Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3.1-myc-GFP-TNRC6A-del.GW-I+II-NES-mut (#RDB14232)

Expression vector of myc-GFP-tagged human TNRC6A, NES-mutation and deletion of GW-I and II.

Clone info. Expression vector of myc-GFP-tagged human TNRC6A-NES-mutation (Fig. 1P), deletion of GW-I and II (Fig. 1A of Nishi, K. et al. RNA 19 (1): 17-35, 2013).
Comment Expression was confirmed by the depositor with microscopic observation and Western blotting.
Vector backbone pcDNA3.1-myc (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell).
Gene/insert name human TNRC6A cDNA Aquorea victoria green flurescent protein (GFP) cDNA
Depositor|Developer Ui-Tei, Kumiko |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishi, K. et al., RNA, 19(1):17-35, 2013)。教育研究目的での使用に限る。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14232 pcDNA3.1-myc-GFP-TNRC6A-del.GW-I+II-NES-mut Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-myc-GFP-TNRC6A-del.GW-I+II-NES-mut was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14232).

Reference section:

Nishi, K., Nishi, A., Nagasawa, T., Ui-Tei, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. [link to RRC of NBRP]