CXCR4-MM4 (#RDB14244)

Expression vector of shRNA of 4x CXCR4 fragment with renilla luciferase gene, missmatch.

Clone info. Expression vector of shRNA of 4x CXCR4 fragment with renilla luciferase gene, missmatch.
Comment Expression was confirmed by the depositor with RNAi assay.
Vector backbone psiCHECK-1 (Plasmid)
Size of vector backbone 3.6 kb
Selectable markers Amp^r
Gene/insert name human CXCR4 cDNA
Depositor|Developer Ui-Tei, Kumiko |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., Nucleic Acids Res. 43 (20): 9856-9873, 2015). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishi, K. et al., Nucleic Acids Res. 43(20):9856-9873, 2015)。教育研究目的での使用に限る。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14244 psiCHECK1-CXCR4-MM4 Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The psiCHECK1-CXCR4-MM4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14244).

Reference section:

Nishi, K., Takahashi, T., Suzawa, M., Miyakawa, T., Nagasawa, T., Ming, Y., Tanokura, M., Ui-Tei, K., Control of the localization and function of a miRNA silencing component TNRC6A by Argonaute protein. Nucleic Acids Res. 43 (20): 9856-9873 (2015). PMID 26446993. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Nishi, K., Control of the localization and function of a miRNA silencing component TNRC6A by Argonaute protein. Nucleic Acids Res. 43 (20): 9856-9873 (2015). PMID 26446993. [link to RRC of NBRP]