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pcDNA5-FLAG/HA/SBP-TNRC6A-NES/NLS-mut

Expression vector of FLAG/HA/SBP-tagged human TNRC6A, NLS and NES-mutation.

Catalog number RDB14222
Resource name pcDNA5-FLAG/HA/SBP-TNRC6A-NES/NLS-mut
Clone info. Expression vector of FLAG/HA/streptavidin binding peptide (SBP)-tagged human TNRC6A-NLS and NES-mutation (Fig. 1P, O of Nishi, K. et al. RNA 19 (1): 17-35, 2013).
Comment The peptide sequence of FLAG-tag is DYKDDDDDK instead of canonical sequence (DYKDDDDK). Expression was confirmed by the depositor with Western blotting.
Vector backbone pcDNA 5/FRT/TO (Plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r (E. coli), Hygro^r (mammalian cell).
Gene/insert name human TNRC6A cDNA
Depositor|Developer Ui-Tei, Kumiko |
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External Database
human TNRC6A
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishi, K. et al., RNA, 19(1):17-35, 2013)。教育研究目的での使用に限る。
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          RDB14222 pcDNA5-FLAG/HA/SBP-TNRC6A-NES/NLS-mut Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

          2022.05.17

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