Resource data sheet
Prev.

pcDNA5-FLAG/HA/SBP-TNRC6A-NES-mut

Expression vector of FLAG/HA/SBP-tagged human TNRC6A, NES-mutation.

Catalog number RDB14220
Resource name pcDNA5-FLAG/HA/SBP-TNRC6A-NES-mut
Clone info. Expression vector of FLAG/HA/streptavidin binding peptide (SBP)-tagged human TNRC6A-NES-mutation (Fig. 1P of Nishi, K. et al. RNA 19 (1): 17-35, 2013).
The peptide sequence of FLAG-tag is DYKDDDDDK instead of canonical sequence (DYKDDDDK). Expression was confirmed by the depositor with Western blotting.
Vector backbone pcDNA 5/FRT/TO (Plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r (E. coli), Hygro^r (mammalian cell).
Gene/insert name human TNRC6A cDNA
Depositor Ui-Tei, Kumiko |
 
Other clones in our bank human TNRC6A (NCBI Gene 27327) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.
Ordering Please visit Information of Request for Distribution. 
Order form [Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14220 pcDNA5-FLAG/HA/SBP-TNRC6A-NES-mut Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References

Original, user report and related articles

original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

2019.07.19

GNP_filter3_RDBDEP_html_190432.pl