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pSUPER-retro-puro-DsRed

Retroviral packaging vector for shRNA of DsRed fragment.

Catalog number RDB14240
Resource name pSUPER-retro-puro-DsRed
Clone info. Retroviral Packaging Vector for shRNA of DsRed fragment.
Expression was confirmed by the depositor with RNAi assay.
Vector backbone pSUPER.retro.puro (Plasmid)
Size of vector backbone 7.3 kb
Selectable markers Amp^r (E. coli), Pur^r (mammalian cell).
Gene/insert name Discosoma sp red fluorescent protein (RFP) cDNA
Depositor Ui-Tei, Kumiko |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., Nucleic Acids Res. 43 (20): 9856-9873, 2015). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.
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Remarks Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14240 pSUPER-retro-puro-DsRed Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

2019.07.19

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