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pcDNA3.1-myc-GFP-TNRC6A-del.GW-I+II+III-NES-mut

Expression vector of myc-GFP-tagged human TNRC6A, NES-mutation and deletion of GW-I and II and GW-III.

Catalog number RDB14235
Resource name pcDNA3.1-myc-GFP-TNRC6A-del.GW-I+II+III-NES-mut
Clone info. Expression vector of myc-GFP-tagged human TNRC6A-NES-mutation (Fig. 1P), deletion of GW-I and II and GW-III (Fig. 1A of Nishi, K. et al. RNA 19 (1): 17-35, 2013).
Expression was confirmed by the depositor with microscopic observation and Western blotting.
Vector backbone pcDNA3.1-myc (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell).
Gene/insert name human TNRC6A cDNA Aquorea victoria green flurescent protein (GFP) cDNA
Depositor Ui-Tei, Kumiko |
 
Other clones in our bank human TNRC6A (NCBI Gene 27327) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.
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Schedule A (GFP-A) [Link]
Remarks
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14235 pcDNA3.1-myc-GFP-TNRC6A-del.GW-I+II+III-NES-mut Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

2019.07.19

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