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pcDNA3.1-myc-GFP-TNRC6A-del.GW-II-NES-mut

Expression vector of myc-GFP-tagged human TNRC6A, NES-mutation and deletion of GW-II.

Catalog number RDB14231
Resource name pcDNA3.1-myc-GFP-TNRC6A-del.GW-II-NES-mut
Clone info. Expression vector of myc-GFP-tagged human TNRC6A-NES-mutation (Fig. 1P), deletion of GW-II (Fig. 1A of Nishi, K. et al. RNA 19 (1): 17-35, 2013).
Comment Expression was confirmed by the depositor with microscopic observation and Western blotting.
Vector backbone pcDNA3.1-myc (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell).
Gene/insert name human TNRC6A cDNA Aquorea victoria green flurescent protein (GFP) cDNA
Depositor|Developer Ui-Tei, Kumiko |
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External Database
human TNRC6A Aquorea victoria green flurescent protein (GFP)
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishi, K. et al., RNA, 19(1):17-35, 2013)。教育研究目的での使用に限る。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB14231 pcDNA3.1-myc-GFP-TNRC6A-del.GW-II-NES-mut Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

          2022.07.13

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