Expression vector of myc-GFP-tagged human TNRC6A, NES-mutation and deletion of GW-I and II.
Clone info. | Expression vector of myc-GFP-tagged human TNRC6A-NES-mutation (Fig. 1P), deletion of GW-I and II (Fig. 1A of Nishi, K. et al. RNA 19 (1): 17-35, 2013). |
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Comment | Expression was confirmed by the depositor with microscopic observation and Western blotting. |
Vector backbone | pcDNA3.1-myc (Plasmid) |
Size of vector backbone | 5.5 kb |
Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell). |
Gene/insert name | human TNRC6A cDNA Aquorea victoria green flurescent protein (GFP) cDNA |
Depositor|Developer | Ui-Tei, Kumiko | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishi, K. et al., RNA, 19(1):17-35, 2013)。教育研究目的での使用に限る。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB14232 | pcDNA3.1-myc-GFP-TNRC6A-del.GW-I+II-NES-mut | Under QC test. Please contact us. | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pcDNA3.1-myc-GFP-TNRC6A-del.GW-I+II-NES-mut was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14232). |
Reference section:
Nishi, K., Nishi, A., Nagasawa, T., Ui-Tei, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. [link to RRC of NBRP] |
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