Expression vector of FLAG/HA/SBP-tagged human TNRC6A.
Clone info. | Expression vector of FLAG/HA/streptavidin binding peptide (SBP)-tagged human TNRC6A. |
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Comment | The peptide sequence of FLAG-tag is DYKDDDDDK instead of canonical sequence (DYKDDDDK). Expression was confirmed by the depositor with Western blotting. |
Vector backbone | pcDNA 5/FRT/TO (Plasmid) |
Size of vector backbone | 5.1 kb |
Selectable markers | Amp^r (E. coli), Hygro^r (mammalian cell). |
Gene/insert name | human TNRC6A cDNA |
Depositor|Developer | Ui-Tei, Kumiko | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishi, K. et al., RNA, 19(1):17-35, 2013)。教育研究目的での使用に限る。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB14219 | pcDNA5-FLAG/HA/SBP-TNRC6A | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pcDNA5-FLAG/HA/SBP-TNRC6A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14219). |
Reference section:
Nishi, K., Nishi, A., Nagasawa, T., Ui-Tei, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB14219_A6Fup1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV-Forward Sequence file: RDB14219_A6Fua.seq ![]() |
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>D03279A7_A6Fu_1_CMV-Forward_A10_01.ab1 1 ATATCACTCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTCTC CCTATCAGTG ATAGAGATCT CCCTATCAGT GATAGAGATC GTCGACGAGC 121 TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG 181 AAGACACCGG GACCGATCCA GCCTCCGGAC TCTAGCGTTT CCACCGCGGT GGCGGCCGCC 241 ACCATGGATT ACAAGGATGA CGACGACGAT AAGTACCCAT ACGATGTTCC AGATTACGCT 301 TCTATGGACG AGAAGACCAC CGGCTGGCGG GGCGGCCACG TGGTGGAGGG CCTGGCCGGC 361 GAGCTGGAGC AGCTGCGGGC CAGGCTGGAG CACCACCCTC AGGGCCAGCG GGAGCCCTGC 421 CCGGGCGGAT CCATGGATGC TGATTCTGCC TCCAGTTCTG AATCAGAGAG AAACATCACT 481 ATCATGGCTT CAGGGAACAC AGGTGGTGAA AAAGATGGCC TTCGGAATAG CACTGGACTT 541 GGTTCCCAAA ACAAGTTTGT AGTTGGTAGC AGCAGCAATA ATGTGGGCCA TGGAAGTAGT 601 ACTGGGCCAT GGGGTTTTTC CCATGGAGCC ATAATAAGCA CATGTCAGGT CTCTGTGGAT 661 GCTCCTGAAA GCAAATCTGA AAGTAGCAAC AATAGAATGA ATGCTTGGGG CACTGTAAGT 721 TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC 781 TGGCCAGTAT TAGAGAACAA TGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGTAGTTCT 841 GGCATTAATA TTCAGTGCAG TACTATAGGC CAGATGCCTA ACAATCAGAG TATTAACTCT 901 AAAGTGAGTG GTGGGTTCTA CCCATGGTAC CTGGGGAAAG CCCTTTCAGA AACTTTGTGA 961 AATCTGAAGT AAGGTGGGTA CACAGAAGTT TCCAATTCCA GTTGATCCAA ACCTCAAAAT 1021 ATTAACTACT TGGAATTGGA CTGGATCGAA TAACACTTAC CTAACCTTTT TATTG // |
Primer: TNRC_NES-NLS-GW3 Sequence file: RDB14219_A6Fub.seq ![]() |
>D03279A7_A6Fu_1_TNRC6A_NES-NLS-GW3_B10_04.ab1 1 ACTGAGGCTG GGCTGATGAT CTTGCATGGG AGCTTCATGC TTTGACTGGA CATAAACTGC 61 ATGTTTTGGG ATTCATCTGC TACAATGCCA TCCTTATCAA AATAAGGATT GCGCTCCATG 121 GAAGACTCTT TGGAAATCTG AGGCCGAGAA CCAGGGCCTT TCCCGACCGT TCGATTGTAA 181 TCACCAATAT TTAGGCTATG TTTATCTATC TCCATTCGTT TGTCTTGTAA CATTCCTCCA 241 GAAAGGTCCA TCTTATTGCT TTGTACATTT TCCTCTGGTG AGTCTCTCGA AAAACTGATG 301 TTTGAAAACT GTTTAACAAA TGGATTTATC CACGCTTCTT CTTGTTTGTT TCCACCTTTC 361 ATCATTCCCC TTTCCCTTCT CCTTTTTTTG CCACTCAGAC CCTTCGATGA CATTTTCTTT 421 GTATATGGTG GCCAATTTAA AGATGAGTTA AGCTCTTGAG ATGAATTACT ATTCCACATT 481 CCAATCTCCA CATCCTCTTC CTCTTCCCAG CCAGTGGGGC GATTTCCAGG CAATGGCATA 541 TCATCACCAC ACCAGCCATC TTGCATAGAT TTTGGCCCAG ATTTGCTTAA TGCTTGTGGA 601 CCAACAGAGC TGGAGCCCCA CGTGGATGTG CTGGATGCCG CAGCAATGGG TTCCCCCCAG 661 CTGGGACCAC TGTCTATGGG CTTACCCCAT GCTGAAGTAC CATTATCCAC AGTTGTGGCT 721 GGAGTAGAAG GCTCCCCCCA GGGCTCACCC CAGCCAGAGC CACTGCTTGC CTCTTTGTTT 781 GAGATGGCAC TTGCAGGCGT TAGCAGCTGA TGTACCTGTG CTTGCTGGTC TGAACGGCTG 841 TTGCCATTTG GGACGTTTTT TGTTCCACAT GTTCACATTT TTTGTAGTTG TATTTGCTTG 901 GAATCTCCCC CAAGCTGAAG TTTCCATCAT CAATCTCCAA TTTTGCGACG TATAGATTCT 961 GGGGGATGGG TTTCCCTCCC AGCCTGTGGT TTCATCCTTC TTTTGCTTGG GGCTGGGTAT 1021 AGTCCCCCAG CAAGCCTGGT TACCAGAGGA TTTGCTGTAG CTGTTGGAAT CCCCCAAAGA 1081 AATCCA // |
Primer: BGH_rev2 Sequence file: RDB14219_A6Fuc.seq ![]() |
>D03279A7_A6Fu_1_BGH_rev2_C10_07.ab1 1 CAATTGGATA AGTAGCACGG GGGAGGGGCA ACAACAGATG GCTGGCAACT AGAAGGCACA 61 GTCGAGGCTG ATCAGCGGGT TTCGAGTGAA TTGTAATACG ACTCACTATA GGGCGAATTG 121 GGTACACTTA CCTGGTACCT TAATTAATTA AGGTACCGGG CCCCCCCTCG AGGTCGACGG 181 TATCGATAAG CTTGATATCC TAGATTACAT GGACTCTCCA CCCCCACCCA GGTGGTCAAC 241 AGAAAGAAAA GCGTTAATGG GAGATGGGCT GCTAATTCCT CGGGGGTCGC TGCTGCTTGG 301 GGGACCCCAC AGGCTTGTGG AATAATGCGG GGTCCCCCAG AGTGAAGTGC CGTGAAGGTC 361 TCCACAGTTA GTTCCCGACA GCCCAGCACC ATTCCAGTGA TTGAGATCGG TCCGGCTGGA 421 GAATGAGTGG GAACAGTCGA GGGAGCCCAG CCGGCTCTGG CTGGACCCGA GAGACTGCCA 481 GCCGGGAGAA GGGGTCAGAG ACTGGCTTTG TGCAAAGAAA CGACTGATCT CCTCTTCACT 541 GGCAAACTCA GCAAGAATAG TAGTGTTCCC CAGTACACAC ATGTGCAGAG ACTTTTGTGC 601 CTTCACTACC TCTTCTTTTG AACTGTAGCG GACCAGAGCA TTTCCGTGAG GGAGGTTCAG 661 GTGGAATGTG ATCAGCGGGC CGTGCTGCAT GCACAGAGTG CGCAGAGTTG AGCCATCGAT 721 CTGAGGTGTA AGGTTTTTTT AGAACAAGCC AATTTGTTAT TCTCCCTGAG CTGCTCTCAC 781 CCCAGCTGGA ACCTGGGGGT ATATCTGGCG TCAGAATTTC CCCATCCCTC CACCTATACG 841 AAAGGGGAGA ATTATCCCAC GTAGACAAGG GTGGCTTTCT GACCAGTCAG TCCCGGGAGG 901 TGGGGCGGGA CGAGCAGTGA TGTTTTTTAG GTGGGCAAAG GACTTTCACA GCTCATGGAG 961 CCAGAAGGAG GTGTTTGTAA CTGACCAGAG ACATGTCATT GGATCACTAT TTCTGCCTGG 1021 ACTTGCTAGT GCTGTACTGC TTGAGGGAAG CCGGATG // |
Primer: BGHpA_F Sequence file: RDB14219_A6Fud.seq ![]() |
>D03279A7_A6Fu_1_BGHpA_F_D10_10.ab1 1 CGGGATGGTT ATCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG 61 AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA 121 CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 181 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 241 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 301 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 361 ATTAGGGTGA TGGTTCACGT ACCTAGAAGT TCCTATTCCG AAGTTCCTAT TCTCTAGAAA 421 GTATAGGAAC TTCCTTGGCC AAAAAGCCTG AACTCACCGC GACGTCTGTC GAGAAGTTTC 481 TGATCGAAAA GTTCGACAGC GTCTCCGACC TGATGCAGCT CTCGGAGGGC GAAGAATCTC 541 GTGCTTTCAG CTTCGATGTA GGAGGGCGTG GATATGTCCT GCGGGTAAAT AGCTGCGCCG 601 ATGGTTTCTA CAAAGATCGT TATGTTTATC GGCACTTTGC ATCGGCCGCG CTCCCGATTC 661 CGGAAGTGCT TGACATTGGG GAATTCAGCG AGAGCCTGAC CTATTGCATC TCCCGCCGTG 721 CACAGGGTGT CACGTTGCAA GACCTGCCTG AAACCGAACT GCCCGCTGTT CTGCAGCCGG 781 TCGCGGAGGC CATGGATGCG ATCGCTGCGG CCGATCTTAG CCAGACGAGC GGGTTCGGCC 841 CATTCGGACC GCAAAGGAAT CGGTCAATAC ACTACATGGC GTGATTTCAT ATGCGCGATT 901 GCTGATCCCC ATGTGTATCA CTGGCAAACT GTGATGGGAC GACACCGTCA GTGCGTCCGT 961 CGCGCAGCTC TCGATGAGCT GATGCTTTGG GCCGAAGACT GCCCCGAAGG TCAGCACCTC 1021 GTGCACGCGG AATTTCGCTC GACCATGTCT GACGGACATG ACGGCATAAC CAGCCGGGTT 1081 CAATTGG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. [link to RRC of NBRP] |
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