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pcDNA5-FLAG/HA/SBP-TNRC6A (#RDB14219)

Expression vector of FLAG/HA/SBP-tagged human TNRC6A.

Clone info. Expression vector of FLAG/HA/streptavidin binding peptide (SBP)-tagged human TNRC6A.
Comment The peptide sequence of FLAG-tag is DYKDDDDDK instead of canonical sequence (DYKDDDDK). Expression was confirmed by the depositor with Western blotting.
Vector backbone pcDNA 5/FRT/TO (Plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r (E. coli), Hygro^r (mammalian cell).
Gene/insert name human TNRC6A cDNA
Depositor|Developer Ui-Tei, Kumiko |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishi, K. et al., RNA, 19(1):17-35, 2013)。教育研究目的での使用に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14219 pcDNA5-FLAG/HA/SBP-TNRC6A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA5-FLAG/HA/SBP-TNRC6A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14219).

Reference section:

Nishi, K., Nishi, A., Nagasawa, T., Ui-Tei, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14219_A6Fup1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14219_A6Fua.seq check
>D03279A7_A6Fu_1_CMV-Forward_A10_01.ab1
    1 ATATCACTCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC CCTATCAGTG ATAGAGATCT CCCTATCAGT GATAGAGATC GTCGACGAGC
  121 TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG
  181 AAGACACCGG GACCGATCCA GCCTCCGGAC TCTAGCGTTT CCACCGCGGT GGCGGCCGCC
  241 ACCATGGATT ACAAGGATGA CGACGACGAT AAGTACCCAT ACGATGTTCC AGATTACGCT
  301 TCTATGGACG AGAAGACCAC CGGCTGGCGG GGCGGCCACG TGGTGGAGGG CCTGGCCGGC
  361 GAGCTGGAGC AGCTGCGGGC CAGGCTGGAG CACCACCCTC AGGGCCAGCG GGAGCCCTGC
  421 CCGGGCGGAT CCATGGATGC TGATTCTGCC TCCAGTTCTG AATCAGAGAG AAACATCACT
  481 ATCATGGCTT CAGGGAACAC AGGTGGTGAA AAAGATGGCC TTCGGAATAG CACTGGACTT
  541 GGTTCCCAAA ACAAGTTTGT AGTTGGTAGC AGCAGCAATA ATGTGGGCCA TGGAAGTAGT
  601 ACTGGGCCAT GGGGTTTTTC CCATGGAGCC ATAATAAGCA CATGTCAGGT CTCTGTGGAT
  661 GCTCCTGAAA GCAAATCTGA AAGTAGCAAC AATAGAATGA ATGCTTGGGG CACTGTAAGT
  721 TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC
  781 TGGCCAGTAT TAGAGAACAA TGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGTAGTTCT
  841 GGCATTAATA TTCAGTGCAG TACTATAGGC CAGATGCCTA ACAATCAGAG TATTAACTCT
  901 AAAGTGAGTG GTGGGTTCTA CCCATGGTAC CTGGGGAAAG CCCTTTCAGA AACTTTGTGA
  961 AATCTGAAGT AAGGTGGGTA CACAGAAGTT TCCAATTCCA GTTGATCCAA ACCTCAAAAT
1021 ATTAACTACT TGGAATTGGA CTGGATCGAA TAACACTTAC CTAACCTTTT TATTG
//
Primer: TNRC_NES-NLS-GW3
Sequence file: RDB14219_A6Fub.seq check
>D03279A7_A6Fu_1_TNRC6A_NES-NLS-GW3_B10_04.ab1
    1 ACTGAGGCTG GGCTGATGAT CTTGCATGGG AGCTTCATGC TTTGACTGGA CATAAACTGC
   61 ATGTTTTGGG ATTCATCTGC TACAATGCCA TCCTTATCAA AATAAGGATT GCGCTCCATG
  121 GAAGACTCTT TGGAAATCTG AGGCCGAGAA CCAGGGCCTT TCCCGACCGT TCGATTGTAA
  181 TCACCAATAT TTAGGCTATG TTTATCTATC TCCATTCGTT TGTCTTGTAA CATTCCTCCA
  241 GAAAGGTCCA TCTTATTGCT TTGTACATTT TCCTCTGGTG AGTCTCTCGA AAAACTGATG
  301 TTTGAAAACT GTTTAACAAA TGGATTTATC CACGCTTCTT CTTGTTTGTT TCCACCTTTC
  361 ATCATTCCCC TTTCCCTTCT CCTTTTTTTG CCACTCAGAC CCTTCGATGA CATTTTCTTT
  421 GTATATGGTG GCCAATTTAA AGATGAGTTA AGCTCTTGAG ATGAATTACT ATTCCACATT
  481 CCAATCTCCA CATCCTCTTC CTCTTCCCAG CCAGTGGGGC GATTTCCAGG CAATGGCATA
  541 TCATCACCAC ACCAGCCATC TTGCATAGAT TTTGGCCCAG ATTTGCTTAA TGCTTGTGGA
  601 CCAACAGAGC TGGAGCCCCA CGTGGATGTG CTGGATGCCG CAGCAATGGG TTCCCCCCAG
  661 CTGGGACCAC TGTCTATGGG CTTACCCCAT GCTGAAGTAC CATTATCCAC AGTTGTGGCT
  721 GGAGTAGAAG GCTCCCCCCA GGGCTCACCC CAGCCAGAGC CACTGCTTGC CTCTTTGTTT
  781 GAGATGGCAC TTGCAGGCGT TAGCAGCTGA TGTACCTGTG CTTGCTGGTC TGAACGGCTG
  841 TTGCCATTTG GGACGTTTTT TGTTCCACAT GTTCACATTT TTTGTAGTTG TATTTGCTTG
  901 GAATCTCCCC CAAGCTGAAG TTTCCATCAT CAATCTCCAA TTTTGCGACG TATAGATTCT
  961 GGGGGATGGG TTTCCCTCCC AGCCTGTGGT TTCATCCTTC TTTTGCTTGG GGCTGGGTAT
 1021 AGTCCCCCAG CAAGCCTGGT TACCAGAGGA TTTGCTGTAG CTGTTGGAAT CCCCCAAAGA
1081 AATCCA
//
Primer: BGH_rev2
Sequence file: RDB14219_A6Fuc.seq check
>D03279A7_A6Fu_1_BGH_rev2_C10_07.ab1
    1 CAATTGGATA AGTAGCACGG GGGAGGGGCA ACAACAGATG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGGGT TTCGAGTGAA TTGTAATACG ACTCACTATA GGGCGAATTG
  121 GGTACACTTA CCTGGTACCT TAATTAATTA AGGTACCGGG CCCCCCCTCG AGGTCGACGG
  181 TATCGATAAG CTTGATATCC TAGATTACAT GGACTCTCCA CCCCCACCCA GGTGGTCAAC
  241 AGAAAGAAAA GCGTTAATGG GAGATGGGCT GCTAATTCCT CGGGGGTCGC TGCTGCTTGG
  301 GGGACCCCAC AGGCTTGTGG AATAATGCGG GGTCCCCCAG AGTGAAGTGC CGTGAAGGTC
  361 TCCACAGTTA GTTCCCGACA GCCCAGCACC ATTCCAGTGA TTGAGATCGG TCCGGCTGGA
  421 GAATGAGTGG GAACAGTCGA GGGAGCCCAG CCGGCTCTGG CTGGACCCGA GAGACTGCCA
  481 GCCGGGAGAA GGGGTCAGAG ACTGGCTTTG TGCAAAGAAA CGACTGATCT CCTCTTCACT
  541 GGCAAACTCA GCAAGAATAG TAGTGTTCCC CAGTACACAC ATGTGCAGAG ACTTTTGTGC
  601 CTTCACTACC TCTTCTTTTG AACTGTAGCG GACCAGAGCA TTTCCGTGAG GGAGGTTCAG
  661 GTGGAATGTG ATCAGCGGGC CGTGCTGCAT GCACAGAGTG CGCAGAGTTG AGCCATCGAT
  721 CTGAGGTGTA AGGTTTTTTT AGAACAAGCC AATTTGTTAT TCTCCCTGAG CTGCTCTCAC
  781 CCCAGCTGGA ACCTGGGGGT ATATCTGGCG TCAGAATTTC CCCATCCCTC CACCTATACG
  841 AAAGGGGAGA ATTATCCCAC GTAGACAAGG GTGGCTTTCT GACCAGTCAG TCCCGGGAGG
  901 TGGGGCGGGA CGAGCAGTGA TGTTTTTTAG GTGGGCAAAG GACTTTCACA GCTCATGGAG
  961 CCAGAAGGAG GTGTTTGTAA CTGACCAGAG ACATGTCATT GGATCACTAT TTCTGCCTGG
1021 ACTTGCTAGT GCTGTACTGC TTGAGGGAAG CCGGATG
//
Primer: BGHpA_F
Sequence file: RDB14219_A6Fud.seq check
>D03279A7_A6Fu_1_BGHpA_F_D10_10.ab1
    1 CGGGATGGTT ATCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG
   61 AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA
  121 CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG
  181 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT
  241 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC
  301 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG
  361 ATTAGGGTGA TGGTTCACGT ACCTAGAAGT TCCTATTCCG AAGTTCCTAT TCTCTAGAAA
  421 GTATAGGAAC TTCCTTGGCC AAAAAGCCTG AACTCACCGC GACGTCTGTC GAGAAGTTTC
  481 TGATCGAAAA GTTCGACAGC GTCTCCGACC TGATGCAGCT CTCGGAGGGC GAAGAATCTC
  541 GTGCTTTCAG CTTCGATGTA GGAGGGCGTG GATATGTCCT GCGGGTAAAT AGCTGCGCCG
  601 ATGGTTTCTA CAAAGATCGT TATGTTTATC GGCACTTTGC ATCGGCCGCG CTCCCGATTC
  661 CGGAAGTGCT TGACATTGGG GAATTCAGCG AGAGCCTGAC CTATTGCATC TCCCGCCGTG
  721 CACAGGGTGT CACGTTGCAA GACCTGCCTG AAACCGAACT GCCCGCTGTT CTGCAGCCGG
  781 TCGCGGAGGC CATGGATGCG ATCGCTGCGG CCGATCTTAG CCAGACGAGC GGGTTCGGCC
  841 CATTCGGACC GCAAAGGAAT CGGTCAATAC ACTACATGGC GTGATTTCAT ATGCGCGATT
  901 GCTGATCCCC ATGTGTATCA CTGGCAAACT GTGATGGGAC GACACCGTCA GTGCGTCCGT
  961 CGCGCAGCTC TCGATGAGCT GATGCTTTGG GCCGAAGACT GCCCCGAAGG TCAGCACCTC
 1021 GTGCACGCGG AATTTCGCTC GACCATGTCT GACGGACATG ACGGCATAAC CAGCCGGGTT
1081 CAATTGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. [link to RRC of NBRP]