Expression vector of FLAG/HA/SBP-tagged human TNRC6A, NES-mutation.
Catalog number | RDB14220 |
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Resource name | pcDNA5-FLAG/HA/SBP-TNRC6A-NES-mut |
Clone info. | Expression vector of FLAG/HA/streptavidin binding peptide (SBP)-tagged human TNRC6A-NES-mutation (Fig. 1P of Nishi, K. et al. RNA 19 (1): 17-35, 2013). The peptide sequence of FLAG-tag is DYKDDDDDK instead of canonical sequence (DYKDDDDK). Expression was confirmed by the depositor with Western blotting. |
Vector backbone | pcDNA 5/FRT/TO (Plasmid) |
Size of vector backbone | 5.1 kb |
Selectable markers | Amp^r (E. coli), Hygro^r (mammalian cell). |
Gene/insert name | human TNRC6A cDNA |
Depositor | Ui-Tei, Kumiko | |
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Other clones in our bank | human TNRC6A (NCBI Gene 27327) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only. |
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Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB14220 | pcDNA5-FLAG/HA/SBP-TNRC6A-NES-mut | Under QC test. Please contact us. | DNA solution |
Electronic file
Original reference
original | Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. |
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Further references such as user reports and related articles (go to bottom)
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This clone will be sequenced a portion and digested by restriction enzyme for examination.
Original, user report and related articles
original | Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. |
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2019.07.19
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